Efeito acaricida in vitro de extratos de plantas do Pantanal no carrapato de bovinos, Rhipicephalus (Boophilus) microplus.

Estudou-se a ação acaricida dos extratos brutos etanólicos de 14 plantas do Pantanal sobre fêmeas ingurgitadas do carrapato-do-boi. Os bioensaios foram realizados em triplicatas pelo método de imersão das teleóginas e estimadas a produção de ovos, a eclodibilidade e a eficácia dos extratos. Extratos do lenho da raiz e da casca da raiz de Annona dioica, da raiz e da casca do caule de Simarouba versicolor, da raiz de Annona cornifolia e de Duguetia furfuracea tiveram atividade acaricida entre 50% e 100% e resposta dose-dependente. Extratos de Dimorphandra mollis, Magonia pubescens, Protium heptaphyllum, Hyptis crenata, Sebastiana hispida, Aspidosperma australe, Senna occidentalis e de Elyonurus muticus mostraram atividade acaricida baixa ou ausente (0% a 10%). Extratos da parte aérea de A. cornifolia, da planta inteira de Croton glandulosus e da casca do caule de Stryphnodendron obovatum mostraram atividade acaricida intermediária (10% a 20%). A alta atividade acaricida observada nos extratos de A. dioica, A. cornifolia e D. furfuracea indica ação de acetogeninas, moléculas naturais com várias atividades farmacológicas já descritas e presentes, principalmente, nas plantas da família Annonaceae.

Estudio de los matorrales higrófilos (Genistion micrantho-anglicae) en la provincia de León

En el presente trabajo se estudian los brezales higrófilos leoneses que pertenecen a dos asociaciones: Genista anglicae-Ericetum tetralicis y Genisto anglicae-Ericetum vagantis. Se describe la Genisto anglicae-Ericetum tetralicis scirpetosum germanicii nova y las subasociaciones ericetosum tetralicis nova, genistetosum occidentalis nova y deschampietosum hispanicae nova, de la asociación Genisto anglicae-Ericetum vagantis.

Avances en el estudio de la reproducción del langostino

Estudio de desarrollo gonadal y reproducción de especies del género Penaeus. Los muestreos se realizaron en el litoral del departamento de Tumbes, en base a la captura de embarcaciones arrastreras comerciales. Se describe el desarrollo gonadal de P.occidentalis y P.stylirostris y P. vannamei y el seguimiento del desove y proceso larval hasta Protozoea I de P. occidentalis.

Biología y pesquería de cuatro especies de invertebrados marinos de importancia comercial. Región Tumbes, II Etapa – 2007

La Sede Regional de IMARPE Tumbes en el 2007 realizó la segunda etapa del estudio biológico pesquero de langosta (Panulirus gracilis), ostra (Ostrea iridescens), cangrejo de los manglares (Ucides occidentalis) y concha negra (Anadara tuberculosa). El desembarque de esas especies fue 432,9 t, constituyendo el 75% de los invertebrados marinos extraídos en esta Región. Se registró 11 especies (8 moluscos, 2 artrópodos y 1 equinodermo) relacionados con sus capturas. El cangrejo de los manglares alcanzó las mayores capturas con 220,5 t. El centro de acopio El Tumpis registró el mayor desembarque con 191,7 t (44,28%) y predominio de la ostra, Villar registró 119,3 t (27,56%). La mayor CPUE anual fue para ostra (48,5 kg/viaje) seguido por concha rayada (16,7 kg/faena) y el cangrejo de los manglares (9,1 kg/faena). Se reportó 70% de ejemplares de concha negra por debajo de la talla mínima legal de extracción (TME) (45 mm de longitud valvar), y 18% en cangrejo de los manglares (65 mm AC).

« Mémoires du droit particulier du Roy sur les villes et chastellenies de Dunquerque, Bourbourg et Gravelines », sur la « dignité du chastelain de Lille en Flandre, et les droits qui appartiennent au Roy en ceste qualité », comprenant une « généalogie des seigneurs possesseurs de ces villes et chastelains jusques à présent, avec le mémoire des terres alliénées aux Pays-Bas de l'ancien domaine de Navarre », par Georges GALLAND.

En 22 chapitres. — Aux feuillets 5-6, carte gravée : « Flandriae pars occidentalis... ; Amstelodami, sumptibus Henrici Hondii. » — Aux feuillets 46-47, autre carte gravée : « Flandria gallica, continens castellanias Insulensem, Duacensem, Orchianensem, civitatem dominiumque Tornacense... ; Amstelodami, Joannes Janssonius. » — Les feuillets 7-9, 59, 60, et les notes marginales sont de la main de Georges Galland. Table des chapitres en tête du volume.

Abrégé « de l'histoire du regne d'Henry VII, roy d'Angleterre, de messire François BACON ».

Contient : « Collectanea ex historia Indiae occidentalis et Terrae novae. — Avenione, 13° augusti anno 1608 »

Le bois d'oeuvre et le port de Montréal, 1830-1870 : une approche dendroarchéologique

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Grasshoppers and crickets (Insecta : Orthoptera) from the lower cretaceous of southern England

This is a synoptic monograph of fossil Orthoptera from the English Lower Cretaceous (Purbeck and Wealden groups). The previously described taxa of these insects are revised on the basis of type specimens and examination of extensive new material. Eight new genera and 30 new species are proposed: Probaisselcana cretacea sp. nov., Minelcana membranacea gen. et sp. nov., Panorpidium proximum sp. nov., P. bimacillatum sp. nov., ?P. parvum sp. nov. (Elcanidae); ?Cyrtophyllites cretaceus sp. nov. (Haglidae); Aenigmodus minutus gen. et sp. nov., Pseudaboilus wealdensis gen. et sp. nov., P. purbeckensis sp. nov., Tettigoilus sonorus gen. et sp. nov., ?Agrionidium obscurum sp. nov. (Prophalangopsidae); Notocearagryllus britannicus sp. nov., N. grandispeculum sp. nov., N. cordispeculum sp. nov., Anglogryllus lyristes gen. et sp. nov., A. rotundispeculum sp. nov.. Speculogryllus acutispeculum gen. et sp. nov., Sharategia davisi sp. nov., S. batchelorae sp. nov., S. baldocki sp. nov. (Baissogryllidae); ?Araripegryllus orientalis sp. nov. (Gryllidae); Deinovitimia occidentalis sp. nov. (Ensifera: infraorder incertae sedis); Cretoxya rasnitsyni gen. et sp. nov. (Tridactylidae); Locustopsis posterior sp. nov., Zeunerella prior sp. nov., Zessinia borealis sp. nov., Mesolocustopsis anglica sp. nov., M. angusta sp. nov., M. problematica sp. nov., and Britannacrida distincta gen. et sp. nov (Locustopsidae). The subfamily Baisselcaninae is synonymized with Elcaninae, and a new subfamily (Archelcaninae subfam. nov.) is proposed for a segregate of Elcaninae. A preliminary comparison of the Purbeck/Wealden with other Early Cretaceous orthopteran faunas is given. (c) 2006 Published by Elsevier Ltd.

Temperature and the development rates of thrips: evidence for a constraint on local adaptation?

Typically, the relationship between insect development and temperature is described by two characteristics: the minimum temperature needed for development to occur (T-min) and the number of day degrees required (DDR) for the completion of development. We investigated these characteristics in three English populations of Thrips major and T tabaci [Cawood, Yorkshire (N53degrees49', W1degrees7'); Boxworth, Cambridgeshire (N52degrees15', W0degrees1'); Silwood Park, Berkshire (N51degrees24', W0degrees38')], and two populations of Frankliniella occidentalis (Cawood; Silwood Park). While there were no significant differences among populations in either T-min (mean for T major = 7.0degreesC; T tabaci = 5.9degreesC; F. occidentalis = 6.7degreesC) or DDR (mean for T major = 229.9; T tabaci = 260.8; F occidentalis = 233.4), there were significant differences in the relationship between temperature and body size, suggesting the presence of geographic variation in this trait. Using published data, in addition to those newly collected, we found a negative relationship between T-min. and DDR for F occidentalis and T tabaci, supporting the hypothesis that a trade-off between T-min and DDR may constrain adaptation to local climatic conditions.

Ecto- and arbuscular mycorrhizal symbiosis can induce tolerance to toxic pulses of phosphorus in jarrah (Eucalyptus marginata) seedlings

In common with many plants native to low P soils, jarrah (Eucalyptus marginata) develops toxicity symptoms upon exposure to elevated phosphorus (P). Jarrah plants can establish arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) associations, along with a non-colonizing symbiosis described recently. AM colonization is known to influence the pattern of expression of genes required for P uptake of host plants and our aim was to investigate this phenomenon in relation to P sensitivity. Therefore, we examined the effect on hosts of the presence of AM and ECM fungi in combination with toxic pulses of P and assessed possible correlations between the induced tolerance and the shoot P concentration. The P transport dynamics of AM (Rhizophagus irregularis and Scutellospora calospora), ECM (Scleroderma sp.), non-colonizing symbiosis (Austroboletus occidentalis), dual mycorrhizal (R. irregularis and Scleroderma sp.), and non-mycorrhizal (NM) seedlings were monitored following two pulses of P. The ECM and A. occidentalis associations significantly enhanced the shoot P content of jarrah plants growing under P-deficient conditions. In addition, S. calospora, A. occidentalis, and Scleroderma sp. all stimulated plant growth significantly. All inoculated plants had significantly lower phytotoxicity symptoms compared to NM controls 7 days after addition of an elevated P dose (30 mg P kg−1 soil). Following exposure to toxicity-inducing levels of P, the shoot P concentration was significantly lower in R. irregularis-inoculated and dually inoculated plants compared to NM controls. Although all inoculated plants had reduced toxicity symptoms and there was a positive linear relationship between rank and shoot P concentration, the protective effect was not necessarily explained by the type of fungal association or the extent of mycorrhizal colonization.

A novel plant-fungus symbiosis benefits the host without forming mycorrhizal structures

Most terrestrial plants form mutually beneficial symbioses with specific soil-borne fungi known as mycorrhiza. In a typical mycorrhizal association, fungal hyphae colonize plant roots, explore the soil beyond the rhizosphere and provide host plants with nutrients that might be chemically or physically inaccessible to root systems. Here, we combined nutritional, radioisotopic (33P) and genetic approaches to describe a plant growth promoting symbiosis between the basidiomycete fungus Austroboletus occidentalis and jarrah (Eucalyptus marginata), which has quite different characteristics. We show that the fungal partner does not colonize plant roots; hyphae are localized to the rhizosphere soil and vicinity and consequently do not transfer nutrients located beyond the rhizosphere. Transcript profiling of two high-affinity phosphate (Pi) transporter genes (EmPHT1;1 and EmPHT1;2) and hyphal-mediated 33Pi uptake suggest that the Pi uptake shifts from an epidermal to a hyphal pathway in ectomycorrhizal plants (Scleroderma sp.), similar to arbuscular mycorrhizal symbioses, whereas A. occidentalis benefits its host indirectly. The enhanced rhizosphere carboxylates are linked to growth and nutritional benefits in the novel symbiosis. This work is a starting point for detailed mechanistic studies on other basidiomycete–woody plant relationships, where a continuum between heterotrophic rhizosphere fungi and plant beneficial symbioses is likely to exist.

Dual mycorrhizal associations of jarrah (Eucalyptus marginata) in a nurse-pot system

Jarrah (Eucalyptus marginata Donn ex Sm.) plants, like many other eucalypts, can form symbiotic associations with both arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) fungi. To study this tripartite relationship we developed a novel nurse-pot system to allow us to investigate the extent and temporal colonisation dynamics of jarrah by two AM species (Rhizophagus irregularis (Błaszk., Wubet, Renker & Buscot) C. Walker & A. Schüßler comb. nov. and Scutellospora calospora Nicol. & Gerd.) and two putative ECM species (Austroboletus occidentalis Watling & N.M. Greg. and Scleroderma sp.) and their potential effects on jarrah growth and nutrition. Our nurse-pot system, using jarrah as both the nurse plant and test plant, was developed to establish extraradical hyphal networks of both AM and ECM fungi that act as single or dual inoculum for test plants. Mycorrhizal colonisation was described and quantified, and growth and nutritional effects measured and analysed. Mycorrhizal colonisation increased with time for the test seedlings exposed to hyphae networks from S. calospora and Scleroderma sp. The nurse-pot system was effective at initiating colonisation of functioning AM or (putative) ECM systems separately but the ECM symbiosis was inhibited where a dual AM + ECM inoculum (R. irregularis and Scleroderma sp.) was present. The presence of S. calospora, A. occidentalis and Scleroderma sp. individually significantly increased the shoot biomass of seedlings compared with non-mycorrhizal controls. The two AM isolates had different physiological effects on jarrah plants. S. calospora improved growth and micronutrient uptake of jarrah seedlings whereas no positive response was observed with R. irregularis. In addition, as an interesting observation, the non-responsive AM fungus R. irregularis suppressed the ECM symbiosis in dually inoculated plants where ECM structures, positive growth response and nutritional effects were absent. When inoculated individually, ECM isolates dominated the growth response and uptake of P and other nutrients in this dual symbiotic plant. Despite the positive growth response in the A. occidentalis treatment, ECM structures were not observed in either nurse or test seedlings. From the effects of A. occidentalis on jarrah we hypothesise that this fungus forms a functional mycorrhizal-type partnership even without forming archetypal structures in and on the root

Reforesting degraded agricultural landscapes with Eucalypts: effects on carbon storage and soil fertility after 26years

In the Western Australian wheatbelt, the restoration of native eucalypt forests for managing degraded agricultural landscapes is a critical part of managing dryland salinity and rebuilding biodiversity. Such reforestation will also sequester carbon. Whereas most investigative emphasis has been on carbon stored in biomass, the effects of reforestation on soil organic carbon (SOC) stores and fertility are not known. Two 26 year old reforestation experiments with four Eucalyptus species (E. cladocalyx var nana, E. occidentalis, E. sargentii and E. wandoo) were compared with agricultural sites (Field). SOC stores (to 0.3 m depth) ranged between 33 and 55 Mg ha−1, with no statistically significant differences between tree species and adjacent farmland. Farming comprised crop and pasture rotations. In contrast, the reforested plots contained additional carbon in the tree biomass (23–60 Mg ha−1) and litter (19–34 Mg ha−1), with the greatest litter accumulation associated with E. sargentii. Litter represented between 29 and 56% of the biomass carbon and the protection or utilization of this litter in fire-prone, semi-arid farmland will be an important component of carbon management. Exch-Na and Exch-Mg accumulated under E. sargentii and E. occidentalis at one site. The results raise questions about the conclusions of SOC sequestration studies following reforestation based on limited sampling and reiterate the importance of considering litter in reforestation carbon accounts.

Bioprospecção de extratos metanólicos de macroalgas marinhas do litoral do Rio Grande do Norte: avaliação das atividades anticoagulante, antioxidante e antiproliferativa

Seaweeds are organisms known to exhibit a variety of biomolecules with pharmacological properties. The coast of Rio Grande do Norte has over 100 species of seaweeds, most of them not yet explored for their pharmacological potential. Sugars and phenolic compounds are the most studied of these being assigned a range of biological properties, such as anticoagulant , antiinflammatory, antitumor and antioxidant activities. In this work, we obtained methanolic extracts from thirteen seaweeds of the coast of Rio Grande do Norte (Dictyota cervicornis; Dictiopterys delicatula; Dictyota menstruallis; D. mertensis; Sargassum filipendula; Spatoglossum schröederi; Acanthophora specifera; Botryocladia occidentalis; Caulerpa cupresoides; C. racemosa; C. prolifera; C. sertularioides e Codium isthmocladum). They were evaluated as anticoagulant and antioxidant drugs, as well as antiproliferative drugs against the tumor cell line HeLa. None of the methanolic extracts showed anticoagulant activity, but when they were evaluated as antioxidant drugs all of extracts showed antioxidant activity in all tests performed (total antioxidant capacity, sequestration of superoxide and hydroxyl radicals, ferric chelation and reductase activity), especially the algae D. mentrualis, D. cilliolata and C. prolifera, who had the greatest potential to donate electrons.In addition, the ability of iron ions chelation appears as the main antioxidant mechanism of the methanolic extracts of these seaweeds mainly for the extract of the C. racemosa seaweed, which reached almost 100% activity. In the MTT assay, all extracts showed inhibitory activity at different levels againts HeLa cells. Moreover, D. cilliolata (MEDC) and D. menstrualis (MEDM) extracts showed specific activity to this cell line, not inhibiting the viability of 3T3 normal cell line, so they were chosen for detailing the antiproliferative mechanism of action. Using flow cytometry, fluorescence microscopy and in vitro assays we demonstrated that MEDC and MEDM induced apoptosis in HeLa cells by activation of caspases 3 and 9 and yet, MEDC induces cell cycle arrest in S phase. Together, these results showed that the methanolic extracts of brown seaweed D. menstrualis and D. cilliolata may contain agents with potential use in combatting cells from human uterine adenocarcinoma. This study also points to the need for more in-depth research on phytochemical and biological context to enable the purification of biologically active products of these extracts

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)