Tipos de miosinas de linhagens de frangos de corte criados em sistemas de confinamento e semiconfinamento

O objetivo neste trabalho foi avaliar o peso vivo, o peso de pernas, os aspectos morfológicos das fibras musculares do músculo flexor longo do hálux e o perfil eletroforético das miosinas de cadeia pesada de quatro linhagens de frangos de corte criados nos sistemas de confinamento e semiconfinamento. Foram utilizados 1.440 pintos distribuídos em delineamento inteiramente casualisado em esquema fatorial 4 × 2, composto de quatro linhagens (Ross 305, Máster Gris, Label Rouge e Vermelhão Pesado) e dois sistemas de criação (confinamento e semiconfinamento), cada combinação avaliada com quatro repetições. Aos 28 e 84 dias de idade, foram abatidas quatro aves por tratamento, totalizando 64 aves. A eletroforese identificou a presença das três isoformas de miosinas, tipo MyHC-I, MyHC-IIa e MyHC-IIb, no músculo flexor longo do hálux dos frangos de corte. Com aumento da idade, a isoforma de miosina MyHC-II aumenta, enquanto a MyHC-I diminui. Somente aos 84 dias de idade, a expressão das isoformas de miosina do tipo MyHC-II foram influenciadas pela linhagem, confirmando o reflexo da seleção na linhagem Ross no músculo mais glicolítico.A linhagem Ross apresenta maior peso vivo, peso de perna, peso e área do músculo flexor longo do hálux em comparação às linhagens tipo caipira.

Pressão Arterial e Reatividade Vascular em Frangos de Corte Machos de Cinco Linhagens Comerciais

Blood pressure and vascular reactivity to phenylephrine (hypertensor) and sodium nitroprusside (hypotensor) was determined on male broilers taken from 5 commercial strains (Arbor Acres, Cobb, Hubbard-Peterson, ISA and Ross), with 21-28 days of age. Blood pressure was measured in the femoral artery by introducing a cannula attached to a pressure transdutor and recorded on a polygraph. Hyper or hypopressor substances were injected via jugular vein at 5, 10, 20 and 40-mcg kg(-1) body weight. No differences in the systolic, diastolic and mean blood pressure and no significant blood pressure responses to phenylephrine and sodium nitroprusside were observed among strains. Throughout strain and treatment blood pressures (systolic, diastolic and mean) were high in both experiments. This suggests that these modern male broilers have high arterial pressure possibly due to an indirect selection effect.

Diversidade genética de quatro linhagens de Oreochromis niloticus utilizando o marcador RAPD

The aim of this study was to analyze the genetic diversity of four Nile Tilapia (Oreochromis niloticus) strains using the RAPD marker. Fin samples of GIFT (G), Chitralada (C), Supreme (S) and Bouake (B) juvenile stocks have been collected. The 11 primers used yielded 81 fragments of which 41.98% were polymorphic. The percentage of polymorphic loci (G: 18.52%; C: 19.75%; S: 20.99% and B: 24.79%) showed that there was a genetic differentiation among the strains, showing the G(st) values a high (BxG: 0.231; BxC: 0.224; GxC: 0.194 and SxC: 0.208) and elevated (BxS: 0.315 and GxS: 0.270) differentiation. The highest gene flow (N(m)) was among the GxC (2.082) strains. The distance and genetic identity values (0.044 and 0.957 respectively) and the dendrogram indicate that the GxC is the most genetically similar strains. The genetic similarity was high among of the strains (G: 0,932; C: 0,903; S: 0,891 and B: 0.900). These results will enable a correct reproductive and genetic strains management.

CARCINO-SARCOMA 256 DE WALKER: DISSEMINACAO METASTATICA EM DUAS LINHAGENS DO TUMOR

Walker's 256 carcinoma changes its behaviour as a consequence of various factors. In this paper the authors compare the evolution of 2 lines of the tumor: WM 16 (muscular) and Christ Hospital (ascitic) both inoculated intramuscularly. Animals receiving line WM 16 had a severe rapidly progressive evolution dying around day 14 after inoculation with diffuse metastases to lymph nodes (65% of animals), kidneys (53%), spleen (50%), lungs (46.5%), liver (45%), bone marrow (44.8%), in 56% of the animals there were circulating tumoral cells. Animals receiving the Christ Hospital line survived up to 40 days, metastases were limited to lungs (48.7%) and lymph nodes (31.7%) and only in 2 of 45 animals circulating tumoral cells were observed.

Efeito da seleção quanto ao peso corporal nas características reprodutivas em linhagens de frangos de corte

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Características físicas e fisiológicas na qualidade industrial de cultivares e linhagens de trigo e triticale

The physical, physiological and rheological characteristics of cultivars and lines of wheat CD (COODETEC) 103, 104, 105, 107, 108, 109, 110, 111 and CD 2017 and CDFAPA 2036 lines and triticale (EMBRAPA 53), for quality of flour for bread making were studied. The weight of hectliter (HW), weight of thousand grains (WTG), percentage of germination, total protein and farinography, in a completely randomized experimental design, with four repetitions, except the farinography (with two repetitions) were analyzed. The means obtained were compared by the Scott Knott test at 5%. The cultivars were different regarding the tested parameters. The PH value were compatible for commercialization, but not always the higher PH value corresponded to the greater weight of thousand grains. Only the CD 108 and 111 cultivars may be destine for French bread considering the stability parameter. About the index of tolerance for the mixture, the cultivars and lines were not different, but only the flour of 2017 line would attend the values that were established for French bread-making and fermented cookies. The CD 104, 108 and 11 cultivars and the CD 2017 and CDFAPA 2036 line obtained the ideal index also for fermented cookies. The cultivars which presented better weight of thousand grain gave the best index of tolerance for the mixture. There was no positive relation between total protein and the farinography.

Influência de protocolos de jateamento na rugosidade da superfície de uma cerâmica de zircônia tetragonal parcialmente estabilizada por ítria

Objective: To evaluate the influence of different air abrasion protocols on the surface roughness of an yttria-stabilized polycrystalline tetragonal zirconia) (Y-TZP) ceramic, as well as the surface topography of the ceramic after the treatment. Method: Fifty-four specimens (7.5×4×7.5mm) obtained from two ceramic blocks (LAVA, 3M ESPE) were flattened with fine-grit sandpaper and subjected to sintering in the ceramic system's specific firing oven. Next, the specimens were embedded in acrylic resin and the surfaces to be treated were polished in a polishing machine using sandpapers of decreasing abrasion (600- to 1,200-grit) followed by felt discs with 10μm and 3μm polishing pastes and colloidal silica. The specimens were then randomly assigned to 9 groups, according to factors particle and pressure(n=6): Gr1- control; Gr2- Al 2O 3(50μm)/2.5 bar; Gr3- Al 2O 3(110μm)/2.5 bar; Gr4- SiO 2(30μm)/2.5 bar; Gr5- SiO 2(30μm)/2.5 bar; Gr6- Al 2O 3(50μm)/3.5 bar; Gr7- Al2O3(110μm)/3.5 bar; Gr8- SiO 2(30μm)/3.5 bar; Gr9- SiO 2(30μm)/3.5 bar. After treatments, surface roughness was analyzed by a digital optical profilometer and the morphology was examined by scanning electron microscopy (SEM). Data (μm) were subjected to statistical analysis by Dunnett's test (5%), two-way ANOVA and Tukey's test (5%). Results: The type of particle (p=0.0001) and the pressure (p=0.0001) used in the air abrasion protocols influenced the surface roughness values among the experimental groups (ANOVA). The mean surface roughness values (μm) obtained for the experimental groups (Gr2 to Gr9) were, respectively: 0.37 D; 0.56 BC; 0.46 BC; 0.48 CD; 0.59 BC; 0.82 A; 0.53B CD; 0.67 AB. The SEM analysis revealed that Al 2O 3, regardless of the particle size and pressure used, caused damage to the surface of the specimens, as it produced superficial damages on the ceramic, in the form of grooves and cracks. Conclusion: Al2O3 (110 μm/3.5 bar) air abrasion promoted the highest surface roughness on the ceramics, but it does not mean that this protocol promotes better ceramic-cement union compared to the other air abrasion protocols.

Análise comparativa da produtividade de híbridos de mamoneira (Ricinus communis L.) obtidos por meio da hibridação convencional e do método de híbridos crípticos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Expressão sexual em linhagens de mamona (Ricinus communis L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de linhagens da população FCA planta baixa de mamona (Ricinus communis L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desempenho de alevinos de quatro linhagens da tilápia do Nilo (Oreochromis niloticus) e análise da variabilidade genética pelos marcadores RAPD

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desempenho produtivo e crescimento muscular de linhagens de tilápia-do-nilo cultivadas na fase inicial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise da expressão gênica de MyoD, MRF4, miogenina e miostatina nos músculos Bíceps femoris e Gastrocnemius lateralis em duas linhagens de Gallus gallus (corte e postura)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise filogenética de linhagens de Xylella fastidiosa de diferentes hospedeiros baseada na sequencia da região intergênica 16S-23S rDNA

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Estudo comparativo in vitro da suscetibilidade à ciprofloxacina e à vancomicina em linhagens de Enterococcus faecalis isoladas de processos infecciosos

Pós-graduação em Doenças Tropicais - FMB