Experiment on marine fungus Microascus brevicaulis strain

The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularide A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing besides higher production levels faster growth and differences in pellet formation. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of this fungus and its mutant. For this purpose, an optimised protein extraction protocol was established. Here, we show the first proteome study of a marine fungus. In total, 4759 proteins were identified. The central metabolic pathway of LF580 could be mapped by using KEGG pathway analysis and GO annotation. Using iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to a limited nutrient availability in wild type strain due to a strong pellet formation. This information can be applied to optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum.

Modelo de integración del conocimiento-acción a través del Proyecto de Comunicación para el Desarrollo: 25 años de experiencia en comunidades indígenas andinas en Ecuador

El desarrollo como política de las naciones marca el período de la postguerra, que en América Latina se concreta en múltiples programas de Desarrollo Rural. El modelo inicial de crecimiento económico se va enriqueciendo con visiones centradas en el ser humano y sus múltiples concepciones de desarrollo. En este entender el desarrollo desde la diversidad cultural y antropológica de los pueblos de América Latina, surge la comunicación radiofónica como camino común para la persuasión, socialización y apropiación colectiva de conocimientos y ámbito participativo para consensuar objetivos y acordar actividades. El activismo de los años setenta y ochenta produjo un número considerable de experiencias. La mayoría de ellas han llegado hasta nuestros días, pero el giro tomado por las sociedades digitalizadas y las prioridades medioambientales han dado un nuevo rostro a aquellas primeras experiencias centradas en el desarrollo rural endógeno. Este estudio investiga el complejo práctico-teórico que integra comunicación y desarrollo en contextos territoriales interculturales con población marginal y aplicación de políticas de desarrollo rural. En el capítulo I se recogen las modalidades de cómo se ha aplicado la comunicación en programas de desarrollo, para profundizar en el concepto de comunicación para el desarrollo, conocer sus actores y resultados, y concluir que el desarrollo “con rostro humano” se hace con personas no con políticas oficiales. El capítulo II presenta a los Pueblos Indígenas de la Sierra Ecuatoriana como actores de su proceso de desarrollo hacia el Sumak Kawsay. Los temas tratados en este capítulo muestran los valores y capacidades de las comunidades indígenas de la Sierra Ecuatoriana para la autogestión de su desarrollo, y deja constancia y denuncia de la lectura negativa que se ha venido haciendo de su cosmovisión. Se encuentra respuesta conceptual y metodológica en el Workng With People para optimizar aportes culturales de los Pueblos Indígenas al proceso comunicacional y participativo para mejorar las condiciones de vida y lograr mayores espacios de autonomía y libertad El conocimiento que explica la realidad sin cambiarla no tiene sentido para los pobres. La realidad requiere ser comprendida para construir el conocimiento que la explique y la cambie: ese es el aporte del modelo conocimiento/acción que se presenta en el capítulo III. En este capítulo se exponen metodologías abiertas a la creatividad y flexibles que responden a los principios del modelo conocimiento/acción. La comunicación para el desarrollo se abre a todas ellas como ágora pluricultural que requiere un lenguaje común que se construye desde el aprendizaje social. Los conceptos expuestos en el capítulo III se vinculan en una propuesta metodológica que integra el conocimiento y la acción con los principios del Working With People, propuesta que se expone en el capítulo IV. El modelo del Proyecto de Comunicación para el Desarrollo junta en un modelo de comunicación popular y prácticas liberadoras con métodos cognitivos de la realidad para planificar el cambio desde la población y con la población. Finalmente, el capítulo V recoge la experiencia de la Casa Campesina Cayambe ejecutora del Proyecto de Comunicación para el Desarrollo en la adaptación ejecutada con Radio Mensaje. La riqueza de la experiencia tiende a escaparse de los límites conceptuales y los esquemas dejan espacios de vida ignotos; pero los conceptos y esquemas constituyen el camino para que la experiencia pase a ser objeto conocido y conocimiento socializado. El Proyecto de Comunicación para el Desarrollo es resultado de un proceso de desarrollo construido desde la acción de la Casa Campesina Cayambe durante 25 años en Cayambe. En el capítulo V se describe el contexto del territorio de Cayambe, la identidad de la Casa Campesina Cayambe y de Radio Mensaje, y termina describiendo los logros de 25 años trabajando con la gente. El estudio concluye que la comunicación participativa, como ámbito de aprendizaje social aplicado en procesos de desarrollo rural, integra el conocimiento/acción e incorpora nuevos conocimientos en la población con los que se desarrollan competencias locales para planificar el desarrollo endógeno con flexibilidad y de abajo-arriba. La Figura 0-1 grafica los elementos bases sobre los que se construye el proceso de desarrollo. ABSTRACT Development as a policy of nations marks the postwar period which created several Rural Development Programs in Latin America. The initial model of economic growth was enriched with approaches focused on human beings and their multiple conceptions of development. In this regard, the development from cultural and anthropological diversity in Latin America, radio communication emerges as a common means for persuasion, socialization and collective appropriation of knowledge and scope for participation in order to agree on objectives and activities. Activism of the seventies and eighties generated a considerable number of experiences, most of which are present today. However, a turn taken by digitalized societies and environmental priorities has given those first experiences which focused on endogenous rural development a new face. This study researches the theoretical-practical status that integrates communication and development in intercultural contexts with marginal population and the use of rural development policies. Chapter one shows the modalities of how communication in development programs have been applied to deepen the concept of communication for development, to know those who are involved and its findings, and conclude that development “with a human face” is done with people not with official policies. Chapter two presents Indigenous communities in the Ecuadorian highlands, as people involved in their development process towards Sumak Kawsay. The topics in this chapter show the values and capacities indigenous communities in the Ecuadorian highlands have to self-manage their development, and proves, as well as denounces, that their cosmovision has been negatively perceived. A conceptual and methodological response is found in Working With People in order to optimize cultural contributions of Indigenous People to the communicational and participative process to improve life conditions and have greater spaces of autonomy and freedom. Knowledge which explains reality without changing it does not make any sense for the poor. Reality need to be understood in order to build the knowledge that will explain it and change it: that is the contribution of the knowledge/action model presented in chapter three. This chapter presents open methodologies to creativity which are flexible to respond to the principles of the knowledge/action model. Communication for development is open to all of them as pluricultural agora which requires a common language that is built from social learning. The concepts presented in chapter three are linked in a methodological proposal which integrates knowledge and action with principles of Working With People, proposal which is presented chapter four. The model of the Communication for Development Project includes popular communication elements and liberating practices with cognitive methods of reality to plan change from the population and with the population. Finally, chapter five presents the experiences from the Cayambe Country House, which conducted the Communication for Development Project in the adaptation implemented with Radio Mensaje. The wealth of experience tends to scape from the conceptual limits and the schemes leave gaps of unexplored life; but the concepts and schemes constitute the way so that experience becomes a known object and socialized knowledge. The Communication for Development Project is the result of a development process built from the actions of the Cayambe Country House during 25 years in Cayambe. Chapter five describes the context of Cayambe, the identity of the Cayambe Country House and Radio Mensaje, and the achievements after 25 years of working with its people. The study concludes that participatory communication, as an area of social learning applied to rural development processes, integrates knowledge/action and incorporates new knowledge in communities to develop local competencies to plan endogenous development with flexibility and from the bottom – up.

Towards a U.S. Army officer corps strategy for success : employing talent /

Efficient talent employment is at the core of the Army Officer Human Capital Model. However, the Army's current employment paradigm is unequal to the needs of a professional, volunteer Army facing the twin challenges of a competitive labor market and an increasingly complex global operating environment. It unduly prioritizes "fairness" when making assignments, has a narrowly defined pathway to senior leadership ranks, cannot see the talent it possesses, and suffers from severe principal-agent problems. Optimal employment theories, information age tools, and well-regulated market mechanisms can help the Army match individual officer talents against specific work requirements, reducing risk and achieving the depth and breadth of talent it needs, both now and in the future.

Information and Communication Technology (ICT) international spillovers

As information and communications technology (ICT) involves both traditional capital and knowledge capital, potential spillovers through various mechanisms can occur. Having tried to confirm the existence of ICT spillovers across country borders as Park et al. (Inf. Syst. Res., vol. 18, pp. 86-102, 2007), we investigate the patterns and mechanisms of international ICT spillovers. We use panel data on 37 countries from 1996 to 2004. We find that developing countries could reap more benefits from ICT spillovers than developed countries. We also find that the higher the Internet penetration rate in recipient countries, the more international ICT spillovers there might exist. Our findings are important for policy decisions regarding national trade liberalization and economic integration. Developing economies that are more open to foreign trade may have an economic advantage and may develop knowledge-intensive activities, which will lead to economic development in the long run.

Externality effects of information and communication technologies across country Borders

As information and communications technology (ICT) involves both traditional capital and knowledge capital, potential spillovers through various mechanisms can occur. We posit that ICT capital may boost productivity growth, not only in the home country, but also in other countries. In this paper, we provide empirical evidence of such spillovers using panel data on 37 countries from 1996 to 2004. Our results support the existence of ICT spillovers across country borders. Furthermore, we find that developing countries could reap more benefits from ICT spillovers than developed countries. This is particularly important for policy decisions regarding national trade liberalization and economic integration. Developing economies that are more open to foreign trade may have an economic advantage and may develop knowledge-intensive activities, which will lead to economic development in the long run.

A túl- és az alulképzés bérhozama 25 európai országban (Returns for over-education and under-education for 25 European countries)

A tanulmányban 25 ország, kétezres évek közepi állapotot tükröző, reprezentatív keresztmetszeti mintáin egyrészt a Duncan-Hoffman-féle modellre támaszkodva megvizsgáljuk, hogy adatbázisunk milyen mértékben tükrözi az illeszkedés bérhozamával foglalkozó irodalom legfontosabb empirikus következtetéseit, másrészt - a Hartog- Oosterbeek-szerzőpáros által javasolt statisztikai próbák segítségével - azt elemezzük, hogy a becslések eredményei alapján mit mondhatunk Mincer emberitőke-, valamint Thurow állásversenymodelljének érvényességéről. Heckman szelekciós torzítást kiküszöbölő becslőfüggvényén alapuló eredményeink jórészt megerősítik az irodalomban vázolt legfontosabb empirikus sajátosságokat, ugyanakkor a statisztikai próbák az országok többségére nézve cáfolják mind az emberi tőke, mind az állásverseny modelljének empirikus érvényességét. / === / Using the Duncan–Hoffman model, the paper estimates returns for educational mismatch using comparable micro data for 25 European countries. The aim is to gauge the extent to which the main empirical regularities shown in other papers on the subject are confirmed by this data base. Based on tests proposed by Hartog and Oosterbeek, the author also considers whether the observed empirical patterns accord with the Mincerian basic human-capital model and Thurow's job-competition model. Heckman's sample-selection estimator shows the returns to be fairly consistent with those found in the literature; the job-competition model and the Mincerian human-capital model can be rejected for most countries.

Modelo de gestão do conhecimento para a unidade organizacional de saúde

Nos últimos anos têm surgido vários debates e estudos sobre a importância do conhecimento. As organizações consideram a Gestão do Conhecimento (GC) uma vantagem competitiva, capaz de gerar riqueza e poder. Para tal necessitam de desenvolver mecanismos e de ter pessoas com capacidade de criar, compartilhar e disseminar conhecimentos na organização. As Tecnologias de Informação e os Sistemas de Informação são uma ferramenta de grande impacto na GC, pois assumem um papel importante no sucesso e na renovação dos conhecimentos. A partir do Modelo de GC desenvolvido por Nonaka e Takeuchi, o Modelo Metavisão, e as técnicas de Business lntelligence, elaborou-se um Modelo de Gestão do conhecimento para a Unidade de Saúde. Com este modelo pretende-se obter beneficies, que passam pelo desenvolvimento de mecanismos de comunicação interna, formação e melhorias no processo de tomada de decisão. ABSTRACT; During the last years, debates and studies have arisen in relation to the importance of knowledge. The organizations consider Knowledge Management a competitive advantage, capable of generating wealth and power. Therefore, new mechanisms have to be developed in the organizations and employ people with capacity to create, share and disseminate knowledge. The information and Communication Technology is an important tool and has a great impact on Knowledge Management because it assumes an important role in the success and the renovation of knowledge. The Model of Knowledge Management developed by Nonaka and Takeuchi, the "Metavision" Model and the techniques of Business intelligence were the starting point to elaborate a Model of Knowledge Management for a Health Unit. With this model we aim to obtain benefits, such as development of mechanisms of internal communication, training plans and improvement during the process of decision making.

Modelo para gestionar el conocimiento en el sector textil de Medellín, empleando dinámica de sistemas

Este artículo evalúa la relación de causalidad entre la gestión del conocimiento y las capacidades de innovación tecnológica, y el efecto de esta relación sobre los resultados operacionales del sector textil en la ciudad de Medellín. Se empleó la metodología de dinámica de sistemas, con simulación de esce­narios para valorar las condiciones actuales de las organizaciones del sector en términos de acumulación de conocimiento y capacidades. La información se obtuvo mediante entrevistas a expertos y acceso a información especializada del sector. Se evidencia que una mejora de la relación entre la gestión del conocimiento e innovación tecnológica genera un incremento aproximado del 15% en los ingresos operacionales del sector. Asimismo, se encontró que a medida que las variables comunes de interés (Es­trategias organizacionales, canales de comunicación, formación, cultura, acciones de fortalecimiento en I+D), se acercan a los valores deseados, la acumulación de conocimiento y de capacidades de innovación tecnológica alcanzan los valores objetivos.

An exploration of knowledge management and intellectual capital in a nonprofit organisation context

In recent years, practitioners and researchers alike have turned their attention to knowledge management (KM) in order to increase organisational performance (OP). As a result, many different approaches and strategies have been investigated and suggested for how knowledge should be managed to make organisations more effective and efficient. However, most research has been undertaken in the for-profit sector, with only a few studies focusing on the benefits nonprofit organisations might gain by managing knowledge. This study broadly investigates the impact of knowledge management on the organisational performance of nonprofit organisations. Organisational performance can be evaluated through either financial or non-financial measurements. In order to evaluate knowledge management and organisational performance, non-financial measurements are argued to be more suitable given that knowledge is an intangible asset which often cannot be expressed through financial indicators. Non-financial measurement concepts of performance such as the balanced scorecard or the concept of Intellectual Capital (IC) are well accepted and used within the for-profit and nonprofit sectors to evaluate organisational performance. This study utilised the concept of IC as the method to evaluate KM and OP in the context of nonprofit organisations due to the close link between KM and IC: Indeed, KM is concerned with managing the KM processes of creating, storing, sharing and applying knowledge and the organisational KM infrastructure such as organisational culture or organisational structure to support these processes. On the other hand, IC measures the knowledge stocks in different ontological levels: at the individual level (human capital), at the group level (relational capital) and at the organisational level (structural capital). In other words, IC measures the value of the knowledge which has been managed through KM. As KM encompasses the different KM processes and the KM infrastructure facilitating these processes, previous research has investigated the relationship between KM infrastructure and KM processes. Organisational culture, organisational structure and the level of IT support have been identified as the main factors of the KM infrastructure influencing the KM processes of creating, storing, sharing and applying knowledge. Other research has focused on the link between KM and OP or organisational effectiveness. Based on existing literature, a theoretical model was developed to enable the investigation of the relation between KM (encompassing KM infrastructure and KM processes) and IC. The model assumes an association between KM infrastructure and KM processes, as well as an association between KM processes and the various levels of IC (human capital, structural capital and relational capital). As a result, five research questions (RQ) with respect to the various factors of the KM infrastructure as well as with respect to the relationship between KM infrastructure and IC were raised and included into the research model: RQ 1 Do nonprofit organisations which have a Hierarchy culture have a stronger IT support than nonprofit organisations which have an Adhocracy culture? RQ 2 Do nonprofit organisations which have a centralised organisational structure have a stronger IT support than nonprofit organisations which have decentralised organisational structure? RQ 3 Do nonprofit organisations which have a stronger IT support have a higher value of Human Capital than nonprofit organisations which have a less strong IT support? RQ 4 Do nonprofit organisations which have a stronger IT support have a higher value of Structural Capital than nonprofit organisations which have a less strong IT support? RQ 5 Do nonprofit organisations which have a stronger IT support have a higher value of Relational Capital than nonprofit organisations which have a less strong IT support? In order to investigate the research questions, measurements for IC were developed which were linked to the main KM processes. The final KM/IC model contained four items for evaluating human capital, five items for evaluating structural capital and four items for evaluating relational capital. The research questions were investigated through empirical research using a case study approach with the focus on two nonprofit organisations providing trade promotions services through local offices worldwide. Data for the investigation of the assumptions were collected via qualitative as well as quantitative research methods. The qualitative study included interviews with representatives of the two participating organisations as well as in-depth document research. The purpose of the qualitative study was to investigate the factors of the KM infrastructure (organisational culture, organisational structure, IT support) of the organisations and how these factors were related to each other. On the other hand, the quantitative study was carried out through an online-survey amongst staff of the various local offices. The purpose of the quantitative study was to investigate which impact the level of IT support, as the main instrument of the KM infrastructure, had on IC. Overall several key themes were found as a result of the study: • Knowledge Management and Intellectual Capital were complementary with each other, which should be expressed through measurements of IC based on KM processes. • The various factors of the KM infrastructure (organisational culture, organisational structure and level of IT support) are interdependent. • IT was a primary instrument through which the different KM processes (creating, storing, sharing and applying knowledge) were performed. • A high level of IT support was evident when participants reported higher level of IC (human capital, structural capital and relational capital). The study supported previous research in the field of KM and replicated the findings from other case studies in this area. The study also contributed to theory by placing the KM research within the nonprofit context and analysing the linkage between KM and IC. From the managerial perspective, the findings gave clear indications that would allow interested parties, such as nonprofit managers or consultants to understand more about the implications of KM on OP and to use this knowledge for implementing efficient and effective KM strategies within their organisations.

Industry-specific human capital, knowledge labour, and industry wage structure in Taiwan

This paper investigates the role of industry-specific human capital (ISHC) in determining industry wage structure. The model presented in this paper distinguishes between knowledge labour and physical labour. Knowledge labour is physical labour embodied with ISHC. It is postulated that more ISHC-intensive industries, such as high-tech industries, pay higher wages and the wage premiums increase with workers' experience. The hypothesis is tested using a merged sample of 1997 - 1999 manpower utilization survey data from a newly industrialized economy - Taiwan. The findings show support for the effect of ISHC.

The BEE Undergraduate Model for Knowledge Development applied to the QUT Spatial Science Course : Diversity in Surveying Curriculum

This paper provides an overview of the current QUT Spatial Science undergraduate program based in Brisbane, Queensland, Australia. It discusses the development and implementation of a broad-based educational model for the faculty of built environment and engineering courses and specifically to the course structure of the new Bachelor of Urban Development (Spatial Science) study major. A brief historical background of surveying courses is discussed prior to the detailing of the three distinct and complementary learning themes of the new course structure with a graphical course matrix. Curriculum mapping of the spatial science major has been undertaken as the course approaches formal review in late 2010. Work-integrated learning opportunities have been embedded into the curriculum and a brief outline is presented. Some issues relevant to the tertiary surveying/ spatial sector are highlighted in the context of changing higher education environments in Australia.

Creativity, knowledge, engagement : keys to finding the right governance model for a regional community precinct

This paper investigates the Cooroy Mill community precinct (Sunshine Coast, Queensland), as a case study, seeking to understand the way local dynamics interplay and work with the community strengths to build a governance model of best fit. As we move to an age of ubiquitous computing and creative economies, the definition of public place and its governance take on new dimensions, which – while often utilizing models of the past – will need to acknowledge and change to the direction of the future. This paper considers a newly developed community precinct that has been built on three key principles: to foster creative expression with new media, to establish a knowledge economy in a regional area, and to subscribe to principles of community engagement. The study involved qualitative interviews with key stakeholders and a review of common practice models of governance along a spectrum from community control to state control. The paper concludes with a call for governance structures that are locally situated and tailored, inclusive, engaging, dynamic and flexible in order to build community capacity, encourage creativity, and build knowledge economies within emerging digital media cityscapes.

Towards a model for the analysis and description of mathematical knowledge and understanding

Mathematics education literature has called for an abandonment of ontological and epistemological ideologies that have often divided theory-based practice. Instead, a consilience of theories has been sought which would leverage the strengths of each learning theory and so positively impact upon contemporary educational practice. This research activity is based upon Popper’s notion of three knowledge worlds which differentiates the knowledge shared in a community from the personal knowledge of the individual, and Bereiter’s characterisation of understanding as the individual’s relationship to tool-like knowledge. Using these notions, a re-conceptualisation of knowledge and understanding and a subsequent re-consideration of learning theories are proposed as a way to address the challenge set by literature. Referred to as the alternative theoretical framework, the proposed theory accounts for the scaffolded transformation of each individual’s unique understanding, whilst acknowledging the existence of a body of domain knowledge shared amongst participants in a scientific community of practice. The alternative theoretical framework is embodied within an operational model that is accompanied by a visual nomenclature with which to describe consensually developed shared knowledge and personal understanding. This research activity has sought to iteratively evaluate this proposed theory through the practical application of the operational model and visual nomenclature to the domain of early-number counting, addition and subtraction. This domain of mathematical knowledge has been comprehensively analysed and described. Through this process, the viability of the proposed theory as a tool with which to discuss and thus improve the knowledge and understanding with the domain of mathematics has been validated. Putting of the proposed theory into practice has lead to the theory’s refinement and the subsequent achievement of a solid theoretical base for the future development of educational tools to support teaching and learning practice, including computer-mediated learning environments. Such future activity, using the proposed theory, will advance contemporary mathematics educational practice by bringing together the strengths of cognitivist, constructivist and post-constructivist learning theories.

Determining indicators of transportation disadvantage for establishing knowledge-based environments

Although, transportation disadvantage or imbalance between travel needs and supply of transportation system is a great harm to knowledge based environments, quantification and objectively measuring the state of transportation disadvantaged remain to be a great challenge for researcher due to its ambiguity. This poses questions of whether the current indicators are accurately linked with transportation disadvantages and the effectiveness of the current policies. Using current indicators of transportation disadvantages, the state of transportation disadvantage is often exaggerated due to limited afford has been put forward to provide clear assessment on the existed relationship between transportation disadvantage indicators with travel performance or capability. This paper proposes a structural equation model of transportation disadvantage using household variables gained from the 2006-2008 South-East Queensland Travel Survey (SEQTS). The underlying relationships between social economics and demographic characteristics of household with travel performance are modelled using a latent variable approach. The final model has been able to fit the data gathered from SEQTS and explained established links between key household factors with travel capability and determined key indicator of travel capability. The model recognises that travel capability is directly influenced by household factors; vehicle ratio, license possession, retirees and pensioners.