Development and application of in vivo expression technology (IVET) for analysing microbial gene expression in complex environments

Establishing the mechanisms by which microbes interact with their environment, including eukaryotic hosts, is a major challenge that is essential for the economic utilisation of microbes and their products. Techniques for determining global gene expression profiles of microbes, such as microarray analyses, are often hampered by methodological restraints, particularly the recovery of bacterial transcripts (RNA) from complex mixtures and rapid degradation of RNA. A pioneering technology that avoids this problem is In Vivo Expression Technology (IVET). IVET is a 'promoter-trapping' methodology that can be used to capture nearly all bacterial promoters (genes) upregulated during a microbe-environment interaction. IVET is especially useful because there is virtually no limit to the type of environment used (examples to date include soil, oomycete, a host plant or animal) to select for active microbial promoters. Furthermore, IVET provides a powerful method to identify genes that are often overlooked during genomic annotation, and has proven to be a flexible technology that can provide even more information than identification of gene expression profiles. A derivative of IVET, termed resolvase-IVET (RIVET), can be used to provide spatio-temporal information about environment-specific gene expression. More recently, niche-specific genes captured during an IVET screen have been exploited to identify the regulatory mechanisms controlling their expression. Overall, IVET and its various spin-offs have proven to be a valuable and robust set of tools for analysing microbial gene expression in complex environments and providing new targets for biotechnological development.

Testing temperature-induced proteomic changes in the plant-associated bacterium Pseudomonas fluorescens SBW25.

Traits used by bacteria to enhance ecological performance in natural environments are not well understood. Recognizing that the saprophytic plant-colonizing bacterium Pseudomonas fluorescens SBW25 experiences temperatures in its natural environment significantly cooler than the 28°C routinely used in the laboratory, we identified proteins differentially expressed between 28°C and the more environmentally relevant temperature of 14°C. Of 2102 protein isoforms, 32 were temperature responsive and identified by mass spectrometry. Seven of these (OmpR, MucD, GuaD, OsmY and three of unknown function, Tee1, Tee2 and Tee3) were selected for genetic and ecological analyses. In each instance, changes in protein expression with temperature were mirrored by parallel transcriptional changes. The fitness contribution of the genes encoding each of the seven proteins was larger at 14°C than 28°C and included two cases of trade-offs (enhanced fitness at one temperature and reduced fitness at the other – mucD and tee2 deletions). The relationship between the fitness effects of genes in vitro and in vivo was variable, but two temperature-responsive genes – osmY and mucD – contribute substantially to the ability of P. fluorescens to colonize the plant environment.

Structure and agency

The divide between agency and structural explanations of the causes of social phenomena has dominated research on housing as in other social fields. However, there has been some research that has sought to transcend this schism by combining agency and structural dimensions in the understanding of housing processes and outcomes. The article reviews the two most common approaches to doing this in housing research – structuration (following the work of Giddens) and critical realism. The example of research on homelessness is used to show how the approaches have been applied to housing issues.

Planning at the neighbourhood scale: localism, dialogic politics and the modulation of community action

This paper builds upon literature examining the foreclosing of community interventions to show how a resident-led anti-road-noise campaign in South-Eastern England has been framed, managed and modulated by authorities. We situate the case within wider debates considering dialogical politics. For advocates, this offers the potential for empowerment through non-traditional forums (Beck, 1994; Giddens, 1994). Others view such trends, most recently expressed as part of the localism agenda, with suspicion (Haughton et al, 2013; Mouffe, 2005). The paper brings together these literatures to analyse the points at which modulation occurs in the community planning process. We describe the types of counter-tactics residents deployed to deflect the modulation of their demands, and the events that led to the outcome. We find that community planning offers a space - albeit one that is tightly circumscribed - within which (select) groups can effect change. The paper argues that the detail of neighbourhood-scale actions warrant further attention, especially as governmental enthusiasm for dialogical modes of politics shows no sign of abating.

Chinese overseas doctoral student narratives of intercultural adaptation

This study aims to explore how Chinese overseas doctoral students adjust to a different academic, social and cultural environment, using Giddens’ theoretical framework of self-identity (1991). The findings indicate the participants proactively used various coping strategies in meeting challenges, and adapting to new social environments. Continuity and stability of self-identity were achieved either culturally or academically through self-reflexivity, autonomy, creativity, authenticity, and reliance on an ontological identity. The result is to challenge the grand narrative of essentialised “problematic Chinese learners”.

A structuration theory perspective on the institutional change in Higher Education sector

Budgeting system has been traditionally viewed as a control mechanism rather than a communication tool to facilitate the institutionalisation of organisational change. A good budgeting system not only reflects the organisational reality but also socially constructs the reality. This paper uses the structuration perspective to understand budget-related behaviour in a UK research-intensive university and especially, study the role of budgeting system in achieving organisational sustainability. Giddens’ structuration theory offers a valuable framework for the study of the duality of structure and emphasises on the structural properties of social systems. Based on the semi-structured interviews with top management and budget holders, it is concluded that in this specific context, budgeting system may place a significant role in establishing and legitimising institutional change.

Vi och de andra : En studie av hur män och kvinnor med invandrarbakgrund framställs i svenska invandrarfilmer från år 2000

Syftet med den här uppsatsen har varit att visa hur män och kvinnor med invandrarbakgrund framställs i svenska så kallade invandrarfilmer från år 2000. De frågeställningar jag arbetat utifrån är: Hur porträtteras män samt kvinnor med invandrarbakgrund i de svenska ”invandrarfilmerna” från år 2000? Vilka stereotyper kan man tänkas finna? Har det skett någon förändring i hur män och kvinnor med invandrarbakgrund porträtterats i svensk film? För att få svar på frågorna har jag genomfört textanalyser av fem filmer - Jalla! Jalla! av Josef Fares, Före stormen av Reza Parsa, Vingar av glas av Reza Bagher, Det nya landet av Geir Hansteen Jörgensen samt Bastarderna i paradiset av Luis R. Vera - utifrån teorier om genus, ”ras” och etnicitet. Teorierna har jag hämtat från bland andra Anthony Giddens, Yvonne Hirdman, Laura Mulvey, Paulina de los Reyes, Charles Ramírez Berg och Stuart Hall. Dessutom har jag jämfört filmerna med Rochelle Wrights studie av svenska filmer med invandrarkaraktärer från 1970-1990-talet för att se om det har skett någon förändring i gestaltningen av personer från andra delar av världen. Analyserna har visat att trots att filmerna från år 2000 är regisserade av personer som själva har invandrarbakgrund, så gestaltas invandrarna generellt sätt utifrån grova stereotyper. Det mest framträdande dragen i stereotyperna är att dessa, oavsett kön, gestaltas som avvikande samt som en del i en familjegrupp. Invandrarmän framställs som barnsliga, våldsbenägna, aggressiva samt som förtryckande gentemot kvinnor. Invandrarkvinnor framställs som konstiga, maktlösa, isolerade, okunniga offer samt som underordnad gentemot män. De filmer som delvis avviker, och som därmed uppvisar förändringar jämfört med tidigare filmer, är Före stormen och Det nya landet. I Före stormen har man nämligen kastat om könsrollerna. Invandrarmannen är underordnad gentemot kvinnorna, samtidigt som han gestaltas som en helt vanlig individ. I Det nya landet gestaltar man invandrarmän på ett mer positivt sätt. Fastän dessa gestaltas som grova stereotyper, revideras de på ett påtagligt sätt. De omarbetas genom att man tillskriver invandrare fördjupade egenskaper som är oförutsägbara under filmens gång. Vidare attribuerar man dem en logik som bidrar till att åskådarna nu får tillgång till att se på invandrarmän ur ett annat perspektiv, bortom den ensidiga stereotypbilden.

Att integrera i det svenska samhället : En undersökning om kvinnor med invandrarbakgrund och deras syn på introduktion och andra faktorer som påverkar inträdet i det svenska arbetslivet och samhället

The integration policy in Sweden shall encourage individuals to support themselves and take part in society. It shall alsocontribute to equal rights and opportunities for women as well as for men. In Borlänge this has resulted in a program ofintroduction for new arrivals from other countries. However, at the unit responsible for economic support, they havediscovered that women with immigrant background more often than men seem to have trouble starting or became to anending of the program, which then especially leads women to a long-term dependence for economic support. The purpose of this study has therefore been to investigate what factors affect immigrant women’s participation in theintroduction, and what significance this participation has for their possibilities to become economically self-supporting,and integrated into Swedish society. Previous research shows that some of the obstacles for the integration of immigrant women can be that they give birthto many children, are unskilled or have a low degree of education and that they tend to be living under patriarchalgender patterns. Another problem seem to be that some women are not even known as members of the municipalities.All of these problems are as well what was shown in my own study. I have used theoretical perspectives from Bourdieu, Elias & Scotson, Giddens, Roman and al-Baldawi in my analysis.Bourdieu have interesting thoughts about capital, habitus and field, which can help us to understand how individuals arebeing shaped and are given different opportunities to act in a special way or direction. Elias & Scotson describesthrough their study around established and outsiders how the process of integration can take place and what effects thatcan be shown for the opportunities to succeed in that part. Giddens, Roman and al-Baldawi then give us different waysto look at the patriarchy and family structures around the world. The result of this study shows that the willingness to integrate and be able to take care of your own support for living isan important part for the women for succeeding. For the other women, that don’t succeed, it turns out to be just like theprevious research has been shown. Gender patterns, many children and a low or no education skill all seems to be partof the issue. It is also suggested that the generosity of the Swedish welfare system might hinder rather than help someimmigrant women to become integrated into Swedish society.

Skolkuratorers upplevelser och erfarenheter av psykisk ohälsa hos dagens ungdomar : i ett föränderligt samhälle

Syftet med uppsatsen är att analysera vilka faktorer skolkuratorerna anser påverka ungdomars psykiska ohälsa. Vår ambitition är att visa på faktorer som kan tänkas ha påverkande betydelse för ungdomars psykiska ohälsa utifrån deras förändrande livsvillkor och möjligheter. Vi anser att deras position i skolan och den dagliga kontakt som de har med elever utgör en viktig del i att få ökad kunskap om problematiken ifråga. Följande frågeställningar kommer behandlas i uppsatsen.• Vilka är de centrala samhällsfaktorer som påverkar ungdomars psykiska ohälsa?• Vilka effekter har dessa på ungdomars livsvillkor och deras möjligheter att bli vuxna?Datainsamlingen har skett genom kvalitativa, strukturerade djupintervjuer med sex stycken skolkuratorer på fem gymnasieskolor i en större och en mindre stad i mellan Sverige. Alla kuratorer är kvinnor och är i åldrarna mellan tjugosju till sextiofem år. De teorier som ligger till grund för studien är Anthony Giddens modernitets teori samt teorier om samband mellan risk-, frisk och skyddsfaktorer.De slutsatser som kan dras utifrån denna uppsatsstudie är att respondenterna upplever att det finns centrala faktorer som påverkar den psykiska ohälsan hos ungdomar. Faktorer som bland annat hemförhållande, föräldrars psykiska mående och ekonomiska förhållanden samt de ungas bristande kontakt med vuxenvärlden. Resultatet visar även att samhällsförändringar är en påverkande faktor på ungas psykiska hälsa. Det handlar främst om att vi idag har ett ökat informationsflöde där media och internet, enligt våra respondenter är en viktig faktor till att unga mår allt sämre idag. Vi har även kunnat se utifrån vårt resultat att dagens moderna samhälle bidragit till att det har skett en utveckling från ett traditionellt kollektivt samhälle till ett mer individualiserat och differentierat samhälle där den enskilda individen står allt mer i fokus. Den tidigare forskning vi hänvisar till visar på likheter med det resultat vi fått fram.

Narration, Visualization and Mind : Movies in everyday life as a resource for utopian self-reflection

The paper is analyzing how people in late modern society characterized by de-traditionalization, use moving images as a cultural resource for the construction of meaningful subjective world views. As a theoretical concept with several dimensions, “sacralization of the self” (Woodhead & Heelas 2000: 344), is related to media theory. With a critical focus on ‘the self’, as a core aspect in contemporary media society Eric W. Rothenbuhler labels the individual self as one of “the sacred objects of modern culture” (Rothenbuhler 2006: 31).I want to emphasize the need for case studies in order to undertake a critical investigation about ‘the self’ and how consumption of fiction film is interconnected to spectator´s creation of self images, but also to understand how film engagement elicits self-reflection (Giddens 1991, Axelson 2008, Vaage 2009a). The paper make use of empirical data to illustrate and theoretically develop perspectives on how the audience uses fiction film in every-day life for the construction of the self, as well for the construction of more profound and long-lasting ideas of being part of a moral community (Brereton 2005, Jerslev 2006, Klinger 2008, Mikkola et al. 2007, Vaage 2009b). Some empirical findings support a conclusion that moving images creates a transitional space for the human mind, with the capacity of transporting the spectator from real life to fiction and back to real life again, helping the individual with an ongoing process of transforming the self, dealing with who you actually are, and who you want to become (Axelson 2008, Vaage 2009b).

Konsekvenser av rollövertagandet : ”I”, ”me” och individualiteten

Consequences of role taking: the ”I”, the ”me” and individuality This paper analyzes the tendency of taking the ”I” and the ”me” in G. H. Meads theory of role taking as two separate, qualitatively different parts of human personality, the ”I” being of individual origin, the ”me” of social. In the original Meadian sense role taking gives rise to both the ”I” and the ”me”. They are dialectically united rather than dualistically separated aspects. They refer to the joint functional power of the subject, finding itself as an object, mediated by the Other. As a consequence, the link between body and the social world becomes theoretically more stringent, as the body is given its place as a cognitive social object among others, this by contrast to interpretations where the body is left as an object mainly outside the human social experience and as a source of agency sui generis, a conception which is in opposition to Meads. The stress on the ”I”-phase, as related to body and concrete action in combination with its direct relation to the ”me”-phase, actualizes Mead as a forerunner in modern biologic/neurologic research on human perceptual, motivational and intentional capacity.Swedish Mead interpreters are critically analyzed. Interpretations of Charon, Giddens, Joas and Habermas are partly scrutinized. The author defines the conceptual pair in terms of activity, subjectivity, temporal relativity and distance.

Motståndets betydelse : Ett bidrag till aktör-strukturdebatten

The significance of inhibition: a contribution to the agency-structure debate A central problem in social theory today is how to integrate agency and structure. The vital question is how to explain social reality by proceeding from both the notion of people doing things which affect the social relationships in which they are embedded (agency) and the idea of the social context moulding social activity (structure). Sociologists as Pierre Bourdieu, Anthony Giddens and Jürgen Habermas call attention to social practices as the ”missing link” between agency and structure. In accentuating social practices, the aim is to explain how people in their daily encounters actively contribute to the production and reproduction of social structures. This article puts forth the posthumous contribution of George Herbert Mead to the agency-structure debate. I argue that his social pragmatist theory gives us a compound and thorough– but not fully recognized – explanation of the dynamics and the course of events in structurally framed encounters. By especially emphasizing the importance Mead ascribes to the inhibited social act, I examine how his theory deepens the understanding of social practices as a bridge between agency and structure.

Självet, rollerna och pågående koordinationer av mänskliga handlingar : Om att försöka se ’samhället’ som något annat än fängelse eller marionetteater

The self, roles and the ongoing coordination of human action. Trying to see ‘society’ as neither prison nor puppet theatre In the article it is argued that structural North-American role-sociology may be integrated with theories emphasizing ‘society’ as ongoing processes (f. ex. Giddens’ theory of structuration). This is possible if the concept of role is defined as a recurrence oriented to the action of others standing out as a regularity in a societal process. But this definition makes it necessary to in a fundamental way understand what kind of social being the role-actor is. This is done with the help of Hans Joas’ theory of creativity and Merleau-Pontys concept of ‘flesh’ arguing that Meads concept of the ‘I’ maybe understood as an embodied self-asserting I, which at least in reflexive modernity has the creative power to split Meads ‘me’ into a self-voiced subject-me and an other voiced object-me. The embodied I communicating with the subject-me may be viewed as that role-actor which is something else than the role played. But this kind of role-actor is making for new troubles because it is hard to understand how this kind of self is creating self-coherence by using Meads concept of ‘the generalized other’. This trouble is handled by using Alain Touraines concept of the ‘subject’ and arguing that the generalized other is dissolving in de-modernized modernity. In split modernity self-coherence may instead be created by what in the article is called the generalized subject. This concept means a kind of communicative future based evaluation, which has its base in the ‘subject’ opposing the split powers of both the instrumentality of markets and of life-worlds trying to create ‘fundamentalistic’ self-identities. This kind of self is communicative because it also must respect the other as ‘subject’. It exists only in the battle against the forces of the market or a community. It never constructs an ideal city or a higher type of individual. It creates and protects a clearing that is constantly being invaded, to use the words of the old Frenchman himself. Asa kind of test-case it is by the way in the article shown how Becks concept of individualization may be understood in a deeply social and role-sociological way.

Working out intimacy: young people and friendship in an age of reflexivity

Drawing upon a longitudinal, interview-based study of Australian secondary school students, this article explores young people's friendship experiences and attitudes to intimacy and the interpersonal. The discussion develops in relation to the work of Anthony Giddens on detraditionalisation and reflexivity, and Nikolas Rose on modernity and the self. First, I argue that feminism and psychotherapeutic ways of constituting and knowing the self are reconfiguring the cultural meanings of intimacy. Second, I suggest that this reworking of intimacy has differential and uneven effects and has particular consequences for the production of gendered subjectivities. Third, I raise some critical questions about the extent to which either Giddens's or Rose's account can properly capture the gendered and situated experiences of intimacy. I offer examples in which gender is being rearticulated in new yet familiar ways and note some persistent tensions in desires for connection and community versus autonomy and freedom. Carol Gilligan's work on gender differences in orientations to autonomy and connection is briefly revisited. Overall, it is argued that we need to take more account of how class, location and schooling differences influence dispositions to friendship and the interpersonal, and this is elaborated through a discussion of the 'relationship orientations' of two white Australian young men.

Globalisation and reflexivity: some challenges for research into quality education mediated by ICTs

Globalisation can be characterised, Giddens (1994) suggests, as a process of 'intensified reflexivity' that creates the conditions for 'a world of clever people'. Information and Communication Technologies (ICTs) are fundamental to globalisation and they have also been incorporated into the new educational technologies deployed by educators to (re)create 'a world of clever people'. Together, education and the ICTs are strong forces for globalisation where both curriculum and pedagogy shape the knowledge and values of the rising generation of 'clever people'. This chapter posits some research issues and questions that might be usefully pursued in transnational collaborative research or are germane to its conduct and contexts. These matters include: the place of ICTs in research work; the challenge of globally inclusive curricula and the impact of English as the global language; and ICTs decentring the research centre.