Genetic Dissection of Endocytic Trafficking in Drosophila Using a Horseradish Peroxidase-Bride of Sevenless Chimera: hook Is Required for Normal Maturation of Multivesicular Endosomes

Mutations in the hook gene alter intracellular trafficking of internalized ligands in Drosophila. To dissect this defect in more detail, we developed a new approach to visualize the pathway taken by the Bride of Sevenless (Boss) ligand after its internalization into R7 cells. A chimeric protein consisting of HRP fused to Boss (HRP-Boss) was expressed in R8 cells. This chimera was fully functional: it rescued the boss mutant phenotype, and its trafficking was indistinguishable from that of the wild-type Boss protein. The HRP activity of the chimera was used to follow HRP-Boss trafficking on the ultrastructural level through early and late endosomes in R7 cells. In both wild-type and hook mutant eye disks, HRP-Boss was internalized into R7 cells. In wild-type tissue, Boss accumulated in mature multivesicular bodies (MVBs) within R7 cells; such accumulation was not observed in hook eye disks, however. Quantitative electron microscopy revealed a loss of mature MVBs in hook mutant tissue compared with wild type, whereas more than twice as many multilammelar late endosomes were detected. Our genetic analysis indicates that Hook is required late in endocytic trafficking to negatively regulate delivery from mature MVBs to multilammelar late endosomes and lysosomes.

New York, N.Y. and vicinity, 1961 : Sandy Hook, N.J.-N.Y. (8 Sheet Set) (Raster Image)

This layer is a georeferenced raster image of the United States Geological Survey 7.5 minute topographic sheet map entitled: New York and vicinity : Sandy Hook, N.J.-N.Y., 1954. It is part of an 8 sheet map set covering the metropolitan New York City area. It was published in 1961. Scale 1:24,000. The source map was prepared by the Geological Survey from 1:24,000-scale maps of Sandy Hook, Keyport, Marlboro, and Long Branch 1954 7.5 minute quadrangles compiled by the Army Map Service. Culture revised by the Geological Survey. Hydrography compiled from USC&GS charts 286, 369, and 824. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM) Zone 18N NAD27 projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. USGS maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works of humans, such as roads, railroads, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 10 and 20 feet; depths are shown with contours and soundings. Please pay close attention to map collar information on projections, spheroid, sources, dates, and keys to grid numbering and other numbers which appear inside the neatline. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Savery-Pot Hook project (CO,WY) : environmental impact statement.

Mode of access: Internet.

Ploughshare and pruning-hook; ten lectures on social subjects,

Great possessions. - Crime and punishment. - Christianity a danger to the state. - The salt of the earth. - The rights of majorities. - Discreditable conduct. - What is womanly? - Use and ornament. - Art and Citizenship. - Conscious and unconscious immortality.

The human touch in business; the story of Charles R. Hook, who rose from office boy to internationally-known business leader.

Mode of access: Internet.

Evaluation of catch-and-release, hook-and-line vulnerability and heredity of vulnerability of a largemouth bass population : final report /

"Federal aid project F-27-R, U.S. Fish and Wildlife Service."

Direct and general support and depot maintenance repair parts and special tools lists : mixer, rotary tiller, diesel engine driven, 4 wheel, pneumatic tires, self-propelled, with flat hook tines (Rex Chainbelt, Inc. model HDTM), FSN 3895-987-5536).

"April 1969."

Organizational maintenance repair parts and special tools lists : mixer, rotary tiller, diesel engine driven, 4 wheel, pneumatic tires, self-propelled, with flat hook tines (Rex Chainbelt, Inc. model HDTM), FSN 3895-987-5536).

"10 February 1970"--Change no. 1.

Proceedings of the general court martial convened for the trial of Commodore James Barron, Captain Charles Gordon, Mr. William Hook, and Captain John Hall, of the United States' ship Chesapeake, in the month of January, 1808 /

Mode of access: Internet.