[¿Es necesaria la influencia de la luz para la vida?] : discurso pronunciado en el salon de actos de la Universidad Central /

Tesis Universidad Central, leída el 23 de diciembre de 1855.

Fotodegradação de pesticidas: caso do fenarimol

Tese dout. em Química, Unidade de Ciências Exactas e Humanas, Univ. do Algarve, 1997

New indolium fluorofores for two-photon absortion (2PA) bioimaging applications

The development of organic materials with 2PA has attracted intensive attention in the past two decades [1]. In two-photon bio-imaging applications the design of the chromophore requires to have a good cross-section (σ2PA) and good biological compatibility which depends on the molecular volume and polarity [2]. In this work, we present the design, synthesis and characterization of new indolium derivatives. These compounds are easy to achieve with good yields and good photophysical properties. In addition, time-dependent density functional theory (TDDFT) has been carried out to investigate the energy level of the ground and excited state. Their spectral properties and assays performed on cultured cells, demonstrate the potential of these compounds as fluorescent probes with application in two-photon bio-imaging.

New bodipy dyads for two-photons fluorescence imaging in cells

Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, due to their advantageous features in tissue imaging compared to the corresponding one-photon probes [1]. Recently, we have designed and synthetized an aminonaphthalimide–BODIPY derivative as energy transfer cassettes and were found to show very fast and efficient BODIPY fluorescence sensitization [2]. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In order to increase the two-photon absorption of the system aminonaphthalimide fluorophore was replace with a Prodan analog (BODIPY dyad 1), which presents found a variety of applications as probes and labels in biology [3]. The two-photon absorption cross-section  of the dyads is significantly incremented by the presence of the 6-acetyl-2-naphthylamine donor group. The emission maximum of a BODIPY fluorophore can significantly be red-shifted in comparison to their precursors by conjugation with aromatic aldehydes. [4] We use a synthetic strategy to obtain BODIPY dyad 2 that incorporates an imidazole ring. This molecule can be used in biological media as a near-neutral pH indicator based on one- and two-photon excitable BODIPY acceptor.

Modeling and analysis of thermoelectric energy conversion efficiency in nanostructures.

116 p.

El rol del alumno en clase: de la observación a la acción

Con el propósito de aumentar el aprendizaje activo del alumno durante las clases se han introducido distintas mejoras en la metodología docente y en el sistema de evaluación de la asignatura Fundamentos Matemáticos de la Ingeniería II del primer curso del Grado en Ingeniería Civil de la Universidad de Alicante durante el curso 2014-2015. Su objetivo es reducir el tiempo dedicado a la clase magistral, en la que el alumno tiene un rol fundamentalmente observador, y aumentar la participación activa del alumno. Esto se ha conseguido incentivando la resolución autónoma de diversos ejercicios y problemas por parte de los alumnos por dos vías: (1) cambiando la dinámica de las clases prácticas, y (2) aumentando el número de exámenes en la evaluación continua. En el primer caso se cuenta con la supervisión del profesor, mientras que en la segunda se obtiene una realimentación del trabajo realizado. El resultado de esta experiencia es dispar. Por un lado, los alumnos no han encontrado una mejora en la nueva dinámica de clase, pero sí han aceptado muy favorablemente el aumento en el número de exámenes, hasta el punto de que al 80% le gustaría que otras asignaturas adaptaran este proceso de evaluación.