In vitro microbial inoculum: A review of its function and properties

This review considers microbial inocula used in in vitro systems from the perspective of their ability to degrade or ferment a particular substrate, rather than the microbial species that it contains. By necessity, this required an examination of bacterial, protozoal and fungal populations of the rumen and hindgut with respect to factors influencing their activity. The potential to manipulate these populations through diet or sampling time are examined, as is inoculum preparation and level. The main alternatives to fresh rumen fluid (i.e., caecal digesta or faeces) are discussed with respect to end-point degradabilities and fermentation dynamics. Although the potential to use rumen contents obtained from donor animals at slaughter offers possibilities, the requirement to store it and its subsequent loss of activity are limitations. Statistical modelling of data, although still requiring a deal of developmental work, may offer an alternative approach. Finally, with respect to the range of in vitro methodologies and equipment employed, it is suggested that a degree of uniformity could be obtained through generation of a set of guidelines relating to the host animal, sampling technique and inoculum preparation. It was considered unlikely that any particular system would be accepted as the 'standard' procedure. However, before any protocol can be adopted, additional data are required (e.g., a method to assess inoculum 'quality' with respect to its fermentative and/or degradative activity), preparation/inoculation techniques need to be refined and a methodology to store inocula without loss of efficacy developed. (c) 2005 Elsevier B.V. All rights reserved.

A review and simplification of the in vitro incubation medium

The requirement to rapidly and efficiently evaluate ruminant feedstuffs places increased emphasis on in vitro systems. However, despite the developmental work undertaken and widespread application of such techniques, little attention has been paid to the incubation medium. Considerable research using in vitro systems is conducted in resource-poor developing countries that often have difficulties associated with technical expertise, sourcing chemicals and/or funding to cover analytical and equipment costs. Such limitations have, to date, restricted vital feed evaluation programmes in these regions. This paper examines the function and relevance of the buffer, nutrient, and reducing solution components within current in vitro media, with the aim of identifying where simplification can be achieved. The review, supported by experimental work, identified no requirement to change the carbonate or phosphate salts, which comprise the main buffer components. The inclusion of microminerals provided few additional nutrients over that already supplied by the rumen fluid and substrate, and so may be omitted. Nitrogen associated with the inoculum was insufficient to support degradation and a level of 25 mg N/g substrate is recommended. A sulphur inclusion level of 4-5 mg S/g substrate is proposed, with S levels lowered through omission of sodium sulphide and replacement of magnesium sulphate with magnesium chloride. It was confirmed that a highly reduced medium was not required, provided that anaerobic conditions were rapidly established. This allows sodium sulphide, part of the reducing solution, to be omitted. Further, as gassing with CO2 directly influences the quantity of gas released, it is recommended that minimum CO, levels be used and that gas flow and duration, together with the volume of medium treated, are detailed in experimental procedures. It is considered that these simplifications will improve safety and reduce costs and problems associated with sourcing components, while maintaining analytical precision. (c) 2005 Elsevier B.V. All rights reserved.

Adjustments in the forcing-feedback framework for understanding climate change

The traditional forcing-feedback framework has provided an indispensable basis for discussing global climate changes. However, as analysis of model behavior has become more detailed, shortcomings and ambiguities in the framework have become more evident and physical effects unaccounted for by the traditional framework have become interesting. In particular, the new concept of adjustments, which are responses to forcings that are not mediated by the global mean temperature, has emerged. This concept, related to the older ones of climate efficacy and stratospheric adjustment, is a more physical way of capturing unique responses to specific forcings. We present a pedagogical review of the adjustment concept, why it is important, and how it can be used. The concept is particularly useful for aerosols, where it helps to organize what has become a complex array of forcing mechanisms. It also helps clarify issues around cloud and hydrological response, transient vs. equilibrium climate change, and geoengineering.

Multi-Objective Pipe Smoothing Genetic Algorithm For Water Distribution Network Design

This paper describes the formulation of a Multi-objective Pipe Smoothing Genetic Algorithm (MOPSGA) and its application to the least cost water distribution network design problem. Evolutionary Algorithms have been widely utilised for the optimisation of both theoretical and real-world non-linear optimisation problems, including water system design and maintenance problems. In this work we present a pipe smoothing based approach to the creation and mutation of chromosomes which utilises engineering expertise with the view to increasing the performance of the algorithm whilst promoting engineering feasibility within the population of solutions. MOPSGA is based upon the standard Non-dominated Sorting Genetic Algorithm-II (NSGA-II) and incorporates a modified population initialiser and mutation operator which directly targets elements of a network with the aim to increase network smoothness (in terms of progression from one diameter to the next) using network element awareness and an elementary heuristic. The pipe smoothing heuristic used in this algorithm is based upon a fundamental principle employed by water system engineers when designing water distribution pipe networks where the diameter of any pipe is never greater than the sum of the diameters of the pipes directly upstream resulting in the transition from large to small diameters from source to the extremities of the network. MOPSGA is assessed on a number of water distribution network benchmarks from the literature including some real-world based, large scale systems. The performance of MOPSGA is directly compared to that of NSGA-II with regard to solution quality, engineering feasibility (network smoothness) and computational efficiency. MOPSGA is shown to promote both engineering and hydraulic feasibility whilst attaining good infrastructure costs compared to NSGA-II.

Growth and exchange rate volatility: a panel data analysis

The aim of this article is to assess the role of real effective exchange rate volatility on long-run economic growth for a set of 82 advanced and emerging economies using a panel data set ranging from 1970 to 2009. With an accurate measure for exchange rate volatility, the results for the two-step system GMM panel growth models show that a more (less) volatile RER has significant negative (positive) impact on economic growth and the results are robust for different model specifications. In addition to that, exchange rate stability seems to be more important to foster long-run economic growth than exchange rate misalignment

O memorial autobiográfico: uma tradição acadêmica do ensino superior no Brasil

The thesis has as object of study the autobiographical memmorials. The general objective is to describe the history of the memmorial as an academic tradition of higher education in Brazil. Considered a hybrid genre, memmorials are known for focusing on life stories from a scientific perspective. The investigation revolves around three intertwined branches: History of Education, educational practices and language usages, which allow us to conduct a dialogue with multiple theoretical-methodological references with a view to supporting our analyses. The corpus used for the analysis was made up of 40 autobiographical memmorials, distributed as follows: 16 academic memmorials, dated from 1935 to 1970; 07 academic memmorials, dated from 1980 to 2007; and 17 formation memmorials, dated from 1995 to 2000. In this corpus, we also included official documents, which relate to legislation contained in edicts, resolutions, ordinances, regulations, which we used with a view to: 1) getting to know and understanding the big picture of higher education regulation in Brazil and the aspects related to the higher education teaching career; 2) investigating the text of memmorials in the light of the injunctive discourse characteristic of the edicts and resolutions in which they were based. The analysis of the memmorial supported by the legislation which regulates it allowed us to reconstitute the image of the professor throughout 80 years in the Brazilian public university. For this purpose, the study was conducted in the theoretical-methodological perspective of the (auto)biographical research in Education and of the sociolinguistic studies on discourse genres and discursive traditions. The investigations reveal the memmorial as an academic genre in which the professor's academic-professional history and the history of the higher education teaching career in Brazil intertwine. Anchored in the Bakhtinian perspective on discourse genres, according to which the memmorials evolve and become more complex as their contexts of usage also evolve and become more complex themselves, the results of our analyses allowed us to correlate genre changes to the sociohistorical context and to its usage as an educational practice in the university, in the decades under study. Therefore, the analyses showed that these self-writings: go from latent subjectivity to pure objectivity from the 1930s to 1960s; they show total annulment of the subject from the 1960s to the 1970s; they reappear in the 1980s, having Professor Magda Soares' memmorial as perspective; they expand and diversify from the 1990s onwards, taking on a formative role and a perspective of future as well. So far as language usages are concerned, we investigated the relationship of the subject with the language, especifically the manifestation of alterity on the discursive tissue of the memmorials. In this branch, the analyses pointed to the influence of the authoritative discourse on the formation of the professor and of the injunction and reinventing discourses on the authorship process. Therefore, the autobiographical memmorial reveals itself as a specific expression of the Brazilian academy's cultural sphere and allows us to confirm the hypothesis that each memorial tackles a singular-plural situation, by presenting a dialectical articulation between private and public, according to the institutional structures, in which and with which the professor has already formed him/herself and with which he/she dialogues

Molar Pregnancy in Adolescents

OBJECTIVE: To describe the clinical presentation of hydatidiform molar pregnancy in women under the age of 20 years. In addition, we sought to understand if this adolescent population manifests differences in clinical factors compared to an adult population that may affect outcome.STUDY DESIGN: We used a database from the New England Trophoblastic Disease Center to analyze clinical data from all women followed for molar pregnancy between 1970 and 2009 with complete follow-up information. This population was stratified by age and clinical parameters including presenting signs, molar histology and development of gestational trophoblastic neoplasia (GTN). Univariable and multivariable logistic regression was employed to discern clinical factors that associated with adolescent age. The Partners Human Research Committee approved this study.RESULTS: We identified 1,494 women diagnosed with hydatidiform mole (HM), of which 220 (14.7%) were adolescents defined as age <20 years. The most common presenting clinical signs were vaginal bleeding and an enlarged uterus compared to dates. Median gestational age at diagnosis was 13.4 weeks, not different from that in the adult population. Similarly, no difference in presenting human chorionic gonadotropin was observed between the adult and adolescent populations. Adolescents presented with a significant overrepresentation of complete mole (86% vs. 75%, p < 0.001) compared to adults. Complete mole was associated with a heightened risk of developing GTN (OR 2.6, 95% CI 1.9-3.5), and despite the association of complete mole with young maternal age, univariable analysis showed no difference in the rate of GTN observed between adolescents and adults (24% vs. 30%, p = 0.08). Multivariable analysis controlling for molar histology demonstrated that adolescent age was associated with a decreased risk of GTN (hazard ratio 0.67, 95% CI 0.48 0.93).CONCLUSION: Adolescents account for a substantial proportion of the population with HM. They commonly present with vaginal bleeding. Though this population develops a complete mole with a higher frequency than adults, adolescents appear to have a significantly decreased risk of developing GTN. (J Reprod Med 2012; 57:225-230)

Decentralization and reform in Latin America: improving intergovernmental relations

Includes bibliography

Fatal aortic endocarditis associated with community-acquired Serratia marcescens infection in a dog

A 12 yr old Dalmatian was referred for evaluation of acute lethargy, fever, neurologic signs, and a recently ausculted heart murmur. Echocardiography in combination with blood cultures resulted in a diagnosis of nonhospital-acquired Serratia marcescens bacteremia and aortic valve endocarditis. Despite early diagnosis and aggressive therapy, the dog failed to respond to antimicrobials and died within 6 hr after admission. Necropsy findings included aortic valve endocarditis, septicemia, and diffuse thromboembolic disease. There was no history of pre-existing underlying disease or immunosuppressive therapy, and the dog had not been hospitalized before referral.

Unilateral epididymitis associated with salmonella bacteremia in a dog

This report describes the clinical features, diagnosis and treatment of a dog with unilateral epididymitis associated with Salmonella spp. bacteremia. Fever and an enlarged and painful testicle were the main clinical signs that resulted in referral for diagnostic evaluation. Unilateral septic epididymitis was diagnosed via ultrasonography of the genitourinary tract and aerobic culture of scrotal fluid, urine and blood, which yielded heavy growth of Salmonella spp. Pulsed-field gel electrophoresis (PFGE) confirmed the presence of Salmonella javiana. Following antibiotic therapy there was total resolution of clinical signs, and no Salmonella was isolated from a post-treatment urine culture. The source of infection was unknown, however an environmental exposure was suspected. Although infrequent, infection with Salmonella spp. should be included in the differential diagnosis of canine epididymitis. Given the major zoonotic importance of salmonellosis, and to prevent re-infection after treatment, the source of the infection should be investigated and eliminated, if possible.

El pensamiento creativo de Fernando Higueras

Para acercarnos al pensamiento creativo de Fernando Higueras como arquitecto, partimos del estudio de sus constantes creativas en evolución cronológica divididas en 9 direcciones de investigación. En ellas observamos la importancia de su obra NO CONSTRUIDA, como germen de las constantes creativas de su pensamiento y parte de ella la estudiamos, analizamos y reconstruimos gráficamente en 3D. Fernando Higueras aprendía de sus obras o propuestas arquitectónicas anteriores llegando a la madurez constructiva y formal colmada de Belleza a través de la composición y combinación de dichas constantes con pasmosa naturalidad. Además es importante añadir, para llegar a la comprensión de las constantes del pensamiento creativo del arquitecto, el componente de “intuición” de todo artista, de su modo de mirar y observar las cosas como fuente de inspiración. El “modo de mirar” del arquitecto nos habla de su sensibilidad al hábitat popular y vernáculo del hombre. Gracias a Antonio Miró en el período 1963-1970, Fernando Higueras pudo dar marco real a gran parte de sus ideas constructivas. El objeto de estudio del presente trabajo es llegar a las “invariantes” del arquitecto para comprender su pensamiento creativo. Los objetivos del presente estudio han sido conocer mejor su pensamiento, su obra desde un punto de vista cronológico-evolutivo para poder llegar a entender las relaciones y conexiones creativas entre unas y otras, observar y constatar la evolución de sus constantes creativas y compositivas, de analizar gráficamente parte de su obra no construida para llegar a la mejor comprensión de dichas constantes reconstruyéndolas con los medios gráficos digitales actuales. La reconstrucción en 3D de parte de su obra no construida constata la magnitud de su pensamiento creativo. La metodología empleada parte de la recopilación de toda la información necesaria, escritos, artículos en revistas o periódicos, críticas, observaciones, planos, proyectos, memorias, fotografías de la época, maquetas, opiniones de sus contemporáneos, familiares o amigos y visita de parte de su obra construida. A partir de ahí se fijan 9 direcciones de investigación para cada constante de su pensamiento creativo. En cada dirección se analiza desde dos puntos de vista diferentes. Un primer punto de vista te��rico o cronológico-evolutivo a partir de la selección de algunas de sus obras construidas y no construidas. Y otro segundo punto de vista gráfico basándose en el estudio y reconstrucción de parte de su obra no construida. En resultado, se ha estudiado la evolución de su obra viendo las conexiones y relaciones entre unas y otras llegando a la madurez de sus constantes creativas en sus obras, algunas construidas y otras no construidas. Fernando Higueras se perfecciona así mismo, intentando hacer las cosas cada vez un poco mejor, llegando a dominar sus propias constantes en total madurez y expresión formal y constructiva. La elaboración de modelos tridimensionales en 13 proyectos de su obra no construida nos refresca la capacidad de su pensamiento creativo, además de analizar en parte de ellas los gérmenes constructivos y compositivos de muchas de sus ideas o constantes creativas. Finalmente, este trabajo ha contribuido a llegar a entender a Fernando Higueras en una dimensión m��s amplia, desde el frescor de su obra no construida hasta la capacidad de su pensamiento creativo, de la evolución de sus constantes, del estudio de sus últimos proyectos no construidos, de su sensibilidad a todas las artes y a todo lo que engloba Belleza. Las 360 láminas de dibujos que se aportan inician el estudio y análisis de su vasta obra no construida. ABSTRACT The analysis of Fernando Higueras's creative thought as an architect begins with a study of his main creative themes and their chronological evolution, divided into nine directions of research. Here we see the importance of his UNBUILT work as the germ of the creative themes of his thought, part of which we study, analyse and reconstruct graphically in 3-D. Fernando Higueras drew on his earlier architectural works and ideas and reached an ultimate constructive and formal maturity that was infused with beauty through composition and the amazingly natural combination of these keynote themes. In order to gain a greater understanding of the key themes of the architect's creative thought it is also worth noting the element of artists' intuition, their way of seeing and observing the world as a source of inspiration. The architect's "way of seeing" reveals his sensitivity to the popular and traditional human habitat. Thanks to Antonio Miró, in the period between 1963 and 1970 Fernando Higueras was able to provide a real framework for a large part of his constructive ideas. The object of study of the present work is to determine the architect's “invariables” as a means of understanding his creative thought. The aims of the present study are to explore his thoughts in greater depth, to examine his work from the chronological-evolutionary point of view for an insight into the relationships and the creative connections between them, to observe and apprehend the evolution of his main creative themes and compositional keynotes, to analyse graphically part of his unbuilt work in order to improve our understanding of these underlying themes through their reconstruction using the latest graphic digital resources. The 3-D reconstruction of part of his unbuilt work highlights the magnitude of his creative thought. The methodology used to collect all the necessary information includes an examination of his writings and articles in journals and newspapers, criticisms, observations, plans, projects, building specifications, period photographs, models, the opinions of his contemporaries, family and friends, and visits to some of his built work. Nine directions of research were then established for each main theme of his creative thought. Each direction includes an analysis from two different points of view: the first, theoretical or chronological-evolutionary aspect, based on a selection of some of his built and unbuilt works; and a second graphic aspect based on the study and reconstruction of part of his unbuilt work. The result is a survey of the evolution of his work with a view to establishing the connections and their interrelationships, through to the ultimate maturity of his creative themes in both his built and unbuilt work. Fernando Higueras is on a constant quest for excellence, a perpetual desire to do things a little better, until a point where he gains mastery over his own recurrent themes, in the fullness of maturity and of his formal and constructive expression. The creation of three-dimensional models in 13 projects of his unbuilt work offers a new look at the capacity of his creative thought, in addition to the analysis of the evidence in some of these projects of the constructive and compositional germs of many of his ideas and themes. Finally, this work has contributed to the understanding of Fernando Higueras in a broader dimension, from the freshness of his unbuilt work through to the capacity of his creative thought, the evolution of his main themes, the study of his final unbuilt works, his sensitivity to the arts as a whole and to everything beautiful. The 360 plates of illustrations provided form the basis of the study and analysis of his extensive unbuilt work.

A PESQUISA ACADÊMICA SOBRE COMUNICAÇÃO POPULAR, ALTERNATIVA E COMUNITÁRIA NO BRASIL: análise de dissertações e teses produzidas em Programas de Pós-Graduação em Comunicação entre 1972 - 2012

Mapeamento das dissertações e teses referentes à subárea da comunicação popular, alternativa e comunitária (CPAC) desenvolvidas nos Programas de Pós-Graduação em Comunicação stricto sensu no Brasil, de 1972 a 2012. Dentre os objetivos estão localizar as pesquisas; os autores; sua distribuição no tempo e espaço; identificar as instituições e orientadores que impulsionam a subárea; definir as abordagens te��rico-metodológicas; e apontar autores/conceitos referência. Por meio de pesquisa exploratória e aplicação de quatro filtros, chegou-se a uma amostra final de 102 pesquisas, 87 dissertações e 15 teses, submetidas à análise quantitativa, por meio de Análise de Conte��do a partir de partes pré-definidas (Resumo, Palavras chave, Introdução, Sum��rio, Considerações Finais e capítulo metodológico, quando presente), e a uma análise qualitativa do conte��do completo das 15 teses. O m��todo que orienta esta pesquisa é o histórico dialético, na perspectiva da busca de uma análise de conjunto e atenta às contradições e mudanças que o objeto está implicado; e a pesquisa bibliográfica que a fundamenta se ancora em autores como Jorge González, Cicilia Peruzzo, Regina Festa, Pedro Gilberto Gomes, Gilberto Gim��nez e Augusto Triviños e foi realizada com o apoio do software NVivo. Resultados quantitativos indicam: a) predominância de pesquisas sobre comunicação comunitária (68%) b) predominância de estudos empíricos (79%); c) a variedade de denominações atribuídas às experiências pelos pesquisadores; d) a constante luta das classes populares por democratização da comunicação e por direitos sociais ao longo dos anos; e) a influência e importância dos intelectuais orgânicos nas experiências estudadas, f) problemas metodológicos; g) UMESP, USP e UFRJ como instituições protagonistas, e, h) Cicilia Peruzzo e Raquel Paiva como as que mais orientam teses e dissertações sobre a temática. Quanto à análise qualitativa verificaram-se alguns critérios que permeiam a CPAC: 1) a definição de classes subalternas; 2) a importância da participação ativa das comunidades nos processos de comunicação; e 3) formas, conte��dos e objetivos que se complementam e dão identidade às experiências