Third-Generation-Cephalosporin-Resistant Klebsiella pneumoniae Isolates from Humans and Companion Animals in Switzerland: Spread of a DHA-Producing Sequence Type 11 Clone in a Veterinary Setting.

Characterization of third-generation-cephalosporin-resistant Klebsiella pneumoniae isolates originating mainly from one human hospital (n = 22) and one companion animal hospital (n = 25) in Bern (Switzerland) revealed the absence of epidemiological links between human and animal isolates. Human infections were not associated with the spread of any specific clone, while the majority of animal infections were due to K. pneumoniae sequence type 11 isolates producing plasmidic DHA AmpC. This clonal dissemination within the veterinary hospital emphasizes the need for effective infection control practices.

How Transcranial Direct Current Stimulation Can Modulate Implicit Motor Sequence Learning and Consolidation: A Brief Review

The purpose of this review is to investigate how transcranial direct current stimulation(tDCS)can modulate implicit motor sequence learning and consolidation. So far, most of the studies have focused on the modulating effect of tDCS for explicit motor learning. Here, we focus explicitly on implicit motor sequence learning and consolidation in order to improve our understanding about the potential of tDCS to affect this kind of unconscious learning. Specifically, we concentrate on studies with the serial reaction time task (SRTT), the classical paradigm for measuring implicit motor sequence learning. The influence of tDCS has been investigated for the primary motor cortex, the premotor cortex, the prefrontal cortex, and the cerebellum. The results indicate that tDCS above the primary motor cortex gives raise to the most consistent modulating effects for both implicit motor sequence learning and consolidation.

Fluorescence-based directed termination PCR: direct mutation characterization without sequencing

We describe a fluorescence-based directed termination PCR (fluorescent DT–PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concentrations. Visualization of resulting termination fragments is accomplished with a dual dye Li-cor DNA sequencer. As each DT–PCR reaction generates two sets of terminating fragments, a pair of complementary reactions with limiting dATP and dCTP collectively provide information on the entire sequence of a target DNA, allowing an accurate determination of any base change. Blind analysis of 78 mutants of the supF reporter gene using fluorescent DT–PCR not only correctly determined the nature and position of all types of substitution mutations in the supF gene, but also allowed rapid scanning of the signature sequences among identical mutations. The method provides simplicity in the generation of terminating fragments and 100% accuracy in mutation characterization. Fluorescent DT–PCR was successfully used to generate a UV-induced spectrum of mutations in the supF gene following replication on a single plate of human DNA repair-deficient cells. We anticipate that the automated DT–PCR method will serve as a cost-effective alternative to dideoxy sequencing in studies involving large-scale analysis for nucleotide sequence changes.

Dual transcriptional control by Ear3/COUP: negative regulation through the DR1 direct repeat and positive regulation through a sequence downstream of the transcriptional start site of the mouse mammary tumor virus promoter.

Ear3/COUP is an orphan member of the steroid/thyroid hormone receptor superfamily of transcription factors and binds most tightly to a direct repeat of AGGTCA with 1 nucleotide in between (DR1). Ear3/COUP also binds with a similar affinity to the palindromic thyroid hormone response element (TRE). This binding preference of Ear3/COUP is same as that of the retinoid X receptor (RXR), which is another member of the superfamily. In the present study, we identified a sequence responsible for Ear3/COUP-mediated transactivation in the region downstream of the transcription start site of the mouse mammary tumor virus promoter. This cis-acting sequence was unresponsive to RXR. When the DR1 or TRE sequence was added upstream of the promoter, transactivation by Ear3/COUP was completely abolished, whereas RXR enhanced transcription from the promoter. The mode of action of Ear3/COUP could be utilized to control complex gene expressions in morphogenesis, homeostasis, and development.

Direct support and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and special tools) : spectrum analyzer AN/UPM-58, FSN 6625-523-8576.

September 1973."

Organizational, direct support, and general support maintenance, repair parts and special tools list (including depot maintenance repair parts and special tools) for trainer, launch effects, guided missile M54, 6920-00-175-6327, pedestral,infrared transmitter M5, 6920-00-107-0667, monitoring set, guided missile system, training AN/TSQ-T1, 6920-00-165-6369 ...

Includes index.

Organizational, direct support and general support maintenance repair parts and special tools lists : spreader, aggregate, towed; 8 ft. spread, Garwood model M5-8FT serial numbers 101 thru 200 and 201 thru 374, NSN 3895-00-836-7324.

Includes index.

Unit, direct support, and general support maintenance repair parts and special tools lists : spreader, aggregate, towed; 8 ft. spread, Garwood model M5-8FT serial numbers 101 thru 200 and 201 thru 374, NSN 3895-00-836-7324.

"June 1992."

Organizational, direct support and general support maintenance repair parts and special tools list including depot maintenance repair parts and special tools for antenna-receiver-transmitter group, target ranging, trailer mounted AN/MPA-53, 1430-00-433-9143, improved Nike-Hercules Air Defense Guided Missile System.

Includes index.

Direct support and general support maintenance manual : receiver radio frequency noise measurement equipment including test set, radio frequency noise TS-3376/G (NSN 6625-00-237-2406), monitor, noise temperature ID-1915/G (NSN 5895-00-241-9367) for satellite communication terminal AN/FSC-78(V).

"June 1978."

Direct support and general support maintenance manual : translator, signal data CV-3098/G (NSN 5895-00-237-2472) for satellite communication terminal AN/FSC-78(V).

"June 1978."

Direct support and general support maintenance repair parts and special tools list (including depot maintenance repair parts and special tools) for receiver-transmitter radio RT-246/VRC, RT-246A/VRC, (NSN 5820-00-892-0623).

"31 January 1983."

Direct support and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and special tools) for receiver-transmitter, radio RT-505/PRC-25 (NSN 5820-00-857-0934).

Includes index.

Direct support and general support maintenance repair parts and special tools list (including depot maintenance repair parts and special tools) for receiver-transmitter radio RT-524/VRC and RT-524A/VRC (NSN 5820-00-892-0622).

Includes indexes.