Regulation of endothelial cell integrin function and angiogenesis by COX-2, cAMP and Protein Kinase A.

Angiogenesis, the development of new blood vessels from preexisting vessels, is a key step in tumor growth, invasion and metastasis formation. Inhibition of tumor angiogenesis is considered as an attractive approach to suppress cancer progression and spreading. Adhesion receptors of the integrin family promote tumor angiogenesis by mediating cell migration, proliferation and survival of angiogenic endothelial cells. Integrins up regulated and highly expressed on neovascular endothelial cells, such as alphaVbeta3 and alpha5beta1, have been considered as relevant targets for anti-angiogenic therapies. Small molecular integrin antagonists or blocking antibodies suppress angiogenesis and tumor progression in many animal models, and some of them are currently being tested in cancer clinical trials as anti-angiogenic agents. COX-2 inhibitors exert anti-cancer effects, at least in part, by inhibiting tumor angiogenesis. We have recently shown that COX-2 inhibitors suppress endothelial cell migration and angiogenesis by preventing alphaVbeta3-mediated and cAMP/PKA-dependent activation of the small GTPases Rac and Cdc42. Here we will review the evidence for the involvement of vascular integrins in mediating angiogenesis and the role of COX-2 metabolites in modulating the cAMP/Protein Kinase A pathway and alphaVbeta3-dependent Rac activation in endothelial cells.

Report on a special investigation of the Assistant Director of the Camp Adventure program at the University of Northern Iowa for the period January 1, 2006 through March 31, 2008

Report on a special investigation of the Assistant Director of the Camp Adventure program at the University of Northern Iowa for the period January 1, 2006 through March 31, 2008

cAMP-dependent chloride secretion mediates tubule enlargement and cyst formation by cultured mammalian collecting duct cells.

Polycystic kidney diseases result from disruption of the genetically defined program that controls the size and geometry of renal tubules. Cysts which frequently arise from the collecting duct (CD) result from cell proliferation and fluid secretion. From mCCD(cl1) cells, a differentiated mouse CD cell line, we isolated a clonal subpopulation (mCCD-N21) that retains morphogenetic capacity. When grown in three-dimensional gels, mCCD-N21 cells formed highly organized tubular structures consisting of a palisade of polarized epithelial cells surrounding a cylindrical lumen. Subsequent addition of cAMP-elevating agents (forskolin or cholera toxin) or of membrane-permeable cAMP analogs (CPT-cAMP) resulted in rapid and progressive dilatation of existing tubules, leading to the formation of cystlike structures. When grown on filters, mCCD-N21 cells exhibited a high transepithelial resistance as well as aldosterone- and/or vasopressin-induced amiloride-sensitive and -insensitive current. The latter was in part inhibited by Na(+)-K(+)-2Cl(-) cotransporter (bumetanide) and chloride channel (NPPB) inhibitors. Real-time PCR analysis confirmed the expression of NKCC1, the ubiquitous Na(+)-K(+)-2Cl(-) cotransporter and cystic fibrosis transmembrane regulator (CFTR) in mCCD-N21 cells. Tubule enlargement and cyst formation were prevented by inhibitors of Na(+)-K(+)-2Cl(-) cotransporters (bumetanide or ethacrynic acid) or CFTR (NPPB or CFTR inhibitor-172). These results further support the notion that cAMP signaling plays a key role in renal cyst formation, at least in part by promoting chloride-driven fluid secretion. This new in vitro model of tubule-to-cyst conversion affords a unique opportunity for investigating the molecular mechanisms that govern the architecture of epithelial tubes, as well as for dissecting the pathophysiological processes underlying cystic kidney diseases.

Més enllà de les filferrades: la resiliència i els infants de l'èxode republicà al camp de Rivesaltes

Treball de recerca realitzat per alumnes d’ensenyament secundari i guardonat amb un Premi CIRIT per fomentar l'esperit científic del Jovent l’any 2010. La recerca duta a terme pretén donar veu a persones que, essent infants, van patir el tancament en els camps de concentració del sud de França, i especialment el de Rivesaltes, a la fi de la Guerra Civil espanyola. S'intenta esbrinar si la situació traumàtica viscuda per aquests infants els hi ha permès, posteriorment, portar una vida satisfactòria, si han estat capaços de superar el record dur del seu tancament. L’objectiu és posar de manifest que els nens són les principals víctimes dels conflictes d'abans i d'ara i aquests conflictes poden deixar seqüeles que els dificultin dur una vida plena.

Jornada de Camp de l'Arròs 2013

L’arròs és el conreu amb més rellevància mediambiental, econòmica i social en el nostre territori. Aquesta jornada de camp ens ofereix l’oportunitat d’estar al dia dels avanços que es van introduint, a més de ser un lloc de trobada i intercanvi d’experiències entre tots els assistents. Enguany, com a novetat, presentem el programa Origens de Kellogg’s i els assajos d’estrès hídric de la Comunitat de Regants de la Dreta. A més, la vitrina tecnològica s’està consolidant com a eina per a difondre noves tecnologies. La visita als diferents assajos es durà a terme simultàniament, però amb grups reduïts. La jornada va adreçada a tots aquells arrossers i tècnics vinculats al món de l’arròs.

Mémoire sur le camp retranché de Barrault

Ancien possesseur : Argenson, Antoine-René de Voyer (1722-1787 ; marquis de Paulmy d')

Report on a special investigation of the University of Northern Iowa, Camp Adventure Youth Services program for the period April 1, 2007 through March 31, 2008

Report on a special investigation of the University of Northern Iowa, Camp Adventure Youth Services program for the period April 1, 2007 through March 31, 2008

Un edifici volcànic inèdit a Caldes de Malavella (la Selva, Girona): el volcà del camp dels Ninots

Es descriu la morfologia i els dipòsits del volcà inèdit del camp dels Ninots (Caldes de Malavella)

Complex poliesportiu de Camp Clar

Concurs d’avantprojectes del Complex Poliesportiu de Camp Clar

Remodelació del Nou Camp del F.C. Barcelona

Proposta presentada per al Concurs de Remodelació del Nou Camp del Futbol Club Barcelona.