Online exercise for the design and simulation of PCR and PCR-RFLP experiments

[EN] Background: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism of PCR products (PCR-RFLP) are extensively used molecular biology techniques. An exercise for the design and simulation of PCR and PCR-RFLP experiments will be a useful educational tool. Findings: An online PCR and PCR-RFLP exercise has been create that requires users to find the target genes,compare them, design primers, search for restriction endonucleases, and finally to simulate the experiment. Each user of the service is randomly assigned a gene from Escherichia coli; to complete the exercise, users must design an experiment capable of distinguishing among E. coli strains. By applying the experimental procedure to all completely sequenced E. coli, a basic understanding of strain comparison and clustering can also be acquired. Comparison of results obtained in different experiments is also very instructive. Conclusions: The exercise is freely available at http://insilico.ehu.es/edu.

Signposting for design process improvement

This report presents a dynamic approach to design process planning which aims to enable design process improvement. The tool utilises a signposting model to direct activity by suggesting the next most appropriate task in the design process. This suggestion is based on the presence of key parameters, their associated confidences and an assessment of the performance of the design process. The assessment approach proposed has the potential to adapt to the experience of the designer. A case study of mechanical component design is presented to illustrate the behaviour of this model for design process improvement.