Alterações hematológicas em cães (Canis lupus familiaris) após tratamento com eletroacupuntura

A acupuntura, é uma terapia que cada vez mais adquire uma credibilidade no mundo ocidental, a sua indicação pode ser para pacientes anêmicos e imunossuprimidos, e outras afecções. O experimento foi realizado com oito cães adultos, sendo 4 fêmeas e 4 machos. O estudo consistiu em análise das concentrações das células vermelhas e brancas avaliado em 4 tratamentos: controle (T0), acupuntura (T1), eletroacupuntura unilateral (T2) e eletroacupuntura bilateral (T3). Os acupontos utilizados foram intestino grosso 4, intestino grosso 11 e estômago 36. Todos os animais passaram por todos os tratamentos com um intervalo de 7 dias para cada tratamento. Os hemogramas séricos foram verificados 20 minutos antes do início do tratamento (M0), logo após o tratamento (M1), e 60 minutos (M2) e 120 minutos (M3) após o tratamento. O resultado da série vermelha não foi significativo, mas notou-se somente uma diminuição significativa da concentração média corpuscular (CHCM) (p<0,05) no tratamento T1 no M1 em comparação aos outros tratamentos; notou-se também uma redução significativa (p<0,05) na concentração plaquetária de T1, T2 e T3 em comparação ao grupo controle. A eletroacupuntura pode levar a uma trombocitopenia, quando estimulado estes pontos, provavelmente por um estímulo parassimpaticomimético.

Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil

Abstract:This study aimed to report the prevalence of Babesia canis vogeli in dogs and ticks in the urban and rural areas of Petrolina, Pernambuco. Serum and peripheral blood samples of 404 dogs were tested by indirect immunofluorescence assay (IFA) and by blood smears, respectively. The presence of tick infestation was evaluated, and some specimens were submitted to DNA amplification by polymerase chain reaction (PCR). The presence of antibodies anti-B. canis vogeli was determinate in 57.9% (234/404) of dogs. The direct detection of Babesia spp was obtained in 0.5% (2/404) dogs by visualization of intraerythrocytic forms. Infestation by Rhipicephalus sanguineus sensu lato was observed in 54.5% (220/404) of dogs in both urban and rural areas. DNA of Babesia canis vogeli were obtained by PCR in 6% individual (3/50) and 8.7% of pool of ticks (7/80). The risk factors for the presence of anti-B. canis vogeli antibodies, as determined through the application of logistic regression models (P<0.05), were the following: medium breed size variables (P<0.001); contact with areas of forest (P=0.021); and access on the street (P=0.046). This study describes, for the first time, the confirmation of infection of B. canis vogeli in dogs and ticks in the semiarid region of Pernambuco, Brazil.

Evidence for eosinophil recruitment, leukotriene B4 production and mast cell hyperplasia following Toxocara canis infection in rats

It is well known that eosinophilia is a key pathogenetic component of toxocariasis. The objective of the present study was to determine if there is an association between peritoneal and blood eosinophil influx, mast cell hyperplasia and leukotriene B4 (LTB4) production after Toxocara canis infection. Oral inoculation of 56-day-old Wistar rats (N = 5-7 per group) with 1000 embryonated eggs containing third-stage (L3) T. canis larvae led to a robust accumulation of total leukocytes in blood beginning on day 3 and peaking on day 18, mainly characterized by eosinophils and accompanied by higher serum LTB4 levels. At that time, we also noted increased eosinophil numbers in the peritoneal cavity. In addition, we observed increased peritoneal mast cell number in the peritoneal cavity, which correlated with the time course of eosinophilia during toxocariasis. We also demonstrated that mast cell hyperplasia in the intestines and lungs began soon after the T. canis larvae migrated to these compartments, reaching maximal levels on day 24, which correlated with the complete elimination of the parasite. Therefore, mast cells appear to be involved in peritoneal and blood eosinophil infiltration through an LTB4-dependent mechanism following T. canis infection in rats. Our data also demonstrate a tight association between larval migratory stages and intestinal and pulmonary mast cell hyperplasia in the toxocariasis model.

1.° Anonymi opusculum de memoria artificiali. — 2.° Joannis Jacobi Canis tractatus de eodem argumento. — 3.° Petri de Urbe Veteri libellus de eodem argumento. — 4.° Idem libellus, italico idiomate. — 5.° La memoria artificiale di Tulio. — 6.° Aristotelis ad Alexandrum liber de regimine vitae. — 7.° Rufi Sexti, viri Consularis, historiae Romanae breviarium. — 8.° Francisci Diedi, Legati, defensio pro Republica Veneta. — 9.° Benedicti, ordinis Praedicatorum, oratio in laudem sancti Thomae Aquinatis. — 10.° Bernardi Justiniani oratio pro militia. — 11.° Anonymi opusculum de pedibus metricis.

Mazarinaeus

Estado actual de la rabia, cobertura y porcentaje de vacunación antirrábica en perros Canis familiaris (L.) 1758 y gatos felis catus (L.)1758, durante 1997 y 1998, en el área limítrofe de la zona metropolitana de Monterrey, Nuevo León, México

Tesis (Maestría en Ciencias con Especialidad en Manejo de Vida Silvestre) UANL

Predación de coyote Canis latrans texensis en venado cola blanca Odocoileus virginianus texanus en Anáhuac, Nuevo León, México

Tesis (Maestría en Ciencias con Especialidad en Manejo de Vida Silvestre) UANL

Detección molecular y aislamiento de Brucella canis en perros del área metropolitana de Monterrey, N.L.

Tesis (Maestro en Ciencias Veterinarias) U.A.N.L., 2006

Lesiones en el parénquima pulmonar causadas por diferentes agentes etiológicos en coyotes (canis latrans) del norte de México.

Tesis (Maestría en Ciencia Animal) UANL, 2014.

Métodos de criopreservación de huevos embrionadod de toxocara canis.

Tesis (Maestría en Ciencia Animal) UANL, 2014.

Canis Volans

Creación de marca de accesorios de moda, arte y diseño.

Avaliação de Índices de Função Sistólica do Ventrículo Esquerdo em Canis lupus familiaris por Técnica de Ecocardiografia Doppler

A função cardíaca é fortemente condicionada pela mioarquitectura do coração, pelo que o estudo da relação morfologia-função ventricular nos mamíferos é de grande importância no diagnóstico e tratamento de patologias cardíacas. O cálculo dos volumes cardíacos durante todo o ciclo cardíaco tem uma importância fundamental para o estudo da função ventricular e para a definição dos padrões hemodinâmicos. Foi objectivo deste trabalho, o estudo de índices de função sistólica ventricular esquerda, em 25 cães (Cannis lupus familiaris), através da utilização de diferentes técnicas ecocardiográficas, como o Modo M, ecocardiografia bidimensional, Doppler tecidular e a ecocardiografia de contraste. Foi utilizado contraste ultrasonográfico, para melhorar a definição do endocárdio e possibilitar maior rigor no cálculo dos volumes ventriculares, e na avaliação da função ventricular sisto-diastólica global e regional. Os resultados apresentados foram sujeitos a tratamento estatístico com software Analyze-it. O Doppler tecidular mostrou ser um método robusto para avaliar a função sistólica. A ecocardiografia de contraste permitiu não só obter uma melhor definição endocárdica, como obter valores de fracção de ejecção com diferença estatisticamente significativa. Como os três métodos utilizados avaliam diferentes aspectos da função sistólica, função ventricular radial, circunferencial e longitudinal, não podem ser comparados directamente entre si.

Description of Enterococcus canis sp nov from dogs and reclassification of Enterococcus porcinus Teixeira et al. 2001 as a junior synonym of Enterococcus villorum Vancanneyt et al. 2001

Strains from anal swabs and chronic otitis externa in dogs were shown to be phylogenetically related to the Enterococcus faecium species group. They shared a number of phenotypic characteristics with these species, but they could be easily differentiated by biochemical reactions. In addition, the canine strains were unusual in their nearly complete failure to grow on sodium azide-containing enterococci-selective media and in their Voges-Proskauer reactions (usually negative). By using 16S rRNA sequencing and DNA-DNA hybridization of representative strains, as well as tDNA interspacer gene PCR and SDS-PAGE of whole-cell proteins, the group of canine strains was shown to constitute a novel enterococcal species. The name Enterococcus canis sp. nov. is proposed for this species, with LMG 12316(T) (= CCUG 46666(T)) as the type strain. Concurrently, the taxonomic situation and nomenclatural position of Enterococcus porcinus were investigated. As no phenotypic or genotypic differences were found between this species and Enterococcus villorum, the name E. porcinus is considered to be a junior synonym of E. villorum.

Actinomyces canis sp. nov., isolated from dogs

Three strains of a previously undescribed catalase-positive Actinomyces-like bacterium were isolated from dogs. Biochemical testing and PAGE analysis of whole-cell proteins indicated that the strains were phenotypically highly related to each other but different from previously described Actinomyces and Arcanobacterium species. Sequencing of 16S rRNA showed that the unknown bacterium represents a new subline within a cluster of species which includes Actinomyces hyovaginalis, Actinomyces georgiae, Actinomyces meyeri, Actinomyces odontolyticus, Actinomyces radingae and Actinomyces turicensis. On the basis of phenotypic evidence and 16S rRNA sequence divergence levels (greater than 5% with recognized Actinomyces species) it is proposed that the unknown strains from canine sources be classified as a new species with the name Actinomyces canis sp. nov. The type strain of Actinomyces canis is CCUG 41706T (= CIP 106351T).