O pós-moderno e a relação entre literatura e história em Running dog, de Don Delillo

Pós-graduação em Letras - IBILCE

Phase I clinical and pharmacokinetic evaluation of the vascular-disrupting agent OXi4503 in patients with advanced solid tumors

Preclinical studies show that OXi4503 (combretastatin A1 diphosphate, CA1P) is more potent than other clinically evaluated vascular-disrupting agents.

Abrogation of the alternative complement pathway by targeted deletion of murine factor B

To investigate the role of complement protein factor B (Bf) and alternative pathway activity in vivo, and to test the hypothesized potential genetic lethal effect of Bf deficiency, the murine Bf gene was interrupted by exchange of exon 3 through exon 7 (including the factor D cleaving site) with the neor gene. Mice heterozygous for the targeted Bf allele were interbred, yielding Bf-deficient offspring after the F1 generation at a frequency suggesting that Bf deficiency alone has no major effect on fertility or fetal development. However, in the context of one or more genes derived from the 129 mouse strain, offspring homozygous for Bf deficiency were generated at less than expected numbers (P = 0.012). Bf-deficient mice showed no gross phenotypic difference from wild-type littermates. Sera from Bf-deficient mice lacked detectable alternative complement pathway activity; purified mouse Bf overcame the deficit. Classical pathway-dependent total hemolytic activity was lower in Bf-deficient than wild-type mice, possibly reflecting loss of the alternative pathway amplification loop. Lymphoid organ structure and IgG1 antibody response to a T-dependent antigen appeared normal in Bf-deficient mice. Sensitivity to lethal endotoxic shock was not significantly altered in Bf-deficient mice. Thus, deficiency of Bf and alternative complement activation pathway led to a less dramatic phenotype than expected. Nevertheless, these mice provide an excellent model for the assessment of the role of Bf and the alternative pathway in host defense and other functions in vivo.

Requirement for membrane lymphotoxin in natural killer cell development

Development of natural killer (NK) cells is thought to depend on interactions between NK progenitors and the bone marrow (BM) microenvironment; however, little is known about the molecular signals involved. Here we show that lymphotoxin (LT) provides an important signal for the development of both NK cells and NK/T cells. LTα−/− mice show marked reduction in splenic and BM NK and NK/T cell numbers and dramatically impaired NK and NK/T cell function. Mice deficient in either tumor necrosis factor receptor (TNFR)-I or TNFR-II have normal numbers of NK and NK/T cells, implying that neither of the TNFRs nor soluble LTα3 is required for development of these cell types. Reciprocal BM transfers between LTα−/− and wild-type mice suggest that close interactions between membrane LT-expressing NK cell precursors and LT-responsive radioresistant stromal cells are necessary for NK cell development. When LT-deficient BM cells are incubated with IL-15, NK cells are formed. In addition, LT-deficient BM cells produce IL-15 after activation. Thus, membrane LT appears to deliver a signal for NK cell development that is either independent of IL-15 or upstream in the IL-15 pathway. These results reveal a novel function for membrane LT in NK and NK/T cell development. They also support a cellular and molecular mechanism by which NK cell precursors themselves deliver essential signals, through the membrane ligand, that induce the microenvironment to promote further NK cell and NK/T cell development.

Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2.

Complement receptor 1 (CR1, CD35) and complement receptor 2 (CR2, CD21) have been implicated as regulators of B-cell activation. We explored the role of these receptors in the development of humoral immunity by generating CR1- and CR2-deficient mice using gene-targeting techniques. These mice have normal basal levels of IgM and of IgG isotypes. B- and T-cell development are overtly normal. Nevertheless, B-cell responses to low and high doses of a T-cell-dependent antigen are impaired with decreased titers of antigen-specific IgM and IgG isotypes. This defect is not complete because there is still partial activation of B lymphocytes during the primary immune response, with generation of splenic germinal centers and a detectable, although reduced, secondary antibody response. These data suggest that certain T-dependent antigens manifest an absolute dependence on complement receptors for the initiation of a normally robust immune response.

The leukemia-associated-protein (LAP) domain, a cysteine-rich motif, is present in a wide range of proteins, including MLL, AF10, and MLLT6 proteins.

We have identified and further characterized a Caenorhabditis elegans gene, CEZF, that encodes a protein with substantial homology to the zinc finger and leucine zipper motifs of the human gene products AF10, MLLT6, and BR140. The first part of the zinc finger region of CEZF has strong similarity to the corresponding regions of AF10 (66%) and MLLT6 (64%) at the cDNA level. As this region is structurally different from previously described zinc finger motifs, sequence homology searches were done. Twenty-five other proteins with a similar motif were identified. Because the functional domain of this motif is potentially disrupted in leukemia-associated chromosomal translocations, we propose the name of leukemia-associated protein (LAP) finger. On the basis of these comparisons, the LAP domain consensus sequence is Cys1-Xaa1-2-Cys2-Xaa9-21-Cys3-Xaa2-4 -Cys4-Xaa4-5-His5-Xaa2-Cys6-Xaa12-46 - Cys7-Xaa2-Cys8, where subscripted numbers represent the number of amino acid residues. We review the evidence that this motif binds zinc, is the important DNA-binding domain in this group of regulatory proteins, and may be involved in leukemogenesis.

[Court appointment to inventory the Gannett estate], 1818 April 29

A one-page printed Middlesex County Court of Probate form appointing and authorizing William Hilliard, James P. Chaplin, and Royal Morse to inventory of the estate of Caleb Gannett.

An inventory of all of the estate of Caleb Gannett late of Cambridge in the county of Middlesex, Esquire, deceased, appraised upon oath by us the subscribers, duly appointed to that service by the Hon. James Prescott Esq. Judge of Probate & wills, in said county of Middlesex, viz., 1818 May 21

Eight-page handwritten inventory and appraisal of Caleb Gannett's real estate and personal estate by William Hilliard, James R. Chaplin, and Royal Morse with an attached certification of the Middlesex County Court of Probate signed May 26, 1818.

Letters from William Tudor to John Thornton Kirkland,

Three letters, one in which Tudor suggests persuading the Episcopal Church to send a bishop to reside in Cambridge and establish a divinity professorship as a means to attract students from other states who are wary of Unitarianism. Tudor also makes inquiries regarding the title of Doctor for a Reverend Chaplin and asks about college records of James Otis.

Windham County, Connecticut, 1856 (Image 4 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of Windham County, Connecticut, from actual survey by E.P. Gerrish, W.C. Eaton & D.S. & H.C. Osborn, 1855; lith by W.H. Rease. It was published by E.M. Woodford in 1856. Scale [ca. 1:42,240]. This layer is image 4 of 4 total images, representing the northwest portion of the four sheet source map.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Connecticut State Plane coordinate system (NAD 1983 in Feet) (Fipszone 0600). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, public buildings, schools, churches, cemeteries, industry locations (e.g. mills, factories, mines, etc.), private buildings with names of property owners, town boundaries, and more. Relief shown by hachures. It includes many cadastral insets of individual county towns and villages. It also includes illustrations, business directories, and tables of statistics and distances.This layer is part of a selection of digitally scanned and georeferenced historic maps of New England from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates, scales, and map purposes.

Windham County, Connecticut, 1856 (Image 3 of 4) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of Windham County, Connecticut, from actual survey by E.P. Gerrish, W.C. Eaton & D.S. & H.C. Osborn, 1855; lith by W.H. Rease. It was published by E.M. Woodford in 1856. Scale [ca. 1:42,240]. This layer is image 3 of 4 total images, representing the southwest portion of the four sheet source map.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Connecticut State Plane coordinate system (NAD 1983 in Feet) (Fipszone 0600). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, public buildings, schools, churches, cemeteries, industry locations (e.g. mills, factories, mines, etc.), private buildings with names of property owners, town boundaries, and more. Relief shown by hachures. It includes many cadastral insets of individual county towns and villages. It also includes illustrations, business directories, and tables of statistics and distances.This layer is part of a selection of digitally scanned and georeferenced historic maps of New England from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates, scales, and map purposes.

(Table 2) Facies and grain-size description for Mississippi Fan sediments of DSDP Leg 96

Eight lithologic facies recognized in the Mississippi Fan sediments drilled during DSDP Leg 96 are defined on the basis of lithology, sedimentary structures, composition, and texture. Of these, the calcareous biogenic sediments are of minor importance, volumetrically, as compared with the dominant resedimented terrigenous facies. Clay, mud, and silt are the most abundant sediments at all the sites drilled, with some sand and gravel in the midfan channel fill and an abundance of sand on the lower fan. Facies distribution and vertical sequences reflect the importance of sediment type and supply in controlling fan development. Sea-level changes and diapiric activity have also played an important role. Clay and sand fraction mineralogy closely mirror the dominant sediment source, namely, the Mississippi River system and adjacent continental shelf. Local and regional variation in composition on the fan mostly reflects facies differences.

The Hidden name : a play for girls ...

Mode of access: Internet.

The New England theocracy. A history of the Congregationalists in New England to the revivals of 1740,

Translation of Geschichte der congregationalisten in Neu-England bis zu den erweckungen um das jahr 1740.

Applications of industrial pH control.

Mode of access: Internet.