Molecular investigation of zoonotic genotypes of Giardia intestinalis isolates in humans, dogs and cats, sheep, goats and cattle in Araçatuba (Sãoo Paulo State, Brazil) by the analysis of beta-giardin gene fragments

In the period from July 2009 to October 2010, fecal samples from 61 animals and 154 humans from the municipality of Aracatuba (São Paulo State, Brazil) were studied. Fecal samples from animals were collected in the Municipal Animal Shelter and the Veterinary Hospital of the Universidade Estadual Paulista. Human fecal specimens were collected in playschools in the outskirts of the city by the private network of clinical analysis laboratories of the municipal. Diagnosis was done by optical microscopy using the Faust and Hoffmann, Pons and Janer techniques. The genotypes of Giardia intestinalis were characterized by PCR-RFLP and confirmed by sequencing the ß-giardin gene. Human specimens were positive in 25.3% (39/154) of the cases with 26.8% (36/134) of the specimens from children and 15% (3/20) from adults being positive. The frequency of G. intestinalis among the animals was 23.0% (14/61). A total of 32 isolates of G. intestinalis obtained from human feces and six from dogs and cats were characteristic of the A genotype (AI and AII/AIII). The results of this study in respect to frequency of giardiasis are similar to reported in most studies in Brazil. The prevalence observed in animal populations conforms to worldwide infection rates. G. intestinalis genotypes considered zoonotic were detected in both pets and humans from the city of Aractuba, suggesting a possible zoonotic transmission of the parasite in the northwestern region of São Paulo State. The absence of these genotypes in farm animals may imply that they are not involved in the chain of transmission to humans in this region.

The frequency of HLA-DRB1 polymorphisms in Brazilian Plasmodium vivax malaria patients and in blood donors from the Amazon Region

We evaluated the frequency of different HLA-DRB1 alleles in Plasmodium vivax-infected individuals and in healthy blood donors from malaria endemic areas of Brazil. Low-resolution human leukocyte antigen-DRB1 genotyping was performed for 73 malaria patients and 29 healthy blood donors. The most frequent alleles in individuals from northern Brazil were human leukocyte antigen-DRB1*04, *08, *07 and *13. The frequency of human leukocyte antigen-DRB1*07 was higher in malaria-infected individuals than in the control group, which reinforces the theory that this allele plays an important role in susceptibility to malaria. This study offers new information about a potential susceptibility factor for P. vivax malaria in a Brazilian population that is naturally exposed to malaria.

A apropriação das obras de Ricardo Antunes pelo Serviço Social: a categoria trabalho em debate

Pós-graduação em Serviço Social - FCHS

Humoral immune responses against the malaria vaccine candidate antigen Plasmodium vivax AMA-1 and IL-4 gene polymorphisms in individuals living in an endemic area of the Brazilian Amazon

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comparação de três métodos na retificação cilíndrica do aço ABNT 52100

No processo de retificação, a lubrificação no contato da ferramenta (rebolo) com a peça e a refrigeração da superfície usinada visa melhor acabamento final. Este trabalho apresenta como alternativa ao método convencional de lubrificação/refrigeração (fluido abundante) a técnica MQL em duas condições: pura e com adição de água. Foram avaliadas a eficiência e a eficácia desses métodos no processo de retificação cilíndrica externa do aço ABNT 52100 com o rebolo de óxido de alumínio. Apesar de desgastar um maior volume de rebolo e resultar em um maior desvio de circularidade na peça, as técnicas de MQL puro e MQL com adição de água apresentaram os melhores valores de rugosidade, o que os tornam alternativas ao método convencional.

Influence of HLA-DRB-1 alleles on the production of antibody against CSP, MSP-1, AMA-1, and DBP in Brazilian individuals naturally infected with Plasmodium vivax

We evaluated the influence of allelic frequency of the human leukocyte antigen (HLA) -DRB1 on the acquisition of antibody response against malaria sporozoite and merozoite peptides in patients with Plasmodium vivax malaria acquired in endemic areas of Brazil. IgG antibodies were detected by enzyme-linked immunosorbent assay against four peptides of circumsporozoite protein (CSP) (amino, carboxyl, and VK210 and VK247 repeats) and peptides of merozoite surface protein 1 (MSP-1), apical membrane antigen 1 (AMA-1), and Duffy-binding protein (DBP). We found an association between HLA-DR3 and HLA-DR5 alleles and lack of antibody response to CSP amino terminal, as well as an association between HILA-DR3 and the highest antibody response to MSP1 (Pv200L). In conclusion, we suggest a potential regulatory role of the H1A-DRB1 alleles in the production of antibodies to a conserved region of P. vivax CSP and MSP1 in Brazilian population exposed to malaria. (C) 2011 Elsevier B.V. All rights reserved.

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.

Historia, literatura y lengua en el epistolario de Ricardo Palma y Miguel De Unamuno

En el presente trabajo partimos de una clara ponderación de la escritura epistolar como espacio gnoseológico que surge de la relación entre Ricardo Palma y Miguel de Unamuno, entre los años 1903 y 1913. Por tanto se inscribe, asimismo, en el campo de estudio de las relaciones culturales entre España y América. Como consecuencia, nuestro propósito principal no supone un abordaje solamente intratextual sino intertextual de la obra de Palma y la de Unamuno, en aquellos textos en los que se cruzan, ya sea bajo la forma de prólogos, comentarios o sencillamente en el intercambio epistolar. En marco esbozado, leemos una serie de problemáticas que se ocupan de la historia, la literatura y la lengua.

Juan José Saer – Ricardo Suspisiche: cruce de lenguajes para la creación de un espacio

Una experiencia vivida y comentada por el propio Suspisiche -pintor del litoral santafecino- y la frecuentación de los textos saerianos abren la posibilidad de este trabajo que pretende un acercamiento al cruce de lenguajes entre las obras de ambos. Cada uno desde su propia estética genera un espacio que trasciende la referencialidad para convertirse en una poética, pero también en una verdadera cosmovisión. Las obras de Saer van develando su concepción de la literatura y del mundo, concepción según la cual la realidad es huidiza, imposible de aprehender. Esta certeza conduce su proceso de escritura. aporta sus tópicos recurrentes que no son más que la insistencia desesperada por comunicar las propias percepciones. De la pintura de Suspisiche, dijo el crítico Taverna Irigoyen: "es un paisaje que pareciera entregársele fácilmente, y cuando lo va a hacer suyo, a penetrarlo, se convierte en un espejismo inatrapable". ¿Cómo resuelven ambos. en la superficie textual. esa desconfianza en la posibilidad de instaurar sus universos? Por el camino del lenguaje, por la experimentación con que pueden transformar, superponer, reiterar, hasta crear un significado. Nuestro trabajo trata de demostrar cómo ambos evolucionan hacia la esencialidad que se logra por la progresiva supresión de lo accesorio, el abandono del pintoresquismo y una praxis que exige técnicas cada vez más rigurosas. Para el/o ese analiza el proceso de productividad de esos espacios que envuelven el trayecto vital del hombre y son uno con él, no por afinidades psicologistas. a la manera romántica, sino fatalmente, por impregnación. por simbiosis, por acoplamiento. No es el hombre el que se apropia del paisaje. es el espacio el que lo contiene. y permite al artista. desde adentro. una mirada que no es sólo recreación estética. sino ideología. en el sentido de visión del mundo.

Ponte, Jorge Ricardo, La fragilidad de la memoria. Representaciones, prensa y poder de una ciudad latinoamericana en tiempos del modernismo. Mendoza, 1885/1910. Mendoza, Ed. Fundación CRICYT, 1999,450 p.

Fil: Arpini, Adriana.

Ricardo Rojas y “La Literatura Argentina" en 1913 : universidad, estudios literarios y proyección político-social

Fil: Dubatti, Jorge.