O cinema de Manoel de Oliveira: um caso singular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A dramaturgia de Dea Lohe na peça Inocência: o hibridismo teatral na cena contemporânea

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Teatro e a dialética das linguagens: Cia Teatral Bumba Meu Baco

Este trabalho é centrado na análise específica das vivências teatrais do grupo “Cia. Bumba meu Baco”, projeto reconhecido pela PROEX desde 2006, do Campus da UNESP de Rio Claro. Desta forma, utiliza-se das experiências vividas pelo grupo durante todo o período de sua criação até o presente momento, para demonstrar como a linguagem teatral trata de assuntos da ciência geográfica e como ela pode chegar a gerar discussões e resultados no âmbito geográfico. Para isso, analisa-se em um primeiro momento a evolução da companhia, que ao longo dos anos pesquisou e trabalhou diferentes temas chegando ao seu trabalho mais relevante no ano de 2009, que utilizou como um dos métodos o teatro épico de Bertolt Brecht. Com o cunho político e social que possui, tal método ajudou o grupo a expandir a relação entre arte cênica e temas em geografia humana, além de gerar outros acréscimos a todos os que participaram direta e indiretamente deste processo

Dramaturgia de Dea Loher na peça Inocência: o hibridismo teatral na cena contemporânea

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Futebol, poesia e drama: o espetáculo

O que seria necessário para que se pudesse dizer que uma partida de futebol semelha de alguma forma a um espetáculo teatral, poético, emocional? O artigo propõe um passeio livre pela história dos diferentes tipos de teatro e pelas mudanças sofridas ao longo dos anos. A autora lembra que existe, da metade do século passado para cá, uma nítida divisão que colocou em campos opostos o teatro dramático e o teatro épico, o drama rigoroso contra o texto quase absolutamente livre, Aristóteles contra Brecht. E volta a apresentar as razões pela qual se pode afirmar que existem certos momentos no futebol que o aproximam ainda mais do espetáculo teatral, da dança, da comédia e do drama

Toxicity of clopidogrel and ticlopidine on human myeloid progenitor cells: importance of metabolites

Ticlopidine and clopidogrel are thienopyridine derivatives used for inhibition of platelet aggregation. Not only hepatotoxicity, but also bone marrow toxicity may limit their use. Aims of the study were to find out whether non-metabolized drug and/or metabolites are responsible for myelotoxicity and whether the inactive clopidogrel metabolite clopidogrel carboxylate contributes to myelotoxicity. We used myeloid progenitor cells isolated from human umbilical cord blood in a colony-forming unit assay to assess cytotoxicity. Degradation of clopidogrel, clopidogrel carboxylate or ticlopidine (studied at 10 and 100 μM) was monitored using LC/MS. Clopidogrel and ticlopidine were both dose-dependently cytotoxic starting at 10 μM. This was not the case for the major clopidogrel metabolite clopidogrel carboxylate. Pre-incubation with recombinant human CYP3A4 not only caused degradation of clopidogrel and ticlopidine, but also increased cytotoxicity. In contrast, clopidogrel carboxylate was not metabolized by recombinant human CYP3A4. Pre-incubation with freshly isolated human granulocytes was not only associated with a myeloperoxidase-dependent degradation of clopidogrel, clopidogrel carboxylate and ticlopidine, but also with dose-dependent cytotoxicity of these compounds starting at 10 μM. In conclusion, both non-metabolized clopidogrel and ticlopidine as well as metabolites of these compounds are toxic towards myeloid progenitor cells. Taking exposure data in humans into account, the myelotoxic element of clopidogrel therapy is likely to be secondary to the formation of metabolites from clopidogrel carboxylate by myeloperoxidase. Concerning ticlopidine, both the parent compound and metabolites formed by myeloperoxidase may be myelotoxic in vivo. The molecular mechanisms of cytotoxicity have to be investigated in further studies.

Hepatocellular toxicity of clopidogrel: Mechanisms and risk factors

Clopidogrel is a prodrug used widely as a platelet aggregation inhibitor. After intestinal absorption, approximately 90% is converted to inactive clopidogrel carboxylate and 10% via a two-step procedure to the active metabolite containing a mercapto group. Hepatotoxicity is a rare but potentially serious adverse reaction associated with clopidogrel. The aim of this study was to find out the mechanisms and susceptibility factors for clopidogrel-associated hepatotoxicity. In primary human hepatocytes, clopidogrel (10 and 100μM) was cytotoxic only after cytochrome P450 (CYP) induction by rifampicin. Clopidogrel (10 and 100μM) was also toxic for HepG2 cells expressing human CYP3A4 (HepG2/CYP3A4) and HepG2 cells co-incubated with CYP3A4 supersomes (HepG2/CYP3A4 supersome), but not for wild-type HepG2 cells (HepG2/wt). Clopidogrel (100μM) decreased the cellular glutathione content in HepG2/CYP3A4 supersome and triggered an oxidative stress reaction (10 and 100µM) in HepG2/CYP3A4, but not in HepG2/wt. Glutathione depletion significantly increased the cytotoxicity of clopidogrel (10 and 100µM) in HepG2/CYP3A4 supersome. Co-incubation with 1μM ketoconazole or 10mM glutathione almost completely prevented the cytotoxic effect of clopidogrel in HepG2/CYP3A4 and HepG2/CYP3A4 supersome. HepG2/CYP3A4 incubated with 100μM clopidogrel showed mitochondrial damage and cytochrome c release, eventually promoting apoptosis and/or necrosis. In contrast to clopidogrel, clopidogrel carboxylate was not toxic for HepG2/wt or HepG2/CYP3A4 up to 100µM. In conclusion, clopidogrel incubated with CYP3A4 is associated with the formation of metabolites that are toxic for hepatocytes and can be trapped by glutathione. High CYP3A4 activity and low cellular glutathione stores may be risk factors for clopidogrel-associated hepatocellular toxicity.

Toxicity of thienopyridines on human neutrophil granulocytes and lymphocytes

Thienopyridines can cause neutropenia and agranulocytosis. The aim of the current investigations was to compare cytotoxicity of ticlopidine, clopidogrel, clopidogrel carboxylate and prasugrel for human neutrophil granulocytes with the toxicity for lymphocytes and to investigate underlying mechanisms. For granulocytes, clopidogrel, ticlopidine, clopidogrel carboxylate and prasugrel were concentration-dependently toxic starting at 10μM. Cytotoxicity could be prevented by the myeloperoxidase inhibitor rutin, but not by the cytochrome P450 inhibitor ketoconazole. All compounds were also toxic for lymphocytes, but cytotoxicity started at 100μM and could not be prevented by rutin or ketoconazole. Granulocytes metabolized ticlopidine, clopidogrel, clopidogrel carboxylate and prasugrel, and metabolization was inhibited by rutin, but not by ketoconazole. Metabolism of these compounds by lymphocytes was much slower and could not be inhibited by ketoconazole or rutin. In neutrophils, all compounds investigated decreased the electrical potential across the inner mitochondrial membrane, were associated with cellular accumulation of ROS, mitochondrial loss of cytochrome c and induction of apoptosis starting at 10μM. All of these effects could be inhibited by rutin, but not by ketoconazole. Similar findings were obtained in lymphocytes; but compared to neutrophils, the effects were detectable only at higher concentrations and were not inhibited by rutin. In conclusion, ticlopidine, clopidogrel, clopidogrel carboxylate and prasugrel are toxic for both granulocytes and lymphocytes. In granulocytes, cytotoxicity is more accentuated than in lymphocytes and depends on metabolization by myeloperoxidase. These findings suggest a mitochondrial mechanism for cytotoxicity for both myeloperoxidase-associated metabolites and, at higher concentrations, also for the parent compounds.

Wiener Juden-Album für das Jahr 1852 : den p.t. Herren Juden ... mit bes. Zärtlichkeit gewiedmet

von Bertold

Yeḥefehfiyah

Bertold Oyerbakh [[Elektronische Ressource]]

Ms. lat. oct. 54 - Responsionale secundum usum ecclesiae S. Bartholomaei Francfordensis

Vorbesitzer: Jakob Butsch; Bertold Diller; Johannes Adam Mohr

De las utopías en el teatro setentista : circulación y recepción del repertorio brechtiano y de la creación colectiva en Mendoza (1968-1976)

En el presente trabajo, nos proponemos la reconstrucción de la realidad cultural generada en la provincia por las instituciones y agentes legitimantes del campo teatral (Bourdieu:135) y el posterior abordaje de la circulación y recepción "productiva" y "reproductiva" (Gimm, 1977:142) de las textualidades brechtianas y del teatro barrial de creación colectiva mediante una perspectiva metodológica comparatistica. Para ello analizaremos el discurso metateatral de la critica periodística y las puestas escénicas de Cristóbal Arnold y Ernesto Suárez, con la pretensión de destacar los rasgos de apropiación originales.

Los establos de su majestad : Fernando Lorenzo y Alberto Rodríguez(h) : reconstrucción histórica de la puesta en escena

Recuperar las raíces artísticas y teatrales es valorar y consolidar la esencia de nuestras formas y necesidades de expresión. La reconstrucción histórica de Los establos de su Majestad, respondió al propósito de investigar los parámetros ideológicos, estructurales y metodológicos de un espectáculo local, inmerso en una época determinada por la presencia de fuertes y fieles ideales políticos y sociales. Se decidió entonces estudiar especialmente los procesos de búsqueda y gestación de la puesta en escena realizada por un grupo de hacedores teatrales que bregaron, en esta ocasión, por la denuncia y el desenmascaramiento de la verdad a través de su creación artística. Considerando siempre al hecho teatral como síntesis y reflejo de un hecho social.