Role of dietary fats in modulating cardiometabolic risk during moderate weight gain : a randomized double-blind overfeeding trial (LIPOGAIN study)

BACKGROUND: Whether the type of dietary fat could alter cardiometabolic responses to a hypercaloric diet is unknown. In addition, subclinical cardiometabolic consequences of moderate weight gain require further study. METHODS AND RESULTS: In a 7-week, double-blind, parallel-group, randomized controlled trial, 39 healthy, lean individuals (mean age of 27±4) consumed muffins (51% of energy [%E] from fat and 44%E refined carbohydrates) providing 750 kcal/day added to their habitual diets. All muffins had identical contents, except for type of fat; sunflower oil rich in polyunsaturated fatty acids (PUFA diet) or palm oil rich in saturated fatty acids (SFA diet). Despite comparable weight gain in the 2 groups, total: high-density lipoprotein (HDL) cholesterol, low-density lipoprotein:HDL cholesterol, and apolipoprotein B:AI ratios decreased during the PUFA versus the SFA diet (-0.37±0.59 versus +0.07±0.29, -0.31±0.49 versus +0.05±0.28, and -0.07±0.11 versus +0.01±0.07, P=0.003, P=0.007, and P=0.01 for between-group differences), whereas no significant differences were observed for other cardiometabolic risk markers. In the whole group (ie, independently of fat type), body weight increased (+2.2%, P<0.001) together with increased plasma proinsulin (+21%, P=0.007), insulin (+17%, P=0.003), proprotein convertase subtilisin/kexin type 9, (+9%, P=0.008) fibroblast growth factor-21 (+31%, P=0.04), endothelial markers vascular cell adhesion molecule-1, intercellular adhesion molecule-1, and E-selectin (+9, +5, and +10%, respectively, P<0.01 for all), whereas nonesterified fatty acids decreased (-28%, P=0.001). CONCLUSIONS: Excess energy from PUFA versus SFA reduces atherogenic lipoproteins. Modest weight gain in young individuals induces hyperproinsulinemia and increases biomarkers of endothelial dysfunction, effects that may be partly outweighed by the lipid-lowering effects of PUFA. CLINICAL TRIAL REGISTRATION URL: http://ClinicalTrials.gov. Unique identifier: NCT01427140.

O processo de execução orçamentária no governo do Estado do Maranhão: um estudo de caso na universidade estadual do Maranhão

Este estudo trata do processo de execução do orçamento, referente ao período de 1995 a 2008, da Universidade Estadual do Maranhão, órgão de natureza autárquica, vinculado à Secretaria de Estado da Ciência, Tecnologia, Educação Superior e Desenvolvimento Tecnológico. O objetivo central desta pesquisa foi verificar a existência de pressão orçamentária naquela Instituição e, se existente, demonstrar quais os critérios relevantes na alocação das cotas e quais elementos geram pressão na elaboração e execução do orçamento, através da análise de dados institucionais e entrevistas de servidores ligados à sua Administração Superior e controle orçamentário, mediante a comparação do que fora orçado com o efetivamente realizado, em seus grupos de despesas, ou seja, pessoal, custeio e capital. Para tanto, o problema desta pesquisa foi descrito através das seguintes questões: Há pressão orçamentária na UEMA, na fase de elaboração do orçamento? Há pressão orçamentária na UEMA, na fase de execução do orçamento? Que elementos geram pressão na elaboração e na execução do orçamento da UEMA? Quais são os critérios relevantes à alocação das cotas orçamentárias entre os Centros de Ensino da UEMA? A coleta de dados foi realizada através de entrevistas semi-estruturadas e documentos. O processo de análise de dados consistiu em verificar documentos referentes à dotação orçamentária comparativamente as cotas liberadas e a análise em profundidade dos depoimentos colhidos nas entrevistas. A discussão dos resultados gerou conclusões importantes por constatar a existência de pressão orçamentária na elaboração e na execução do orçamento daquela Universidade, identificando os elementos causadores da pressão, assim como os critérios relevantes na alocação das cotas.

Poder judiciário fluminense e capixaba diante do comércio internacional e do pré-sal: novos desafios

As transformações recentes das relações sociais e econômicas incrementados pelo dinamismo do comércio internacional, sua crescente complexidade e as recémdescobertas reservas petrolíferas na camada pré-sal, aliadas ao fenômeno da globalização, contribuem para o processo de internacionalização do Direito. Esse cenário tem demandado, ainda, novos instrumentos de solução dos conflitos e de controle social, exigindo dos magistrados brasileiros maior expertise no julgamento das demandas contemporâneas. Diante desse cenário, é necessário construir alternativas que realmente busquem modificar as estruturas vigentes na teoria, na práxis e no ensino do Direito. Assim, o objetivo geral desta pesquisa é contribuir para a eficiência da prestação jurisdicional dos magistrados, seja no âmbito estadual ou federal, que atuam no Estado do Rio de Janeiro e Espírito Santo, diante dos novos desafios que terão em face do comércio internacional e do pré-sal. Com a finalidade de alcançar seu escopo, a pesquisa, no Capítulo 1, trata dos conceitos, princípios e metodologia para atingir o objetivo geral da pesquisa. O Capítulo 2 ocupa-se da abordagem das disciplinas relevantes para uma eficiente prestação jurisdicional do pré-sal e do comércio internacional. Em seguida, o Capítulo 3 trata de analisar o conteúdo das disciplinas ministradas nas Escolas de Magistratura Estadual do Rio de Janeiro e Federal da 2ª Região, bem como as provas para ingresso à carreira da magistratura federal e estadual do Rio de Janeiro e Espírito Santo, no que tange à existência de disciplinas como Direito do Petróleo, Gás e Energia, Direito Econômico, Direito Internacional Público e Privado, Exploração & Produção, Direito do Mar, Direito Marítimo, Direito da Navegação Marítima, Direito Portuário, Direito Aduaneiro e Direito Ambiental. Por fim, serão feitas algumas considerações e apresentadas as referências da pesquisa.

Structure, genomic DNA typing, and kinetic characterization of the D allozyme of placental alkaline phosphatase (PLAP/ALPP)

The D allozyme of placental alkaline phosphatase (PLAP) displays enzymatic properties at variance with those of the common PLAP allozymes. We have deduced the amino acid sequence of the PLAP D allele by PCR cloning of its gene, ALPP Two coding substitutions were found in comparison With the cDNA of the common PLAP F allele, i.e., 692C>G and 1352A>G, which translate into a P209R and E429G substitution. A single nucleotide primer extension (SNuPE) assay was developed using PCR primers that enable the amplification of a 1.9 kb PLAP fragment. Extension primers were then used on this PCR fragment to detect the 692C>G and 1352A>G substitution. The SNuPE assay on these two nucleotide substitutions enabled us to distinguish the PLAP F and D alleles from the PLAP S/I alleles. Functional studies on the D allozyme were made possible by constructing and expressing a PLAP D cDNA, i.e., [Arg209, Gly429] PLAP, into wildtype Chinese hamster ovary cells. We determined the k(cat) and K-m, of the PLAP S, F. and D allozymes using the non,physiological substrate p-nitrophenylphosphate at an optimal pH (9.8) as well as two physiological substrates, i.e., pyridoxal-5'-phosphate and inorganic pyrophosphate at physiological pH (7.5). We found that the biochemical properties of the D allozyme of PLAP are significantly different from those of the common PLAP allozymes. These biochemical findings suggest that a suboptimal enzymatic function by the PLAP D allozyme may be the basis for the apparent negative selective pressure of the PLAP D allele. The development of the SNuPE assay will enable us to test the hypothesis that the PLAP D allele is subjected to intrauterine selection by examining genomic DNA from statistically informative population samples. Hum Mutat 19:258-267, 2002. (C) 2002 Wiley-Liss, Inc.

Razão triglicérides/HDL-C e proteína C reativa de alta sensibilidade na avaliação do risco cardiovascular

Introduction: Calculated indexes from laboratory measurements and the determination of plasma markers are commonly used in cardiovascular risk assessment. Objective: To use triglycerides/high-density lipoprotein cholesterol (HDL-C) ratio, estimate of low-density lipoprotein (LDL) particle size and high sensitive C-reactive protein (hs-CRP) levels to the clinical and laboratorial assessment of coronary risk among patients with coronary disease. Material and methods: Sixty patients were chosen accordingly, 36 female (F) (65 ± 9 years of age) and 24 male (M) (55 ± 10 years of age) diagnosed with stable ischemic cardiopathy at Ana Neri Hospital, Salvador, Bahia, Brazil. The investigation was carried out at the Clinical Biochemistry Laboratory of the College of Pharmacy at Federal University of Bahia (UFBA). Blood samples were collected after a 12-hour fast to determine hs-CRP, lipid profile and risk indicators. All data were assessed through Syncron LX®20 Beckman Coulter. The inferential analysis results were considered significant when p ≤ 5%. Data analysis was carried out with GraphPad Prism® (4.03 version) software. Results: Data analysis showed a positive correlation between hs-CRP and triglycerides/HDL-C ratio only among women (r = 0.4; p = 0.0442), n = 36. The analysis of other parameters did not show any significance. Conclusion: The triglycerides/HDL-C ratio and hs-CRP may be used as indicators of increase in cardiovascular risk among women with coronary artery disease (CAD).

A competência em informação na televisão universitária: Reflexões e contribuições iniciais ao contexto brasileiro

The university television station is a communication vehicle that incorporates principles of social structure from the diffusion of culture, knowledge and education dissemination which may lead to the formation of behaviors and opinions instilling critical thought in individuals and promoting the dialogue between society and university by disseminating the knowledge produced in the academic environment. In this way it is pertinent to know and understand the information literacy of professionals working in this context when it comes to content production for university television station. For this initial discussion are addressed the concepts of university television station as well information literacy in order to establish relations between the two thematic mentioned, the relevance of studies in this scenario in relation to the Information Science context.

Gestão do comportamento informacional apoiada na cultura organizacional e em modelos mentais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gestão, mediação e uso da informação

O livro Gestão, mediação e uso da informação vai ao encontro dos estudos teóricos e metodológicos de objetos e fenômenos que envolvem a gestão, a mediação, uso e apropriação da informação em distintos ambientes

Competência em informação na UFPR TV: a inter-relaçâo entre informação, conhecimento e comunicação

Pós-graduação em Ciência da Informação - FFC

El Individuo en el contexto organizacional y su relación con las tecnologías de la información y comunicación

This article presents and discusses the concepts found in the literature about the individual in the context of business organizations. It also addresses the relations between individuals and information and communication technologies within this environment. It presents the data analysis from the application of a questionnaire among the employees of a private company, in which the following elements were analyzed on a scale of importance: technology, information and people.

Cultura y comportamiento informacional en ambientes empresariales y el proceso de inteligencia competitiva

This article presents and discusses the concepts found in the literature about informational culture, informational behavior, information management, knowledge management and competitive intelligence. It focuses the correlation among those elements and shows, from theliterature analysis, the importance of the informational culture for the development of competitive intelligence in organizations.

Serological study of brucellosis and leptospirosis in equines of island Maiandeua (Algodoal) state of Para

The aim of this study was to study the seroepidemiological profile of brucellosis and leptospirosis in horses traction Island Maiandeua, state of Para. In two distinct periods, blood samples were collected from 52 animals of both sexes and different ages (2 to 17 years), totaling 104 samples. For the research of antibodies anti-Brucella abortus were used in rapid agglutination test in plate. In the first harvest, none animal was positive, however in the second harvest there were three animals reactive serum. The research of antibodies anti-Leptospira spp. was performed with the use of the microscopic agglutination test (MAT), the first harvest was 23.07% reacting animals and 15.38% at the second harvest, for one or more Leptospira spp. with titers ranging from 100 to 200. The predominant serotype at first and second harvest was the Autumnalis 40% and 37.5% respectively. According to age, it was observed in group 1 (2 to 7 years) 27.78% and 13.89% respectively in the two samples and the second group (> 7 years) was found 12.50% and 18 75% of reactive serum. The results observed in this study demonstrated that the island of Maiandeua, state of Para, there is the presence of Leptospira spp, with the most frequent serovar autumnalis and possible exposure of animals to brucella smooth, suggesting low risk of infection in the population of horses examined.

Improved protocol for chromatofocusing on the ProteomeLab PF2D

Beckman-Coulter has recently introduced the ProteomeLab PF2D for 2-D liquid separation of protein samples. The system features separation in the first dimension by chromatofocusing, followed by RP chromatography in the second dimension, allowing the analysis of complex proteomics samples. When used by the standard protocol, reproducibility and column life times are limited, making the use of the instrument very costly. We here present an improved protocol for chromatofocusing, which enhances column life by at least fivefold.

The Electrodeposition of Iron-Manganese Alloys

Although the alteration of properties resulting from the alloying of metals in their usual commercial forms has been extensively investigated, the field of electrodeposition of alloys is believed generally not to have received the atten­tion that it merits.