Produção de compostos fenólicos a partir de células imobilizadas do líquen Parmotrema andinum (Müll. Arg.) Hale e avaliação de atividade antimicrobiana

A utilização de metabólitos secundários obtidos de líquens, na indústria farmacêutica, de cosmético, têxtil e de alimentos deve ser criteriosa, visto que a extração e isolamento desses metabólitos requerem uma grande quantidade de biomassa dificilmente renovável, devido ao crescimento lento do líquen. Atualmente, é possível obter substâncias liquênicas tanto por cultivo de tecidos, como por imobilizações celulares e enzimáticas, a partir do talo in natura, utilizando pequena quantidade de material liquênico. Portanto, este trabalho objetiva investigar a produção de compostos fenólicos a partir de células imobilizadas de Parmotrema andinum (Müll. Arg.) Hale utilizando acetato de sódio como precursor da biossíntese dos fenóis. Ensaios de atividade antimicrobiana com extratos orgânicos do talo in natura, eluatos celulares e do ácido lecanórico isolado de P. andinum Hale demonstraram ação contra bactérias Gram-positivas. Através de testes biocromatográficos foi possível associar a atividade antibacteriana ao ácido lecanórico e uma substância não identificada presente na espécie. As substâncias produzidas através de imobilização celular não exibiram ação inibitória frente os microrganismos testados.

Diversidade e sazonalidade de ácaros (Acari) em seringal (Hevea brasiliensis, Muell. Arg.) no Noroeste do Estado de São Paulo

Brazilian southeastern region has soil and climate conditions suitable for the growing of rubber trees, and most part of national yield arises from São Paulo State. The aims proposed for this work were to determine the diversity, the richness and the seasonal occurrence of mites found in a rubber tree crop in a triennial survey with monthly samplings, as well as to estimate the populational density of the major phytophagous species. This study found 74,407 mites from 26 species belonging to 10 families. The phytophagous and predators represented 95.4% and 3.9% of the total abundance, respectively. Twelve species were rare, six accessories and eight constant. The families Phytoseiidae and Tydeidae had the greatest richness (five and four species, respectively). The most numerous species was Calacarus heveae Feres (50,573), with great abundance at the end of rainy season until the beginning of dry season. Among predators, the most abundant were Zetzellia quasagistemas Hernandes & Feres (1,345), Pronematus sp. (455), Zetzellia agistzellia Hernandes & Feres (409) and Euseius citrifolius Denmark & Muma (243). C. heveae had greatest densities on March and April 2003, and Lorryia formosa Cooreman and Tenuipalpus heveae Baker on March and May 2001, respectively. Many stigmaeids were observed in association with colonies of L. formosa preying their eggs and immatures.

Diversidade e sazonalidade de ácaros (Acari) em seringal (Hevea brasiliensis, Muell. Arg.) no Noroeste do Estado de São Paulo

A Região Sudeste do Brasil apresenta aspectos edafo-climáticos favoráveis para o plantio e desenvolvimento da seringueira, sendo o estado de São Paulo o maior produtor nacional de látex. Este trabalho teve como objetivos determinar, através de coletas mensais durante três anos de estudo, a diversidade, a riqueza de espécies e a sua sazonalidade, bem como realizar estimativas de densidade populacional das principais espécies de ácaros presentes nas folhas. Todos os ácaros foram montados em lâminas de microscopia, totalizando 74.407 indivíduos, de 26 espécies pertencentes a 10 famílias. Os fitófagos representaram 95,4% do total de indivíduos coletados e os predadores 3,9%. Doze espécies foram consideradas acidentais, seis foram acessórias e oito constantes. Apresentaram maior número de espécies as famílias Phytoseiidae (cinco) e Tydeidae (quatro). A espécie mais abundante foi Calacarus heveae Feres (50.573), com maior abundância nos meses correspondentes ao término da estação chuvosa e início da estação seca na região. Dentre os predadores, a mais abundante foi Zetzellia quasagistemas Hernandes & Feres (1.345), seguida por Pronematus sp. (455), Zetzellia agistzellia Hernandes & Feres (409) e Euseius citrifolius Denmark & Muma (243). C. heveae apresentou maior densidade populacional em março e abril de 2003, e Lorryia formosa Cooreman e Tenuipalpus heveae Baker em março e maio de 2001, respectivamente. Muitos estigmeídeos foram observados associados a agrupamentos de L. formosa predando seus ovos e estágios imaturos.

Endothelium dependent expression and underlying mechanisms of des-Arg(9)-bradykinin-induced B1R-mediated vasoconstriction in rat portal vein

Endothelial dysfunction has been implicated in portal vein obstruction, a condition responsible for major complications in chronic portal hypertension. Increased vascular tone due to disruption of endothelial function has been associated with an imbalance in the equilibrium between endothelium-derived relaxing and contracting factors. Herein, we assessed underlying mechanisms by which expression of bradykinin B-1 receptor (B1R) is induced in the endothelium and how its stimulation triggers vasoconstriction in the rat portal vein. Prolonged in vitro incubation of portal vein resulted in time- and endothelium-dependent expression of B1R and cyclooxygenase-2 (COX-2). Inhibition of protein kinase C (PKC) or phosphatidylinositol 3-kinase (PI3K) significantly reduced expression of B1R through the regulation of transcription factors, activator protein-1 (AP-1) and cAMP response element-binding protein (CREB). Moreover, pharmacological studies showed that B1R-mediated portal vein contraction was reduced by COX-2, but not COX-1, inhibitors. Notably, activation of endothelial B1R increased phospholipase A(2)/COX-2-derived thromboxane A(2) (TXA(2)) levels, which in turn mediated portal vein contraction through binding to TXA(2) receptors expressed in vascular smooth muscle cells. These results provide novel molecular mechanisms involved in the regulation of B1R expression and identify a critical role for the endothelial B1R in the modulation of portal vein vascular tone. Our study suggests a potential role for B1R antagonists as therapeutic tools for diseases where portal hypertension may be involved. (C) 2012 Elsevier Inc. All rights reserved.

Homozygous Cys542-->Arg substitution in GPIIIa in a Swiss patient with type I Glanzmann's thrombasthenia

Glanzmann's thrombasthenia (GT) arises from a qualitative or quantitative defect in the GPIIb-IIIa complex (integrin alphaIIbbeta3), the mediator of platelet aggregation. We describe a patient in whom clinical and laboratory findings typical of type I GT were found together with a second pathology involving neurological and other complications symptomatic of tuberous sclerosis. Analysis of platelet proteins by Western blotting revealed trace amounts of normally migrating GPIIb and equally small amounts of GPIIIa of slightly slower than normal migration. Flow cytometry confirmed a much decreased binding to platelets of monoclonal antibodies to GPIIb, GPIIIa or GPIIb-IIIa, and an antibody to the alphav subunit also showed decreased binding. Nonradioactive PCR single-strand conformation polymorphism analysis followed by direct sequencing of PCR-amplified DNA fragments showed a homozygous point mutation (T to C) at nucleotide 1722 of GPIIIa cDNA and which led to a Cys542-->Arg substitution in the GPIIIa protein. The mutation gave rise to a HinP1 I restriction site in exon 11 of the GPIIIa gene and allele-specific restriction enzyme analysis of family members confirmed that a single mutated allele was inherited from each parent. This amino acid substitution presumably changes the capacity for disulphide bond formation within the cysteine-rich core region of GPIIIa and its study will provide new information on GPIIb-IIIa and alphavbeta3 structure and biosynthesis.

Evaluation of the transforming growth factor beta1 codon 25 (Arg-->Pro) polymorphism in alcoholic liver disease

INTRODUCTION: Liver cirrhosis develops only in a minority of heavy drinkers. Genetic factors may account for some variation in the progression of fibrosis in alcoholic liver disease (ALD). Transforming growth factor beta 1 (TGFbeta1) is a key profibrogenic cytokine in fibrosis and its gene contains several polymorphic sites. A single nucleotide polymorphism at codon 25 has been suggested to affect fibrosis progression in patients with chronic hepatitis C virus infection, fatty liver disease, and hereditary hemochromatosis. Its contribution to the progression of ALD has not been investigated sufficiently so far. PATIENTS AND METHODS: One-hundred-and-fifty-one heavy drinkers without apparent ALD, 149 individuals with alcoholic cirrhosis, and 220 alcoholic cirrhotics who underwent liver transplantation (LTX) were genotyped for TGFbeta1 codon 25 variants. RESULTS: Univariate analysis suggested that genotypes Arg/Pro or Pro/Pro are associated with decompensated liver cirrhosis requiring LTX. However, after adjusting for patients' age these genotypes did not confer a significant risk for cirrhosis requiring LTX. CONCLUSION: TGFbeta1 codon 25 genotypes Arg/Pro or Pro/Pro are not associated with alcoholic liver cirrhosis. Our study emphasizes the need for adequate statistical methods and accurate study design when evaluating the contribution of genetic variants to the course of chronic liver diseases.

Phenotypic variability in familial combined pituitary hormone deficiency caused by a PROP1 gene mutation resulting in the substitution of Arg-->Cys at codon 120 (R120C).

As pituitary function depends on the integrity of the hypothalamic-pituitary axis, any defect in the development and organogenesis of this gland may account for a form of combined pituitary hormone deficiency (CPHD). A mutation in a novel, tissue-specific, paired-like homeodomain transcription factor, termed Prophet of Pit-1 (PROP1), has been identified as causing the Ames dwarf (df) mouse phenotype, and thereafter, different PROP1 gene alterations have been found in humans with CPHD. We report on the follow-up of two consanguineous families (n = 12), with five subjects affected with CPHD (three males and two females) caused by the same nucleotide C to T transition, resulting in the substitution of Arg-->Cys in PROP1 at codon 120. Importantly, there is a variability of phenotype, even among patients with the same mutation. The age at diagnosis was dependent on the severity of symptoms, ranging from 9 months to 8 yr. Although in one patient TSH deficiency was the first symptom of the disorder, all patients became symptomatic by exhibiting severe growth retardation and failure to thrive, which was mainly caused by GH deficiency (n = 4). The secretion of the pituitary-derived hormones (GH, PRL, TSH, LH, and FSH) declined gradually with age, following a different pattern in each individual; therefore, the deficiencies developed over a variable period of time. All of the subjects entered puberty spontaneously, and the two females also experienced menarche and periods before a replacement therapy was necessary.

Hevea brasiliensis Müll. Arg. im überschwemmungsfreien Gebiet des Amazonenstromes

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Antropología y Desarrollo (Fase II): formas de coordinación e institucionalización de la economía en el sector agrario (Misiones, Arg.) 16H310

Actividades desarrolladas durante el período: revisión bibliográfica; diseño del trabajo de campo; realización de observaciones y entrevistas; análisis de datos; preparación de ponencias.

Lecturas para agricultores. Herramientas intelectuales y representaciones de la agricultura familiar en Misiones (Arg.)

El artículo describe el trabajo simbólico llevado a cabo por por las agencias de desarrollo rural y las organizaciones agrarias en la provincia de Misiones en la década de 1990. Se consideran las publicaciones destinadas a los pequeños productores y las formas de apropiación de los textos a partir de conceptos de la historia cultural. Los impresos analizados ponen en juego distintas representaciones de la agricultura familiar, especialmente las nuevas categorías agrarias que se difunden en la provincia (campesinos, mujeres rurales, agricultores sin tierra). La producción de los textos se organiza de acuerdo a las dimensiones que estructuran el campo del desarrollo rural: saberes nativos versus transferencia tecnológica; integración agroindustrial versus autonomía campesina; agricultura industrial versus agroecología, etc.

Lecturas para agricultores. Herramientas intelectuales y representaciones de la agricultura familiar en Misiones (Arg.)

El artículo describe el trabajo simbólico llevado a cabo por por las agencias de desarrollo rural y las organizaciones agrarias en la provincia de Misiones en la década de 1990. Se consideran las publicaciones destinadas a los pequeños productores y las formas de apropiación de los textos a partir de conceptos de la historia cultural. Los impresos analizados ponen en juego distintas representaciones de la agricultura familiar, especialmente las nuevas categorías agrarias que se difunden en la provincia (campesinos, mujeres rurales, agricultores sin tierra). La producción de los textos se organiza de acuerdo a las dimensiones que estructuran el campo del desarrollo rural: saberes nativos versus transferencia tecnológica; integración agroindustrial versus autonomía campesina; agricultura industrial versus agroecología, etc.

Lecturas para agricultores. Herramientas intelectuales y representaciones de la agricultura familiar en Misiones (Arg.)

El artículo describe el trabajo simbólico llevado a cabo por por las agencias de desarrollo rural y las organizaciones agrarias en la provincia de Misiones en la década de 1990. Se consideran las publicaciones destinadas a los pequeños productores y las formas de apropiación de los textos a partir de conceptos de la historia cultural. Los impresos analizados ponen en juego distintas representaciones de la agricultura familiar, especialmente las nuevas categorías agrarias que se difunden en la provincia (campesinos, mujeres rurales, agricultores sin tierra). La producción de los textos se organiza de acuerdo a las dimensiones que estructuran el campo del desarrollo rural: saberes nativos versus transferencia tecnológica; integración agroindustrial versus autonomía campesina; agricultura industrial versus agroecología, etc.

The primary fibrin polymerization pocket: Three-dimensional structure of a 30-kDa C-terminal γ chain fragment complexed with the peptide Gly-Pro-Arg-Pro

After vascular injury, a cascade of serine protease activations leads to the conversion of the soluble fibrinogen molecule into fibrin. The fibrin monomers then polymerize spontaneously and noncovalently to form a fibrin gel. The primary interaction of this polymerization reaction is between the newly exposed N-terminal Gly-Pro-Arg sequence of the α chain of one fibrin molecule and the C-terminal region of a γ chain of an adjacent fibrin(ogen) molecule. In this report, the polymerization pocket has been identified by determining the crystal structure of a 30-kDa C-terminal fragment of the fibrin(ogen) γ chain complexed with the peptide Gly-Pro-Arg-Pro. This peptide mimics the N terminus of the α chain of fibrin. The conformational change in the protein upon binding the peptide is subtle, with electrostatic interactions primarily mediating the association. This is consistent with biophysical experiments carried out over the last 50 years on this fundamental polymerization reaction.

pH gating of ROMK (Kir1.1) channels: Control by an Arg-Lys-Arg triad disrupted in antenatal Bartter syndrome

Inward-rectifier K+ channels of the ROMK (Kir1.1) subtype are responsible for K+ secretion and control of NaCl absorption in the kidney. A hallmark of these channels is their gating by intracellular pH in the neutral range. Here we show that a lysine residue close to TM1, identified previously as a structural element required for pH-induced gating, is protonated at neutral pH and that this protonation drives pH gating in ROMK and other Kir channels. Such anomalous titration of this lysine residue (Lys-80 in Kir1.1) is accomplished by the tertiary structure of the Kir protein: two arginines in the distant N and C termini of the same subunit (Arg-41 and Arg-311 in Kir1.1) are located in close spatial proximity to the lysine allowing for electrostatic interactions that shift its pKa into the neutral pH range. Structural disturbance of this triad as a result from a number of point mutations found in patients with antenatal Bartter syndrome shifts the pKa of the lysine residue off the neutral pH range and results in channels permanently inactivated under physiological conditions. Thus, the results provide molecular understanding for normal pH gating of Kir channels as well as for the channel defects found in patients with antenatal Bartter syndrome.