Conservação de mamão 'Formosa' minimamente processado armazenado sob refrigeração

O objetivo deste trabalho foi avaliar a qualidade de produtos minimamente processados de mamão 'Formosa', fatias ou metades, armazenados sob diferentes temperaturas (3ºC, 6ºC e 9ºC). Utilizou-se de frutos que, depois de selecionados quanto ao grau de maturação e ausência de danos, foram lavados, desinfeccionados com cloro (200 mg.L-1) e armazenados a 12ºC, por 12 horas antes do processamento, que foi feito manualmente, a 12ºC. Os mamões, depois de descascados, foram cortados em fatias (5,0 x 2,5 cm) ou em metades longitudinais sem as pontas, que, depois de enxaguadas com água sanitizada (20 mg de cloro.L-1) e escorridas por 2-3 minutos, foram embaladas em bandejas de isopor recobertas com filme de PVC esticável (metades) ou em bandejas de tereftalato de polietileno - PET (fatias) e imediatamente armazenadas sob refrigeração. A avaliação destes produtos foi feita a cada 3 dias, quanto à resistência da polpa, coloração, pH e conteúdos de sólidos solúveis, acidez titulável, ácido ascórbico e de carboidratos, solúveis e de redutores. Durante o armazenamento, as fatias tenderam a se tornarem mais firmes, com a sua polpa apresentando pequeno escurecimento. Os conteúdos de carboidratos solúveis e de redutores e de sólidos solúveis não foram afetados pelo tipo de corte, temperatura de armazenamento ou embalagem. Durante o armazenamento, os teores de acidez titulável aumentaram nas fatias e diminuíram nas metades e observou-se influência da temperatura. Não se observaram reduções nos teores de ácido ascórbico durante o armazenamento, ou influência dos cortes ou das embalagens. Os produtos mantiveram-se adequados para comercialização até o 10º dia de armazenamento.

Expression of fibroblast growth factor-8 and its cognate receptors, fibroblast growth factor receptor (FGFR)-3c and-4, in fetal bovine preantral follicles

Paracrine cell signaling is thought to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family have been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR-3c and FGFR-4) are expressed in bovine preantral follicles. Reverse transcription-polymerase chain reaction was used to amplify bovine FGF-8, FGFR-3c, and FGFR-4 from preantral follicle samples and a variety of fetal and adult tissues. All three genes were widely expressed in fetal tissues, with a restricted expression pattern in adult tissues. FGF-8 and FGFR-3c were expressed in secondary follicles in 70% of fetuses examined, whereas FGFR-4 expression was significantly less frequent (20%). FGFR-3c expression frequency was significantly lower in primordial compared to secondary follicles, and FGF-8 expression showed a similar trend. FGFR-4 was only observed when all follicle classes of an individual were expressing both FGF-8 and FGFR-3c. We conclude that FGF-8 and its receptors are expressed in preantral follicles in a developmentally regulated manner. (C) 2005 Wiley-Liss, Inc.

Expression of fibroblast growth factor-8 and regulation of cognate receptors, fibroblast growth factor receptor-3c and-4, in bovine antral follicles

Paracrine cell signaling is believed to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family has been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR3c and FGFR4) are expressed in bovine antral follicles. RT-PCR was used to analyze bovine Fgf8, Fgfr3c and Fgfr4 mRNA levels in oocytes, and granulosa and theca cells. Fgf8 expression was detected in oocytes and in granulosa and theca cells; this expression pattern differs from that reported in rodents. Granulosa and theca cells, but not oocytes, expressed Fgfr3c, and expression in granulosa cells increased significantly with follicle estradiol content, a major indicator of follicle health. Fgfr4 expression was restricted to theca cells in the follicle, and decreased significantly with increasing follicle size. To investigate the potential regulation of Fgfr3c expression in the bovine granulosa, cells were cultured in serum-free medium with FSH or IGF-I; gene expression was upregulated by FSH but not by IGF-I. The FSH-responsive and developmentally regulated patterns of Fgfr3c mRNA expression suggest that this receptor is a potential mediator of paracrine signaling to granulosa cells during antral follicle growth in cattle.

Regulação da expressão dos receptores de fatores de crescimento fibroblásticos 3c e 4 (FGFR-3c e FGFR-4) em folículos antrais bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Martha Thomas Fitzgerald Papers - Accession 273

The Martha Thomas Fitzgerald Papers consist of biographical data, correspondence, minutes, newspaper clippings, memoranda, reports, and photographs (of particular interest are the many photographs of rural S.C. school houses in the 1920s). The collection pertains to Mrs. Fitzgerald’s work with the South Carolina Department of Education, the South Carolina House of Representatives, and her work with various civic organizations such as the Altrusa Club, the League of Women Voters, the Daughters of American Colonists, United Daughters of the Confederacy (UDC), Business and Professional Women’s Club (BPW), Delta Kappa Gamma, South Carolina Vocational Rehabilitation, and the Salvation Army. There is also information on the Status of Women Commission Council on Aging, agriculture, Queens College, University of South Carolina, Winthrop University, Columbia University, public health, South Carolina history, City of Columbia, South Carolina, and Richland County, South Carolina. Correspondents include Strom Thurmond and three letters from John F. Kennedy when he was senator. Mrs. Fitzgerald was the first woman elected to the South Carolina House of Representatives in a general election.

The 3C cooperation model applied to the classical requirement analysis

Aspects related to the users' cooperative work are not considered in the traditional approach of software engineering, since the user is viewed independently of his/her workplace environment or group, with the individual model generalized to the study of collective behavior of all users. This work proposes a process for software requirements to address issues involving cooperative work in information systems that provide distributed coordination in the users' actions and the communication among them occurs indirectly through the data entered while using the software. To achieve this goal, this research uses ergonomics, the 3C cooperation model, awareness and software engineering concepts. Action-research is used as a research methodology applied in three cycles during the development of a corporate workflow system in a technological research company. This article discusses the third cycle, which corresponds to the process that deals with the refinement of the cooperative work requirements with the software in actual use in the workplace, where the inclusion of a computer system changes the users' workplace, from the face to face interaction to the interaction mediated by the software. The results showed that the highest degree of users' awareness about their activities and other system users contribute to a decrease in their errors and in the inappropriate use of the system.

Subcellular Localization of the Non-Structural Proteins 3C and 3CD of the Honeybee Virus Deformed Wing Virus

Apis mellifera L., the European honeybee, is a crucial pollinator of many important agricultural crops in the United States. Recently, honeybee colonies have been affected by Colony Collapse Disorder (CCD), a disorder in which the colony fails due to the disappearance of a key functional group of worker bees. Though no direct causalrelationship has been confirmed, hives that experience CCD have been shown to have a high incidence of Deformed Wing Virus (DWV), a common honeybee virus. While the genome sequence and gene-order of DWV has been analyzed fairly recently, few other studies have been performed to understand the molecular characterization of the virus.Since little is known about where DWV proteins localize in infected host cells, the objective of this project was to determine the subcellular localization of two of the important non-structural proteins that are encoded in the DWV genome. This project focused on the protein 3C, an autocatalytic protease which cleaves itself from a longer polyprotein and helps to cut all of the other proteins apart from one another so that they can become functional, and 3D, the RNA-dependent RNA polymerase (RdRp) which is critical for replication of the virus because it copies the viral genome. By tagging nested constructs containing these two proteins and tracking where they localized in living cells, this study aimed to better understand the replication of DWV and to elicit possible targetsfor further research on how to control the virus. Since DWV is a picorna-like virus, distantly related to human viruses such as polio, and picornavirus non-structural proteins aggregate at cellular membranes during viral replication, the major hypothesis was that the 3C and 3CD proteins would localize at cellular organelle membranes as well. Using confocal microscopy, both proteins were found to localize in the cytoplasm, but the 3CDprotein was found to be mostly diffuse cytoplasmic, and the 3C protein was found to localize more specifically on membranous structures just outside of the nucleus.

Implant Failure Predictors in the Posterior Maxilla: A Retrospective Study of 273 Consecutive Implants

Background: The goal of this study was to retrospectively analyze a cohort of 136 patients who underwent dental implant placement in the posterior maxilla at the University of Connecticut Health Center to assess and identify predictors for implant failure in the posterior maxilla. Methods: Data were retrieved from patient charts to identify subjects older than 21 years of age who received dental implant(s) in the posterior maxilla. Patients without a postoperative baseline radiograph were excluded. A recall radiograph was taken 3 to 6 months after implant placement. If there was no recall radiograph, the subject was contacted for a recall visit that included a clinical evaluation and radiographs to determine the implant status. Based on a univariate screening, variables considered potential implant failure predictors included gender, diabetes, smoking, implant length, implant diameter, membrane use, sinus-elevation technique, and surgical complications. These parameters were further assessed, and a multivariable logistic regression was performed with implant failure as a dependant variable. All tests of significance were evaluated at the 0.05 error level. Results: Two hundred seventy-three implants were placed in the posterior maxilla. Fourteen implants failed (early and late failures combined), resulting in a 94.9% overall survival rate. The survival rates for the sinus-elevation group and native bone group were 92.2% and 96.7%, respectively (P = 0.090). Based on the multivariable analysis, sinus floor-elevation procedures were not associated with increased risk for implant failure (P = 0.702). In contrast, smoking and surgical complications had a statistically significant effect on implant failure; the odds ratios for implant failure were 6.4 (P = 0.025) and 8.2 (P = 0.004), respectively. Conclusion: Sinus-elevation procedures with simultaneous or staged implant placement do not increase the risk for implant failure, whereas smoking and surgical complications markedly increase the risk for implant failure.

Ms.Ff.F.Stoltze 3. 273 - Brief von Ernst Schalck an Friedrich Stoltze

Umzug, Frankfurter Latern

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 273 - Bitte an Jules Lunteschütz um die Dankesmitteilung an Arthur Schopenhauer wegen dessen eingetroffenen Briefes

u.a.: Unfall Schopenhauers; Arthur Schopenhauer;

Ms. var. oct. 8 No. 3c - Über die Duchoborzen. Erstes Heft

Die im Katalog angegebene Blattzahl weicht von der vorhandenen ab.