The occurrence of intra-operative hypotension varies between hospitals: observational analysis of more than 147,000 anaesthesia.

BACKGROUND: Hypotension, a common intra-operative incident, bears an important potential for morbidity. It is most often manageable and sometimes preventable, which renders its study important. Therefore, we aimed at examining hospital variations in the occurrence of intra-operative hypotension and its predictors. As secondary endpoints, we determined to what extent hypotension relates to the risk of post-operative incidents and death. METHODS: We used the Anaesthesia Databank Switzerland, built on routinely and prospectively collected data on all anaesthesias in 21 hospitals. The three outcomes were assessed using multi-level logistic regression models. RESULTS: Among 147,573 anaesthesias, hypotension ranged from 0.6% to 5.2% in participating hospitals, and from 0.3% up to 12% in different surgical specialties. Most (73.4%) were minor single events. Age, ASA status, combined general and regional anaesthesia techniques, duration of surgery and hospitalization were significantly associated with hypotension. Although significantly associated, the emergency status of the surgery had a weaker effect. Hospitals' odds ratios for hypotension varied between 0.12 and 2.50 (P < or = 0.001), even after adjusting for patient and anaesthesia factors, and for type of surgery. At least one post-operative incident occurred in 9.7% of the procedures, including 0.03% deaths. Intra-operative hypotension was associated with a higher risk of post-operative incidents and death. CONCLUSION: Wide variations remain in the occurrence of hypotension among hospitals after adjustment for risk factors. Although differential reporting from hospitals may exist, variations in anaesthesia techniques and blood pressure maintenance may also have contributed. Intra-operative hypotension is associated with morbidities and sometimes death, and constant vigilance must thus be advocated.

Draft genome sequence of Bacillus thuringiensis 147, a Brazilian strain with high insecticidal activity

Bacillus thuringiensisis a ubiquitous Gram-positive and sporulating bacterium. Its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. We report here the draft genome sequence ofB. thuringiensis147, a strain isolated from Brazil and with high insecticidal activity. The assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). We identified 12 coding regions, located in two plasmids, which encode insecticidal proteins.

Atividades de nitrogenase e redutase de nitrato e produtividade do feijoeiro “Ouro Negro” em resposta à adubação foliar com molibdênio

A efetividade do suprimento de nitrogênio ao feijoeiro, por meio de fertilizante e, ou, de fixação biológica, e a dependência desses processos à disponibilidade de molibdênio constituem, ainda, um problema mal resolvido. Objetivando avaliar os efeitos da aplicação foliar de Mo na atividade das enzimas nitrogenase e redutase do nitrato e na produtividade do feijoeiro, realizou-se um experimento, em condições de campo, em um Podzólico Vermelho-Amarelo do município de Coimbra (MG). Os tratamentos constituíram-se de doses crescentes de Mo (0, 40, 80 e 120 g ha-1 de Mo), aplicadas em adubação foliar aos 25 dias da emergência. Na adubação de base, usaram-se 20 kg ha-1 de N e 60 kg ha-1 de K2O. Não foi aplicado nitrogênio em cobertura. A aplicação foliar de Mo aumentou as atividades da nitrogenase e da redutase do nitrato, mantendo-as em patamares mais altos durante o ciclo da cultura, proporcionando maiores teores de N nas folhas e maior produtividade. A eficiência máxima foi alcançada com 80 g ha-1 de Mo, com produtividade de 1.893 kg ha-1 de grãos, 3,23 vezes maior que a da testemunha (sem Mo). A aplicação foliar de Mo aumentou, também, em 1,54 vez o número de vagens por planta, em 0,23 o número de grãos por vagem e em 0,15 o peso de 100 grãos, em comparação ao tratamento que não recebeu Mo.

Friday Facts, July 20, 2012, Vol. 147

Bureau of Nutrition and Health Promotion part of the Iowa Department of Public Health produces of weekly newsletter about the Iowa WIC Program for the State of Iowa citizen.

Guidelines for the Inclusion of Left-Turn Lanes at Rural Highway Intersections, HR-147, 1970

Provision of left turn lanes is a major problem which lacks an objective approach. Various techniques and procedures in use have been reviewed. Traffic characteristics at typical Iowa intersections have been measured. A rational approach for inclusion of a left turn lane has been developed, based on relating the benefits to the road user to the cost of providing the added turing lane. An analysis of field data gathered under this project indicates that the use of theoretical distribution to describe vehicle headways is not applicable to rural Iowa two lane roads. As an alternate approach the mass of field data gathered were examined using multiple regression techniques to yield equations for predicting stops and delays. The benefit-cost ratio technique is recommended as the criterion for decision making.

Resolución N° 147 ¿Cuál es la fuente de consulta primaria cuando se verifica en las sociedades comerciales el requisito de la convocatoria?

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Phosphorylation du CTD de l'ARN polymérase II et impact de l'histone H2A.Z sur le positionnement des nucléosomes chez S. cerevisiae

La phosphorylation du domaine C-terminal de l’ARN polymérase II permet à ce complexe protéique d’exécuter la transcription des gènes, en plus de coupler à la transcription des événements moléculaires comme la maturation des ARNm. Mes résultats montrent que même si cette phosphorylation suit un patron similaire à l’ensemble des gènes, il existe des exceptions pouvant être dues à des mécanismes alternatifs de phosphorylation du CTD. Le présent ouvrage s’intéresse également au rôle qu’occupe la variante d’histone H2A.Z dans l’organisation de la chromatine. Des études précédentes on montré que le positionnement de certains nucléosomes le long de l’ADN serait influencé par H2A.Z et aurait une influence sur la capacité de transcrire les gènes. Par une approche génomique utilisant les puces à ADN, j’ai cartographié l’impact de la délétion de H2A.Z sur la structure des nucléosomes. Enfin, des résultats intéressants sur la dynamique d’incorporation de H2A.Z à la chromatine ont été obtenus.