噬菌体展示随机十肽库的构建及抗WSSV单链抗体A1的模拟抗原表位的淘选和鉴定

本实验以pCANTAB5E噬菌粒为载体,成功构建了较高容量的噬菌体展示随机十肽库,并将其应用于抗原模拟表位的淘选和鉴定。将一种特异识别对虾白斑综合症病毒(Whitespotsyndromevirus,WSSV)的单链抗体A1对十肽库和十五肽库分别进行淘选,结果得到一系列能与单链抗体A1特异性结合的阳性克隆。将这些阳性克隆所编码的多肽氨基酸序列与已知的单链抗体A1的抗原WSSV388片段氨基酸序列做比对,发现多数阳性多肽序列都与WSSV388片段序列的C端一处K????R??R?QS的氨基酸片段相似,由此推

HTGR fuel element depletion benchmark: Stage three results

Recently, a new numerical benchmark exercise for High Temperature Gas Cooled Reactor (HTGR) fuel depletion was defined. The purpose of this benchmark is to provide a comparison basis for different codes and methods applied to the burnup analysis of HTGRs. The benchmark specifications include three different models: (1) an infinite lattice of tristructural isotropic (TRISO) fuel particles, (2) an infinite lattice of fuel pebbles, and (3) a prismatic fuel including fuel and coolant channels. In this paper, we present the results of the third stage of the benchmark obtained with MCNP based depletion code BGCore and deterministic lattice code HELIOS 1.9. The depletion calculations were performed for three-dimensional model of prismatic fuel with explicitly described TRISO particles as well as for two-dimensional model, in which double heterogeneity of the TRISO particles was eliminated using reactivity equivalent physical transformation (RPT). Generally, good agreement in the results of the calculations obtained using different methods and codes was observed.

Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus)

Natural resistance associated macrophage protein (Nramp) controls partially innate resistance to intracellular parasites. Its function is to enhance the ability of macrophages to kill pathogens. However, little is known about the structure and function of Nramp in lower vertebrates such as teleosts. We have recently isolated a cDNA encoding Nramp from Japanese flounder (Paratichthys olivaceus). The full-length cDNA of the Nramp is 3066 bp in length, including 224 bp 5' terminal UTR, 1662 bp encoding region and 1180 bp 3' terminal UTR. The 1662-nt open reading frame was found to code for a protein with 554 amino acid residues. Comparison of amino acid sequence indicated that Japanese flounder Nramp consists of 12 transmembrane (TM) domains. A consensus transport motif (CTM) containing 20 residues was observed between transmembrane domains 8 and 9. The deduced amino acid sequence of Japanese flounder had 77.30%, 82.71%, 82.67%, 79.64%, 80.72%, 90.97%, 91.16%, 60.14%, 71.48%, 61.69%, 72.37% identity with that of rainbow trout Nramp alpha and beta, channel catfish Nramp, fathead minnow Nramp, common carp Nramp, striped sea bass Nramp, red sea bream Nramp, mouse Nramp 1 and 2, human Nramp 1 and 2, respectively. RT-PCR indicated that Nramp transcripts were highly abundant in spleen, head kidney, abundant in intestine, liver and gill, and less abundant in heart. The level of Nramp mRNA in embryos gradually increases during embryogenesis from 4 h (8 cell stage) to 80 h (hatched stage) after fertilization. (c) 2005 Elsevier Ltd. All rights reserved.

A passive acoustic monitoring method applied to observation and group size estimation of finless porpoises

The present study aimed at determining the detection capabilities of an acoustic observation system to recognize porpoises under local riverine conditions and compare the results with sighting observations. Arrays of three to five acoustic data loggers were stationed across the main channel of the Tian-e-zhou Oxbow of China's Yangtze River at intervals of 100-150 m to record sonar. signals of free-ranging finless porpoises (Neophocaena phocaenoides). Acoustic observations, concurrent with visual observations, were conducted at two occasions on 20-22 October 2003 and 17-19 October 2004. During a total of 42 h of observation, 316 finless porpoises were sighted and 7041 sonar signals were recorded by loggers. The acoustic data loggers recorded ultrasonic signals of porpoises clearly, and detected the presence of porpoises with a correct detection level of 77.6% and a false alarm level of 5.8% within an effective distance of 150 m. Results indicated that the stationed passive acoustic observation method was effective in detecting the presence of porpoises and showed potential in estimating the group size. A positive linear correlation between the number of recorded signals and the group size of sighted porpoises was indicated, although it is faced with some uncertainty and requires further investigation. (C) 2005 Acoustical Society of America.

Quantum efficiency and temperature coefficients of GaInP/GaAs dual-junction solar cell

GaInP/GaAs dual-junction solar cell with a conversion efficiency of 25.2% has been fabricated using metalorganic chemical vapor deposition (MOCVD) technique. Quantum efficiencies of the solar cell were measured within a temperature range from 25 to 160A degrees C. The results indicate that the quantum efficiencies of the subcells increase slightly with the increasing temperature. And red-shift phenomena of absorption limit for all subcells are observed by increasing the cell's work temperature, which are consistent with the viewpoint of energy gap narrowing effect. The short-circuit current density temperature coefficients dJ (sc)/dT of GaInP subcell and GaAs subcell are determined to be 8.9 and 7.4 mu A/cm(2)/A degrees C from the quantum efficiency data, respectively. And the open-circuit cell voltage temperature coefficients dV (oc)/dT calculated based on a theoretical equation are -2.4 mV/A degrees C and -2.1 mV/A degrees C for GaInP subcell and GaAs subcell.

PROTON-IMPLANTED NEW TYPE SILICON MATERIAL SUBJECTED TO 2-STEP FURNACE ANNEALING

A high-resistivity defect layer buried beneath the silicon surface layer by using proton implantation and two-step conventional furnace annealing is described. During the first annealing step (600-degrees-C), implanted hydrogen atoms move towards the damage region and then coalesce into hydrogen gas bubbles at the residual defect layer. During the second annealing step (1180-degrees-C) these bubbles do not move due to their large volume. Structural defects are formed around the bubbles at a depth of approximately 0.5-mu-m. The defect layer results in a high resistivity value. Experiments show that the quality of the surface layer has been improved because the surface Hall mobility increased by 20%. The sample was investigated by transmission electron microscopy.

Columnar aerosol optical properties at AERONET sites in central eastern Asia and aerosol transport to the tropical mid-Pacific

IEECAS SKLLQG

~(85)Zr的高自旋态和磁转动带研究

<正>质量数80区的过渡性核表现了集体性和单粒子性的能级结构。对质子数Z为37～40、中子数N=45的原子核,如81Kr,87Mo核,在中低自旋时显示了单粒子特性,而在高自旋态时表现出较多的集体性。 近些年来,在过渡区在束γ谱学,如83Rb、83Y等核研究中,观察到了一串建立在较高K态的增强的△I=1的M1跃迁。它被认为是一种新的激发模式,叫做磁转动带。我们对85Zr的研究目的,一是将其能级推到更高自旋,另一个是寻找该核的磁转动带。

Hadron physics programs at HIRFL-CSRm: Plan and status

An internal target experiment at HIRFL-CSRm is planned for hadron physics, which focuses on hadron spectroscopy, polarized strangeness production and medium effect. A conceptual design of Hadron Physics Lanzhou Spectrometer (HPLUS) is discussed. Related computing framework involves event generation, simulation, reconstruction and final analysis. The R&D works on internal target facilities and sub-detectors are presented briefly.

辽西地区水分亏缺与树木生长关系

利用修正的Thornthwaite最大蒸发力公式,计算了辽西地区不同市的水分亏缺量。各市最大潜在蒸发散计算结果是:朝阳1180.6 mm,锦州1146.0 mm,葫芦岛1140.1 mm,阜新1030.3 mm;水分亏缺量计算结果为:朝阳-658.3 mm,锦州-538.4 mill,葫芦岛-544.8 mm,阜新-626.9 mm。利用美国spss数理统计软件计算了油松与蒙古栎的胸径连年生长量和对应的各年1—12月份的月均温、月降雨量、平均相对湿度的回归关系,表明油松生长与温度紧密,蒙古栎生长与水分关系紧密。

黄土高原森林枯枝落叶层保持水土的有效性

采用人工模拟与天然降水相结合的方法 ,定量研究了枯枝落叶层保持水土的有效性。研究结果表明 ,在黄土高原常见坡度 2 5°和暴雨雨强 1～ 2 mm/m in的条件下 ,该地区油松和山杨林枯枝落叶层保持水土的有效厚度为 0 .9～ 1.0 cm,低于这个数量 ,将明显降低其蓄水减沙效益