Polymorphic microsatellites in largemouth bronze gudgeon (Coreius guichenoti) developed from repeat-enriched libraries and cross-species amplifications

Largemouth bronze gudgeon (Coreius guichenoti) is a medium-sized fish endemic from the upper Yangtze River of China and its survival is threatened by the construction of the Three Gorges Dam. This study reports 20 new polymorphic microsatellites from a repeat-enriched genomic library with a mean number allele of 5.2, and observed and expected heterozygosities ranging from 0.035 to 1, and from 0.13 to 0.917, respectively. In a cross-species amplification test, nine of the 37 tested loci were found to be also polymorphic in a congeneric species, brass gudgeon (C. heterodon). In addition, other four loci from common carp (Cyprinus carpio) were also polymorphic in C. guichenoti. Out of these 24 polymorphic microsatellites, only three loci significantly deviated from Hardy-Weinberg equilibrium in the sampled population (P < 0.0025), and all pairwise tests for linkage disequilibrium among loci were nonsignificant after applying sequential Bonferroni correction (P > 0.0026). These novel microsatellites provide sufficient levels of polymorphism for studies on population genetics and conservation in C. guichenoti and its related species.

外加电场下垂直腔面发射激光器的偏振转换电流（英文）

计算了作用在垂直腔面发射激光器(VCSEL)的上面的分布喇格反射镜(DBR)上的外加电场产生的双折射，并在此基础上通过对自旋反转模型(SFM)的修正，得到面发射激光器在外加电场存在时的偏振转换电流。

Rotamers of m-aminophenol cation studied by mass analyzed threshold ionization spectroscopy and theoretical calculations

The vibrationally resolved spectra of selected rotamers of m-aminophenol have been recorded by mass analyzed threshold ionization spectroscopy in connection with two-color resonant two-photon excitation scheme. The adiabatic ionization energies of the cis and trans rotamers are 61460 +/- 5 and 61734 +/- 5 cm(-1), respectively. The frequencies of modes 1 (breathing) and 18a (in-plane CH bending) are measured to be 744 and 1097 cm(-1) for the cis, and 736 and 1104 cm(-1) for the trans rotamer, respectively. This indicates that different orientation of the OH with respect to the NH2 substituent only slightly influences these two modes. (C) 2004 Elsevier B.V. All rights reserved.

黄土高原地区农田土壤水分高效调控技术

分析黄土高原节水农业的必要性 ,总结出黄土高原农业调水技术主要有 7大类 :培肥地力以肥调水 ,轮作倒茬调水 ,间隙休闲调水 ,抗旱育种调水 ,节水灌溉调水 ,径流农业调水 ,化学技术调水 ;指出在干旱、半干旱的黄土高原地区 ,利用农业调水技术是粮食增产增收的有效措施和必然趋势

小柏根和白石清热冲剂废渣栽培平菇试验

小柏根和白石清热冲剂废渣栽培平菇试验姚志红（中国科学院沈阳应用生态所，１１００１５）随着食用菌栽培业的迅速发展，棉子皮等一些传统生产原料供不应求或价格过高的问题日趋突出，在非产棉地区更成了生产发展的限制因素。因此，不断挖掘当地可利用的潜在资源，开辟新...

Effect of Copolymerization Time on the Microstructure and Properties of Polypropylene/Poly(ethylene-co-propylene) In-Reactor Alloys

Three Polypropylene/Poly(ethylene-co-propylene) (PP/EPR) in-reactor alloys produced by a two-stage slurry/gas polymerization had different ethylene contents and mechanical properties, which were achieved by controlling the copolymerization time. The three alloys were fractionated into five fractions via temperature rising dissolution fractionation (TRDF), respectively. The chain structures of the whole samples and their fractions were analyzed using high-temperature gel permeation chromatography (GPC), Fourier transform infrared (FT-IR), C-13 nuclear magnetic resonance (C-13 NMR), and differential scanning calorimetry (DSC) techniques. These three in-reactor alloys mainly contained four portions: ethylenepropylene random copolymer (EPR), ethylene-propylene (EP) segmented and block copolymers, and propylene homopolymer. The increased copolymerization time caused the increased ethylene content of the sample. The weight percent of EPR, EP segmented and block copolymer also became higher.

近红外光谱测定溶液中甲醇的含量

测量了含微量甲醇(体积分数为0.04%～0.24%)的系列乙醇水溶液的近红外光谱,利用近红外光谱分析建立了预测甲醇含量的定量分析模型。比较了用外部检验法(Test Set-Validation)和交叉检验法(Cross-Validaton)建立的数学模型,研究了使用外部检验法时,校正集和检验集样品数的改变对模型预测结果的影响。结果发现,当校正集样品数为15检验集样品数为6(总样品数为21)时,使用外部检验法建立的数学模型预测结果较好,其校正集的均方根误差和检验集的预测均方根误差(分别为RMSEE和RMSEP)均较小(分别为0.0115和0.0105),而且很接近。结果表明,近红外光谱方法简单,准确而且实用。

Synthesis of gold nanoplates by aspartate reduction of gold chloride

Single crystal nanoplates with thickness less than 30 nm, characterized by hexagonal and truncated triangular shapes bounded mainly by {111} facets, were obtained in large quantities by aspartate reduction of gold chloride.

铁氰化钐修饰电极的制备、表征及电催化

用电化学循环扫描的方法将铁氰化钐沉积在玻碳电极表面,形成铁氰化钐修饰电极(SmHCF/GC),扫描电镜研究表明,SmHCF颗粒(113±13)μm附着在电极表面;红外光谱结果表明,SmCHF中CN的弯曲振动吸收峰出现在20615cm-1处。SmHCF/GC在循环伏安曲线上出现一对很好的氧化还原峰,在20mV/s时,氧化还原峰电位分别为Epa=207mV,Epc=171mV;式量电位E0′=189mV;随着支持电解质浓度的增加,氧化还原峰电位正移,式量电位E0′与支持电解质浓度(logc)之间有线性关系,斜率为504mV。SmHCF/GC电极对神经递质多巴胺(DA)的氧化具有电催化作用,催化电流随DA浓度的增加而增加,在10～150mmol/L范围内有线性关系。

黄连中小檗碱及其同分异构体的电喷雾质谱研究

利用电喷雾多级串联质谱对黄连经水提、醇沉、薄层色谱分离后 ,得到的组分进行了详细研究 ,并对其中小檗碱及其同分异构体表小檗碱的分子离子 [M+ ]进行了源内碰撞诱导解离 (CID) ,在不同源内CID能量(2 5 % ,5 0 % )下碰撞诱解离时存在区别 ,并提出其碎裂机理方面的解释