On the identification of the early developmental stages of clupeiform fishes from Indian waters

Among about 65 species of clupeiform fishes present in the seas around India, most are distributed along both the east and west coasts and have similar spawning seasons and spawning grounds. The most difficult problem experienced with regard to the identification of their early developmental stages is the overlapping sets of diagnostic features among many species within the same genus as well as among species belonging to two or more genera. From studies carried out recently, the present paper sites a few instances wherein certain subtle characters have been used for distinguishing the early developmental stages of a few allied species.

Early and better maturity in carp brood stock with control of environment and feeding

Available of carp breeders in their prim state of maturity is a major constraint in hypophysation. Experiments conducted in a fish farm at Naihati, West Bengal, for two consecutive years, 1983-84 and 1984-85, clearly prove that by manipulation of environmental parameters such as metabolites, dissolved oxygen, running water conditions, as also of stock densities and quality and quantity of feed. Catla catla, Hypophythalmichthys molitrix, Labeo rohita, Cirrhina mrigala and Ctenopharyngodon idella can be made to attain better maturity and spawning stage much earlier than normal i.e. even in summer months and the entire stock spawned during the period from March to September. Percentage of successful breeding, quantities of eggs released and fertilised in relation to the body weight of all the species, were also found to be more in comparison to the brood stock raised through the conventional methods.

Value roadmapping: A structured approach for early stage technology investment decisions

Deciding to invest in early stage technologies is one of the most important tasks of technology management and arguably also the most uncertain. It assumes a particular significance in the rise of technology companies in emerging economies, which have to make appropriate investment decisions. Technology managers already have a wide range of methods and tools at their disposal, but these are mostly focussed on quantitative measures such as discounted cash flow and real options techniques. However, in the early stages of technology development there seems to be a lot of dissatisfaction with these techniques as there appears to be a lack of accuracy with respect to the underlying assumptions that these models require. In order to complement these models this paper will discuss an alternative approach that we call value road-mapping. By adapting roadmapping techniques the potential value streams of early stages technologies can be plotted and hence a clearer consensus based picture of the future potential of new technologies emerges. Roadmapping is a workshop-based process bringing together multifunctional perspectives, and supporting communication in particular between technical and commercial groups. The study is work in progress and is based on a growing number of cases. (c) 2006 PICMET.

Global distribution of Y-chromosome haplogroup C reveals the prehistoric migration routes of African exodus and early settlement in East Asia

The regional distribution of an ancient Y-chromosome haplogroup C-M130 (Hg C) in Asia provides an ideal tool of dissecting prehistoric migration events. We identified 465 Hg C individuals out of 4284 males from 140 East and Southeast Asian populations. We genotyped these Hg C individuals using 12 Y-chromosome biallelic markers and 8 commonly used Y-short tandem repeats (Y-STRs), and performed phylogeographic analysis in combination with the published data. The results show that most of the Hg C subhaplogroups have distinct geographical distribution and have undergone long-time isolation, although Hg C individuals are distributed widely across Eurasia. Furthermore, a general south-to-north and east-to-west cline of Y-STR diversity is observed with the highest diversity in Southeast Asia. The phylogeographic distribution pattern of Hg C supports a single coastal 'Out-of-Africa' route by way of the Indian subcontinent, which eventually led to the early settlement of modern humans in mainland Southeast Asia. The northward expansion of Hg C in East Asia started similar to 40 thousand of years ago (KYA) along the coastline of mainland China and reached Siberia similar to 15 KYA and finally made its way to the Americas. Journal of Human Genetics (2010) 55, 428-435; doi:10.1038/jhg.2010.40; published online 7 May 2010

Early developmental stages of two Secutor species (Family: Leiognathidae) collected from the Bak-khali river estuary of the Bay of Bengal, Bangladesh

Early developmental stages of two Secutor species, Secutor insidiator (Bloch) (11.9-36.0 mm standard length, SL) and Secutor ruconius (Hamilton-Buchanan) (14.0-33.0 mm SL) collected by ichthyoplankton net from the Bak-khali river estuary of the Bay of Bengal, Cox's Bazar, Bangladesh are described and illustrated. All of the fins with supporting spines and rays were present in the smallest collected sizes of both species. With growth of the specimens, significant changes in melanophore patterns were found. S. insidiator is similar to S. ruconius in having upward protracting mouth parts and body colouration, but can be distinguished easily by its more elongate body shape (body depth 38-47% of SL compared with 46-52% of SL in Secutor ruconius). Both the species occurred round the year from August 1998 to July 1999. The surface water temperature and salinity during the study period varied from 22.0-32) C and 10-37 ppt respectively.

Early developmental stages of Nandus nandus (Ham.)

In order to study the early developmental stages of Nandus nandus an experiment was conducted, where eggs and milt were obtained from the laboratory reared N nandus by stripping after 15 hours of 150 mg/kg body weight of carp PG extract injection. Then the eggs were fertilized in the laboratory and subsequent developmental stages were studied. First cleavage (two cell), four cell, eight cell, sixteen cell and multi cell stages were found 30, 50, 70, 105 and 160 minutes after fertilization respectively. Morula, early gastrula, middle gastrula, late gastrula and yolk plug stages were found 5, 8, 9, 11 and 13 hours after fertilization respectively. Hatching occurred within 20±2 hours after fertilization, and larvae were measured 1.60 mm in diameter. After one hour of hatching two melanophore bands were found at the caudal region of the body of the larvae. Eyes were first observed in l 0 hours, pectoral and pelvic fin buds appeared in 22 hours and well developed in 38 hours old larvae. Mouth cleft and brain lobes were visible when the larvae were 34 and 38 hours old respectively. Myomeres partially appeared in 16 hours, which were clearly visible in 74 hours old larvae. Larvae started wandering and searching for food after 56 hours of hatching. The yolk sac was completely absorbed when larvae became 62 hours old.

Differential expression of the Brunol/CELF family genes during Xenopus laevis early development

The BRUNOL/CELF family of RNA-binding proteins plays important roles in post-transcriptional regulation and has been implicated in several developmental processes. In this study, we describe the cloning and expression patterns of five Brunol genes in Xenopus laevis. Among them, only Brunol2 is maternally expressed and the zygotic expression of the other four Brunol genes starts at different developmental stages. During Xenopus development, Brunol1, 4-5 are exclusively expressed in the nervous system including domains in the brain, spinal cord, optic and otic vesicles. Brunol2 and 3 are expressed in both the somatic mesoderm and the nervous system. Brunol2 is also extensively expressed in the lens. In transfected Hela cells, BRUNOL1, 2 and 3 proteins are localized in both the cytoplasm and the nucleus, while BRUNOL4 and 5 are only present in the cytoplasm, indicating their different functions.