Informe de la Segunda Reunión del Comité Examinador del Comité de Desarrollo y Cooperación del Caribe (CDCC), Puerto España, 20 febrero 1986 = Report of the Second Meeting of the Review Committee of the Caribbean Development and Co-operation Committee (CDCC), Port-of-Spain, 20 February 1986 = Rapport de la Deuxième Réunion du Comité d'Examen du CDCC, Port-of-Spain, 20 février 1986

Informe de la reunion que tuvo por objetivo principal examinar las actividades de los organismos especializados y otros organos en relacion con el programa de trabajo del CDCC en lo que concierne a la coordinacion del programa.

Purification and Characterization of an Ethanol-Tolerant β-Glucosidase from Sporidiobolus pararoseus and Its Potential for Hydrolysis of Wine Aroma Precursors

An extracellular ethanol-tolerant β-glucosidase from Sporidiobolus pararoseus was purified to homogeneity and characterized, and its potential use for the enhancement of wine aroma was investigated. The crude enzymatic extract was purified in four steps (concentration, dialysis, ultrafiltration, and chromatography) with a yield of around 40 % for total activity. The purified enzyme (designated Sp-βgl-P) showed a specific activity of approximately 20.0 U/mg, an estimated molecular mass of 63 kDa after sodium dodecyl sulfate polyacrylamide gel electrophoresis, and isoelectric point of 5.0 by isoelectric focusing. Sp-βgl-P has optimal activity at pH 4.0 and at 55 °C. It was stable in a broad pH range at low temperatures and it was tolerant to ethanol and glucose, indicating suitable properties for winemaking. The hydrolysis of glycosidic terpenes was analyzed by adding Sp-βgl-P directly to the wines. The released terpene compounds were evaluated by gas chromatography/mass spectrometry. The enzymatic treatment significantly increased the amount of free terpenes, suggesting that this enzyme could potentially be applicable in wine aroma improvement. © 2013 Springer Science+Business Media New York.

Training Seminar on Community Participation Methodology (I), Port-of-Spain, 29 March 1984

Includes bibliography

Urban management in medium-sized cities: final report of the case study Port-of-Spain, Trinidad and Tobago

Includes bibliography

A Municipal Information System for Port of Spain, Trinidad and Tobago

Includes bibliography