The importance of Leptospira interrogans serovars Icterohaemorrhagiae and Canicola in coastal zone and in southern fields of Rio Grande do Sul, Brazil

This study aimed to describe the occurrence of Leptospira interrogans serovars Icterohaemorrhagiae and Canicola, in coastal zone and in southern grasslands of Rio Grande do Sul, Brazil. In each one of the four analyzed farms blood samples were collected from free-living wild animals, domestic animals and humans to perform serological testing for leptospirosis. The presence of antibodies was verified by microscopic agglutination test (MAT). The criterion adopted to consider a serum as agglutination reactant was at least 50% of leptospira for a microscopic field of 100x. From 17 blood samples collected at Chuí, five (29.41%) were positive, three (60.00%) for serovar Icterohaemorrhagiae and two (40.00%) for Canicola. From 21 samples collected in the County of Santana da Boa Vista, six (28.57%) were positive, four (66.67%) for serovar Canicola and two (33.33%) for serovar Icterohaemorrhagiae. From 32 samples collected at Alegrete, 10 (31.25%) were positive, seven (70.00%) for serovar Icterohaemorrhagiae and three (30.00%) foro serovar Canicola. From 17 blood samples collected in Cruz Alta, three (17.64%) were positive, two (66.67%) for serovar Icterohaemorrhagiae and one (33.33%) for Canicola. It is necessary to improve sanitary practices on farms in the state of Rio Grande do Sul, in order to achieve success in leptospirosis control programs.

Bordering with Culture(s): Europeanization and Cultural Agency at the External Border of the European Union.

Rajallistamisen kulttuuri(t): Eurooppalaistuminen ja kulttuurinen toimijuus Euroopan unionin ulkorajalla Euroopan integraation ja Euroopan unionin laajentumisen myötä EU:n sisärajat ovat avautuneet kun taas sen ulkorajoilla lisääntyvää rajan ylitysten valvontaa on pyritty kompensoimaan yhteistyön merkitystä ja verkostoitumista painottamalla. Tämä tutkimus pyrkii ymmärtämään EU:n ulkorajan muutosten merkitystä paikalliselle hyvinvoinnille sekä laajemmin ylirajaisuuden merkitystä identiteettien rakentamiselle raja-alueilla. EU:n ulkorajalla Puolassa ja Suomessa toteutettavat rajat ylittävät, kulttuuriin ja kulttuuriperintöön liittyvät, projektit kertovat eurooppalaistumisesta ja sen vaikutuksista kulttuurisen horisontin muutokselle. Voidaan nähdä miten Eurooppa kulttuurisena konstruktiona tulee paikallisesti merkittäväksi tavoilla jotka kertovat myös paikallisten toimijoiden mahdollisuuksista osallistua EU-rajan muutoksia ja paikallisuutta määritteleviin prosesseihin. Tällöin erityisesti raja-alueiden materiaalisen perinnön, ja sen mahdollistamien rajaan liittyvien neuvottelujen, voidaan nähdä kertovat Eurooppalaistumisesta myös ns. alhaaltapäin muotoutuvana prosessina. Artikkeliväitöskirjan taustalla on Puolan ja Suomen toisen maailmansodan seurauksena luovuttamien raja-alueiden (Kresy ja Karjala) asema nykyisellä Euroopan unionin ulkorajalla. Tutkimusidea perustuu tutkijan omiin kokemuksiin projekteista Puolan ja Ukrainan raja-alueella vuonna 2003, ennen Puolan liittymistä EU:hun vuonna 2004. Tutkimusaineisto on peräisin vuosien 2005-2009 aikana tehdyistä ns. monipaikkaisista (multi-sited) kenttätöistä EU:n ulkorajalla, pääosin Puolassa ja Suomessa, joissa kohteena olivat kulttuuria ja kulttuuriperintöä hyödyntävät, pääosin EU-rahoitetut, rajat ylittävät projektit. Materiaalit koostuvat 34 projektitoimijan haastatteluista, projektien materiaaleista, kenttätöiden havainnoista, paikallisten sanomalehtien artikkeleista sekä eri tasoilla (EU, kansallinen, alueellinen) tuotetuista ohjelmadokumenteista. Huomio kiinnittyy projektitoimijoiden tapoihin tehdä rajanylityksiä, sekä heidän tapaansa kokea ja hyödyntää raja-alueiden kulttuuriperintöä sekä ymmärtää niiden nykyistä kulttuurista moninaisuutta. Tällöin havaitaan miten erilaiset eurooppalaiset ideat, representaatiot ja käytänteet tulevat osaksi erilaisia translokaaleja, rajat ylittäviä ja paikallis-eurooppalaisia, suhteita. Vertailun kohteeksi eivät tällöin asetu projektit, toimijat tai raja-alueet sinänsä, vaan näihin suhteisiin liittyvä kulttuurinen toimijuus. Keskeinen käsite tutkimuksessa on ’rajallistaminen’, eli sen havaitseminen, miten jokainen rajan ylitys tarkoittaa myös neuvottelua rajasta. Rajan ylitys voi siis tarkoittaa myös sen vahvistamista. Myös itse raja voi asettua toiminnan kohteeksi, jolloin nousee esiin se, miten rajat ylittäviä ”kulttuureja” käytetään ja mitkä ovat niiden rajaan liittyvät paikalliset merkitykset. Kysymys on siitä kuka, ja kenelle, rajan merkityksiä neuvottelee? Projektitoimijoiden voidaan nähdä neuvottelevan näitä erilaisia ”kulttuureja” jotka tuottavat rajaa neuvottelevia suhteita, kuten esimerkiksi yhteistyön verkostojen tapaa ohittaa rajan paikallinen merkitys. Tämä rajallistaminen voi kuitenkin tarkoittaa myös paikallisten kulttuuristen identifikaatioiden huomioimista. Tällöin kyse on myös sen luovuuden havaitsemisesta, jota yksilöillä on kun he neuvottelevat näitä erilaisia rajallistamisen kulttuureja. Erityisesti toisen maailmansodan seurauksena valtiorajoista tuli vahvasti kansallisia kulttuureja erottavia, mutta nyt kulttuurisista rajoista neuvotellaan ja rajojen yli tapahtuva vuorovaikutus, sekä paikallisen ja Eurooppalaisen tason väliset suhteet, ja niiden moniäänisyys, nousevat tutkimuksen keskiöön. Tutkimuksen yhteenvedon kannalta keskeinen on kysymys raja/alueen kestävyydestä. Tyypillisesti verkostoitumista painottavan rajat ylittävän yhteistyön suhde paikalliseen yhteisöön voi jäädä häilyväksi. Tavoite paikallisen kulttuuriperinnön suojeluun ei itsessään vielä kerro sen merkityksestä paikalliselle hyvinvoinnille. Arvioinnin kannalta on hyödyllistä nähdä miten myös materiaalisella perinnöllä on toimijuutta osana paikallisuutta muokkaavia suhteita. Paikallisten asukkaiden kokemus rajasta voi edelleen olla että se ei ole muuttunut Neuvostoliiton ajoista, toisaalta EU:nkin voidaan toivoa määrittelemään rajansa vielä tarkemmin, jotta sen kansallinen luonne muuttuisi. Tutkimus nostaa esiin miten eurooppalaiset yhteistyötä ja kulttuurista moninaisuutta korostavat ideat ja käytänteet vaikuttavat erityisesti puolalaisten toimijoiden mahdollisuuksiin määritellä EU-rajaan liittyviä prosesseja osana paikallisia kulttuuriperinnön määrittelyjä. Paikallisten rajaan liittyvien kulttuuristen identifikaatioiden liittäminen osaksi projekteja ei kuitenkaan ole helppoa. Toisaalta rajan merkitys on sisäistetty osana arkea, toisaalta taas rajaan liittyvät suuret kertomukset kansallisena ja EU-rajana voivat olla etäännyttäviä tekijöitä. EU-raja, projektit ja monikulttuurisen perinnön autenttisuus ovat kuitenkin raja-alueen toimijoille ja yhteisöille mahdollisuus osallistua rajallistamiseen. Toiminnan kestävyyden kannalta kyse on pitkälti siitä avaako rajallistaminen paikallisen perinnön merkityksiä osana paikallis-eurooppalaisia suhteita.

Occurrence of Campylobacter jejuni and C. coli on broiler carcasses after chilling in southern Brazil

Campylobacter jejuniand C. colihave been associated with gastrointestinal disorders in human beings, due mainly to the consumption of chicken meat. Despite control measures for reducing contamination by these bacteria, the detection of Campylobacter in carcasses after chilling remains high.A total of 105 carcasses were assessed by the horizontal detection method in five federally inspected slaughterhouses in southern Brazil in 2012 and in the first three months of 2013. Campylobacterwas isolated in 37.1% of the carcasses, of which 97.5% contained C. jejuni and 2.5% were infected by C. coli. The rate of positive carcasses across the slaughterhouses ranged from 0 to 71.4%. Determining the occurrence of Campylobacteramong flocks is crucial for estimating the microbial load at specific points along the slaughtering process and for minimizing the risk of contamination of end products by Campylobacter.

Oestrus ovis infection of grazing sheep during summer in southern Chile

Abstract: Oestrus ovis is a botfly whose larvae cause nasal myiasis, an environmental-dependent disease in small ruminants, generating acute and chronic injuries in the cranial cavities of sheep. Chile is a country of the southernmost worldwide distribution of this parasite, and there is few information about. Whence, the objective of this study was to approximate the epidemiological situation of O. ovis infection in Chilean sheep. From December 2009 to March 2010, a total of 87 samples were obtained by necropsy for skull inspection. The larvae were collected, and microscopically identified. The prevalence in the sampled sheep was 60.9%. From those that were infected, 85.7% (18/21) of sheep were 1 to 3 years old, constituting the stratum with the highest prevalence. The difference of infection in females and males was not significant. The high risk of infection seems to be dependent upon the environmental conditions of this southern region, especially during summer when the first larval stage (L1) could be found as the evidence. Therefore, the disease should be considered as a significant problem for this kind of livestock production.

Pythiosis in sheep from Paraná, southern Brazil

Abstract: This paper reports pythiosis in a sheep from southwestern Paraná, Brazil, confirmed by indirect ELISA (Enzime-Linked Immunosorbent Assay) and immunohistochemistry, as well as it describes the macro and microscopic injuries, in order to understand the pathogenicity. A 4-year-old ewe from a flock of 30 Santa Inês sheep, raised semi-extensively with access to a weir, showed cachexia, bilateral enlargement in nasal region, a serous and bloody secretion with a fetid odor from its nose and swollen submandibular and retropharyngeal lymph nodes. Blood collection was performed trough jugular vein puncture in order to make complete blood cell count (CBC) and to obtain serum for the subsequent serological examination. As the hematological counts were within the normal range for sheep, the animal was euthanized and submitted to necropsy. Indirect ELISA resulted positive for pythiosis. Necropsy revealed necrosis of the hard palate with a diameter of 3.5cm and extending up to the nasal cavity, forming a fistula. Submandibular and retropharyngeal lymph nodes were enlarged and edematous on section. Microscopic findings for submandibular and retropharyngeal lymph nodes consisted in moderate infiltration of eosinophils mainly in the subcapsular sinus, characterizing reactive eosinophilic lymphadenitis. The nasal cavity revealed rhinitis and oral cavity stomatitis with necro-eosinophilic and pronounced multifocal granulomatous infiltration and presence of hyphae. Hyphae found in palate and nasal cavity were positive for Pythium insidiosum by Grocott's method and immunohistochemistry, the last one considered to be confirmatory for the pathogen diagnostic. This report has an important epidemiological aspect, as it is the first case of pythiosis in sheep confirmed by serology in South Brazil and an alert of possible infection by the pathogen in floodplains.

Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil

Abstract: Canid herpesvirus 1 (CHV-1) is a widespread pathogen of dogs and produces infertility, abortions and severe systemic disease in young puppies. Clinical data indicate the circulation of CHV-1 among Brazilian dogs yet definitive diagnosis has rarely been accomplished. This article describes the clinicopathological findings of four independent cases/outbreaks of neonatal disease by CHV-1 in Bulldog puppies followed by virus identification and genetic characterization. Three events occurred in a kennel holding dogs of different breeds at reproductive age (March 2013, October 2013 and April 2014). Puppies from three French or English Bulldog litters, aging 9 to 30 days were affected, presenting dyspnea, agonic breathing, pale mucous, abdominal pain and tension, evolving to death within about 24 hours. At necropsy, the puppies presented necrohemorrhagic hepatitis, multifocal and moderate necrohemorrhagic nephritis and fibrinonecrotic interstitial pneumonia. Virus isolation was positive in clinical specimens from one litter and CHV-1 DNA was detected by PCR in tissues from all four cases. Virus-neutralizing assays with samples of the affected kennel revealed 9/12 adult animals with high antibody titers to CHV-1. Nucleotide sequencing of glycoprotein B, C and D genes revealed 99-100% of identity among the viruses and with CHV-1 sequences available in GenBank. Phylogenetic analyses of gC sequences showed a segregation of the samples, even among three isolates from the same kennel. These findings support CHV-1 infection as the cause of disease and death in these dog litters, reinforcing the need for correct etiologic diagnosis, prevention and immunization against CHV-1 in dogs from Southern Brazil.

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

Abstract The study was carried out to screen and analyze the genetic characteristics of antimicrobial resistance in Campylobacter spp. from poultry sources. A total of 141 strains of Campylobacter isolated from samples of broilers of slaughterhouses in southern Brazil was identified by phenotypic and genotypic methods. Campylobacter isolates were evaluated for its antimicrobial susceptibility and the presence of resistance genes. The strains were investigated for antimicrobial susceptibility against two agents (ampicillin and tetracycline) by disk diffusion method. PCR assay was used to confirm the specie and the presence of ampicillin (blaOXA-61), tetracycline tet(O), and the energy-dependent multi-drug efflux pump (cmeB) genes. Campylobacter jejuni was the most ubiquitous; its presence was determined in 140 samples out of 141 (99.3%), whereas Campylobacter coli was found only in one of the contaminated samples (0.70%). The results obtained showed 65% and 35.5% of Campylobacter isolates resistant to β-lactams and tetracyclines, respectively. The cmeB gene responsible for multidrug resistance was detected in 26 isolates out 141 strains (18.5%). Moreover, 36 out of 141 Campylobacter strains (25.6%) were found to be resistant to at least two different antimicrobia resistance markers (β-lactams and tetracyclines).

A field survey of crop management practices and distribution of ALS resistant Euphorbia heterophylla in two states in southern Brazil

Herbicide resistance was reported in Brazil almost ten years ago. One of the main weeds with herbicide resistance is wild poinsettia (Euphorbia heterophylla). This work evaluates the distribution of ALS-resistant E. heterophylla in two states in southern Brazil and determines the major contributing management causes for weed resistance selection in the area. E. heterophylla seeds from 148 sites located in Paraná and Rio Grande do Sul were sampled during 2001 and 2002. Farmers provided specific site data for weed control, tillage system, crop rotation and harvesting operations during previous years. ALS resistant E. heterophylla biotypes were found widely distributed in the survey area. Data analysis suggests seed dissemination is unlikely to explain the widespread distribution of resistance. The most probable factor for the selection of the resistant E. heterophylla is the persistent high use of ALS-inhibiting herbicides over time. Indirect evidence is presented demonstrating the need to educate legislators and farmers about the importance of herbicide mixtures as a strategy to prevent herbicide resistance.

Initial growth and development of southern sandbur based on thermal units

Availability of basic information on weed biology is an essential tool for designing integrated management programs for agricultural systems. Thus, this study was carried out in order to calculate the base temperature (Tb) of southern sandbur (Cenchrus echinatus), as well as fit the initial growth and development of the species to accumulated thermal units (growing degree days - GDD). For that purpose, experimental populations were sown six times in summer/autumn conditions (decreasing photoperiod) and six times in winter/spring condition (increasing photoperiod). Southern sandbur phenological evaluations were carried out, on alternate days, and total dry matter was measured when plants reached the flowering stage. All the growth and development fits were performed based on thermal units by assessing five base temperatures, as well as the absence of it. Southern sandbur development was best fit with Tb = 12 ºC, with equation y = 0,0993x, where y is the scale of phenological stage and x is the GDD. On average, flowering was reached at 518 GDD. Southern sandbur phenology may be predicted by using mathematical models based on accumulated thermal units, adopting Tb = 12 ºC. However, other environmental variables may also interfere with species development, particularly photoperiod.

Audio and Video Repositories at Scale: Indiana University’s Media Digitization and Preservation Initiative

Presentation at Open Repositories 2014, Helsinki, Finland, June 9-13, 2014

Some species of Perenniporia Murrill (Poriales, Basidiomycotina) from Southern Brazil

Perenniporia contraria, P. martius, P. medulla-panis, P. ohiensis, P. piperis, P. stipitata and P. tephropora are described in this paper. A key to these species is presented. Five species studied in culture are included, through the respective species code. P. tephropora is a new record from Southern Brazil. A check-list of names Perenniporia species known throughout the world is given.