G-1 Appeal Activity in the Public Assistance Programs, April 2003

Monthly report from the Iowa Department of Human Services

Biotic Factors Affecting Expression of the 2,4-Diacetylphloroglucinol Biosynthesis Gene phlA in Pseudomonas fluorescens Biocontrol Strain CHA0 in the Rhizosphere.

ABSTRACT Production of the polyketide antimicrobial metabolite 2,4-diacetyl-phloroglucinol (DAPG) is a key factor in the biocontrol activity of Pseudomonas fluorescens CHA0. Strain CHA0 carrying a translational phlA'-'lacZ fusion was used to monitor expression of the phl biosynthetic genes in vitro and in the rhizosphere. Expression of the reporter gene accurately reflected actual production of DAPG in vitro and in planta as determined by direct extraction of the antimicrobial compound. In a gnotobiotic system containing a clay and sand-based artificial soil, reporter gene expression was significantly greater in the rhizospheres of two monocots (maize and wheat) compared with gene expression in the rhizospheres of two dicots (bean and cucumber). We observed this host genotype effect on bacterial gene expression also at the level of cultivars. Significant differences were found among six additional maize cultivars tested under gnotobiotic conditions. There was no difference between transgenic maize expressing the Bacillus thuringiensis insecticidal gene cry1Ab and the near-isogenic parent line. Plant age had a significant impact on gene expression. Using maize as a model, expression of the phlA'-'lacZ reporter gene peaked at 24 h after planting of pregerminated seedlings, and dropped to a fourth of that value within 48 h, remaining at that level throughout 22 days of plant growth. Root infection by Pythium ultimum stimulated bacterial gene expression on both cucumber and maize, and this was independent of differences in rhizosphere colonization on these host plants. To our knowledge, this is the first comprehensive evaluation of how biotic factors that commonly confront bacterial inoculants in agricultural systems (host genotype, host age, and pathogen infection) modulate the expression of key biocontrol genes for disease suppression.

G-1 Appeal Activity in the Public Assistance Programs, May 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, June 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, July 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, August 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, September 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, October 2003

Monthly report from the Iowa Department of Human Services

G-1 Appeal Activity in the Public Assistance Programs, November 2003

Monthly report from the Iowa Department of Human Services

Piispa E.G. Gulin 21/10 1964

Kirje 21.10.1964

Use of alcohol in the elderly: transcultural validation of the Michigan Alcoholism Screening Test – Geriatric Version (MAST-G)

Objective: To evaluate the internal consistency of the version of the Michigan Alcoholism Screening Test – Geriatric Version (MAST-G) instrument, translated and adapted for Brazil. Method: This was a descriptive, cross-sectional study. Data were collected through a demographic questionnaire, the ICD-10 and the MAST-G, following the steps of translation and cultural adaptation. One hundred eleven elderly in the city of São Carlos, SP, Brazil were interviewed. Results: The mean age of those interviewed was 70 years, with 45% men and 55% women, with the mean education of three years; 92% resided with family; 48% of the subjects consumed alcoholic beverages. The MAST-G presented a good level of reliability, with Cronbach’s α = 0.7873, and good levels of sensitivity and specificity with a cutoff score of five positive responses. Conclusion: The Brazilian version of the MAST-G presented internal consistency values similar to the original English version,showing it to be adequate for use in the national context.





G-1 Appeal Activity in the Public Assistance Programs, August 2006

G-1 Appeal Activity in the Public Assistance Programs

G-1 Appeal Activity in the Public Assistance Programs, September 2006

G-1 Appeal Activity in the Public Assistance Programs

G-1 Appeal Activity in the Public Assistance Programs - October 2006

G-1 Appeal Activity in the Public Assistance Programs - October 2006