928 resultados para Plantas medicinais - Quantificação - Brasil


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In this work, it was developed and validated methodologies that were based on the use of Infrared Spectroscopy Mid (MIR) combined with multivariate calibration Square Partial Least (PLS) to quantify adulterants such as soybean oil and residual soybean oil in methyl and ethyl palm biodiesels in the concentration range from 0.25 to 30.00 (%), as well as to determine methyl and ethyl palm biodiesel content in their binary mixtures with diesel in the concentration range from 0.25 to 30.00 (%). The prediction results showed that PLS models constructed are satisfactory. Errors Mean Square Forecast (RMSEP) of adulteration and content determination showed values of 0.2260 (%), with mean error (EM) with values below 1.93 (%). The models also showed a strong correlation between actual and predicted values, staying above 0.99974. No systematic errors were observed, in accordance to ASTM E1655- 05. Thus the built PLS models, may be a promising alternative in the quality control of this fuel for possible adulterations or to content determination.

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Nicaragua importa semilla de pasto con el objetivo de resolver la baja productividad y calidad del pasto. Las especies Brachiaria brizantha y Panicum maximun, son las semilla de pasto de mayor importación en nuestro país, siendo Brasil el país de donde más importamos. Basados en que existen algunas plagas en Brasil que no existen en Nicaragua, se procedió a realizar un análisis de riesgo de plaga, el cual es una herramienta que permite evaluar la entrada, establecimiento, dispersión y manejo del riesgo de la plaga. Las etapas del análisis de riesgo de plaga; se realizó con la recopilación y análisis de información clave de fuente primaria y secundaria como son la lista de plagas asociadas al cultivo de pasto de Brasil, lista oficial de plagas asociadas al cultivo pasto en Nicaragua, diagnóstico fitosanitario en plantaciones de pasto B. brizantha en Nicaragua y los resultados de laboratorio de las semilla de pasto de origen Brasil que ingresaron por el país en el 2014. Este estudio se realizó en un período de 12 meses de Enero a Diciembre del 2014, en la Región Autónoma del Caribe Sur (RACS), en el municipio de El Rama y dos departamentos Chontales y Rio San Juan en los municipios de La Gateada y San Miguelito respectivamente, ya que son representativos, son las zonas de mayor producción de pasto, y en los doce puesto de cuarentena agropecuaria del territorio nacional, El Guasaule, Puerto Corinto, Aduana Central Aérea, Las Manos, El Espino, Peñas Blancas, Teotecacinte, Aeropuerto Internacional Augusto César Sandino, El Rama, El Bluff, San Carlos y San Juan de Nicaragua, que es donde ingresan las importaciones de semilla de pasto de origen Brasil. El muestreo fue al azar, tanto para el diagnóstico fitosanitario como para las importaciones de semilla de pasto de origen Brasil, tomando muestras para ser analizadas en los laboratorios de herbología, entomología, micología, bacteriología y nematología. El diagnóstico fitosanitario, permitió corroborar la presencia de plagas mencionadas en la lista oficial de plagas asociadas al cultivo pasto en Nicaragua y descartar presencias de plagas cuarentenarias en los pasto de nuestro país. En las semilla de pasto importadas de Brasil, se determino la presencia de plagas de interés cuarentenario como son la maleza Commelina benghalensis y nemátodo de punta blanca Aphelenchoides besseyi. En el análisis de riesgo de plaga, se identificaron siete plagas de interés cuarentenario para la especie B. brizantha y noventa y uno plagas para la especie P. maximun, al evaluar cada plaga por la posibilidad de seguir la vía de entrada, se determinaron dos plagas de importancia cuarentenarias para el área del análisis de riesgo de plagas, como son la maleza Commelina benghalensis L. y nemátodo de punta blanca Aphelenchoides besseyi Ch., ya que son plagas consideradas de alto riesgo fitosanitario debido a la posibilidades de sobrevivir, multiplicarse y diseminarse en el ambiente de Nicaragua una vez introducida en nuevas aéreas establecidas, de acuerdo a la evaluación del riesgo de establecimiento y dispersión; es por eso que en ambas plagas se determinaron las opciones del manejo del riesgo para disminuir a niveles adecuados los riesgo de dichas plagas para nuestro país.

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The strong selection pressure exerted by intensive use of glyphosate in cultivated areas has selected populations of the Rubiaceae weed species Borreria latifolia (Aubl.) K.Shum. (broadleaf buttonweed), Galianthe chodatiana (Standl.) E.L. Cabral (galiante) and Richardia brasiliensis Gomes (Brazilian pusley) with differential sensitivity to this herbicide in the South region of Brazil. The control of these weeds with herbicides is troublesome and signals the need to incorporate management practices of ruderal flora and crops, more sustainable and that results in more efficient control for long-term. Therefore, it is very important to expand the information about their biology and management. This study aimed: (a) select efficient methods to overcome dormancy of B. latifolia and G. chodatiana and determine how they influence the kinetics of seeds germination; (b) analyze the effects of temperature, irradiance, pH, aluminum and salinity on seed germination and initial growth of the B. latifolia, G. chodatiana e R. brasiliensis seedlings; (c) evaluate tolerance to glyphosate levels in biotypes of B. latifolia, G. chodatiana e R. brasiliensis through dose-response curves and compare two methods to evaluate herbicidal control; (d) and evaluated the effectiveness of alternative herbicides in pre-emergence and in early and late post-emergence of the three species. The treatment with KNO3 2%/3h + gibberellic acid 400 ppm resulted in higher percentage of G. chodatiana seed germination. This treatment and also the dry heat (60°C/30 min) + KNO3 2%/3h were more effective in overcoming dormancy of B. latifolia. G. chodatiana and R. brasiliensis tolerate lower temperatures during the germination process, while B. latifolia tolerate higher temperatures. B. latifolia and R. brasiliensis are positive photoblastic while G. chodatiana is indifferent to the photoperiod. B. latifolia shows higher germination and early development in pH 3, while G. chodatiana and R. brasiliensis prefer pH range between 5 and 7. B. latifolia and G. chodatiana were more tolerant to the aluminum during the germination process than R. brasiliensis. Low salt levels were sufficient to reduce the seed germination of the three species. Some biotypes of B. latifolia and R. brasiliensis showed medium-high glyphosate tolerance, not being controlled by higher doses than recommended. The G. chodatiana specie was not controlled with the highest dose used, showing a high glyphosate tolerance. The sulfentrazone, s-metolachlor and saflufenacil herbicides sprayed in pre-emergence showed high efficacy both on B. latifolia and R. brasiliensis, while chlorimuron-ethyl and diclosulan were effective only on R. brasiliensis. In early post-emergence the fomesafen, lactofem and flumioxazin herbicides efficiently controlled plants of all species, while bentazon showed high efficacy only on B. latifolia. Noteworthy the susceptibility of the G. chodatiana specie for applications in early post-emergence, because the control effectiveness and the number of effective herbicides are reduced with increasing the plant age. Many treatments with tank mix or sequencial applications with glyphosate, were effective in controlling B. latifolia and R. brasiliensis plants in advanced stage of development.

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Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae ) is an insect from Australia which is causing severe damage to eucalyptus crops around the world. When feeding from the leaves sap, it causes bronzening, and in extreme cases, may lead to the tree death. Control methods have been studied and the most promising so far is the egg parasitoid Cleruchoides noackae (Hymenoptera: Mymaridae). Alternative products from plants with insecticidal properties could also be a viable option, and they might even be used concomitantly with C. noackae, aiming for a most effective control, but still safe for the environment. Thus, the objective of this work was to verify the action of 5% aqueous plant extracts of Matricaria chamomilla, Echinodorus grandiflorus, Punica granatum, Maytenus ilicifolia a n d Origanum majorana on T. peregrinus. In addition, we aimed to study the extracts potential toxicity to C. noackae and Gallus domesticus L., since the plant compounds might have negative effect upon the non-target organisms. At first, HPLC (High Performance Liquid Chromatography) was used to verify which phenolic compounds would be found in the plant extracts. These were tested on bronze bug adults, in confinement test (to verify the insecticidal action of the extracts) and free-choice test (to verify the repellency). The extracts that showed better results were selected for further tests with non-target organisms. Regarding C. noackae, pre-parasitism and post-parasitism, confinement and free-choice tests were performed to verify if the extracts would affect the host-choosing by the female or the development of the immature stages of the parasitoid. To verify if the extracts would be toxic to G. domesticus, the plant extracts were added to young birds feed for five days. Parameters such as weight gain, food intake, quantification of serum enzymes and histopathological analysis were carried out. HPLC analysis detected gallic, ferulic, vanillic, caffeic and cumaric acid in the extracts samples. All plant extracts tested reduced T. peregrinus survival, but E. grandiflorus, Matricaria chamomilla Maytenus ilicifolia had also a repellent effect, and were tested on the non-target organisms. None of these extracts affected neither the host choice by C. noackae nor adult emergency, when compared to the control group. In addition, the extracts did not cause alterations in any of the studied parameters. Thus, we verified that E. grandiflorus, Matricaria chamomilla and Maytenus ilicifolia have potential to be used to control T. peregrinus and are safe to C. noackae and G. domesticus.

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A colonização por invertebrados aquáticos no detrito de Salvinia herzogii foi estudada em dois lagos rasos subtropicais de diferentes estados tróficos (eutrófico e oligotrófico) localizados no campus Carreiros da Universidade Federal do Rio Grande, extremo sul do Brasil. Aproximadamente 6g (peso úmido) de S. herzogii foram incubados em experimentos com litter bags (30 X 20 cm com 10 mm de malha) e a decomposição do detrito acompanhada durante 110 dias (entre setembro de 2008 e janeiro de 2009), período no qual o detrito decompôs-se cerca de 95%. Quatro réplicas foram retiradas após os intervalos de 1, 30, 60, 90 e 110 dias de incubação e o detrito foi limpo em água corrente sob peneira de 250µm de malha para que fossem retidos os organismos e depois fixados em álcool 80%. As plantas depois de limpas foram secas à 60ºC por 72 horas para obtenção do peso seco, depois trituradas, para as análises de nitrogênio e fósforo total e para obtenção do peso seco livre de cinzas, equivalente a porcentagem de massa remanescente (%R). Os invertebrados foram identificados até o menor nível taxonômico possível e depositados na coleção de Invertebrados Límnicos – ICB - FURG. Para cada amostra foram calculadas a riqueza e a densidade de táxons, os índices de diversidade de Shannon - Wiener (H’), homogeneidade de Pielou (J). Para determinar qual grupo de invertebrados foi mais importante para a estruturação da comunidade em cada lago, foi realizada a análise de espécies indicadoras (indicator especies analysis). Um total de 32.399 organismos distribuídos em 38 táxons foram registrados para os lagos estudados. Dezoito táxons foram comuns entre os lagos em cada experimento. Um total de 1.449.612 ind. 100g PS no ambiente eutrófico e 372.380 ind. 100g PS para o ambiente oligotrófico foi registrado (p=0,4771). O táxon mais abundante encontrado para o lago eutrófico foi Goeldichironomus (Chironomidae; 81% de todos os organismos coletados), enquanto queno lago oligotrófico, o táxon mais abundante foi Caenis (Caenidae; 29,1% de todos os organismos coletados). A colonização do detrito foi rápida, nas primeiras 24 horas foram registrados 10 táxons nos lagos eutrófico e oligotrófico, sendo os predadores o grupo dominante (52 e 70%, respectivamente) neste período. Durante o período do experimento,no lago eutrófico, os coletores-catadores foram os mais abundantes (91,2 % do número total de organismos), seguido de coletores-filtradores (4,7 %) e predadores (3,5 %). No lago oligotrófico, os coletores-catadores foram os mais abundantes (34,1 % do número total de organismos), seguido de predadores (26,8 %), raspadores (24,9 %) e fragmentadores (10,5 %). O Índice de diversidade acompanhou os aumentos de densidade durante os experimentos, sendo que o valor máximo observado foi no lago oligotrófico(H’=2,31) no 60º dia de incubação, enquanto que o menor valor foi registrado para o lago eutrófico (H’=0,19) no 110º dia de incubação. A taxa de decomposição de S. herzogii foi diferente no lago eutrófico (k = 0,019; R2 = 0,88) e oligotrófico (k = 0,021; R2 = 0,81),porém essa diferença não foi significativa (ANCOVA; F = 6,38 e p = 0,6755).

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The no-tillage system is the predominant model in the agricultural scenario of southern Brazil. Thus, the use of cover crops is significant due to the addition of biomass to protect the soil surface, and contribute to the cycling and/or fixing of nutrients, and in particular nitrogen (N) with liberation for the subsequent culture. Among the cool season species, it was found predominant use of oat to obtain straw to system. Though large quantities input of residue is not the preferred species to precede the corn, cereal with relevant importance in the Paraná Southwest region. It was aimed to evaluate the productivity capacity of corn in no-tillage, in the absence or presence of nitrogen fertilization, on waste of winter cover crops on soil and climatic conditions of the Paraná Southwest region. The installation of no-tillage was held in 2010 in the experimental area belonging to UTFPR, Campus Dois Vizinhos, on a Red Latosol. For the present study, we used data relating to three agricultural years (2012/2013, 2013/2014 and 2014/2015). The experimental design was randomized block design with split plots with three replications. The main plots consisted of systems composed by cover crops (black oat, ryegrass, rye, turnip, vetch, white lupine, aot+vetch consortium and oat+vetch+turnip), preceding corn. In the subplots were used two doses of nitrogen fertilization (0 and 180 kg ha N) coverage in maize.The biggest coverage rates occurred in the consortium with 95% at 62 days after sowing. The residual effect of 180 kg ha cool season plants following year. The residual effect of 180 kg ha systems, reduced in 21% the C/N ratio of poaceae. The common vetch accumulated 32 kg N per ton of MS added. The oat and rye keeps more than 50% waste to the land cover, after 120 days, while the ryegrass and vetch provide low soil protection. Consortium oat+vetch+turnip, vetch and white lupine, released the largest amounts of N, between 52 and 59 kg ha brassica and consortia positively influencing the diameter and length of cobs, number of kernels per row and, total number of grains per ear of corn, in the absence of mineral N. The weight of a thousand grains was increased by 12.4% by the addition of 180 kg ha increase in productivity of grain by the addition of 180 kg ha N, was 2.1 Mg ha 5.6 Mg ha 6.4 Mg ha components when cultivated on vetch. Systems containing fabaceae, brassica and consortium oat+vetch+turnip, predating the corn, in the absence of mineral N, provided similar grain yelds inrelation to the systems with the addition of 180 kg ha Keywords: Cover crops. No-tillage. Grain yield. Zea mays - 1 -1 N, increased 4.8% coverage rate in the of N in corn/cover crops -1 -1 . Fabaceae, -1 N mineral. The average N, in relation to dose 0 kg ha corn kernels on fabaceae, brassica and consortium oat+vetch+turnip, and poaceae the grains in succession. The consortium added amount between 4.0 the DM in the years of study. There was no effect of mineral N rate for corn yield components when cultivated on vetch. Systems containing fabaceae, brassica and consortium oat+vetch+turnip, predating the corn, in the absence of mineral N, provided similar grain yelds inrelation to the systems with the addition of 180 kg ha-1 N.

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The fungus Rhizoctonia solani is a soil borne pathogen that causes damage to various crops. The chemical control, when managed incorrectly, can be harmful to the environment, which makes the study of alternative control important. This study aimed to evaluate the ability of different doses of Liquid swine manure (LSM), with and without the retention of gases, at different soil pH levels, to control R. solani in beet. An inoculum of the fungus R. solani was on rice grains, which had been previously sterilised. The experiments were set up in a greenhouse in a completely randomised block design, arranged in a three-factor 2 x 2 x 5 scheme, comprising of soil pH levels (4.8 and 7.2) x with and without gas retention x LSM dose (0, 5, 10, 15 and 20%), with four replications per treatment. To setup the experiments, 4 kg of soil of each pH level were packed separately into plastic bags. Subsequently, the soil of each bag was infested with 15 g of fungus inoculum/kg of soil, and moistened as necessary. After seven days of infestation of the soil with the pathogen the different doses of LSM were incorporated separately into the bags, the bags designated as the gas retention treatment were closed, while those designated as the gas release treatment were left open. After seven days, part of the soil from each bag was packed separately into 16 cells of 128 cell Styrofoam trays, which were then seeded with two beet seeds per cell. The other part of the soil was placed in 2 litre pots, to conduct the quantification of microbial activity, through the method of CO2 release, 21 days after the experiment was setup. Seedling emergence and damping-off evaluations were performed daily for 21 days consecutively. The data was submitted to analysis of variance, and when significant were submitted to regression analysis or Tukey at 5% probability of error. The experiments were repeated twice. According to the results obtained, there was a suppressive effect of LSM on R. solani. For the variable emergence, the 10% dose of LSM resulted in the largest number of emerging plants in the two soil pH levels studied, whether or not gas was retained. Seedling dampingoff decreased with increasing volumes of LSM incorporated into the soil. The soil with the pH level of 7.2 presented less seedling damping-off than the soil with a pH level of 4.8. The retention of gases provided greater control of R. solani in the higher LSM doses and in soil with a pH level of 7.2. Also noted in this study that there was a significant increase in microbial activity with increasing doses of LSM when applied to soil with pH levels of 4.8 and 7.2. Based on these results, it was concluded that the 10% dose of LSM provided the best control of R. solani without harming seedling emergence.

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To achieve high yields the corn crop is dependent on nitrogen. Systems of cover crops preceding corn and form of land cultivation are essential for the best use of nitrogen by corn. This study aimed to evaluate the use or not of nitrogen fertilization in corn in succession to cover crops, planted in three cropping systems. The experimental design was randomized blocks with sub-divided portion where the main plots consisted of three cultivation systems (tillage, conventional tillage and minimum tillage), the subplots by four plant cover in monocrop (oat, hairy vetch, field peas and turnip) and sub-subplots by nitrogen fertilization (0 and 160 kg ha-1 N). Evaluations were performed, the cover crops, soil cover rate, dry matter, content and accumulation of nutrients. In corn we evaluated yield components, yield, chlorophyll and nutrient levels in leaves. Among the species coverage studied the oat showed hardiness in the experiment, covering ground faster and showing more dry matter, however vetch hairy showed higher concentrations of N, P and K and higher accumulation of N ha-1. The soil tillage system influenced the K leaf content. The interaction cultivation x coverage showed significance for the total chlorophyll of corn. In the absence of N, N content and chlorophyll were higher where the corn was sown on the pea and hairy vetch. The corn production, despite a higher average in the presence of nitrogen fertilization, did not differ significantly when used the pea and hairy vetch as a cover crop.

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El artículo intenta discutir la formación de la identidad médica a partir del análisis del proceso de descalificación oficial de los curanderos y de los esfuerzos por diferenciar el conocimiento médico de la llamada charlatanería en Río de Janeiro de la primera mitad del siglo XIX. En este sentido, se trata de señalar la diferencia entre el período en que las actividades de los curanderos eran legitimadas por la Fisicatura-mor (órgano del gobierno responsable por la normatividad de las prácticas de cura) a partir de la concesión de licencias a los mismos y luego para el momento en que los médicos organizados en instituciones y articulados políticamente comienzan el proceso de descalificación de los curanderos populares y también en la apropiación de sus conocimientos. El artículo analiza las licencias y peticiones así como discursos de políticos del período para demostrar la búsqueda de la hegemonía social del conocimiento médico, principalmente por la asociación del conocimiento de las plantas dominadas por los curanderos populares y la legitimidad de la población hacia ellos.

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The screening for genes in metagenomic libraries from soil creates opportunities to explore the enormous genetic and metabolic diversity of microorganisms. Rivers are ecosystems with high biological diversity, but few were examined using the metagenomic approach. With this objective, a metagenomic library was constructed from DNA soil samples collected at three different points along the Jundiaí-river (Rio Grande do Norte-Brazil). The points sampled are from open area, rough terrain and with the direct incidence of sunlight. This library was analyzed functionally and based in sequence. For functional analysis Luria-Bertani solid medium (LB) with NaCl concentration varied from 0.17M to 0.85M was used for functional analysis. Positives clones resistant to hypersaline medium were obtained. The recombinant DNAs were extracted and transformed into Escherichia coli strain DH10B and survival curves were obtained for quantification of abiotic stress resistance. The sequences of clones were obtained and submitted to the BLASTX tool. Some clones were found to hypothetical proteins of microorganisms from both Archaea and Bacteria division. One of the clones showed a complete ORF with high similarity to glucose-6-phosphate isomerase which participates in the synthesis of glycerol pathway and serves as a compatible solute to balance the osmotic pressure inside and outside of cells. Subsequently, in order to identify genes encoding osmolytes or enzymes related halotolerance, environmental DNA samples from the river soil, from the water column of the estuary and ocean were collected and pyrosequenced. Sequences of osmolytes and enzymes of different microorganisms were obtained from the UniProt and used as RefSeqs for homology identification (TBLASTN) in metagenomic databases. The sequences were submitted to HMMER for the functional domains identification. Some enzymes were identified: alpha-trehalose-phosphate synthase, L-ectoina synthase (EctC), transaminase L-2 ,4-diaminobutyric acid (EctB), L-2 ,4-diaminobutyric acetyltransferase (EctA), L-threonine 3 dehydrogenase (sorbitol pathway), glycerol-3-phosphate dehydrogenase, inositol 3-phosphate dehydrogenase, chaperones, L-proline, glycine betaine binding ABC transporter, myo-inositol-1-phosphate synthase protein of proline simportadora / PutP sodium-and trehalose-6-phosphate phosphatase These proteins are commonly related to saline environments, however the identification of them in river environment is justified by the high salt concentration in the soil during prolonged dry seasons this river. Regarding the richness of the microbiota the river substrate has an abundance of halobacteria similar to the sea and more than the estuary. These data confirm the existence of a specialized response against salt stress by microorganisms in the environment of the Jundiaí river

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Química, Programa de Pós-Graduação em Tecnologia Química e Biológica, 2016.

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Dissertação de Mestrado, Biologia Marinha, Faculdade de Ciências e Tecnologias, Universidade do Algarve, 2014

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El incremento en la perturbación ambiental y la presión mundial para preservar la selva amazónica han fomentado el cultivo de especies nativas. Con el objetivo de evaluar el crecimiento de Schizolobium parahyba var. amazonicum (Huber ex Ducke) barneby cultivado en presencia de componentes agrícolas como Ananas comosus var. erectifolius, se desarrolló un estudio en la Estación Experimental de la empresa Tramontina Belem S.A., ubicada en la Ciudad de Aurora do Pará (PA), Brasil. El diseño experimental fue factorial de bloques completos al azar con parcelas subdivididas en el tiempo, para que los arreglos formaron los tratamientos de la parcela y las subparcelas fueron el tiempo de observación, con seis tratamientos y cuatro repeticiones: 1) S. parahyba var. amazonicum, Cordia goeldiana y Switenia macrophylla:GLC; 2) S. parahyba var. amazonicum, C. goeldiana, S. macrophylla y A. comosus var. erectifolius: GLCc; 3) S. parahyba var. amazonicum y C. goeldiana: GL; 4) S. parahyba var. amazonicum, C. goeldiana y A.comosus var. erectifolius; GLCc; 5) S. parahyba var. amazonicum: G; y 6) S.parahyba var. amazonicum y A. comosus var. Erectifolius: Gc. La distancia entre las especies forestales fue 4 x 3 m y para las plantas de A. comosus 0.80 m x 0.50 m. El tamaño de la parcela fue 18 x 24 m con cuatro repeticiones por tratamiento, el total de parcelas fue 24 y de área experimental 10 368 m2. Las variables analizadas para inferir el crecimiento del componente forestal fueron altura (H) y diámetro a la altura del pecho (DAP), cada 6 meses durante 3 años. En los seis sistemas de cultivo la tasa semestral de crecimiento de la altura y DAP de S. parahyba var. amazonicum fue mayor en los dos primeros años, y siguió con una tendencia de crecimiento lento y continuo durante los 36 meses de estudio. El efecto positivo de la presencia de A. comosus var. erectifolius se observó en el crecimiento de las plantas de S. parahyba var.amazonicum.