牛胚胎在BRL/mTCM199和HECM-6/mTCM199培养体系中发育潜能的探讨

将受精卵置于下列两种培养体系中进行体外培养:(1)Buffalo rat liver细胞与改进的TCM199培养液共同培养系统(BRL/mTCM199);(2)半确定培养液两步培养系统[无血清的金黄地鼠培养液与改进的TCM199培养液(HECM-6/mTCM199]。结果表明:后者的囊胚率(50%)明显高于前者的囊胚率(41%,P<0.01)。表明牛胚胎早期分裂可在无蛋白培养条件下进行,并且在此培养条件下有良好的后续发育能力。这可能与两步培养体系中葡萄糖和氨基酸浓度不同有关。

The effect of artisanal preservation methods on nutritional security of “Mukene” Rastrineobola argentea caught from Lakes Victoria and Kyoga in Uganda

The artisanal fish preservation methods in Uganda are characterized by extreme operating conditions. Consequently, vital nutritional components diminish in value and quantity which renders fish consumer nutritionally insecure. To establish the magnitude of nutritional loss, duplicate samples of Mukene Rastrineobola argentea were collected from Kiyindi landing site on L. Victoria and Moone landing site on L. Kyoga. Each set of duplicate samples was divided into five portions and kept on ice. For each preservation method a portion was processed into respective products at Food Bioscience and Agri-Business Laboratories aside from the control (fresh) sample. Both preserved and control samples were analysed for nutrient loss at Department of Chemistry, Makerere University using AOAC methods. The composition of fatty acids was determined by methanolysis gas chromatography and Mass spectrophotometry of the resultant methyl esters. The results indicate that nutrients of all preserved samples did not vary significantly from the control except for some fatty acids. The Eicosapentaenoic acid (EPA) in fresh samples declined from 6.72% to 1.08% in deep-fried samples constituting 83.93% nutrient loss. The sum ratio w3:w6 as well as EPA: DHA (Docosahexaenoic) ratio in fried samples also varied significantly (p<0.5) lower than 0.668 and 0.20 for the average of either preservation methods and experts recommended ratio respectively. Further research has been recommended to ascertain the causative factor, since Mukene frying is being promoted in the Great lakes region as alternative method to sun-drying. In conclusion, regular consumers of fried Mukene do not benefit much from the nutritional and health attributes of Omega 3 and 6.

Hardfacing using supersonic laser depostion of stellite-6

The capability of manufacturing coatings is of central importance in engineering design. Many components require nowadays the application of additional layers, to enhance mechanical properties and protect against hostile environments. Supersonic Laser Deposition (SLD) is a novel coating method, based upon Cold Spray (CS) principles. In this technique the deposition velocities can be significantly lower than those required for effective bonding in CS applications. The addition of laser heat energy permits a change in the thermodynamic experience of impacting particles, thereby offering a greater opportunity for metallurgical bonding at lower velocities compared to the CS process technology. The work reported in this paper demonstrates the ability of the SLD process to deliver hard facing materials to engineering surfaces. Stellite-6 has been deposited on low carbon steel tubes over a range of process parameters, determining the appropriate target power and traverse speeds for coating deposition. Coating properties and parameters were examined to determine the main properties, micro-structure and processing cost. Their morphology was studied through optical microscopy, SEM and X-Ray Diffraction. The results have shown that SLD is capable of depositing Stellite-6, with enhanced properties compared to laser clad counterparts.

Seminario sobre ciencias básicas como prerequisito para la enseñanza de la oceanografía (1-2 octubre de 1962)- Seminario sobre biogeografía de los organismos marinos (3-6 octubre de 1962)

Por acuerdo del Seminario Latinoamericano sobre Estudios Oceanográficos realizado en la Universidad de Concepción, Chile, 20-25 noviembre de 1961, se recomendó la realización en Argentina de un Seminario sobre Biogeografía de Organismos Marinos, indicándose al Museo Argentino de Ciencias Naturales Bernardino Rivadavia como sede del mismo. Luego de varias conversaciones en Buenos Aires (Argentina), entre autoridades del Centro de Cooperación Científica para América Latina, del CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), del Servicio de Hidrografía Naval, del mencionado Museo y del Instituto de Biología Marina de Mar del Plata, se acordó realizar los seminarios en éste último instituto del 1 al 6 octubre de 1962. Del 1 al 2 de octubre, se realizó el Seminario sobre Ciencias Básicas como prerrequisito para la Enseñanza de la Oceanografía, pero los documentos no fueron incluídos en este Boletín. Del 3 al 6 de octubre, tuvo lugar el Seminario sobre Biogeografía de Organismos Marinos, con la participación de investigadores de: Brasil, Colombia, Costa Rica, Chile, Mexico, Perú, Uruguay, Venezuela y de Argentina, y se incluyeron los siguientes trabajos publicados: Joly, A. - Extensao da flora marinha no sul do Brasil- nota preliminar; Etcheverry Daza, H. - Distribución geográfica de las algas del Pacífico; Diaz-Piferrer, M. - Biogeografía de las algas marinas tropicales de la costa Atlántica de America, Resumen,; Kuhneman, O. - Importancia de la vegetación en biogeografía marina; Boschi, E.E. - Los peneidos de Brasil, Uruguay y Argentina; Bernasconi, I. - Distribución geográfica de los equinoideos y asteroideos de la extremidad austral de Sudamérica; Szidat, L. - La parasitología como ciencia auxiliar para la biogeografía de organismos marinos; López, R.B. - Problemas de la distribución geográfica de los peces marinos sudamericanos; Ximénez, I. - Estudio preliminar de la distribución geográfica actual de los pinípedos en América Latina; Balech, E. - La división zonal en biología marina y su nomenclatura; Stuardo, J. - Distribución de los moluscos marinos litorales en Latinoamérica; Boltovskoy, E. - Provincias zoogeográficas de América del Sur y su sector Antártico según los foraminíferos bentónicos; Rioja, E. - Caracteres de la biogeografía marina de México y de Centro América; Balech, E. - Caracteres biogeográficos de la región de Argentina y Uruguay; Vannucci, M. - Zoogeografía marinha do Brasil.

基于部分线粒体细胞色素氧化酶亚基Ⅰ(mtCOI)DNA 序列的6种蝙蝠(翼手目:蝙蝠科)的分子系统进化关系

对贵州5种蝙蝠科蝙蝠的部分线粒体细胞色素氧化酶亚基Ⅰ DNA序列进行了测定,并结合从Genbank获得的爪哇伏翼的相应序列,以Pteropus dasymallus,P.scapulatus,Rousettus aegyptiacus为外群,运用贝叶斯法(Bayesian),最大似然法(Maximum Likelihood,ML)分析了这6种蝙蝠科蝙蝠的分子系统进化关系.结果表明:在贝叶斯,ML树中,这6种蝙蝠科的蝙蝠可分为3个分支:亚洲长翼蝠是第1个独立出来的分支;白腹管鼻蝠是继亚洲长翼蝠之后第2个分离出来的分支;第3个分支又分为两支,一支由大鼠耳蝠和小鼠耳蝠组成,另一支由南蝠和爪哇伏翼组成,支持将这4种蝙蝠同归于蝙蝠亚科的结论,从一定程度上否定了鼠耳蝠属和管鼻蝠亚科之间的姐妹类群关系,也不支持将鼠耳属提升为亚科.

Tests for conceptual design of 6.6 kV class superconducting fault current limiter with YBCO thin film elements

A superconducting fault current limiter (SFCL) for 6.6 kV and 400 A installed in a cubicle for a distribution network substation was conceptually designed. The SFCL consists of parallel- and series-connected superconducting YBCO elements and a limiting resistor. Before designing the SFCL, some tests were carried out. The width and length of each element used in the tests are 30 mm and 210 mm, respectively. The element consists of YBCO thin film of about 200 nm in thickness on cerium dioxide (CeO2) as a cap-layer on a sapphire substrate by metal-organic deposition with a protective metal coat. In the tests, characteristics of each element, such as over-current, withstand-voltage, and so on, were obtained. From these characteristics, series and parallel connections of the elements, called units, were considered. The characteristics of the units were obtained by tests. From the test results, a single phase prototype SFCL was manufactured and tested. Thus, an SFCL rated at 6.6 kV and 400 A can be designed. © 2009 IEEE.

Gone in 0.6 seconds: the encoding of motor memories depends on recent sensorimotor states.

Real-world tasks often require movements that depend on a previous action or on changes in the state of the world. Here we investigate whether motor memories encode the current action in a manner that depends on previous sensorimotor states. Human subjects performed trials in which they made movements in a randomly selected clockwise or counterclockwise velocity-dependent curl force field. Movements during this adaptation phase were preceded by a contextual phase that determined which of the two fields would be experienced on any given trial. As expected from previous research, when static visual cues were presented in the contextual phase, strong interference (resulting in an inability to learn either field) was observed. In contrast, when the contextual phase involved subjects making a movement that was continuous with the adaptation-phase movement, a substantial reduction in interference was seen. As the time between the contextual and adaptation movement increased, so did the interference, reaching a level similar to that seen for static visual cues for delays >600 ms. This contextual effect generalized to purely visual motion, active movement without vision, passive movement, and isometric force generation. Our results show that sensorimotor states that differ in their recent temporal history can engage distinct representations in motor memory, but this effect decays progressively over time and is abolished by ∼600 ms. This suggests that motor memories are encoded not simply as a mapping from current state to motor command but are encoded in terms of the recent history of sensorimotor states.

Effect of laser heating temperature on coating characteristics of Stellite 6 deposited by cold spray

Laser-assisted cold spray (LCS) is a new coating and fabrication process which combines some advantages of CS: solid-state deposition, retain their initial composition and high build rate with the ability to deposit materials which are either difficult or impossible to deposit using cold spray alone. Stellite 6 powder is deposited on medium carbon steels by LCS using N 2 as carrier gas pressure. The topography, cross section thickness, structure of the coatings is examined by SEM, optical microscopy, EDX. The results show that thickness and fluctuation of coating are improved with increased deposition site temperature. Porosity of coating is affected by N 2 and deposition site temperature. In this paper, it presents optimal coating using N 2 at a pressure of 3 MPa and temperature of 450°C and deposition site temperature of 1100°C.

6种常用渔药对厚颌鲂鱼苗的急性毒性试验

在水温29~30℃条件下,用硫酸铜与硫酸亚铁合剂(比例5:2)、高锰酸钾、晶体敌百虫、强氯精、溴氯菊脂、甲醛共6种药物对厚颌鲂(Megalobrama pellegrini)鱼苗进行急性毒性试验。试验结果显示,6种药物48h的半致死浓度分别为5.78、6.50、4.90、2.55、0.042、120mg/L,安全浓度分别为0.445、0.528、0.268、0.190、0.003、7.500mg/L,厚颌鲂鱼苗对药物敏感性比一般鱼类高,实际用药时应根据具体情况确定用药时间和用药量,溴氯菊脂不宜作为治疗药物

Performance comparison of Stellite 6® deposited on steel using supersonic laser deposition and laser cladding

Stellite 6® powders were deposited on carbon steel using Supersonic Laser Deposition. The microstructure and performance of the coatings were examined using SEM, optical microscopy, EDS, XRD, microhardness testing and pin-on-disc wear testing. The results showed that the microstructure and wear behaviour of the most successful SLD deposition conditions with N2 at a pressure of 30bar, a temperature of 450°C and a deposition power of 1.5kW were compared with that of optimised laser cladding. © 2012 Elsevier B.V.