999 resultados para Imunofluorescencia indireta
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Virtual Reality it's present in our personal and professional daily life. Currently it comes been tested in many parameters. An example is, as an intervention mode related to healthy and rehabilitation field ( since phobias to physical problems ) due to dynamic interactive between human being and virtual reality, was able to be designed by a hardware and software integrated system. His appearance was in 1950s, through NASA's flight simulators in order to train astronauts in epoch (TORI et al.,2006).Subsequently, this began to expand to entertainment industry, where due to consumerism, only increasing his capacity to become so much more realist and reliable with the real environment. Because of this, beyond of your capacity be accessible which is low-cost, was used in scientific researches (motor area, physical aptitudes, cognitive, health, among others). The projected interactivity in this system type, in general, seek act according to people answers about the elements of virtual reality. Using the capacity that the reality is able to act of integrated way for human being. Following these concepts about virtual reality, the present study of literature revision, it comes as an purpose see humanity development. Since its birth, going on that point until your actuation and direct and indirect interplay in today's society from maturational form. Taking the concept of researchers' theory like Piaget. Following this line, the study will analyse how virtual reality might come to modify and act in social construction of society nowadays
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Agronomia (Proteção de Plantas) - FCA
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Co-infections by Leishmania (L.) chagasi, Trypanosoma evansi, Toxoplasma gondii and Neospora caninum in dogs were investigated. Amastigotes forms of Leishmania spp. were detected by cytopathological analysis of lymph nodes in 46,42% (39/84) of dogs. In a male dog, adult, without defined breed, from rural area and positive for Leishmania, were observed flagellated forms of T. evansi in blood smear. By immunofluorescence antibody test, 5,95% (5/84) of dogs were considered reactive to T. gondii, with titer equal to or higher than 1:64, while 3,57% (3/84) were reactive to N. caninum, with titer ≥1:50. Among the animals with visceral leishmaniasis, one showed positive serological response to T. gondii and two for N. caninum. All dogs reactive to N. caninum were from rural area and the predominance of infection by T. gondii was in dogs from urban area. A young male dog from the rural area and seropositive for T. gondii showed Ehrlichia spp. morulae in the cytology and positive reaction for canine distemper virus. Thus, further studies are needed to assess the epidemiology of these infections in canine population, especially with respect to the reservoirs of Trypanosoma spp. in rural areas.
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Muscle activity has been studied indirectly through analyses of temperature variation in skin surface temperature on the masticatory muscles. These procedure may be an important tool for clinical evaluation and assessment of the evolution of temporomandibular disorders, as well as monitoring the adopted protocol. Thus, its utilization for identified pathological alterations on blood circulation and/or on metabolic activity in subcutaneous tissues, such as the masticatory muscles, is justified. The aim of this study was to investigate the effect of occlusal splints’ thickness on the variation of surface temperature of the anterior temporal and masseter muscles during rest and clenching. It were analyzed 20 symptomatic subjects (10 males and 10 females) selected through the Research Diagnostic Criteria (RDC) questionnaire. Temperature expressed in degrees Celsius (ºC) was measured on the surface of both muscles measured with the aid of a digital thermometer with an infrared radiation reading system. The surface temperature of the anterior temporal and masseter muscles presented significant reduction during teeth clenching in relation to the mandibular rest position. However, there were no significant differences between conditions with and without occlusal splint or between the different thicknesses of splints analyzed. Masticatory muscles showed a considerable similar behavior for both sides, and no significant distinctions were observed between male and female patients.
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Para avaliar e planejar dietas para indivíduos e grupos populacionais são necessárias estimativas muito acuradas das necessidades e do consumo de energia. O objetivo foi realizar uma revisão bibliográfica das metodologias de determinação das necessidades energéticas para adultos e dos métodos de avaliação do consumo. Em 1918, Harris e Benedict propuseram equações que estimam a necessidade energética basal a partir de calorimetria indireta para indivíduos, depois disso, foram propostas outras equações para o cálculo das necessidades de energia em repouso ou em atividade. Recentemente, a água duplamente marcada é empregada para propor equações mais apropriadas. Contudo, a acurácia destas equações pode ser questionada. Por outro lado, os métodos de avaliação do consumo energético apresentam limitações que demandam cuidado na escolha e aplicação, assim como na interpretação dos resultados. A escolha do método de avaliação depende e pode ser influenciada pela população avaliada, tipo e objetivo do estudo, tempo disponível e do treinamento dos avaliadores. Deste modo, a avaliação do balanço energético está condicionada, tanto às limitações da avaliação através de equações, quanto dos métodos existentes de avaliação do consumo alimentar.
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Obesity is fundamentally a problem of energy balance that develops when the energy intake is greater than the total energy expenditure (TEE). TEE is composed by the sum of resting energy expenditure (REE), thermal effect of foods and thermal effect of physical activity. Many factors can affect energy expenditure and energy homeostasis. Historically, the energy expenditure has been measured by indirect calorimetry. However, more recently, the use of doubly labeled water has allowed the assessment of energy expenditure in a 24 hour period, which expanded the knowledge on energy metabolism. Factors such as gender, age, thyroid disorders, physical activity and body composition affect REE as already established in the literature. However, there has been speculation whether other factors, such as adipocytokines and diet composition, which might be involved in the genesis of obesity by decreasing the REE. So, this paper aims to review the factors that can alter the energy metabolism.
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This study evaluated the potential of congenital transmission in goats experimentally infected and reinfected with Toxoplasma gondii, in three gestational stages (initial, intermediate and final). Of the 25 non-pregnant females negative for T. gondii, 20 were orally inoculated with 2.5 x 103 T. gondii ME49 oocysts. Of these, 15 pregnant females chronically infected were reinoculated, via oral, with 2.5 x 103 T. gondii VEG oocysts. Five experimental groups were formed (n=5): I, II and III (reinoculations in the initial, intermediate and final gestational stage, respectively), IV (inoculation) and V (no inoculation). Clinical and serological exams (IgG IFAT [indirect immunofluorescence antibody test]) in different days of evaluation, and bioassay and PCR were performed in all goats. In the infected goats with T. gondii a peak of 40.2°C (IV) at nine, seroconversion (IgG≥64) at 21 and stabilization (IgG<1024) at 119 days postinoculation were observed. In the reinfected goats with T. gondii occurred an increase in IgG titers (≥1,024) at 28 (I), 7 (II) and 3 (III) days post-reinoculation. During kidding were observed only in the reinfected groups: dystocia, malformation body, stillbirth and weakness, and IgG anti-Toxoplasma were detected in all and in some offsprings of the reinfected and infected goats, respectively. Tissue parasitism by T. gondii was diagnosed by bioassay and PCR in infected and reinfected goats and in their offspring. The congenital toxoplasmosis was possible in goats chronically infected and reinfected with T. gondii. The primary infection with T. gondii did not protect the pregnant goats against congenital disease resulting from toxoplasmic reinfection, in different gestational stages (initial, intermediate and final).
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The ancient struggle for a National Conference on Communication in Brazil, truly representative and with clear opportunities to define new rules to the sector of communication, has been waged for years and was conducted in late 2009. It is proposed to identify the background of the historic struggle for CNC can be perceived in the defense of press freedom and freedom of expression in seventeenth and eighteenth centuries, most recently in the movements of democratization policy in the 80s and 90s. The research was done by analyzing the direct and indirect official document concerning the establishment of guidelines and the convening of the Conference, and manifestation of civil society and business.
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In 2010, the Brazilian forest sector is represented by about 30,000 companies producing US$ 21 billion annually and account for approximately 5% of the gross domestic product (GDP) in the country. The sanding process is highly demanded in various stages of industrialization of the wood, when there is a need for a better quality surface finishing. The objective of this work was to analyze the influence of cutting speed and sandpaper granulometry on both the surface finishing of pieces of Eucalyptus grandis processed through tubular sanding and on the sanding efforts (force and power of sanding). Four cutting speeds were used (19.5, 22.7, 26 and 28.1 m/s), one advance speed (16 m/min) and three sets of sandpaper (80-100, 80-120 and 100-120) being one for chipping and another for finishing, respectively. A central data acquisition system was set up to capture the variables (cutting power, acoustic emission and vibration) in real time. The cutting force was obtained indirectly, through a frequency inverter. The roughness of the parts was measured by a roughness meter before and after sanding. The highest cutting speed used (28.1 m/s) consumed more power and generated more acoustic emission among the four speeds tested. Regarding the vibration, the lower cutting speed (19.5 m/ s) generated the highest vibration in the sander machine. It is concluded that the range of 100-120 sandpapers resulted in values of average roughness (Ra) lower than the other sets of sandpaper used, as it resulted in better surface finishing.