Use of extensible internal device in the femur of young dogs

An extensible internal device (EID) was developed to preserve growth plate during the treatment of fracture complications or segmental bone loss from tumour resection in children. Since this type of extensible, trans-physeal, internal fixation device has only been used in a few paediatric cases; the aim of this study was to evaluate an in vivo canine study, a surgical application of this device, and its interference with longitudinal growth of the non-fractured distal femur. Ton clinically healthy two- to three-month-old poodles weighing 1.5-2.3 kg were used. Following a medial approach to the right distal femur, one extremity of the EID, similar to a T-plate, was fixed in the femoral condyle with two cortical screws placed below the growth plate. The other extremity, consisting of an adaptable brim with two screw holes and a plate guide, was fixed in the third distal of the femoral diaphysis with two cortical screws. The EID was removed 180 days after application. All of the dogs demonstrated full weight-bearing after surgery. The values of thigh and stifle circumferences, and stifle joint motion range did not show any difference between operated and control hindlimbs. The plate slid in the device according to longitudinal bone growth, in all but one dog. In this dog, a 10.5% shortening of the femoral shaft was observed due to a lack of EID sliding. The other dogs had the some longitudinal lengths in both femurs. The EID permits longitudinal bone growth without blocking the distal femur growth plate if appropriately placed.

Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis

In the present study, the validation of an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of canine brucellosis is described. Two different antigenic extracts, obtained by heat or ultrasonic homogenization of microbial antigens from a wild isolate of Brucella canis bacteria, were compared by ELISA and Western blot (WB). A total of 145 canine sera were used to define sensitivity, specificity and accuracy of the ELISA as follows: (1) sera from 34 animals with natural B. canis infection, confirmed by blood culture and PCR, as well as 51 sera samples from healthy dogs with negative results by the agar-gel immunodiffusion (ACID) test for canine brucellosis, were used as the control panel for B. cants infection; and (2) to scrutinize the possibility of cross reactions with other common dog infections in the same geographical area in Brazil, 60 sera samples from dogs harboring known infections by Leptospira sp., Ehrlichia canis, canine distemper virus (CDV), Neospora caninum, Babesia canis and Leishmania chagasi (10 in each group) were included in the study. The ELISA using heat soluble bacterial extract (HE-antigen) as antigen showed the best values of sensitivity (91.18%), specificity (100%) and accuracy (96.47%). In the WB analyses, the HE-antigen showed no cross-reactivity with sera from dogs with different infections, while the B. canis sonicate had various protein bands identified by those sera. The performance of the ELISA standardized with the heat soluble B. canis antigen indicates that this assay can be used as a reliable and practical method to confirm infection by this microorganism, as well as a tool for seroepidemiological studies. (C) 2010 Elsevier Ltd. All rights reserved.

Strong quenching of chlorophyll fluorescence in the desiccated state in three poikilohydric and homoiochlorophyllous moss species indicates photo-oxidative protection on highly light-exposed rocks of a tropical inselberg

The three poikilophydric and homoiochlorophyllous moss species Campylopus savannarum (C. Muell.) Mitt., Racocarpus fontinaloides (C. Muell.) Par. and Ptychomitrium vaginatum Besch. grow on sun-exposed rocks of a tropical inselberg in Brazil subject to regular drying and wetting cycles. Effective photo-oxidative protection in the light-adapted desiccated state in all three species is achieved by a reduction of ground chlorophyll fluorescence, F, to almost zero. Upon rewatering, the kinetics of the recovery of F in air dry cushions to higher values is very fast in the first 5min, but more than 80min are needed until an equilibrium is reached gradually. The kinetics were not different between the three species. The three moss species, have a distinct niche occupation and form a characteristic zonation around soil vegetation islands on the rock outcrops, where C. savannarum and R. fontinaloides form an inner and outer belt, respectively, around vegetation islands and P vaginatum occurs as small isolated cushions on bare rock. However, they were not distinguished by the reduction of F in the dry state and the rewetting recovery kinetics and only slightly different in their photosynthetic capacity. Stable isotope ratios (delta C-13, delta N-15) indicate that liquid films of water limiting diffusion of CO2 are important in determining carbon acquisition and suggest that limitation of CO2 fixation by water films must be more pronounced over time in P vaginatum than in the latter species. This is determined by both the micro site occupied and the form of the moss cushions. (c) 2007 Elsevier GmbH. All rights reserved.

Mobius Sequence in a Girl and Arthrogryposis in her Half-Brother: Distinct Phenotypes Caused By Prenatal Injuries

Mobius sequence is a congenital facial and abducens nerve palsy, frequently associated to abnormalities of extremities. Arthrogryposis multiplex congenital is defined as a congenital fixation of multiple joints seldom of neurogenic origin. Both sequences must have a genetic origin, but usually are sporadic cases related to environmental factors such as drugs exposition and maternal trauma. A 5-year-old girl and a 1-year-old boy were born with Mobius sequence and arthrogryposis multiplex congenital, respectively. During pregnancies, the mother had vaginal bleeding at 7 weeks and used crack (free-based cocaine) in the first trimester, respectively. The girl also has equinovarus talipes and autistic behavior. The boy has arthrogryposis with flexion contractures of the feet and knees. A vascular disruption, due to hemorrhage and cocaine exposure, causing a transient ischemic insult to embryos in a critical period of development may be responsible for distinct phenotypes in these cases.

Fibroelastic Components of the Vesicourethral Junction of Rats in the Aging Process

The vesicourethral junction comprising the vesical trigone, is relevant in setting and positioning of the urinary bladder, along with the vesical neck, fixed by lateral ligaments of the bladder and tendinous arch of the pelvis fascia. Namely, the puboprostatic ligament (men) and the pubovesical (women). The circular set elastic fibers in this junction are important and valuable in the elasticity and plasticity of the area, allowing quick expansion and withdrawal with the flow of urine, and associated to smooth muscle tissue and nerve control form an important collective to maintain urinary continence. The objective of the present study is to describe the elastic system in the vesicouretral junction in relation to aging and its involvement in the states of urinary continence and incontinence, as well as the study of the vesicouretral junction in various age groups while evaluating with electron transmission microscopy. To carry out the study, 12 Wistar rats were used, divided into groups: neonate (4 animals), adult group (4 animals) and aged group (4 animals). Electron transmission microscopy with use of tanic acid technique associated to glutaraldehyde fixation, satisfactorily showed the extreme structural differences between mature elaunin and oxytalan fibers present between intercelular spaces and bundles of collagen fibers. The phases of elastogenesis in neonate animals and degradation of the elastic system of older animals were also evaluated.

Vibrios dominate as culturable nitrogen-fixing bacteria of the Brazilian coral Mussismilia hispida

Taxonomic characterization was performed on the putative N-2-fixing microbiota associated with the coral species Mussismilia hispida, and with its sympatric species Palythoa caribaeorum, P. variabilis, and Zoanthus solanderi, off the coast of Sao Sebastiao (Sao Paulo State, Brazil). The 95 isolates belonged to the Gammaproteobacteria according to the 16S rDNA gene sequences. In order to identify the isolates unambiguously, pyrH gene sequencing was carried out. The majority of the isolates (n = 76) fell within the Vibrio core group, with the highest gene sequence similarity being towards Vibrio harveyi and Vibrio alginolyticus. Nineteen representative isolates belonging to V. harveyi (n = 7), V. alginolyticus (n = 8), V. campbellii (n = 3), and V parahaemolyticus (n = 1) were capable of growing six successive times in nitrogen-free medium and some of them showed strong nitrogenase activity by means of the acetylene reduction assay (ARA). It was concluded that nitrogen fixation is a common phenotypic trait among Vibrio species of the core group. The fact that different Vibrio species can fix N, might explain why they are so abundant in the mucus of different coral species. (C) 2008 Published by Elsevier GmbH.

Influence of organic fertilization on the number of culturable diazotrophic endophytic bacteria isolated from sugarcane

The numbers of culturable diazotrophic endophytic bacteria (CDEB) from roots stems and leaves of sugarcane submitted to organic inorganic or no fertilization were compared In order to determine the size of the N(2) fixing populations the Most Probable Number technique (MPN) was used The quantification of diazotrophic bacteria by using the acetylene reduction assay (ARA) was more accurate than observing the bacterial growth in the vials to confirm N(2) fixing capability the detection of gene nifH was performed on a sample of 105 Isolated bacteria The production of extracellular enzymes involved in the penetration of the plants by the bacteria was also studied The results showed that organic fertilization enhances the number of CDEB when compared with conventional fertilization used throughout the growing season The maximum number of bacteria was detected in the roots Roots and stems presented the greatest number of CDEB in the middle of the cropping season and in leaves numbers varied according to the treatment Using two pairs of primers and two different methods the nifH gene was found in 104 of the 105 tested isolates Larger amounts of pectinase were released by isolates from sugarcane treated with conventional fertilizers (66%) whereas larger amounts of cellulase were released by strains isolated from sugarcane treated with organic fertilizers (80%) (C) 2010 Elsevier Masson SAS All rights reserved

Bone growth around silicon nitride implants - An evaluation by scanning electron microscopy

Silicon nitride has demonstrated to be a potential candidate for clinical applications because it is a non-cytotoxic material and has satisfactory fracture toughness, high wear resistance and low friction coefficient. In this paper, samples of silicon nitride, which were kept into rabbits` tibias for 8 weeks, and the adjacentbone tissue were analysed by scanning electron microscopy in order to verify the bone growth around the implants and the interaction between the implant and the bone. Bone growth occurred mainly in the cortical areas, although it has been observed that the newly bone tends to grow toward the marrow cavity. Differences were observed between the implants installed into distal and proximal regions. In the first region, where the distance between the implant and the cortical bone is greater than in the proximal region, the osteoconduction process was evidenced by the presence of a bridge bone formation toward the implant surface. The results showed that silicon nitride can be used as biomaterial since the newly bone grew around the implants. (c) 2007 Elsevier Inc. All rights reserved.

Orthodontic brackets removal under shear and tensile bond strength resistance tests - a comparative test between light sources

We have investigated if a new LEDs system has enough efficient energy to promote efficient shear and tensile bonding strength resistance under standardized tests. LEDs 470 +/- 10 nm can be used to photocure composite during bracket fixation. Advantages considering resistance to tensile and shear bonding strength when these systems were used are necessary to justify their clinical use. Forty eight human extracted premolars teeth and two light sources were selected, one halogen lamp and a LEDs system. Brackets for premolar were bonded through composite resin. Samples were submitted to standardized tests. A comparison between used sources under shear bonding strength test, obtained similar results; however, tensile bonding test showed distinct results: a statistical difference at a level of 1% between exposure times (40 and 60 seconds) and even to an interaction between light source and exposure time. The best result was obtained with halogen lamp use by 60 seconds, even during re-bonding; however LEDs system can be used for bonding and re-bonding brackets if power density could be increased.

Coating of Cotton Yarn with Poly(vinyl alcohol) and Poly(N-vinyl-2-pyrrolidone) Crosslinked via Ultraviolet Radiation

The coating of cotton fiber is used in the textile industry to increase the mechanical resistance of the yarn and their resistance to vibration, friction, impact, and elongation, which are some of the forces to which the yarn is subjected during the weaving process. The main objective of this study was to investigate the use of synthetic hydrophilic polymers, poly(vinyl alcohol) (PVA), and poly(N-vinyl-2-pyrrolidone) (PVP) to coat 100% cotton textile fiber, with the aim of giving the fiber temporary mechanical resistance. For the fixation of the polymer on the fiber, UV-C radiation was used as the crosslinking process. The influence of the crosslinking process was determined through tensile testing of the coated fibers. The results indicated that UV-C radiation increased the mechanical resistance of the yarn coated with PVP by up to 44% and the yarn coated with PVA by up to 67% compared with the pure cotton yarn, that is, without polymeric coating and crosslinking. This study is of great relevance, and it is important to consider that UV-C radiation dispenses with the use of chemical substances and prevents the generation of toxic waste at the end of the process. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 119: 2560-2567, 2011

Determinação de indicadores de qualidade para avaliação do processo de gestão de pequenas e médias empresas do setor de construção civil habitacional

A escolha do tema ficou circunscrita ao subsetor de construção habitacional, que é caracterizado pela presença de pequenas e médias empresas. A consolidação de grandes empresas neste ramo de atividades tende a ocorrer sobretudo em conjunturas onde através da intervenção estatal são definidos programas habitacionais de grande escala que requerem das empresas participantes de maior volume de capital e o acesso a tecnologias mais sofisticadas. A importância da pequena empresa neste ramo de atividades pode ser notada atraves da experiência dos países da Comunidade Econômica Europeia, que em 1985 de um total de mais de um milhão de empresas de construção, 90% tinham até 10 empregados. Igual situação se verifica nos Estados Unidos da América onde conforme dados de 1965, também 90 % das 875.000 empresas então existentes no país empregavam menos de 10 pessoas (ONU, 1987). De uma maneira geral a importância do tema fica evidente quando se analisa o peso do setor da construção civil na economia nacional, que se situa em torno de 5% do PIB nos países industrializados, enquanto que nos países de industrialização recente, este percentual pode atingir o índice de 7% (PNU, op.cit.). Tal constatação evidencia que a indústria da construção representa o principal item na composição dos investimentos (formação bruta de capital fixo) das contas nacionais de diversos países, apresentando uma participação relativa quase sempre superior a 55% nos países industrializados e 60% nos países de industrialização recente (ONU, op.cit.). No Brasil tal importância se confirma pelo valor adicionado do setor habitacional correspondente a 2,2% do PIB, e numa visão mais abrangente, dentro dos contornos da indústria da construção civil como um todo, representando cerca de 7,3% do PIB (FIBGE, 1988). Quanto a questão da mão-de-obra, em que pese a ausência de dados no Brasil, e bastante visível sua larga influência na economia atuando como um ramo de atividades multiplicador de alocação de pessoal, principalmente a nível de utilização intensiva de mão-de-obra menos qualificada. Enquanto nos EEUU e CEE 90% das empresas constituíam-se de organizações com menos de 10 empregados, no Brasil onde há ausência de dados oficiais a respeito, na pior das hipóteses pode-se estimar que este percentual pode variar entre 50 a 70% do total de empresas, o que não deixa de ser um dado de extrema magnitude. Foram exatamente a expressão destes números, sem considerar o déficit habitacional em torno de 10 milhões de pessoas, um terço da população, moram em condições inadequadas (Exame, 1991), que influenciaram fortemente a escolha do tema da presente proposta de Tese.

A alocação de custos indiretos na indústria de calçados na Região do Vale dos Sinos (RS): estudos de casos

Este trabalho objetivou investigar, através de um estudo exploratório e descritivo, a relação existente entre os procedimentos, técnicas e métodos adotados na alocação de custos indiretos em cinco indústrias de calçados da região do Vale dos Sinos (RS) e aqueles apontados na literatura de custos. Também, buscou-se obter depoimentos dos principais usuá rios da informação de custos das empresas sobre a utilidade da alocação de custos indiretos no processo de gerência. Desta forma, procedeu-se a uma revisão de fundamentos teóricos da Contabilidade de Custos, sob a ótica da Contabilidade Gerencial, a partir do qual delineou-se um Plano de Referência que orientou as etapas da pesquisa. A seguir, apresenta-se a metodologia utilizada, justificando- se as razões de seu emprego, assim como as limitações inerentes a esse tipo de estudo exploratório. Com base nos dados obtidos através de entrevistas, mediante a aplicação de um questionário e análise de documentos, fez-se uma descrição dos mecanismos utilizados pelas empresas es tudadas, na prática da alocação de custos indiretos, bem como re latou-se os depoimentos dos gerentes entrevistado~ sobre a utilidade das alocações de custos indiretos, em relação à necessidade de informações gerenciais como: avaliação de estoques, determinação de resultados, fixação de preços, controle e avaliação de desempenho, planejamento e tomada de decisão.Os resultados obtidos permitiram a análi·se comparativa das práticas adotadas pelas empresas com o referencialtéÕ rico previamente levantado. Também, oportunizou a que se chegasse a importantes conclusões, além de fornecerem subsídios para algumas recomendações e sugestões para futuras pesquisas.

Gerenciamento de riscos de mercado em uma perspectiva corporativa: análise do setor sucroenergético no Brasil

Este trabalho objetiva analisar e contribuir com o gerenciamento de riscos de preços dentro da indústria sucroenergética brasileira, mais especificamente da região Centro-Sul do país, que é a maior processadora de cana-de-açúcar de todo o mundo. Esta indústria tem características peculiares, tanto quanto às formas de comercialização de seus produtos finais – notadamente o açúcar e o etanol – quanto de sua estrutura de custos, onde a cana-de-açúcar representa algo da ordem de 70% do custo total de produção destes produtos. Assim, a proposição aqui implícita passa por uma abordagem específica para o gerenciamento de riscos de preços destas empresas, definindo como principal fator de risco o distanciamento entre os preços das vendas de açúcar travados em mercado organizado de futuros frente aos preços do açúcar apurados dentro da metodologia Consecana e que formam os preços do principal insumo produtivo destas empresas, a cana-de-açúcar. Assim, com uma abordagem empírica é feita uma análise sobre uma amostra de 1.138 ações de fixações de preços ocorridas durante o ano-safra 2012-13, revelando um comportamento de atuação no mercado futuro por parte destas empresas de maximização do valor da firma. Além disso, serão identificados os principais fatores idiossincráticos e sistêmicos que influenciam o retorno que estas empresas obtêm da comercialização de açúcar para o exterior, bem como os fatores que influenciam a tomada de decisão por parte destas empresas.

Uma técnica para modelagem de cenários consistentes na previsão da demanda por energia elétrica

As técnicas qualitativas disponiveis para a modelagem de cenários têm sido reconhecidas pela extrema limitação, evidenciada no principio das atividades do processo, como a fase inicial de concepção. As principais restrições têm sido: • inexistência de uma ferramenta que teste a consistência estrutural interna do modelo, ou pela utilização de relações econômicas com fundamentação teórica mas sem interface perfeita com o ambiente, ou pela adoção de variações binárias para testes de validação; • fixação "a priori" dos possíveis cenários, geralmente classificados sob três adjetivos - otimista, mais provável e pessimista - enviesados exatamente pelos atributos das pessoas que fornecem esta informação. o trabalho trata da utilização de uma ferramenta para a interação entre uma técnica que auxilia a geração de modelos, suportada pela lógica relacional com variações a quatro valores e expectativas fundamentadas no conhecimento do decisor acerca do mundo real. Tem em vista a construção de um sistema qualitativo de previsão exploratória, no qual os cenários são obtidos por procedimento essencialmente intuitivo e descritivos, para a demanda regional por eletricidade. Este tipo de abordagem - apresentada por J. Gershuny - visa principalmente ao fornecimento de suporte metodológico para a consistência dos cenários gerados qualitativamente. Desenvolvimento e estruturação do modelo são realizados em etapas, partindo-se de uma relação simples e prosseguindo com a inclusão de variáveis e efeitos que melhoram a explicação do modelo. o trabalho apresenta um conjunto de relações para a demanda regional de eletricidade nos principais setores de consumo residencial, comercial e industrial bem como os cenários resultantes das variações mais prováveis das suas componentes exógenas. Ao final conclui-se que esta técnica é útil em modelos que: • incluem variáveis sociais relevantes e de dificil mensuração; • acreditam na importância da consistência externa entre os resultados gerados pelo modelo e aqueles esperados para a tomada de decisões; • atribuem ao decisor a responsabilidade de compreender a fundamentação da estrutura conceitual do modelo. Adotado este procedimento, o autor aqui recomenda que o modelo seja validado através de um procedimento iterativo de ajustes com a participação do decisor. As técnicas quantitativas poderão ser adotadas em seguida, tendo o modelo como elemento de consistência.

Contra as Secas: a engenharia e as origens de um planejamento regional no nordeste brasileiro

Droughts surfaced in 1877 as a crucial problem for the birthing Brazilian nation. Engineers, who formed the country's technical and scientific elite, took it upon themselves to study, understand and fight the problem through planned actions of intervention on space. This work, based on proposals and discussions contained in engineering magazines and reports, aims to provide elements for the comprehension of how these systematized actions against droughts, in the Iate nineteenth and early twentieth century, contributed to spatial analysis and the formation of a (then-inexistent) regional and territorial planning discipline in Brazi!. Engineers, by taking up the position of masterminds in the country's modernization, guaranteed for themselves personal economic stability, social prestige and political power. By understanding nature, either as a resource to be exploited or an adversary to national progress, they contributed to the delimitation of the region now known as the Northeast. By seeking to understand the drought phenomenon, they created knowledge about the space they sought to intervene on; by constructing their projects amid political and economical difficulty, they changed the organizational structures of cities and country in the northeast. The proposals for açudes (Iarge water reservoirs) allowed the fixation of population and the resistance against droughts; the roads - railroads and automotive roadways - connected the sertão to the capitais and the coast, speeding up help to the affected populations during droughts and allowing the circulation of goods so as to strengthen the local economies in normal rimes. The adopted practices and techniques, adapted from foreign experience and developed through trial and improvement, were consolidated as an eminently spatial intervention course, even if a theoretical body of regional or territorial planning wasn't formed in Brazil. Regional Planning proper was first applied in the country in the Northeast itself, in the 1950s, based off an economical view of reality in order to achieve development. The engineer's work prior tothat date, however, cannot be dlsconsldered. It was proved that, despite facing financial and political hurdles, engineers had a profound commitment to the problem and intended to act systematically to transform the economical and social relations in the region, in order to be victorious in their struggle against droughts