Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Terapia fotodinâmica e luz branca: aspectos analíticos e perspectivas da viabilidade do tratamento

Photodynamic Therapy (PDT) is a technique used to treat several types of lesions, such as cancer, microbial control, and esthetic dentistry cases. The performance of PDT involves the use of a photosensitizer (PS), which mainly will be located in cancer cells and is irradiated with visible light. This process, when it occurs in the presence of oxygen triggers the formation of reactive oxygen species that are cytotoxic to cells. These species cause cell death and subsequent tumor necrosis. The use of white light as a light source for multispectral Photodynamic Therapy and its consequences to the photodynamic effect is not yet completely established, and therefore there is interest in studying the parameters involved for analyzing the best conditions for applying treatment. The wavelength is crucial to improve the therapeutic effect, since both the optical properties of the biological tissue as the PS depend on these parameters. For FS studied in this work (Photogem®) are most often used wavelengths in the red region, due to their larger penetration depth in biological tissue. Thus, the light source becomes a fundamental aspect, their choice depends on the specific application and is based on the tumor location, light dose to be delivered and FS chosen. Despite all the advantages presented by lasers, the fact of having an emission spectrum essentially monochrome makes only one possible transition possible for the absorption of FS is used. Therefore, more extensive light sources such as light emitting diodes (LED), could be better used in some cases the laser, with the additional advantage of a reduced cost. Therefore, the choice of the white LED comes from an emission spectrum that still wider LED colors defined by allowing greater use of the several absorption bands and with varying depths of operation, according to the wavelength... (Complete abstract click electronic access below)

Proposta de um dispositivo para ajuste automático da altura de uma cama hospitalar

This paper proposes a mechanical device to adapt a simple model of hospital bed used in the Emergency Department at Santa Casa of Guaratinguetá, allowing the bed’s height adjustment with electromechanical drive. This adaptation aims to introduce improvements in the routine work of nurses and reduce ergonomic problems stemming from repetitive strain during the transfer of patients to stretchers and wheelchairs, as well as providing more comfort to the bedridden patient. The device is mainly composed of: an electric induction motor used to drive the engine, a steel spindle with square thread, which allows axial displacement of the bed, a transmission system with pulleys and belt, which transmits power supplied by the motor to the spindle. The mechanism allows the height of the bed is regulated between 400 and 800 mm. The estimated implementation cost of this proposal is lower than the acquisition cost of a commercial hospital bed with the same features, which confirms the feasibility of the proposed solution

Proposta de um dispositivo para adaptação em uma cama hospitalar para ajuste de altura

In hospitals, health professionals may face different situations and problems. By following them, it is remarkable that one of the difficulties is related to the support provided by the facilities to carry out their work. This also affects the quality of treatment offered to patients. One of the points of improvement that could be studied is hospital beds. The Fawler's simplest hospital bed type, used in most Brazilian public hospitals, is designed with an ergonomic average height that offers a default position for analysis and treatment of patients. There is no possibility of adjusting the height of those beds, and hence there is a limitation in this regard since that height is fixed, but the physical structures of the workers and patients may vary widely and there may be a conflict when there are people too large or small involved in this process. Beyond that, studies by experts show that beds with height adjustment decreases the muscular effort and improve the movement quality of the spine of nursing staff, reducing the force on certain vertebrae and also the ergonomic risk. Of course, patient comfort is also affected accordingly. As the beds made with height adjustment are very expensive, this study aims to design a device to adapt the not adjustable beds. The project must be viable from an economic and mechanic standpoint, being able to adapt both old and new simplest Fawler hospital bed models, since their dimensions are similar. The final result shows that it is possible to adapt such beds using the designed concept at a viable cost. Furthermore, this concept could be applied in other bed types or objects that have hollow feet

Influência da luz LED em culturas de células-tronco mesenquimais in-vitro

Stem cells are defined as cells capable of self-renewal and differentiation into specialized cells when submited to external signalings in the enviroment. Among adult stem cells, mesenchymal cells occupy an important position because they can differentiate into mesodermal cells such as osteoblasts, adipocytes and chondrocytes. Cell therapy consists in the use of mesenchymal stem cells (MSC) in the treatment of degenerative diseases and harmed tissue reconstruction. Due to the longstanding and costly procedure for cultivation of MSC, it was proposed the use of low power light sources, such as light emitting diodes (LED), to optimize these factors. Recent works have shown a series of results from the influence of LED light on biological tissues such as increased rate of cell proliferation, increased RNA, DNA and ATP synthesis rate. The purpose of this study is to compare the biomodulator effect of LED light set at wavelengths 630nm ± 10nm and 805nm ± 10nm on the mesenchymal stem cells proliferation. For this, the mesenchymal stem cells culture adopted the procedure used in the Departament of Animal Reproduction and Veterinary Radiology of the Faculty of Veterinary Medicine and Animal Sciences of Botucatu. MSC were obtained from an adult horse bone marrow, and isolated by density gradient separation, with the FICOLL reagent and by centrifugation. The pellet containing the stem cells was removed and these were placed in low glucose DMEM culture medium, containing 10% fetal calf serum and antibiotics. The material was observed daily by inverted microscopy for monitoring the progression of the cells and subsequently the amount of cells were counted in a Neubauer counting chamber. The amount of MSC was obtained by cell culture seeded in 24 wells culture plate and segregated into three distinct groups: Group 1 was irradiated with wavelength set at 630nm ± 10 nm, Group... (Complete abstract click electronic access below)

Gama Câmara CZT: alteração de paradigmas em aquisição de imagens de medicina nuclear

Nuclear medicine is a medical specialty related to imagery that deals with imaging techniques, diagnosis and therapy, allowing observing the physiological state of tissues noninvasively by marking the molecules participating of these physiological processes with radioactive isotopes, thus creating the called radionuclides. The image of a radionuclide is one of the most important applications of radioactivity in nuclear medicine. The equipment’s of nuclear medicine imaging use the principle of radiation detection, turning it into an electrical signal which, through specific algorithms, allows forming tomographic images that provide information about the functional status of organs. New detection systems have been developed for tomographic acquisitions using solid state detectors. These devices use crystals of cadmium zinc telluride (CdZnTe). Some of the advantages of this detector are a significant improvement of signal to noise ratio, the increased spectral and spatial resolution, which in sum, result in greater clarity of the images obtained, opening new perspectives for imaging protocols previously unattainable. In contrast, all other gamma-cameras equipped with vacuum tubes have remained relatively unchanged for nearly fifty years. In these gamma-cameras, the images are obtained using two steps significantly less efficient: the gamma rays are converted to light through a first device, and then the light is converted into an electrical signal through a second device. One of functions the Medical Physicist is related to the quality control of equipment. This control ensures that the information and images provided are true and thus credible to be used in medical reports. To perform this type of analysis the physicist must understand the performance characteristics and operation of all equipment of the department concerned; besides, in the absence of specific legislation, proposing...(Complete abstract click electronic access below)

O brinquedo na educação infantil: algumas reflexões do uso do brinquedo à luz da sociedade disciplinar foucaultiana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A tradução da obra Inferno, de Patrícia Melo, à luz da Linguística de Corpus

Neste artigo, apresentaremos um estudo da tradução da obra Inferno da autora Patrícia Melo, com o título Inferno, por Clifford Landers. Buscamos refletir sobre como são projetados no exterior uma visão da realidade violenta de grandes centros urbanos brasileiros e sobre o quanto o tradutor conseguiu resgatar da nossa sociedade no texto traduzido. Partimos do pressuposto de que o tradutor, consciente ou inconscientemente, usa recursos durante o processo de mediação entre o texto de partida e o texto de chegada, que tornam a leitura da obra traduzida mais fácil. Baker (1996) propõe a investigação de tipos de comportamento linguístico característicos de textos traduzidos. O objetivo desse trabalho é identificar aspectos de normalização presentes na tradução. Para a investigação, recorremos aos Estudos da Tradução Baseados em Corpus (Baker, 1993, 1996; Camargo, 2005, 2007), à Linguística de Corpus (Berber Sardinha, 2004) e aos estudos sobre normalização de Scott (1998). Pode-se observar a ocorrência de mudança de registro de linguagem, omissões, adições, diferenças no comprimento das sentenças e relacionadas a imprecisões de expressões. Espera-se que o presente trabalho possa contribuir para uma maior conscientização das tendências apresentadas pelos tradutores, e para apresentar as possibilidades oferecidas pela intersecção dos Estudos da Tradução Baseados em Corpus e da Linguística de Corpus.

O ensino de expressões idiomáticas à luz da lexicografia pedagógica

In the current context of lexicographical studies, there have been increasing discussions concerning the issue of the relationship between theoretical knowledge of the lexicon and pedagogical practices involved in its teaching. However, in this context, the special lexicon of Portuguese such as neologisms, foreign words, slang and idioms has received little attention from researchers. There are many theoretical studies on each one of these phenomena, but few studies aim at an organization and inclusion of special units in the teaching of Portuguese. For this reason, this article presents a reflection on the teaching of idioms, and, despite the widely held belief that dictionaries are only needed for the teaching of foreign languages, the article suggests the implementation of special dictionaries for the teaching of Portuguese Language in elementary education.

O processo de compreensão de texto de ficção científica na década de 30: Admirável Mundo Novo à luz da teoria semiótica

This paper is result of an analysis of the Brave New World by Aldous Huxley, made based on the perspective of discursive semiotics. For both, the elements of the generative course of meaning were applied, particularly those in works of Barros (2001) and Fiorin (2000). In view of that each of the three levels (elementary, narrative and discourse) of semiotics approach are considered capable of independent descriptions, we sought through the (re) construction of meaning of specific parts of the work and also the relationship of its thematic route with the time it was composed, found that, as a result of this work, the relationships between fiction and reality and thus the relevance of the work of Aldous Huxley

Dispositivo de microdescargas em gases na pressão atmosférica

Neste trabalho foi desenvolvido um dispositivo de microdescargas em gases à pressão atmosférica para geração de plasma a fim de ser utilizado no tratamento de superfícies. O trabalho foi baseado em um estudo de iniciação científica que aconteceu em duas etapas. Na introdução apresentam-se as motivações do estudo, o dispositivo desenvolvido na primeira etapa do trabalho bem como os primeiros resultados e propostas de melhorias. Definem-se os objetivos da segunda etapa, que contemplam modificações no dispositivo de descarga e na fonte de alimentação. Em seguida são explanados os métodos utilizados para confecção do dispositivo, construção da fonte de alimentação e circuito de fotodetecção para observar as descargas. Apresenta-se o modelo e configurações dos experimentos, os resultados obtidos são expostos e debatidos brevemente. Colocam-se as conclusões do trabalho e novas propostas de investigação e melhoramentos para o estudo das microdescargas. Seguem-se os agradecimentos aos envolvidos no projeto e, por fim, a bibliografia utilizada

O dispositivo de juventude e as políticas públicas no Brasil

Pós-graduação em Ciências Sociais - FFC

Possibilidades de atividades de comunicação emocional entre bebês: um estudo à luz da teoria histórico-cultural

Pós-graduação em Educação - FFC

Dispositivo microfluídico de borracha natural (LAB-ON-A-CHIP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Luz, câmera, flashes: uma compreensão sobre a disciplina de prática de ensino de matemática a distância

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)