The comparison of the Butzler medium, filtration technique and their association with isolation of Campylobacter ssp

Do total de 622 amostras de fezes, sendo 200 de suínos, 220 de bezerros e 202 de cães, Campylobacter foi isolado em 178 amostras. Destas, o agente foi identificado em 64 (36%) amostras, isoladas somente em meio seletivo de Butzler (MSB); em 34 (19%) o microorganismo foi isolado a partir da técnica de filtração (TF) e em 80 (45%), através de ambos os procedimentos (MSB+TF). A comparação entre as proporções de positividade, pelas diferentes técnicas, revelou significância (c2 = 9.184; p > 0,001) sendo a MSB (36%) a mais eficiente comparada com a TF (19%) O uso associado de ambos os procedimentos proporcionou a positividade mais elevada de isolamento com 45%.

AGAR MEDIUM FOR HUMAN UREAPLASMA UREALYTICUM

The authors wish to report a simple medium for the isolation and cultivation of Ureaplasma urealyticum starting from clinical materials. This medium induced growth in eleven of the twelve different serotypes of Ureaplasma urealyticum studied.

Nutritional-requirements of Cryptococcus-laurentii in sugar-cane vinasse medium

The growth of the yeast Cryptococcus laurentii in complete broth for yeast and in liquid and semi-solid sugar cane vinasse media supplemented with several nitrogen and phosphorus sources was analysed in order to evaluate its potential utilization as biomass producer from vinasse. The trials were performed in a 1-liter fermentor, at 30-degrees-C, under magnetic agitation for 48 hours, and initial pH 5.0 for the liquid media. The parameters analysed were biomass, protein, and final pH. For the semi-solid media, agitated flasks under rotational agitation for 24 hours, at 30-degrees-C, and initial pH 5.0 were used. The results obtained showed that the yeast did not grow well in liquid sugar cane vinasse media, both supplemented or not, in comparison to the complete broth for yeast. The protein content was also lower in liquid sugar cane vinasse media. The medium pH did not alter markedly during the cultivation. The best results were obtained in semi-solid sugar cane vinasse media with supplementation, probably due to the known polysaccharide production by this species, which allows a better survival to solid substrates.

Antibiosis associated to Lactobacillus acidophilus growth .1. Strain selection and study of an alternative medium using whey

The production of antimicrobial compounds of some strains of Lactobacillus acidophilus has been studied. They have been grown in whey supplemented with soy milk. It has been found that the production of compounds is able to inhibit the growing of both target bacteria analysed: Staphylococcus aureus and Escherichia coli. The results showed a significant variation (p>0.05) depending on the strain of L. acidophilus and on the level of supplementation utilized. Most of the inhibition observed resulted from the presence of the lactic acid produced. It has also been found the production of other antimicrobial compounds showing inhibitory capacity. The action of these compounds was influenced by the substract pH.

Positron-helium scattering at medium energies

We present results for medium-energy elastic, inelastic [transition to He(1s2(1)s), He(1s2(1)p), He(1s3(1)s), and He(1s3(1)p) states], capture [to Ps(1s), Ps(2s), and Ps(2p) states of the positronium (Ps) atom] and total cross sections of positron-helium scattering in the close coupling approach using realistic wave functions.