CYTOGENETIC AND DNA CONTENT IN 6 GENERA OF THE FAMILY CALLICHTHYIDAE (PISCES, SILURIFORMES)

Cytogenetic studies involving conventional Giemsa staining, C-banding analysis and silver staining of NORs were performed on nine species belonging to six genera of the family Callichthyidae. The diploid number ranged from 2n = 44 to 2n = 100, the number of chromosomal pairs with NORs ranged from 1 to 4 and constitutive heterochromatin was mainly distributed in the centromeric and/or pericentromeric position of the chromosomes. The DNA content of erythrocytes from six species studied ranged from 1.18 +/- 0.07 to 2.77 +/- 0.22 pg/nucleus. The extensive variability in karyotypes and in nuclear DNA content detected are in accordance with the initial hypothesis that chromosome rearrangements and polyploidy have played a significant role in the evolutionary history of Callichthyidae.

Study of structural and photoluminescent properties of Ca8Eu2(PO4)(6)O-2

In this work it is presented for the first time the nanostructured hydroxyapatites doped with 0.5, 1.0 and 2.0 wt% of Eu3+ prepared at room temperature by the mechanical alloying technique. X-ray diffraction powder (XRD), infrared (IR) and Raman scattering spectroscopy, scanning electron microscopy (SEM), microhardness measurements as well as luminescent data of Eu3+ were used to investigate the structural and optical properties of these nanomaterials. The electrical and dielectrical analyses were used with the intention of having a better comprehension about the electromagnetic fields in pure and doped hydroxyapatites.

A Family of Autoconnected Transformers for 12-and 18-Pulse Converters-Generalization for Delta and Wye Topologies

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Níveis de proteína bruta e de energia metabolizável para frangas de postura semipesadas de 1 a 18 semanas de idade

The aim of this experiment was to evaluate the performance of commercial layers between 1 and 18 weeks of age submitted to different crude protein (CP) and metabolizable energy (ME) levels. The trial was carried out at the poultry sector of the Department of Animal Science, of the Centro de Ciencias Agrarias, Universidade Federal of Paraiba, Areia-PB, Brazil. Four hundred and thirty-two Lohmann Brown chicks were used at 3 days of age according to a completely randomized design in a 3 x 3 factorial scheme, with three CP levels and three ME levels. In the first phase (1-6 wk), the birds were fed with diets containing three levels of CP: 21, 22 or 23% CP and three levels of ME: 2,900, 3,000 and 3,100 kcal/kg diet. In the second phase (7-12 wk) and in the third phases (13-18 wk), CP levels of feeding were 18, 19 or 20%, and 16, 17 or 18%, respectively, and three ME levels being 2,700, 2,800 or 2,900 kcal ME/kg diet in these two phases. It was not found interaction between CP and ME levels. Based on the results obtained one recommends for the phases from 1 to 6, 7 to 12 and 13 to 18 weeks of age the levels of 21 % of CP and 2900 kcal of ME/kg diets, 20% of CP and 2700 kcal of ME/kg diets and 16% of CP and 2700 kcal of ME/kg diets respectively.

Early loading of single crowns supported by 6-mm-long implants with a moderately rough surface: a prospective 2-year follow-up cohort study

AimTo evaluate prospectively the clinical and radiographic outcomes after 2 years of loading of 6 mm long moderately rough implants supporting single crowns in the posterior regions.Material and methodsForty SLActive Straumann (R) short (6 mm) implants were placed in 35 consecutively treated patients. Nineteen implants, 4.1 mm in diameter, and 21 implants, 4.8 mm in diameter, were installed. Implants were loaded after 6 weeks of healing. Implant survival rate, marginal bone loss and resonance frequency analysis (RFA) were evaluated at different intervals. The clinical crown/implant ratio was also calculated.ResultsTwo out of 40 implants were lost before loading. Hence, the survival rate before loading was 95%. No further technical or biological complications were encountered during the 2-year follow-up. The mean marginal bone loss before loading was 0.34 +/- 0.38 mm. After loading, the mean marginal bone loss was 0.23 +/- 0.33 and 0.21 +/- 0.39 mm at the 1- and 2-year follow-ups. The RFA values increased between insertion (70.2 +/- 9) and the 6-week evaluation (74.8 +/- 6.1). The clinical crown/implant ratio increased with time from 1.5 at the delivery of the prosthesis to 1.8 after 2 years of loading.ConclusionShort implants (6 mm) with a moderately rough surface loaded early (after 6 weeks) during healing yielded high implant survival rates and moderate loss of bone after 2 years of loading. Longer observation periods are needed to draw more definite conclusions on the reliability of short implants supporting single crowns.To cite this article:Rossi F, Ricci E, Marchetti C, Lang NP, Botticelli D. Early loading of single crowns supported by 6-mm-long implants with a moderately rough surface: a prospective 2-year follow-up cohort study.Clin. Oral Impl. Res. 21, 2010; 937-943.doi: 10.1111/j.1600-0501.2010.01942.x.

Isoniazid acetylating phenotype in patients with paracoccidioidomycosis and its relationship with serum sulfadoxin levels, glucose-6-phosphate dehydrogenase and glutathione reductase activities

The authors evaluated the isoniazid acetylating phenotype and measured hematocrit, hemoglobin, glucose-6-phosphate dehydrogenase and glutathione reductase activities plus serum sulfadoxin levels in 39 patients with paracoccidioidomycosis (33 males and 6 females) aged 17 to 58 years. Twenty one (53.84%) of the patients presented a slow acetylating phenotype and 18 (46.16%) a fast acetylating phenotype. Glucose-6-phosphate-dehydrogenase (G6PD) activity was decreased in 5(23.80%) slow acetylators and in 4 (22.22%) fast acetylators. Glutathione reductase activity was decreased in 14 (66.66%) slow acetylators and in 12(66.66%) fast acetylators. Serum levels of free and total sulfadoxin were higher in slow acetylator (p _ 0.02). Analysis of the results permitted us to conclude that serum sulfadoxin levels are related to the acetylator phenotype. Furthermore, sulfadoxin levels were always above 50 μg/ml, a value considered therapeutic. Glutathione reductase deficiency observed in 66% of patients may be related to the intestinal malabsorption of nutrients, among them riboflavin, a FAD precursor vitamin, in patients with paracoceidioidomycosis.

Structure and catalytic mechanism of glucosamine 6-phosphate deaminase from Escherichia coli at 2.1 Å resolution

Background: Glucosamine 6-phosphate deaminase from Escherichia coli is an allosteric hexameric enzyme which catalyzes the reversible conversion of D-glucosamine 6-phosphate into D-fructose 6-phosphate and ammonium ion and is activated by N-acetyl-D-glucosamine 6-phosphate. Mechanistically, it belongs to the group of aldose-ketose isomerases, but its reaction also accomplishes a simultaneous amination/deamination. The determination of the structure of this protein provides fundamental knowledge for understanding its mode of action and the nature of allosteric conformational changes that regulate its function. Results: The crystal structure of glucosamine 6-phosphate deaminase with bound phosphate ions is presented at 2.1 Å resolution together with the refined structures of the enzyme in complexes with its allosteric activator and with a competitive inhibitor. The protein fold can be described as a modified NAD-binding domain. Conclusions: From the similarities between the three presented structures, it is concluded that these represent the enzymatically active R state conformer. A mechanism for the deaminase reaction is proposed. It comprises steps to open the pyranose ring of the substrate and a sequence of general base-catalyzed reactions to bring about isomerization and deamination, with Asp72 playing a key role as a proton exchanger.

Molecular genetic maps of the group 6 chromosomes of hexaploid wheat (Triticum aestivum L. em. Thell.)

Restriction fragment length polymorphism (RFLP) maps of chromosomes 6A, 6B, and 6D of hexaploid wheat (Triticum aestivum L. em. Thell.) have been produced. They were constructed using a population of F 7-8 recombinant inbred lines derived from a synthetic wheat X bread wheat cross. The maps consist of 74 markers assigned to map positions at a LOD ≥ 3 (29 markers assigned to 6A, 24 to 6B, and 21 to 6D) and 2 markers assigned to 6D ordered at a LOD of 2.7. Another 78 markers were assigned to intervals on the maps. The maps of 6A, 6B, and 6D span 178, 132, and 206 cM, respectively. Twenty-one clones detected orthologous loci in two homoeologues and 3 detected an orthologous locus in each chromosome. Orthologous loci are located at intervals of from 1.5 to 26 cM throughout 70% of the length of the linkage maps. Within this portion of the maps, colinearity (homosequentiality) among the three homoeologues is strongly indicated. The remainder of the linkage maps consists of three segments ranging in length from 47 to 60 cM. Colinearity among these chromosomes and other Triticeae homoeologous group 6 chromosomes is indicated and a consensus RFLP map derived from maps of the homoeologous group 6 chromosomes of hexaploid wheat, tetraploid wheat, Triticum tauschii, and barley is presented.

Exigências de energia metabolizável para frangas de postura de 1 a 18 semanas de idade

The trials were conduced to determine the energy requirements of growing pullets from 1 to 18 weeks of age,by means of the factorial method, using the comparative slaughter technique. The energy requirements for maintenance were determined utilizing the following feeding levels: ad libitum, 80% and 60% of ad libitum and sufficient for maintenance. Heat production was determined as a function of metabolizable energy intake (MEI) and the retained energy, which, when extrapolated to zero of ME intake, indicated net energy (NE) for maintenance of 63.6; 71.24 and 76.78 kcal/kg0.75/day, for the periods from 1 to 6, 7 to 12 and 13 to 18 weeks of age, respectively. ME requirements for maintenance were determined by ME intake regression as a function of the retained energy, which, when extrapolated to zero retained energy, resulted in 86.12; 98.95 and 116.24 kcal/kg0.75 per day for the periods from 1 to 6, 7 to 12 and 13 to 18 weeks of age, respectively. ME requirements for weight gain were determined through regression analysis of the energy present in the body as a function of body weight, obtained by means of weekly slaughtering during the period from 1 to 18 weeks old. The regression coefficients of energy present in the body as a function of the body weight were used for the determination of the NE requirements for gain. By taking into account the conversion efficiencies of ME to NE for gain, the requirements for weight gain were: 4.11, 5.78 and 7.32 kcal/g ME for the periods 1 to 6, 7 to 12, and 12 to 18 weeks of age, respectively.

Exigências de proteína para frangas de postura de 1 a 18 semanas de idade

The objective of this experiment was to determine the protein requirements for hen pullets from 1 to 18 weeks of age, by factorial method, using the nitrogen balance and the comparative slaughtering techniques. Protein requirements for maintenance, was obtained by the nitrogen balance technique using four diets with different protein levels (18,9, 4 and 2% of CP) aiming to obtain positive balance, next to zero and negative nitrogen balance. The endogenous nitrogen losses (0.2575 g of N/kg·75/day) was obtained by regression of nitrogen balance (NB) on ingestion nitrogen (IN). The requirements of nitrogen for maintenance was estimated by the intercept of axis X (0.3831 g of N/kg·75/day). The slope of the straight line still supplied the efficiency of N of the diet (67.21%). The regression coefficients of the equation represented the requirements of net nitrogen for weight gain. Considering the conversion efficiencies of nitrogen of the diet into nitrogen for weight gain, the requirements of nitrogen were determined for weight gain of .065, .087g and .090 g of N per gram of weight gain, for the phases 1 to 6, 7 to 12, and 13 to 18 weeks of age, respectively. Based on the results, three equations of prediction of the daily nitrogen requirements were fitted in function of live weight (LW in kg) and daily weight gain (G in g): 1 to 6 weeks N=.3831 x BW·75 + G .065, 7 to 12 weeks N=.3831 × BW·75 + G .087, and 13 to 18 weeks N=.3831 × BW·75 + G .090.

Marginal fit of nickel-chromium copings before and after internal adjustments with duplicated stone dies and disclosing agent.

STATEMENT OF PROBLEM: Despite careful procedures, master stone dies may be damaged during laboratory procedures. The dentist routinely adjusts castings because the marginal fit of casting is not as accurate as on the dies. PURPOSE: This study evaluated the technique of internal adjustment of castings with use of duplicated stone dies and a disclosing agent to improve marginal fit discrepancy. MATERIAL AND METHODS: Thirty-two nickel-chromium copings were fabricated and simulated standard clinical and laboratory procedures with 2 variables: tooth preparation convergence angles of 6 and 18 degrees, with or without internal relief. Master stone dies and their duplicates were selected for coping construction and internal adjustment, respectively. A specimen positioning device was coupled with a Toolmakers microscope to allow reproducibility of measurements. Each coping was evaluated at 8 locations of its marginal perimeter, before and after internal adjustment. RESULTS: Marginal fit discrepancy of copings were significantly reduced with an internal adjustment technique (mean > 52%) for all experimental groups. Tooth preparations with greater convergence and internally relieved castings recorded a better marginal fit. CONCLUSION: The casting internal adjustment technique with use of duplicated stone dies and a disclosing agent substantially reduced marginal fit discrepancy.

Treatment of traumatized permanent incisors with crown and root fractures: A case report

A case report of the treatment of permanent incisors with crown and root fractures is presented. A radiolucent lesion at the fracture lines was treated with calcium hydroxide in the coronal fragments for 18 months. Clinically, the teeth became firm and the radiographic results after 2 years showed healing of the lesion and hard tissue filling in the space at the fracture lines. © Munksgaard, 2001.

The effect of adhesive systems on the pullout strength of a fiberglass-reinforced composite post system in bovine teeth

Purpose: To evaluate the pullout strength of a glass fiber-reinforced composite post (glass FRC) cemented with three different adhesive systems and one resin cement. The null hypothesis was that pullout strengths yielded by the adhesive systems are similar. Materials and Methods: Thirty bovine teeth were selected. The size of the specimens was standardized at 16 mm by sectioning off the coronal portion and part of the root. The specimens were divided into three groups, according to the adhesive system, which were applied following the manufacturers' instructions: G1, ScotchBond Multi-Purpose Plus; G2, Single Bond; G3, Tyrian SPE/One-Step Plus. The glass FRCs (Reforpost) were etched with 37% H3PO4 for 1 min and silanized (Porcelain Primer). Thereafter, they were cemented with the dual resin cement En-Force. The specimens were stored for 24 h, attached to an adapted device, and submitted to the pullout test in a universal testing machine (1 mm/min). The data were submitted to the one-way ANOVA and Tukey's test (α = 0.05). Results: G1 (30.2 ± 5.8 Kgf) displayed the highest pullout strength (p < 0.001) when compared to G2 (18.6 ± 5.8 Kgf) and G3 (14.3 ± 5.8 Kgf), which were statistically similar. Analysis of the specimens revealed that all failures occurred between the adhesive system and the root dentin (pullout of the post cement), regardless of group. Conclusion: The multiple-bottle, total-etch adhesive system provided higher pullout strength of the glass FRC when compared to the single-bottle, total-etch, and single-step self-etching adhesive systems. The null hypothesis was rejected (p < 0.001).