977 resultados para Cultural method
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Projecte de recerca col·laborativa entre professors d’universitat, mestres i membres del Centre de Recursos Pedagògics del Gironés, realitzada durant l'any 2005. L'objectiu ha estat innovar el coneixement del medi natural, social i cultural. Davant l’actual procés de globalització de la societat que ha implicat, entre d’ altres coses, l’arribada de forts corrents migratoris i la presència d’alumnes d’arreu del món a les escoles catalanes, i en especial a les comarques de Girona, es revisa el Coneixement del Medi com una de les vies tradicionals i necessàries de l’adquisició de la identitat, treballant alhora l’acceptació de la diversitat cultural i de punts de vista que aquesta pot aportar. Els objectius fixats pel treball han estat elaborar una proposta didáctica interdisciplinar, oberta i flexible, per a treballar als diferents cicles de l'Educació Infantil i Primària, experimentant-la en diferents escoles; generar la plana web www.coneixmedi.net que recull les experiències i aportacions de les diferents escoles que hi participen i facilita la comunicació entre els alumnes dels centres; valorar els resultats de l'experiència, fent èmfasi en les percepcions dels nens i nenes participants i en la repercusió dins de l'escola i en d'altres centres i finalment validar un model d'innovació basat en la col·laboració i l'ús de recursos compartits. El pla general de funcionament s’ha articulat seguint el cicle bàsic de la recerca-acció. Entre els resultats obtinguts destaquen molt positivament l’elaboració de nou material basat en el treball de la diversitat, el coneixement de les actituds que es donen en els mestres, les famílies o els alumnes davant de la diversitat i l’alt nivell de participació i d’implicació de la major part dels membres del grup en la proposta i els intercanvis d’experiències i de reflexions generades.
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"Vegeu el resum a l'inici del document del fitxer adjunt."
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"Vegeu el resum a l'inici del document del fitxer adjunt."
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"Vegeu el resum a l'inici del document del fitxer adjunt."
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This document includes the results of the research undertaken by the author on the media planning and coverage of the Barcelona'92 and its cultural implications.
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Quantitative method of viral pollution determination for large volume of water using ferric hydroxide gel impregnated on the surface of glassfibre cartridge filter. The use of ferric hydroxide gel, impregnated on the surface of glassfibre cartridge filter enable us to recover 62.5% of virus (Poliomylitis type I, Lsc strain) exsogeneously added to 400 liters of tap-water. The virus concentrator system consists of four cartridge filters, in which the three first one are clarifiers, where the contaminants are removed physically, without significant virus loss at this stage. The last cartridge filter is impregnated with ferric hydroxide gel, where the virus is adsorbed. After the required volume of water has been processed, the last filter is removed from the system and the viruses are recovered from the gel, using 1 liter of glycine/NaOH buffer, at pH 11. Immediately the eluate is clarified through series of cellulose acetate membranes mounted in a 142mm Millipore filter. For the second step of virus concentration, HC1 1N is added slowly to the eluate to achieve pH 3.5-4. MgC1, is added to give a final concentration of 0.05M and the viruses are readsorbed on a 0.45 , porosity (HA) cellulose acetate membrane, mounted in a 90 mm Millipore filter. The viruses are recovered using the same eluent plus 10% of fetal calf serum, to a final volume of 3 ml. In this way, it was possible to concentrate virus from 400 liters of tap-water, into 1 liter in the first stage of virus concentration and just to 3 ml of final volume in a second step. The efficiency, simplicity and low operational cost, provded by the method, make it feasible to study viral pollution of recreational and tap-water sources.
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A method to purify trypanosomastigotes of some strains of Trypanosoma cruzi (Y, CL, FL, F, "Berenice", "Colombiana" and "São Felipe") from mouse blood by using DEAE-cellulose columns was standardized. This procedure is a modification of the Lanham & Godfrey methods and differs in some aspects from others described to purify T. cruzi bloodstream trypomastigotes, mainly by avoidance of prior purifications of parasites. By this method, the broad trypomastigotes were mainly isolated, accounting for higher recoveries obtained with strains having higher percentages of these forms: processing of infected blood from irradiated mice could be advantageous by increasing the recovery of parasites (percentage and/or total number) and elution of more slender trypomastigotes. Trypomastigotes purified by this method presented normal morphology and motility, remained infective to triatomine bugs and mice, showing in the latter prepatent periods and courses parasitemia similar to those of control parasites, and also reproducing the polymorphism pattern of each strain. Their virulence and pathogenicity also remained considerably preserved, the latter property being evaluated by LD 50 tests, mortality rates and mean survival time of inoculated mice. Moreover, these parasites presented positive, clear and peripheral immunofluorescence reaction at titres similar to those of control organisms, thus suggesting important preservation of their surface antigens.
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A method is described which permits to determine in vivo an in a short period of time (4-6 hours) the sensitivity of T. cruzo strains to known active chemotherapeutic agents. By using resistant- and sensitive T. cruzi stains a fairly good correlation was observed between the results obtained with this rapid method (which detects activity against the circulating blood forms) and those obtained with long-term schedules which involve drug adminstration for at least 20 consecutive days and a prolonged period of assessment. This method may be used to characterize susceptibility to active drugs used clinically, provide infomation on the specific action against circulating trypomastigotes and screen active compounds. Differences in the natural susceptibility of Trypanosoma cruzi strains to active drugs have been already reported using different criteria, mostly demanding long-term study of the animal (Hauschka, 1949; Bock, Gonnert & Haberkorn, 1969; Brener, Costa & Chiari, 1976; Andrade & Figueira, 1977; Schlemper, 1982). In this paper we report a method which detects in 4-6 hours the effect of drugs on bloodstream forms in mice with established T. cruzi infections. The results obtained with this method show a fairly good correlation with those obtained by prolonged treatment schedules used to assess the action of drugs in experimental Chagas' disease and may be used to study the sensitivity of T. cruzi strains to active drugs.
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We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.
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Aquest treball es va presentar a l’Assignatura de Campus “Esport, Olimpisme i Societat” del curs 1995/96 i tracta de l'evolució de la programació esportiva a la Televisió de Catalunya des de la seva creació, així com dels orígens i història del Canal 33.
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The ecology of phlebotomine sandflies in an endemic focus of cutaneous leishmaniasis in Northern Venezuela (San Esteban, Carabobo State) was investigated through a year-term study. Three different habitats: viz. a house, a pridomestic area and a sylvatic area, were covered and the species composition, the abundance and occurrence of each species were analyzed in relation to the habitats, catching methods and hour of catching. L. panamensis, L. gomezi and L. ovallesi are the species which bite man, although almost exclusively at night. All of them hide by day and are common in the sylvatic area. Moreover, L. panamensis and L. gomezi successfully approach the house and seem to settle in the peridomestic area. L. shannoni and L. olmeca bicolor also approach and accidentally bite man. L. trinidadensis, L. atroclavata and L. cayennensis are the common non-antrhopophilic species in the area.
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This text was presented at the Symposium “Olympic Arts and Culture Festivals: Recent Experiences and Future Design”, held in Chicago on the 23-24 June 2008. It provides with an analysis of good and bad points of the Cultural Olympiad of Barcelona’92 in order to discover, from that past experience, any lessons for future Cultural Olympiads and Olympic Movement cultural policy in general, as well as to rethink, in a critical way, Catalan cultural policies.
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Este texto fue presentado en el Simposio “Olympic Arts and Culture Festivals: Recent Experiences and Future Design”, celebrado en Chicago del 23 al 24 de junio de 2008. Proporciona un análisis de las luces y sombras de la Olimpiada Cultural de Barcelona’92 con el fin de descubrir, a partir de esa experiencia previa, cualquier lección para las Olimpíadas Culturales futuras y la política cultural del movimiento olímpico en general, así como repensar, de una manera crítica, las políticas culturales catalanas.