Agreed-upon procedures report on the Iowa Sheep and Wool Promotion Board for the period July 1, 2011 through June 30, 2013

Agreed-upon procedures report on the Iowa Sheep and Wool Promotion Board for the period July 1, 2011 through June 30, 2013

Examination report on the City of Remsen, Iowa and the Remsen Municipal Utilities for the period July 1, 2012 through June 30, 2013

Examination report on the City of Remsen, Iowa and the Remsen Municipal Utilities for the period July 1, 2012 through June 30, 2013

Report on a special investigation of the City of Menlo for the period January 1, 2001 through May 31, 2013

Report on a special investigation of the City of Menlo for the period January 1, 2001 through May 31, 2013

Report on a special investigation of the Cerro Gordo County Management Information Systems (MIS) Department for the period January 1, 2008 through July 31, 2013

Report on a special investigation of the Cerro Gordo County Management Information Systems (MIS) Department for the period January 1, 2008 through July 31, 2013

Examination report on the City of Manly, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Manly, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Hamburg, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Hamburg, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Villisca, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Villisca, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the Villisca Municipal Power Plant in Villisca, Iowa for the period January 1, 2013 through December 31, 2013

Examination report on the Villisca Municipal Power Plant in Villisca, Iowa for the period January 1, 2013 through December 31, 2013

Examination report on the City of Ocheyedan, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Ocheyedan, Iowa for the period July 1, 2012 through June 30, 2013

Report on a review of the Central Procurement Enterprise (CPE) of the Iowa Department of Administrative Services for the period July 1, 2009 through March 31, 2013

Report on a review of the Central Procurement Enterprise (CPE) of the Iowa Department of Administrative Services for the period July 1, 2009 through March 31, 2013

Examination report on the City of Ely, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Ely, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Maharishi Vedic City, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Maharishi Vedic City, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Blue Grass, Iowa for the period July 1, 2012 through June 30, 2013

Examination report on the City of Blue Grass, Iowa for the period July 1, 2012 through June 30, 2013

Report on a special investigation of the University of Iowa Athletic Ticket Office and the operations of the Hawkeye Express for the period September 1, 2005 through November 30, 2013

Report on a special investigation of the University of Iowa Athletic Ticket Office and the operations of the Hawkeye Express for the period September 1, 2005 through November 30, 2013

CTF/NF1 transcription factors act as potent genetic insulators for integrating gene transfer vectors.

Gene transfer-based therapeutic approaches have greatly benefited from the ability of some viral vectors to efficiently integrate within the cell genome and ensure persistent transmission of newly acquired transgenes to the target cell progeny. However, integration of provirus has been associated with epigenetic repercussions that may influence the expression of both the transgene and cellular genes close to vector integration loci. The exploitation of genetic insulator elements may overcome both issues through their ability to act as barriers that limit transgene silencing and/or as enhancer-blockers preventing the activation of endogenous genes by the vector enhancer. We established quantitative plasmid-based assay systems to screen enhancer-blocker and barrier genetic elements. Short synthetic insulators that bind to nuclear factor-I protein family transcription factors were identified to exert both enhancer-blocker and barrier functions, and were compared to binding sites for the insulator protein CTCF (CCCTC-binding factor). Gamma-retroviral vectors enclosing these insulator elements were produced at titers similar to their non-insulated counterparts and proved to be less genotoxic in an in vitro immortalization assay, yielding lower activation of Evi1 oncogene expression and reduced clonal expansion of bone marrow cells.