Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro

This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.

Fatty acid profile, carcass and meat quality traits of young Nellore bulls fed crude glycerin replacing energy sources in the concentrate

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Permethrin chronic exposure alters motor coordination in rats: Effect of calcium supplementation and amlodipine

Recently was observed that pyrethroids decrease motor coordination and that calcium channels can be important targets for this effect. To contribute with this observation, this work studied the motor coordination and exploration (using hole-board apparatus), and locomotion (using open-field apparatus) of rats exposed to following treatments: permethrin (PM), PM plus calcium gluconate (CG) and PM plus amlodipine (AML). The results obtained show that CG or AML alone not changed the motor coordination while PM decreases it. CG kept the effect of permethrin; AML, however, decreased the values of permethrin to the control. Locomotor activity and exploration, which could confound results of motor coordination, were not modified by treatments. The concentration of PM in brain tissue was increased by the CG and AML. The neurosomatic index (weight brain/body weight) was increased by the PM and PM + CG. In conclusion, the combined results here obtained indicates that the calcium ion and the channels in which it is involved can be important targets for the toxic effect of pyrethroid insecticide permethrin on motor nerve activity of rats. (c) 2014 Elsevier B.V. All rights reserved.

Concurrent training effect on muscle fibers in Wistar rats

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da poluição atmosférica no sistema respiratório de indivíduos praticantes de exercício físico aeróbio em ambiente aberto e fechado

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência do treinamento resistido funcional sobre a modulação autonômica cardíaca, força muscular, parâmetros cardiorrespiratórios e qualidade de vida em mulheres jovens saudáveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Crude Glycerin associated with starch or fiber-based energy ingredients at two levels of concentrate for beef cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atitudes sociais em relação à inclusão: efeitos da capacitação de professores para ministrar programa informativo aos alunos

Pós-graduação em Educação - FFC

Efeito de um programa de treinamento funcional de curta duração sobre a composição corporal de mulheres na pós-menopausa

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of a continuous rate infusion of butorphanol in isoflurane-anesthetized horses on cardiorespiratory parameters, recovery quality, gastrointestinal motility and serum cortisol concentrations

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Assessment and comparison of the effects of two techniques on hamstring flexibility

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preschool children and excess weight: the impact of a low complexity intervention in public day care centers

Introduction: childhood obesity is a major public health problem, affecting children even at early ages. Objective: to assess the impact of dietary intervention and relatively simple physical activity on the nutritional state of preschoolers. Method: this was an intervention study in public daycare centers targeting children from 2 to 4 years of age, in the State of São Paulo. The sample consisted of 9 daycare centers covering 578 children, with 264 children in the intervention group (IG) and 314 in the comparison group (CG). Intervention was focused on modifications in diet and physical activity, avoiding overloading the routine of daycare centers, for duration of one year. A zBMI score > 1 (zBMI) and < 2 was considered risk of being overweight, and > 2 zBMI was considered excess weight (overweight and obese). Analysis was done by frequency calculations, comparisons of proportions by χ2, mean comparisons by t-student and calculations according to Pearson’s correlation coefficient. Results: IG showed an inverse correlation between the initial zBMI of the children and the difference with the zBMI at the end of the intervention (rP = -0.39, p <0.0001). The mean difference of zBMI of the overweight children in IG between the beginning and the end of the study period was negative (-0.46 z score), indicating weight reduction, while the children in the CG was positive (+0. 17 z score) (p = 0.0037). Conclusion: intervention in diet and physical activity in overweight preschool children in daycare centers could have a favorable impact on the evolution of their nutritional state.

O exercício resistido na mobilidade, flexibilidade, força muscular e equilíbrio de idosos

Introduction: Some losses resulting from aging can be minimized with the practice of resistive exercises. Objective: To analyze the effects of resistive exercises on flexibility, mobility, muscle strength and balance in the elderly. Method: Twenty elderly individuals were divided between an activity group (AG, n=10; 66.1±6.35 years) and a control group (CG, n=10; 65.4±7.54 years). Mobility, flexibility, muscle strength and balance were evaluated. The AG participated in the training for 13 weeks (one-hour sessions, twice a week). The Wilcoxon non-parametric test (intra-group analysis) was used to analyze the data. A significance level of 5% (p<0.05) was adopted. Results: After training, the AG presented better mobility, lower limb muscle strength, and balance, but no difference in flexibility. Conclusion: The present study allows us to conclude that resistive exercises were able to improve mobility, lower limb muscle strength and balance in elderly individuals.