Cruise report: regional assessment of ecosystem condition and stressor impacts along the northwestern Gulf of Mexico Shelf. NOAA Ship 'Nancy Foster' NF-11-07-RACOW (August 8-16, 2011)

This cruise report is a summary of a field survey conducted along a portion of the U.S. continental shelf in northwestern Gulf of Mexico (GOM), at navigable depths along the coastline seaward to the shelf break (~100m) from about 89°30' W to 95°28' W longitude, August 8 – 16, 2011 on NOAA Ship Nancy Foster Cruise NF-11-07-RACOW. Synoptic sampling of multiple ecological indicators was conducted at each of 34 stations throughout these waters using a random probabilistic sampling design. The original study design consisted of 50 stations extending from the Mississippi delta all the way to the U.S./Mexican border, but vessel failures precluded sampling at 16 stations within the western-most portion of the study area. At each station samples were collected for the analysis of benthic community structure and composition; concentrations of chemical contaminants in sediments and target demersal biota; sediment toxicity; nutrient and chlorophyll levels in the water column; and other basic habitat characteristics such as salinity, temperature, dissolved oxygen, turbidity, pH, sediment grain size, and organic carbon content. Other indicators, from a human-dimension perspective, were also recorded, including presence of vessels, oil rigs, surface trash, visual oil sheens in sediments or water, marine mammals, or noxious/oily sediment odors. The overall purpose of the survey was to collect data to assess the status of ecosystem condition and potential stressor impacts throughout the region, based on these various indicators and corresponding management thresholds, and to provide this information as a baseline for determining how such conditions may be changing with time. While sample analysis is still ongoing, some preliminary results and observations are reported here. A final report will be completed once all data have been processed.

濒危植物秦岭冷杉的种实特性和幼苗适应性

　　秦岭冷杉(Abies chensiensis)是松科冷杉属常绿乔木，我国国家二级濒危植物，主要分布在我国的秦岭。迄今为止，国内外有关秦岭冷杉的研究鲜有报道。本文研究了核心分布区和边缘区秦岭冷杉的结实特性、球果和种子的形态变异、种子萌发特性及幼苗的最初生长和适应状况，并通过与同属非濒危物种巴山冷杉相关特性的比较研究，初步探讨了秦岭冷杉的繁殖生物生态学特性, 为全面揭示秦岭冷杉濒危机制奠定基础。 　　与冷杉属其他种相比，核心区秦岭冷杉种子较大，出种量也较高，但空粒率很高，达到88%以上;边缘分布区的空粒率更达到95%以上，反映出极高的败育率。秦岭地区秦岭冷杉结实量和结实率随海拔高度升高而减少;同时，两个海拔高度的对比表明，低海拔秦岭冷杉具有较大的球果和种子，饱满度高。表明在其垂直分布范围内，低海拔地段更适合秦岭冷杉的生长和发育。 　　核心区（即秦岭地区）的秦岭冷杉球果和种子的绝大部分指标均值显著大于边缘区（即神农架地区）。相同海拔条件下，秦岭冷杉绝大部分球果和种子形态指标在地区间、地区内种群间、种群内部均有显著差异，约85%的变异来自地区内个体间和个体内，说明秦岭冷杉的球果和种子形态特征变化受遗传控制更显著。人工林与天然林相比，大部分人工林球果和种子指标均值和变异幅度大于天然林，表明通过人为经营可以改善秦岭冷杉的一些生殖性状。 　　秦岭冷杉具有浅休眠，除经典的冷层积方法可以打破休眠外，适当的物理、化学方法处理，如流动水冲洗、适宜浓度硝酸钾、赤霉酸浸种也可以起到相似的作用。各种促进萌发的方法所得到的最大萌发率间没有显著差异。核心区种子萌发率显著高于边缘区;此外，核心区种子在萌发速度和进程上也要显著高于边缘区，这也表明核心区秦岭冷杉活力更高。同样，由于巴山冷杉较高的饱满度，同等条件下，巴山冷杉种子萌发率约三倍于秦岭冷杉种子。 　　野外幼苗的适应性研究主要通过播种后观测出苗率及幼苗在各种环境条件下的存活率、生长状况来进行。通过一年的观测发现，秦岭冷杉野外种子出苗率平均只有16.9%，一年后幼苗死亡率高于50%。 林窗中、土壤覆盖厚度2~3cm的苗床中种子出苗率最高;较深土壤厚度出苗率低说明秦岭冷杉土壤种子库对更新贡献较小。层积30天种子出苗率高于未处理的种子，但对生长率没有影响。林外苗床中幼苗生长率较高。矿质土壤基质上幼苗生长率明显高于林地中。

Gill-damage in netted Tilapia mossambica Peters

Tilapia mossambica taken with gill-nets are often found with their gills damaged. Gill-filaments may be partly or completely lost; sometimes even the gill-arches are all missing (Plate IA). The operculum is usually undamaged but may have its posteroventral border slightly frayed (Plate IB). For comparison normal fish are shown in Plates IC and ID. Incidence of gill-damage increases rapidly with length of time the nets remain in the water; in the Parakrama Samudra a mere 2-3 hour interval between setting and lifting results in 5 to 20% of the fish being damaged.

Survey of the regional fish trade

Fish export trade,both regional and international, has significantly contributed to uganda's economic growth and to the livehoods of many Ugandans in terms of revenue and employment. while there is substantive information on local and international fish trade on which decsions could be based to appropriately develop the trade,informatrion on regional fish export trade is lacking and where avaliable,it has not been adequately documented. This report contains the findings of the survey on regional fish trade at the border district of West Nile,South/Western and Eastern regions key markets and selected fish supply beaches on Lake Victoria.

Notes on the fish resources of the Bahuda estuary, Orissa, India

Estuaries form an important site for the zoologist as these are the places known to be very rich in food and nutrients and so, become nesting-cum-breeding grounds for many species and also, own good species diversity. Bahuda estuary of Orissa is located at the inter-state border of Orissa and Andhra Pradesh. Explored for many invertebrates, this estuary was not properly investigated for its rich fish fauna. Various surveys at Bahuda estuary revealed that it is an important site of study for the faunal diversity of fishes in the east coast. Forty-eight species belonging to 33 genera under eight orders and 22 families were examined referring standard literature· and methods. Most of the groups of fishes encountered are of commercial importance.

Geometrical properties and turbulent flame speed measurements in stationary premixed V-flames using direct numerical simulation

Three dimensional, fully compressible direct numerical simulations (DNS) of premixed turbulent flames are carried out in a V-flame configuration. The governing equations and the numerical implementation are described in detail, including modifications made to the Navier-Stokes Characteristic Boundary Conditions (NSCBC) to accommodate the steep transverse velocity and composition gradients generated when the flame crosses the boundary. Three cases, at turbulence intensities, u′/sL, of 1, 2, and 6 are considered. The influence of the flame holder on downstream flame properties is assessed through the distributions of the surface-conditioned displacement speed, curvature and tangential strain rates, and compared to data from similarly processed planar flames. The distributions are found to be indistinguishable from planar flames for distances greater than about 17δth downstream of the flame holder, where δth is the laminar flame thermal thickness. Favre mean fields are constructed, and the growth of the mean flame brush is found to be well described by simple Taylor type diffusion. The turbulent flame speed, sT is evaluated from an expression describing the propagation speed of an isosurface of the mean reaction progress variable c̃ in terms of the imbalance between the mean reactive, diffusive, and turbulent fluxes within the flame brush. The results are compared to the consumption speed, sC, calculated from the integral of the mean reaction rate, and to the predictions of a recently developed flame speed model (Kolla et al., Combust Sci Technol 181(3):518-535, 2009). The model predictions are improved in all cases by including the effects of mean molecular diffusion, and the overall agreement is good for the higher turbulence intensity cases once the tangential convective flux of c̃ is taken into account. © 2010 Springer Science+Business Media B.V.

Statistics and modelling of turbulent kinetic energy transport in different regimes of premixed combustion

The statistical behaviour of turbulent kinetic energy transport in turbulent premixed flames is analysed using data from three-dimensional Direct Numerical Simulation (DNS) of freely propagating turbulent premixed flames under decaying turbulence. For flames within the corrugated flamelets regime, it is observed that turbulent kinetic energy is generated within the flame brush. By contrast, for flames within the thin reaction zones regime it has been found that the turbulent kinetic energy decays monotonically through the flame brush. Similar trends are observed also for the dissipation rate of turbulent kinetic energy. Within the corrugated flamelets regime, it is demonstrated that the effects of the mean pressure gradient and pressure dilatation within the flame are sufficient to overcome the effects of viscous dissipation and are responsible for the observed augmentation of turbulent kinetic energy in the flame brush. In the thin reaction zones regime, the effects of the mean pressure gradient and pressure dilatation terms are relatively much weaker than those of viscous dissipation, resulting in a monotonic decay of turbulent kinetic energy across the flame brush. The modelling of the various unclosed terms of the turbulent kinetic energy transport equation has been analysed in detail. The predictions of existing models are compared with corresponding quantities extracted from DNS data. Based on this a-priori DNS assessment, either appropriate models are identified or new models are proposed where necessary. It is shown that the turbulent flux of turbulent kinetic energy exhibits counter-gradient (gradient) transport wherever the turbulent scalar flux is counter-gradient (gradient) in nature. A new model has been proposed for the turbulent flux of turbulent kinetic energy, and is found to capture the qualitative and quantitative behaviour obtained from DNS data for both the corrugated flamelets and thin reaction zones regimes without the need to adjust any of the model constants. © 2010 Springer Science+Business Media B.V.

Venomics and biological analysis of Scatophagus argus argus (Spotted scat) venom isolated in Persian Gulf, extraction and purification of hemolytic protein

Green scat namely as Scatophagus argus argus is a venomous aquarium fish belonging to Scatophagidae family. It can induce painful wounds in injured hand with partial paralysis to whom that touch the spines. Dorsal and ventral rough spines contain cells that produce venom with toxic activities. According to unpublished data collected from local hospitals in southern coastal region of Iran, S. argus is reported as a venomous fish. Envenomation induces clinical symptoms such as local pain, partial paralysis, erythema and itching. In the present study green scat (spotted scat) was collected from Persian Gulf coastal waters. SDS-PAGE indicated 12 distinct bands in the venom ranged between 10-250 KDa. The crude venom had hemolytic activity on human erythrocytes (1%) with an LC100 (Lytic Concentration) of about 1.7 μg. The crude venom can release 813 μg proteins from 0.5% casein. Phospholipase C activity was recorded at 3.125 μg of total venom. Our findings showed that the edematic activity remained over 48 h after injection. The purification of the venom was done by HPLC and 30 peaks were obtained within 80 min but only one peak in 68 min retention time showed hemolytic activity at 90% acetonitril was isolated. The area percentage of the hemolytic protein showed that this hemolytic protein consist of 32 percent of total proteins and its molecular weight was 72 KDa in SDS_PAGE. The results demonstrated that crude venom extracted from Iranian coastal border has different toxic and enzymatic activities.

Generation, flow and utilisation of information on the regional fish trade

The purpose of the study was to provide an in-depth understanding of information generation, flow and utilization within Uganda’s regional fish trade. The study was carried out at district headquarters, border points, landing sites and border markets, involving DFOs, Customs Officials, BMU executives and market managers.

The Nile perch fishery: traditional and emerging fisheries, overfishing and the use of illegal gears on Lake Albert

The fisheries of Lake Albert have come under increasing focus due to several driving forces that have synergistically evolved resulting into concerns from diverse stakeholders. The driving forces include: the commercialization of the fisheries with entry into the value chain of industrial fish processing, a decline in fish stocks especially of the large-size fishes and the emergency of the light - fishing targeting small pelagic fishes. In addition, the assumption by some opinion leaders that light-fishing (use of light) has destroyed the Nile perch fishery of Lake Albert, other factors such as cross-border fishing conflicts, the emergence of oil, an influx of traders in fish-related activities, and the limited regulatory and enforcement regimes for the diverse commercially exploited fish fauna of Lake Albert all require continuous information and action.

The present-day fishery of the Uganda waters of Lake Victoria

The Uganda waters of Lake Victoria comprise an area of 28,500 square kilometres with a shore line of 2,380 kilometres extending from the Uganda/Tanzania border in the west to the Uganda/Kenya border in the east. A large part of the Uganda waters of the lake is less than 60 metres deep, waters deeper than 60 metres being on the eastern side of the lake. Thus the Uganda part of the lake is tilted towards the east. A number of rivers drain into the lake from the north and the River Nile flows out of the lake towards the Mediterranean Sea. The Ssese, Kome, Buvuma and Busoga Islands form a very distinctive feature of the lake. These are perhaps the remaining high hills which survived the drowning of the northern valleys during the formation of the lake. In fact, in T. P. O'Brien's book 'The Prehistoric Uganda Protectorate (1939)', Solmon gives a critical summary of the work on the formation of Lake Victoria and shows that the northern part of the lake has numerous drowned valleys, a feature which provides varying habitats for particular species of fish and which may have an effect on the species composition reflected in the catches in different areas along the northern shore of the lake. It is interesting to note that although Lake Victoria as a whole has a number of rivers draining into it, Halbfass (1923) calculated and found that 76 per cent of the water entering the lake is precipitation on the lake surface.

Effect of flow-geometry on turbulence-scalar interaction in premixed flames

Turbulent combustion of stoichiometric hydrogen-air mixture is simulated using direct numerical simulation methodology, employing complex chemical kinetics. Two flame configurations, freely propagating and V-flames stabilized behind a hot rod, are simulated. The results are analyzed to study the influence of flame configuration on the turbulence-scalar interaction, which is critical for the scalar gradient generation processes. The result suggests that this interaction process is not influenced by the flame configuration and the flame normal is found to align with the most extensive strain in the region of intense heat release. The combustion in the rod stabilized flame is found to be flamelet like in an average sense and the growth of flame-brush thickness with the downstream distance is represented well by Taylor theory of turbulent diffusion, when the flame-brushes are non-interacting. The thickness is observed to saturate when the flame-brushes interact, which is found to occur in the simulated rod stabilized flame with Taylor micro-scale Reynolds number of 97. © 2011 American Institute of Physics.

Design of carbon nanotube fiber microelectrode for glucose biosensing

BACKGROUND: Carbon nanotube (CNT) fiber directly spun from an aerogel has a unique, well-aligned nanostructure (nano-pore and nano-brush), and thus provides high electro-catalytic activity and strong interaction with glucose oxidase enzyme. It shows great potential as a microelectrode for electrochemical biosensors. RESULTS: Cyclic voltammogram results indicate that post-synthesis treatments have great influence on the electrocatalytic activity of CNT fibers. Raman spectroscopy and electrical conductivity tests suggest that fibers annealed at 250 °C remove most of the impurities without damaging the graphite-like structure. This leads to a nano-porous morphology on the surface and the highest conductivity value (1.1 × 10 5 S m -1). Two CNT fiber microelectrode designs were applied to enhance their electron transfer behaviour, and it was found that a design using a 30 nm gold coating is able to linearly cover human physiological glucose level between 2 and 30 mmol L -1. The design also leads to a low detection limit of 25 μmol L -1. CONCLUSIONS: The high performance of CNT fibers not only offers exceptional mechanical and electrical properties, but also provides a large surface area and electron transfer pathway. They consequently make excellent bioactive microelectrodes for glucose biosensing, especially for potential use in implantable devices. © 2011 Society of Chemical Industry.

Automatic object segmentation from calibrated images

This paper addresses the problem of automatically obtaining the object/background segmentation of a rigid 3D object observed in a set of images that have been calibrated for camera pose and intrinsics. Such segmentations can be used to obtain a shape representation of a potentially texture-less object by computing a visual hull. We propose an automatic approach where the object to be segmented is identified by the pose of the cameras instead of user input such as 2D bounding rectangles or brush-strokes. The key behind our method is a pairwise MRF framework that combines (a) foreground/background appearance models, (b) epipolar constraints and (c) weak stereo correspondence into a single segmentation cost function that can be efficiently solved by Graph-cuts. The segmentation thus obtained is further improved using silhouette coherency and then used to update the foreground/background appearance models which are fed into the next Graph-cut computation. These two steps are iterated until segmentation convergences. Our method can automatically provide a 3D surface representation even in texture-less scenes where MVS methods might fail. Furthermore, it confers improved performance in images where the object is not readily separable from the background in colour space, an area that previous segmentation approaches have found challenging. © 2011 IEEE.

A TAXONOMIC REVIEW OF TYLOTOTRITON VERRUCOSUS ANDERSON (AMPHIBIA, CAUDATA, SALAMANDRIDAE)

Generally it has not been recognized that salamanders of two distinctive color morphs currently are assigned to Tylototriton verrucosus Anderson. One form is uniformly dark brown dorsally, with bright orange coloration confined to the ventral edge of the tail; the other has a dark brown to black dorsal ground color with orange dorsolateral warts, an orange vertebral crest, and orange lateral and medial crests on the head. In addition, the limbs and ventrolateral surfaces of the second form have a variable pattern of orange coloration. The brown form occurs in northeastern India, Nepal, northern Burma, Bhutan, northern Thailand, the type locality in extreme western Yunnan, and perhaps in northern Vietnam. The orange-patterned form occurs only in western Yunnan Province, People's Republic of China. The two forms appear to be allopatric but occur close together in the area of the type locality near the Burma border in western Yunnan. There is no evidence of color intergradation in specimens from this region. Analyses of morphometric and meristic characters, however, suggest the possibility of limited genetic exchange between adjacent populations of brown and orange-patterned forms in western Yunnan. The genetic and taxonomic relationships between the two forms is not fully resolved. However, these two highly distinctive forms obviously have evolved along independent trajectories and merit taxonomic recognition. We therefore propose to restrict the concept of Tylototriton verrucosus to the brown form and designate a neotype for that purpose, and we describe a new species to receive the orange-patterned form.