Cinematographic OH-PLIF measurements of two interacting turbulent premixed flames with and without acoustic forcing

This paper describes an experimental investigation into the interactions that occur between two lean turbulent premixed flames stabilised on conical bluff-bodies when they are moved closer together. Cinematographic OH-PLIF measurements were acquired to investigate adjacent flame front interactions as a function of flame separation distance (S). Flame surface density (FSD) and curvature were determined to characterise the unforced flames. Acoustic forcing was then applied to explore the amplitude dependent thermo-acoustic response. Phase-averaged FSD and global heat release measurements in the form of OH * chemiluminescence were obtained for a range of forcing frequencies (f) and amplitudes (A) as a function of S. As the flames were brought closer together the adjacent annular jets were found to merge into a single jet structure. This caused adjacent flame fronts to merge above the wake region between the two flames at a location determined by the jet efflux (flame angle) and S. This region of flame-flame interaction we refer to as 'interacting region'. In the unforced flames, a trend of increasingly negative curvature for decreasing S produced a small net increase in flame surface area via cusp formation. When subjected to acoustic forcing, S-dependent regimes were found in the global heat release response as a function A. The overall trend showed that the occurrence of jet/flame merging reduces the value of A at which non-linear response occurs. In support of previous findings for flames stabilised along shear layers, the phase-averaged FSD showed that the flame dynamics that drive the thermo-acoustic response result from the roll-up of vortices which generate large-scale vortex-flame interactions. Compared with axisymmetric flames, the occurrence of jet merging alters the vortex-flame interactions resulting in an asymmetric contribution to the heat release between the wall and interacting regions. The majority of the heat release was found to occur in the interacting region through the rapid production and destruction of flame surface area. The occurrence of jet merging and large-scale interactions between adjacent flames result in different physical mechanisms that drive the thermo-acoustic response compared with single axisymmetric flames. © 2011.

Sequence context analysis of 8.2 million single nucleotide polymorphisms in the human genome

We analyzed n-mers (n=3-8) in the local environment of 8,249,446 human SNPs and compared their distribution with that in the genome reference sequences. The results revealed that the short sequences, which contained at least one CpG dinucleotide, occurred

Tomographic reconstruction of OH* chemiluminescence in two interacting turbulent flames

The tomographic reconstruction of OH* chemiluminescence was performed on two interacting turbulent premixed bluff-body stabilized flames under steady flow conditions and acoustic excitation. These measurements elucidate the complex three-dimensional (3D) vortex-flame interactions which have previously not been accessible. The experiment was performed using a single camera and intensifier, with multiple views acquired by repositioning the camera, permitting calculation of the mean and phase-averaged volumetric OH* distributions. The reconstructed flame structure and phase-averaged dynamics are compared with OH planar laser-induced fluorescence and flame surface density measurements for the first time. The volumetric data revealed that the large-scale vortex-flame structures formed along the shear layers of each flame collide when the two flames meet, resulting in complex 3D flame structures in between the two flames. With a fairly simple experimental setup, it is shown that the tomographic reconstruction of OH* chemiluminescence in forced flames is a powerful tool that can yield important physical insights into large-scale 3D flame dynamics that are important in combustion instability. © 2013 IOP Publishing Ltd.

铁盐除磷技术机理及铁盐混凝剂的研究进展

综述了铁盐除磷技术的机理与过程,铁盐溶于水后,Fe3+通过溶解和吸水发生强烈水解, 生成Fe2(OH)24+、Fe3(OH)45+、Fe5(OH)96+、Fe5(OH)87+、Fe5(OH)78+、Fe6(OH)126+、Fe7(OH)129+等多核羟基络合物。这些含铁的羟基络合物能有效地降低或消除水体中胶体的(?)电位,使胶体凝聚,再通过沉淀分离将磷去除。合适的pH可以提高磷的去除率,对于正铁离子,最适宜的pH为4．5～5; 对于亚铁离子,则为7～8。阐述了三氯化铁、硫酸亚铁和硫酸铁、复合亚铁、聚氯化铝

污水中8种雌激素化合物的定量测定

采用固相萃取-气相色谱-质谱法(GC-MS)测定污水中辛酚(OP)、壬酚(NP)、双酚A(BPA)、己烯雌酚(DES)、雌酮(E1)、17β-雌二醇(E2)、17α-乙炔雌二醇(EE2)和雌三醇(E3)8种具有雌激素活性的化合物。被测组分的加标回收率为(65.4±4.0)%～(110.0±4.5)%,检出限为1.0～7.5ng/L(相对标准偏差为5.2%～15.6%)。经检测,武汉某城市污水处理厂进水中的目标化合物(除EE2外)浓度为6.5～8954.9ng/L;除EE2和E3外,出水中的浓度为3.2～2

~(14)C标记1,2,7,8-TCDD在鲤体内分布及代谢的初步研究

利用14C标记1,2,7,8-四氯代二苯并二噁英(1,2,7,8Tetrachloro[U—14C]dibenzodioxin,14C1,2,7,8TCDD)初步研究了其在鲤体内的分布和代谢规律。14C1,2,7,8-TCDD溶解于丙酮/植物油中,腹腔暴露。暴露1、2、4、8、12d后取样,肝脏、胆汁、腹腔脂肪等消化制样后用液闪仪测量放射性活度。肝脏和胆汁内的放射性活度同步变化,都是第8d达到峰值后下降。腹腔脂肪内1—2d放射性明显高于随后取样的样品。肝和腹腔脂肪的分布量之比呈现“S”型变化趋势。暴露4d

2,3,7,8-四氯代二苯并二英(TCDD)和苯并芘(B[a]P)对原代培养鲫鱼肝细胞中卵黄蛋白原诱导的影响

通过测定原代培养鲫鱼(Carassius auratus)肝细胞中雌激素受体所介导的卵黄蛋白原(Vtg)生成以及芳香烃受体所介导的CYP1A1基因转录水平的变化, 建立了一种类雌激素体外实验模型. 实验结果表明, Vtg和Vtg mRNA的表达与己烯雌酚(DES)之间均有很好的剂量-效应关系, Vtg和Vtg mRNA均可作为指示类雌激素毒性的生物标志物. TCDD, B[a]P可显著抑制鱼肝细胞中DES诱导的Vtg和Vtg mRNA的表达, 呈明显的抗雌激素效应, 并同时激活了CYP1A1 基因的表达;

2,3,7,8-TCDD对稀有鮈鲫幼体超微结构影响的观察

国家自然科学基金29607004

Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene

In this study, an IL-8 homologue has been cloned and identified from a reptile, Chinese soft-shelled turtle for the first time. The full-length cDNA of turtle IL-8 was 1188 bp and contained a 312 bp open reading frame (ORF) coding for a protein of 104 amino acids. The chemokine CXC domain, which contained Glu-Leu-Arg (ELR) motif and four cysteine residues, was well conserved in turtle IL-8. The 4924 bp genomic DNA of turtle IL-8 contained four exons and three introns. Phylogenetic analysis showed that the amino acid sequence of turtle IL-8 clustered together with birds. RT-PCR analysis showed that turtle IL-8 mRNA was constitutively expressed liver, spleen, kidney, heart, blood and intestine tissues of control turtles. Real-time quantitative PCR analysis further indicated that the turtle IL-8 mRNA expression was apparent in various tissues at 8 h and up-regulated significantly during 8 h-7 d after Aeromonas hydrophila infection. The present studies will help us to understand the evolution of IL-8 molecule and the inflammatory response mechanism in reptiles. (C) 2009 Elsevier Ltd. All rights reserved.

Comparison of 100 Gb/s Ethernet links using PAM-8, multi-pulse, and hybrid CAP-16/QAM-16 modulation schemes

A theoretical study compares 100 Gb/s Ethernet links and finds that multi-pulse and hybrid CAP-16/QAM-16 (PAM-8) schemes support transmission over 10 km (2 km) SMF. Multi-pulse and CAP-16/QAM-16 need 2× the number of arithmetic operations and 7× or 3× the number of filter taps respectively but exhibit reduced power dissipation compared with PAM-8.

Elevated levels of urinary 8-hydroxy-2'-deoxyguanosine in male electrical and electronic equipment dismantling workers exposed to high concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans, polybrominated diphenyl ethers, and polychlorinated biphenyls

To investigate the occupational exposure levels to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs), indoor dust (n = 3) in workshops and hair samples from male workers (n = 64) were collected at two electrical and electronic equipment waste (E-waste) dismantling factories located in the LQ area in east China in July 11-13, 2006. Pre- and postworkshift urines (64 of each) were also collected from the workers to study oxidative damage to DNA using 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker. The concentrations of PCDD/Fs, PCDD/F-WHO-TEQs, PBDEs, PCBs and PCB-WHO-TEQs were (50.0 +/- 8.1) x 10(3), 724.1 +/- 249.6, (27.5 +/- 5.8) x 10(6), (1.6 +/- 0.4) x 10(9), (26.2 +/- 3.0) x 10(3) pg/g dry weight (dw) in dust, and (2.6 +/- 0.6) x 10(3), 42.4 +/- 9.3, (870.8 +/- 205.4) x 10(3), (1.6 +/- 0.2) x 10(6), 41.5 +/- 5.5 pg/g dw in hair, respectively. The homologue and congener profiles in the samples demonstrated that high concentrations of PCDD/Fs, PBDEs, and PCBs were originated from open burning of E-waste. The 8-OHdG levels were detected at 6.40 +/- 1.64 mu mol/mol creatinine in preworkshift urines. However, the levels significantly increased to 24.55 +/- 5.96 mu mol/mol creatinine in postworkshift urines (p < 0.05). Then, it is concluded that there is a high cancer risk originated from oxidative stress indicated by the elevated 8-OHdG levels in the E-waste dismantling workers exposed to high concentrations of PCDD/Fs, PBDEs, and PCBs.

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved.

A solid-phase microextraction fiber coated with diglycidyloxycalix[4]arene yields very high extraction selectivity and sensitivity during the analysis of chlorobenzenes in soil

A novel fiber coated with novel sol-gel (5,11,17,23-tetra-tert-butyl-25,27-dihydroxy-26,28-diglycidyloxycalix[4]arene/hydroxy-terminated silicone oil; diglycidyloxy-C[4]/OH-TSO) was prepared for use with headspace solid-phase microextraction (HS-SPME) combined with gas chromatography (GC) and electron capture detection (ECD), which was applied in order to determine nine chlorobenzenes in soil matrices. Due to the improved fiber preparation, which increases the percentage of calixarene in the coating, the new calixarene fiber exhibits very high extraction selectivity and sensitivity to chlorine-substituted compounds. Various parameters affecting the extraction efficiency were optimized in order to maximize the sensitivity during the chlorobenzene analysis. Interferences from different soil matrices with different characteristics were investigated, and the amount extracted was strongly influenced by the matrix. Therefore, a standard addition protocol was performed on the real soil samples. The linear ranges of detection for the chlorobenzenes tested covered three orders of magnitude, and correlation coefficients > 0.9976 and relative standard deviations (RSD) < 8% were observed. The detection limits were found at sub-ng/g of soil levels, which were about an order of magnitude lower than those given by the commercial poly(dimethylsiloxane) (PDMS) coating for most of the compounds. The recoveries ranged from 64 to 109.6% for each analyte in the real kaleyard soil matrix when different concentration levels were determined over the linear range, which confirmed the reliability and feasibility of the HS-SPME/GC-ECD approach using the fiber coated with diglycidyloxy-C[4]/OH-TSO for the ultratrace analysis of chlorobenzenes in complex matrices.