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Aspects of the hemes and modulation of hydrogen donors in catalases from bovine liver, yeast, and escherichia coli

Catalase is the enzyme which decomposes hydrogen peroxide to water and oxygen. Escherichia coli contains two catalases. Hydroperoxidase I (HPI) is a bifunctional catalase-peroxidase. Hydroperoxidase II (HPII) is only catalytically active toward H202. Expression of the genes encoding these proteins is controlled by different regimes. HPJI is thought to be a hexamer, having one heme d cis group per enzymatic subunit. HPII wild type protein and heme containing mutant proteins were obtained from the laboratory of P. Loewen (Univ. of Manitoba). Mutants constructed by oligonucleotidedirected mutagenesis were targeted for replacement of either the His128 residue or the Asn201 residue in the vicinity of the HPII heme crevice. His128 is the residue thought to be analogous to the His74 distal axial ligand of the heme in the bovine liver enzyme, and Asn201 is believed to be a residue critical to the function of the enzyme because of its role in orienting and interacting with the substrate molecule. Investigation of the nature of the hemes via absorption spectroscopy of the unmodified catalase proteins and their derived pyridine hemochromes showed that while the bovine and Saccharomyces cerevisiae catalase enzymes are protoheme-containing, the HPII wild type protein contains heme d, and the mutant proteins contain either solely protoheme, or heme d-protoheme mixtures. Cyanide binding studies supported this, as ligand binding was monophasic for the bovine, Saccharomyces cerevisiae, and wild type HPII enzymes, but biphasic for several of the HPII mutant proteins. Several mammalian catalases, and at least two prokaryotic catalases, are known to be NADPH binding. The function of this cofactor appears to be the prevention of inactivation of the enzyme, which occurs via formation of the inactive secondary catalase peroxide compound (compound II). No physiologically plausible scheme has yet been proposed for the NADPH mediation of catalase activity. This study has shown, via fluorescence and affinity chromatography techniques, that NADPH binds to the T (Typical) and A (Atypical) catalases of Saccharomyces cerevisiae, and that wild type HPII apparently does not bind NADPH. This study has also shown that NADPH is unlike any other hydrogen donor to catalase, and addresses its features as a unique donor by proposing a mechanism whereby NADPH is oxidized and catalase is protected from inactivation via the formation of protein radical species. Migration of this radical to a position close to the NADPH is also proposed as an adjunct hypothesis, based on similar electron migrations that are known to occur within metmyoglobin and cytochrome c peroxidase when reacted with H202. Validation of these hypotheses may be obtained in appropriate future experiments.

Biosynthesis of benzyliasoquinoline alkaloids

This research was carried out to obtain a convenient route for the synthesis of [7_ 14C]-p-hydroxy benzaldehyde. Section 1 of the thesis includes a route involving intermediates with protecting groups like benzyl and methyl ethers of the phenols. The benzyl ethers afforded the product in relatively better yield. The overall synthesis involves four steps. Section 2 describes the reactions carried out directly on phenols, and a three step pathway is obtained for the synthesis of p-hydroxy benzaldehyde, which was repeated on labelled compounds to obtain [7_14C]p- hydroxy benzaldehyde. The synthesis involves the reaction of p-bromophenol with Cu14CN to yield [7_ 14C]-p-cyano phenol, which is then reduced to the aldehyde by means of a simple and clean photolysis method. The same route was tried out to get 3,4-dihydroxybenzaldehyde and was found to work equally well for the synthesis of this compound. Section 3 deals with the isolation of labelled alkaloids, corydaline, protopine and reticu1ine from [2-3H,1-14C]-dopamine (3H/ 14C ratio = 4) fed Corydalis solida. 3H/14C ratios in the labelled alkaloids were determined. The uncorrected values showed almost 50% loss of 3H relative to 14C in reticuline, and roughly 75% loss of the 3H relative to 14C in corydaline and protopine.

A Brief sketch of the public services of Major Isaac Roach /

Compiled by a committee of ten citizens, Charles Penrose, chairman.

Reflections upon the late correspondence between Mr. Secretary Smith, and Francis James Jackson, esq., Minister Plenipotentiary of His Britannic Majesty /

Originally published in the Federal Republican of Baltimore.

Daniel Shannon fonds, 1816-1822

Lt. Daniel Shannon fl. 1777-1822, was the only son of Susan Drake, granddaughter of Rev. Thomas Drake, eldest brother of Sir Francis Drake, and Captain Daniel Shannon of the Royal Navy. He married Elizabeth Garvey, daughter of Alexander Garvey and Catharine Borden of New Jersey. Lt. Shannon was a Regular in the British Army and on February 12, 1777 he joined the Royal Standard, 5th New Jersey Volunteers. After being arrested and sentenced to hang for spying he was pardoned through the efforts of his mother Susan Drake Shannon who pleaded his case with the Governor. He served under General Cornwallis at the surrender in Virginia in 1781. In 1783 he moved to New Brunswick, Canada where he was reduced to a half-pay ensign in the 2nd Regiment of the Lincoln Militia. He was granted 500 acres of land on the St. Johns River, and on April 1, 1786 his daughter Catharine was born there. The family returned to the United States, residing in Pennsylvania, for a short time. In 1800 Lt. Shannon, with his mother and family, returned to Canada and settled in Stamford Township where he bought 200 acres of land on the Niagara River near the whirlpool. He later served in the Secret Service during the War of 1812 and was stationed at a lookout point on the Niagara River below the falls. In 1806 Shannon’s daughter, Catharine, married Thomas Lundy, fourth son of William Lundy of Stamford Township.

Association between adolescent leisure, peer social capital and academic performance among Canadian youth

Previous research shows discrepant findings between youth leisure programming (before and after school programs, structured summer program, day camp, overnight camp), academic performance and other youth developmental outcomes. Studies underscores the importance of family, community and school social capital in educational success of youth, investigation of peer social capital in the leisure context and academic performance outcomes is limited. This study uses a sample of 10 and 11 year olds (N=1764) from the Canadian National Longitudinal Survey of Children and Youth (NLSCY) Cycle 6, to study the association between youth leisure programming, peer social capital and academic performance. Ordinal logistic regression models consistently showed a positive association between overnight camp and academic performance even after controlling for determinants of health, and measures of family, school and community social capital. Similarly, the measure of peer social capital was positively associated with academic performance. Most importantly, the interaction between overnight camp participation and peer social capital was significantly associated with academic performance. Study findings, highlight overnight camp opportunities and peer social

Documents and facts, relative to military events, during the late war

Boyd's justification of his conduct and complaints about being dropped from the Army list. Inscribed on page [1]: N. Emery, Esq.

Reflections upon the late correspondence between Mr. Secretary Smith, and Francis James Jackson, esq., Minister Plenipotentiary of His Britannic Majesty

Originally published in the Federal Republican of Baltimore. Printed by Benjamin Edes

Admiralty Commissioner's Orders, October 13, 1812.

Sir John Thomas Duckworth (1747-1817) served as Governor and Commander-in-Chief of Newfoundland when the United States declared war against Great Britain in 1812. He effectively prepared for the war by reviving and expanding the militia forces, establishing a signal station to warn of the approach of enemy ships, and strengthening the seaward forces. He resigned the post in December 1812 in order to take a parliamentary seat for New Romney, on the Kent coast. Sir William Domett (1752-1828) and Sir Joseph Sydney (1768-1831) were both naval officers and members of the Board of Admiralty when the United States declared war on Great Britain in 1812. Sir George Warrender, 4th Baronet (1782-1849), served as Lord of the Admiralty from 1812-1822, and as a Member of Parliament for various constituencies in Great Britain from 1807-1832. Sir John Barrow (1764-1848) was secretary of the Admiralty from 1804-1845, with the exception of the period from Feb. 1806-April 1807. He is best known for his support and encouragement of British exploration, especially in West Africa and the Arctic.

Letter written by Napoleon Buonaparte (Bonaparte) to Guillaume Thomas Francois Raynal, June 24, 1790

Translation: Sir, It will probably be difficult for you to remember among the many strangers who annoy you with their admiration of a person to whom you kindly made civilities last year1 during a pleasant conversation about Corsica. I would be grateful if you could take a look at this sketch of his history.2 I present here the first of two letters. If you agree to them, I will send you the end. My brother, whom I recommend not to forget his deputies' commission to escort Paoli to his country,3 and to come and receive a lesson in virtue and humanity, will give them to you. I respectfully your most humble and obedient servant.4 Buonaparte, artillery officer Ajaccio, [Corsica] June 24 the first year of freedom [1790]5 1 Relations between Napoleon and Raynal have begun in 1789, which seems to confirm a confidence to Las Cases ( Memorial of St. Helena , La Pléiade , vol. I, p. 83) . 2 Latest version of history project of Corsica : Letters on Corsica to Abbe Raynal . 3 Joseph is part of a delegation sent by the city of Ajaccio to host Paoli 's return from exile in London . In doing so he has to go through Marseille where Raynal resides. 4 Shipping autograph, National Archives , 400 AP Biography 1. In the first years of Napoleon Bonaparte (1840) , Coston gives a rough version of this letter that it dates from 1786 , which is impossible because Raynal did not return to France that ' in 1787. Published for the first time in the Memories of Lord Holland (1851) , shipping is now kept in the national Archives Napoleons funds . 5 The letter is dated "June 24, the first year of freedom" (the word "freedom" is underlined twice). Given the dates of stay in Corsica Napoleon, it seems that is present in Ajaccio the month of June in 1790.

Scottish Rite (Masonic Order) French Language Collection, 1787-1825, 1907, n.d.

The origins of the Scottish Rite of Freemasonry can be traced to France around 1754, when a Chapter of Claremont was founded in Paris. Initially this chapter had seven degrees, but by 1758 there were twenty-five degrees, known as the Rite of Perfection. In 1761, Stephen Morin was appointed to introduce the Rite into the New World. He began with Kingston, Jamaica and San Domingo. Further establishments were made in New Orleans, LA(1763); Albany, NY (1767); Philadelphia, PA (1782); and Charleston, SC (1783). In order to improve the disorganized state of the degrees in Europe, “Grand Constitutions” were enacted in 1786. These Constitutions formally brought into existence the “Ancient and Accepted Scottish Rite”. None of the degrees of the Scottish Rite would seem to have origins in Scotland. “Scottish” is translated from the French word “Ecossais”, which is found in some of the French titles of some of the degrees of the Rite of Perfection. It is possible that the Scottish connection is a result of the involvement of a Scotsman, Andrew Michael Ramsey, who may have devised some of the degrees.

Administración de proyectos de construcción

Tesis (Maestría en Ciencias de la Administración con Especialidad en Relaciones Industriales) UANL

Evaluación del uso terapeutico del factor de necrosis tumoral, factor de crecimiento epidermico e insulina sobre el proceso de cicatrización en lesiones causadas por la reacción de Shwartzman en la piel de conejos diabéticos

Tesis (Maestría en Ciencias con Especialidad en Inmunología) UANL