Reação de linhagens de soja a mancha bacteriana marrom.

2016

Detecção de Brucella spp. em queijos artesanais pela técnica da reação em cadeia da polimerase

2015

Reação de genótipos de abóbora a Phytophthora capsici.

O objetivo deste trabalho foi avaliar a reação de 24 genótipos de abóbora (acessos, linhagens e cultivares comerciais) à P. capsici.

Reação de genótipos de girassol para resistência a Sclerotinia sclerotiorum em condições de campo na safrinha 2015.

2016

Reação de genótipos de berinjela ao nematóide-das-galhas(Meloidogyne spp).

Este trabalho teve por objetivo identificar possíveis fontes de resistência ao mematoide-das-galhas em genótipos de berinjela.

Processos de secagem da matéria fresca do capim-elefante e do sorgo biomassa visando sua combustão direta.

2016

Reação de genótipos de videira ao cancro bacteriano.

No Submédio do Vale do São Francisco, Nordeste do Brasil, o cancro bacteriano da videira causado pela Xanthomonas campestris pv. viticola ocasiona grandes prejuízos em genótipos suscetíveis. O objetivo foi avaliar à resistência de genótipos de videira quanto ao cancro bacteriano. Dois experimentos (I e II) com 17 e 36 genótipos, respectivamente, com cinco repetições, foram conduzidos em casa de vegetação com temperatura e umidade médias de 30ºC e 70%. As plantas foram inoculadas com suspensão do isolado bacteriano por fricção com gaze umedecidas com solução bacteriana (A570= 6×108 UFC/ml), incubadas e observadas diariamente quanto aos parâmetros pidemiológicos: período de incubação (PI); incidência de folhas com sintomas (INC); incidência de folhas com cancro (IFC); severidade da doença (SEV); e área abaixo da curva de progresso da SEV (AACPSD), calculada como ∑(yi+yi+1)/2dti. O delineamento estatístico foi inteiramente casualizado. Todos os genótipos foram suscetíveis ao patógeno, mas diferiram significativamente entre si (P=0,05). No grupo I destacaram Itália Melhorada e ?Red Globe? com níveis elevados para todos os parámetros, resultado observado no grupo II apenas para Red Globe. No experimento I, Petit Verdot, BRS Cora e Moscato apresentaram os maiores PI e os menores INC, IFC, SEV e AACPSD; já no experimento II, foram 12 genótipos com menores níveis para todas as variáveis, indicando grande potencial para melhoramento genético e manejo integrado. No agrupamento pelo UPGMA (similaridade 60%) formaram-se cinco grupos no experimento I; e seis no II.

Reação de progênies de soja, em 2015, para cancro da haste e podridão radicular de fitóftora.

2016

Reação de variedades de mandioca (Manihot esculenta Crantz) a Xanthomonas axonopodis pv. manihotis.

O presente trabalho teve como objetivo avaliar 26 materiais de mandioca quanto à resistência a bacteriose em casa-de-vegetação. Plantas com 2 a 3 pares de folhas foram inoculadas com dois isolados de Xanthomonas axonopodis pv. manihotis (Xam) através da pulverização da suspensão bacteriana na face inferior das folhas. As avaliações da severidade da doença foram realizadas aos 4, 6, 8, 11, 13 e 15 dias após a inoculação do patógeno. O ensaio foi montado em esquema fatorial 2 x 26 (2 isolados x 26 variedades) e o delineamento foi em blocos casualizados com 3 repetições. Todos os materiais avaliados apresentaram sintomas característicos da bacteriose. No entanto, as variedades EAB 675, Inajá- PA, Orana, 34 Pretinha-3 e Pretona Erecta apresentaram menor severidade da doença, não apresentando diferença significativa quanto a virulência do patógeno. A variedade 37 Pretinha 4 se apresentou como mais suscetível para os dois isolados. Os isolados em estudo apresentaram variabilidade quanto à agressividade, sendo o isolado Xam P.225 o mais virulento.

Reação de etnovariedades de amendoim à mancha preta (Passalora personata) em Rio Branco, Acre.

O amendoim (Arachis hypogaea L.) é importante fonte de proteína para várias aldeias indígenas na Amazônia. Esta pesquisa teve como objetivo avaliar em campo a reação de cinco variedades de amendoim coloridos cultivados na Terra Indígena Kaxinawa de Nova Olinda, situada no rio Envira, Jordão, AC em relação à mancha preta causada por Passalora personata.

Edema pulmonar neurogênico: relato de dois casos

Neurogenic pulmonary edema is a rare and serious complication in patients with head injury. It also may develop after a variety of cerebral insults such as subarachnoid hemorrhage, brain tumors and after epileptic seizures. Thirty six patients with severe head injury and four patients with cerebrovascular insults treated in Intensive Care Unit of HC-UNICAMP from January to September 1995 were evaluated. In this period there were two patients with neurogenic pulmonary edema, one with head injury and other with intracerebral hemorrhage. Diagnosis was made by rapid onset of pulmonary edema, severe hypoxemia, decrease of pulmonary complacence and diffuse pulmonary infiltrations, without previous history of tracheal aspiration or any other risk factor for developement of adult respiratory distress syndrom. In the first case, with severe head trauma, neurogenic pulmonary edema was diagnosed at admission one hour after trauma, associated with severe systemic inflammatory reaction, and good outcome in three days. The second case, with hemorragic vascular insult, developed neurogenic pulmonary edema the fourth day after drenage of intracerebral hematom and died.

The G209A mutation in the alpha-synuclein gene in Brazilian families with Parkinson's disease

A missense G209A mutation of the alpha-synuclein gene was recently described in a large Contursi kindred with Parkinson's disease (PD). The objective of this study is to determine if the mutation G209A of the alpha-synuclein gene was present in 10 Brazilian families with PD. PD patients were recruited from movement disorders clinics of Brazil. A family history with two or more affected in relatives was the inclusion criterion for this study. The alpha-synuclein G209A mutation assay was made using polymerase chain reaction and the restriction enzyme Tsp45I. Ten patients from 10 unrelated families were studied. The mean age of PD onset was 42.7 years old. We did not find the G209A mutation in our 10 families with PD. Our results suggest that alpha-synuclein mutation G209A is uncommon in Brazilian PD families.

Machado-Joseph disease versus hereditary spastic paraplegia: case report

Machado-Joseph disease (MJD) is the most common autosomal dominant spinocerebellar ataxia and presents great phenotypic variability. MJD presenting with spastic paraparesis was recently described in Japanese patients. We report the case of 41-year-old woman with the phenotype of complicated hereditary spastic paraplegia. Her father died at the age of 56 years due to an undiagnosed progressive neurological disease that presented parkinsonism. She had an expanded allele with 66 CAG repeats and a normal allele with 22 repeats in the gene of MJD. MJD should be considered in the differential diagnosis of autosomal dominant complicated HSP. A patient with the phenotype of complicated HSP and relatives with other clinical features of a neurodegenerative disease should raise the suspicion of MJD.

Projeto de um cortador de base para colhedora de cana-de-açúcar utilizando otimização matemática

A base-cutter represented for a mechanism of four bars, was developed using the Autocad program. The normal force of reaction of the profile in the contact point was determined through the dynamic analysis. The equations of dynamic balance were based on the laws of Newton-Euler. The linkage was subject to an optimization technique that considered the peak value of soil reaction force as the objective function to be minimized while the link lengths and the spring constant varied through a specified range. The Algorithm of Sequential Quadratic Programming-SQP was implemented of the program computational Matlab. Results were very encouraging; the maximum value of the normal reaction force was reduced from 4,250.33 to 237.13 N, making the floating process much less disturbing to the soil and the sugarcane rate. Later, others variables had been incorporated the mechanism optimized and new otimization process was implemented .

Caracterização morfoanatômica e histoquímica de Cuphea carthagenensis (Jacq.) J.f. Macbr. (Lythraceae)

Cuphea carthagenensis (Jacq.) J.F. Macbr. is an herb, which occurs preferably in wet places. Amongst other species of the genus, C. carthagenensis is distinguished for its great chemical potential and frequent use in popular medicine. In this study the morphological and anatomical structures were identified, as well as the histochemical characterization was done. Samples of root, stem and leaves were collected from adult plants. This material was processed for anatomical and histochemical analysis in light microscopy and for morphological analysis, in scanning electron microscopy. Important morphological and anatomical considerations were added for C. carthagenensis, such as: the occurrence of aerenchymatous phellem with suberized layers; the types of trichomes present in the vegetative organs, the characterization of secretory trichomes, as well as the secreted substances. The groups of secondary metabolites presents in the root, stem and leaf of C. carthagenensis with more intense histochemical reaction were: proanthocyanidins, phenolic compounds, acids polysaccharides (mucilage especially) and lipids.