La Imagen de Egipto en la novela histórica de Terenci Moix

El trabajo se compone de siete capítulos, los cuales tienen como objetivo esclarecer la imagen de Egipto en la obra de Terenci Moix, particularmente en sus novelas históricas. El primer capítulo trata sobre la teoría de la novela histórica, el segundo, en la comparación entre las constantes de la literatura catalana y castellana del autor, el tercero, en los motivos que lo llevaron a abordar la temática egipcia, el cuarto es un rastreo de esta temática desde los principios de su literatura y los últimos tres capítulos son un análisis histórico-literario a las novelas que tratan sobre los siguiente periodos: Egipto grecorromano, Egipto romántico del siglo XIX y Egipto amárnico.

The viral nature of Toddia França, 1912

Toddia França, 1912 under the light microscope occurs as inclusion corpuscles in the cytoplasm of erythrocytes of cold-blooded vertebrates sometimes accompanied by crystalloid bodies. Its position among the protozoans or the viruses has been discussed by some authors, but remained unclear. To elucidate this problem we studied Toddia from a Brazilian frog (Leptodactylus ocellatus) by electron microscopy. In the cytoplasm of the infected cells we found no protozoan, but rather virus-like particles often hexagonal in outline, averaging 195 nm excluding their two involving membranes, and presenting a central area of variable electron density. Particles at different stages of development were generally found around or on area lighter density than the cytoplasm. which resembled a virus synthesis site. At high magnification, the nuclear or cytoplasmic crystals allied to Toddia resembled the crystalline lattice of the inclusion bodies associated with the polyhedrosis viruses and poxviruses from insects, of the capsules of granulosis viruses and of other protein crystals in ultrathin sections. Cytochemical tests in Toddia corpuscles displayed exclusively the presence of deoxyribonucleic acid. These findings indicate that Toddia is not a protozoan and demonstrate that it is in all probability a viral inclusion corpuscle. Taking into account the nucleic acid type found in its structure (DNA) and the hexagonal shape usually shown in ultrathin sections by its component particles, which have a cytoplasmic site of synthesis and assembly, we tentatively relate Toddia with the so-called "Icosahedral Cytoplasmic Deoxyriboviruses". We believe that the present paper gives the first report of virus-like particles in L. ocellatus.

Catalan's intervals and realizers of triangulations

The Stanley lattice, Tamari lattice and Kreweras lattice are three remarkable orders defined on the set of Catalan objects of a given size. These lattices are ordered by inclusion: the Stanley lattice is an extension of the Tamari lattice which is an extension of the Kreweras lattice. The Stanley order can be defined on the set of Dyck paths of size n as the relation of being above. Hence, intervals in the Stanley lattice are pairs of non-crossing Dyck paths. In a former article, the second author defined a bijection Φ between pairs of non-crossing Dyck paths and the realizers of triangulations (or Schnyder woods). We give a simpler description of the bijection Φ. Then, we study the restriction of Φ to Tamari’s and Kreweras’ intervals. We prove that Φ induces a bijection between Tamari intervals and minimal realizers. This gives a bijection between Tamari intervals and triangulations. We also prove that Φ induces a bijection between Kreweras intervals and the (unique) realizers of stack triangulations. Thus, Φ induces a bijection between Kreweras intervals and stacktriangulations which are known to be in bijection with ternary trees.

Orthogonal systems in finite grahps

To a finite graph there corresponds a free partially commutative group: with the given graph as commutation graph. In this paper we construct an orthogonality theory for graphs and their corresponding free partially commutative groups. The theory developed here provides tools for the study of the structure of partially commutative groups, their universal theory and automorphism groups. In particular the theory is applied in this paper to the centraliser lattice of such groups.

Estudis funcionals i de plegament dels dominis d'activació de les procarboxipeptidases

Els dominis d’activació (ADs) de les procarboxipeptidases de la subfamília A/B sempre han sorprès ja que representen una quarta part del proenzim. S’han realitzat alguns estudis per intentar descobrir-ne alguna possible funció alternativa, però no han estat fructífers. El descobriment de l’elevada velocitat de plegament del domini d’activació de la procarboxipeptidasa A2 humana, (ADA2h), emperò, va portar a proposar la possibilitat de que realitzessin una funció d’assistència al plegament del domini enzimàtic. Posteriorment, l’anàlisi del plegament d’ADA2h a pH baix va revelar la capacitat d’aquest domini per formar fibres amiloides, a més de demostrar que un increment de l’estabilitat proteica podia prevenir la formació d’aquests agregats. La profunda caracterització del plegament d’ADA2h va fer que aquesta proteïna fos un bon model amiloidogènic, de manera que es van proposar un seguit d’experiments que s’han desenvolupat en el present treball per tal de conèixer millor aquest procés. S’han dut a terme estudis cinètics d’agregació per tal de valorar la contribució dels diferents aminoàcids de la seqüència polipeptídica, utilitzant 29 variants puntuals d’ADA2h. Es va eliminar la contribució de l’estabilitat mitjançant la utilització d’urea, i per dicroïsme circular conjuntament amb un aparell de flux detingut, es van obtenir dues velocitats diferents, v1 i v2, que corresponen a la formació d’un intermediari i a la seva reorganització, respectivament. Experiments complementaris utilitzant espectroscòpia d’infraroig (IR) revelaren la reorganització de l’estat natiu (en aquest cas) per a donar la forma agregada. Les cinètiques d’IR van mostrar que ADA2h forma l’estructura _ típica de les fibres amiloides, previ desplegament les seves hèlixs-_. Finalment, s’han realitzat estudis de biocomputació per tal d’esbrinar possibles funcions alternatives dels ADs. Les superposicions estructurals semblen mostrar similaritat dels ADs amb dominis de reconeixement d’RNA (RRM). Aquesta hipòtesi s’ha comprovat experimentalment amb ADA4h, mostrant una dèbil, però existent, unió a RNA.

CD8 kinetically promotes ligand binding to the T-cell antigen receptor.

The mechanism of CD8 cooperation with the TCR in antigen recognition was studied on live T cells. Fluorescence correlation measurements yielded evidence of the presence of two TCR and CD8 subpopulations with different lateral diffusion rate constants. Independently, evidence for two subpopulations was derived from the experimentally observed two distinct association phases of cognate peptide bound to class I MHC (pMHC) tetramers and the T cells. The fast phase rate constant ((1.7 +/- 0.2) x 10(5) M(-1) s(-1)) was independent of examined cell type or MHC-bound peptides' structure. Its value was much faster than that of the association of soluble pMHC and TCR ((7.0 +/- 0.3) x 10(3) M(-1) s(-1)), and close to that of the association of soluble pMHC with CD8 ((1-2) x 10(5) M(-1) s(-1)). The fast binding phase disappeared when CD8-pMHC interaction was blocked by a CD8-specific mAb. The latter rate constant was slowed down approximately 10-fold after cells treatment with methyl-beta-cyclodextrin. These results suggest that the most efficient pMHC-cell association route corresponds to a fast tetramer binding to a colocalized CD8-TCR subpopulation, which apparently resides within membrane rafts: the reaction starts by pMHC association with the CD8. This markedly faster step significantly increases the probability of pMHC-TCR encounters and thereby promotes pMHC association with CD8-proximal TCR. The slow binding phase is assigned to pMHC association with a noncolocalized CD8-TCR subpopulation. Taken together with results of cytotoxicity assays, our data suggest that the colocalized, raft-associated CD8-TCR subpopulation is the one capable of inducing T-cell activation.

Brain Glucose Transport and Phosphorylation Under Acute Insulin-Induced Hypoglycemia in Mice: An 18F-FDG PET Study.

We addressed the questions of how cerebral glucose transport and phosphorylation change under acute hypoglycemia and what the underlying mechanisms of adaptation are. METHODS: Quantitative (18)F-FDG PET combined with the acquisition of real-time arterial input function was performed on mice. Hypoglycemia was induced and maintained by insulin infusion. PET data were analyzed with the 2-tissue-compartment model for (18)F-FDG, and the results were evaluated with Michaelis-Menten saturation kinetics. RESULTS: Glucose clearance from plasma to brain (K1,glc) and the phosphorylation rate constant increased with decreasing plasma glucose (Gp), in particular at a Gp of less than 2.5 mmol/L. Estimated cerebral glucose extraction ratios taking into account an increased cerebral blood flow (CBF) at a Gp of less than 2 mmol/L were between 0.14 and 0.79. CBF-normalized K1,glc values were in agreement with saturation kinetics. Phosphorylation rate constants indicated intracellular glucose depletion at a Gp of less than 2-3 mmol/L. When brain regions were compared, glucose transport under hypoglycemia was lowest in the hypothalamus. CONCLUSION: Alterations in glucose transport and phosphorylation, as well as intracellular glucose depletion, under acute hypoglycemia can be modeled by saturation kinetics taking into account an increase in CBF. Distinct transport kinetics in the hypothalamus may be involved in its glucose-sensing function.

Which prior knowledge? Quantification of in vivo brain 13C MR spectra following 13C glucose infusion using AMARES.

The recent developments in high magnetic field 13C magnetic resonance spectroscopy with improved localization and shimming techniques have led to important gains in sensitivity and spectral resolution of 13C in vivo spectra in the rodent brain, enabling the separation of several 13C isotopomers of glutamate and glutamine. In this context, the assumptions used in spectral quantification might have a significant impact on the determination of the 13C concentrations and the related metabolic fluxes. In this study, the time domain spectral quantification algorithm AMARES (advanced method for accurate, robust and efficient spectral fitting) was applied to 13 C magnetic resonance spectroscopy spectra acquired in the rat brain at 9.4 T, following infusion of [1,6-(13)C2 ] glucose. Using both Monte Carlo simulations and in vivo data, the goal of this work was: (1) to validate the quantification of in vivo 13C isotopomers using AMARES; (2) to assess the impact of the prior knowledge on the quantification of in vivo 13C isotopomers using AMARES; (3) to compare AMARES and LCModel (linear combination of model spectra) for the quantification of in vivo 13C spectra. AMARES led to accurate and reliable 13C spectral quantification similar to those obtained using LCModel, when the frequency shifts, J-coupling constants and phase patterns of the different 13C isotopomers were included as prior knowledge in the analysis.

La revolución energética cubana en la UCLV: ¿un paso en el camino hacia la sostenibilidad?. Parte I: Análisis de los impactos ambientales

La Revolución Energética Cubana (REC) surge de las necesidades de resolver la situación social y económica de Cuba, sumergida en una crisis energética con constantes apagones que limitaban por completo todo desarrollo del país. La REC (2006) se basa en los principios de la autosuficiencia energética, el ahorro y uso racional de la energía, y el incremento de la eficiencia en todos los aspectos. En el presente trabajo se realiza el análisis de la sostenibilidad socio-ambiental de la REC en la Universidad Central Marta Abreu de Las Villas (UCLV), ubicada en Santa Clara (Cuba). La valoración se ha efectuado mediante el análisis de los impactos ambientales y sociales en dos estudios paralelos que se complementan aplicando la técnica de conmensurabilidad débil. El conjunto de los programas de la REC han propiciado mejoras en el suministro eléctrico y en el ahorro energético, disminuyendo en un 44% las emisiones de CO2, a pesar del incremento en la actividad docente y en la flota de equipos. Las medidas adoptadas han incidido en la calidad de la docencia, las condiciones de vida, la concienciación y la participación de la población, dando lugar a un impacto social positivo. De esta manera, el balance global de la REC es positivo ya que ha permitido el desarrollo de la UCLV sin incrementar los impactos ambientales, por lo que se puede considerar un paso en el camino hacia la sostenibilidad socio-ambiental.

La revolución energética cubana en la UCLV: ¿un paso en el camino hacia la sostenibilidad?. Parte II: Análisis de los impactos sociales

La Revolución Energética Cubana (REC) surge de las necesidades de resolver la situación social y económica de Cuba, sumergida en una crisis energética con constantes apagones que limitaban por completo el desarrollo del país. La REC (2006) es un conjunto de medidas basadas en los principios de la autosuficiencia energética, el ahorro y uso racional de la energía, y el incremento de la eficiencia en todos los aspectos. En el presente trabajo se realiza el análisis de la sostenibilidad socio-ambiental de la REC en la Universidad Central Marta Abreu de las Villas (UCLV), ubicada en Santa Clara (Cuba). La valoración se ha efectuado mediante el análisis de los impactos ambientales y sociales en dos estudios paralelos que se complementan aplicando la técnica de conmensurabilidad débil. El conjunto de los programas de la REC han propiciado mejoras en el suministro eléctrico y en el ahorro energético, disminuyendo en un 30% las emisiones de CO2, a pesar del incremento en la actividad docente y en la flota de equipos, en la universidad. Las medidas adoptadas han incidido en la calidad de la docencia, las condiciones de vida, la concienciación y la participación de la población, dando lugar a un impacto social positivo. De esta manera, el balance global de la REC se califica de positivo ya que ha permitido el desarrollo de la UCLV sin incrementar los impactos ambientales, por lo que se puede considerar un paso en el camino hacia la sostenibilidad socio-ambiental.

New inhibitors of Helicobacter pylori urease holoenzyme selected from phage-displayed peptide libraries.

Urease is an important virulence factor for Helicobacter pylori and is critical for bacterial colonization of the human gastric mucosa. Specific inhibition of urease activity has been proposed as a possible strategy to fight this bacteria which infects billions of individual throughout the world and can lead to severe pathological conditions in a limited number of cases. We have selected peptides which specifically bind and inhibit H. pylori urease from libraries of random peptides displayed on filamentous phage in the context of pIII coat protein. Screening of a highly diverse 25-mer combinatorial library and two newly constructed random 6-mer peptide libraries on solid phase H. pylori urease holoenzyme allowed the identification of two peptides, 24-mer TFLPQPRCSALLRYLSEDGVIVPS and 6-mer YDFYWW that can bind and inhibit the activity of urease purified from H. pylori. These two peptides were chemically synthesized and their inhibition constants (Ki) were found to be 47 microM for the 24-mer and 30 microM for the 6-mer peptide. Both peptides specifically inhibited the activity of H. pylori urease but not that of Bacillus pasteurii.

Directional discrepancy in two dimensions

In the present paper, we study the geometric discrepancy with respect to families of rotated rectangles. The well-known extremal cases are the axis-parallel rectangles (logarithmic discrepancy) and rectangles rotated in all possible directions (polynomial discrepancy). We study several intermediate situations: lacunary sequences of directions, lacunary sets of finite order, and sets with small Minkowski dimension. In each of these cases, extensions of a lemma due to Davenport allow us to construct appropriate rotations of the integer lattice which yield small discrepancy.

The Multiple-partners assignment game with heterogeneous sales and multi-unit demands: competitive equilibria

A multiple-partners assignment game with heterogeneous sales and multiunit demands consists of a set of sellers that own a given number of indivisible units of (potentially many different) goods and a set of buyers who value those units and want to buy at most an exogenously fixed number of units. We define a competitive equilibrium for this generalized assignment game and prove its existence by using only linear programming. In particular, we show how to compute equilibrium price vectors from the solutions of the dual linear program associated to the primal linear program defined to find optimal assignments. Using only linear programming tools, we also show (i) that the set of competitive equilibria (pairs of price vectors and assignments) has a Cartesian product structure: each equilibrium price vector is part of a competitive equilibrium with all optimal assignments, and vice versa; (ii) that the set of (restricted) equilibrium price vectors has a natural lattice structure; and (iii) how this structure is translated into the set of agents' utilities that are attainable at equilibrium.

Update of the BIPM comparison BIPM.RI(II)-K1.Cs-134 of activity measurements of the radionuclide 134Cs to include the 2008 results of the BEV (Austria), the 2009 result of the IRA (Switzerland) and the 2010 results of the NMISA (South Africa)

Purine nucleotide pyrophosphotransferase was purified to apparent homogeneity from a culture filtrate of Streptomyces morookaensis. It is a monomeric protein with a molecular weight of 24 000-25 000, and its isoelectric point is 6.9. The enzyme synthesizes purine nucleoside 5'-phosphate (mono, di, or tri) 3'-diphosphates such as pppApp, ppApp, pApp, pppGpp, ppGpp and pppIpp by transferring a pyrophosphoryl group from the 5'-position of ATP, dATP and ppApp to the 3'-position of purine nucleotides. The purified enzyme catalysed the formation of 435 mumol of pppApp and 620 mumol of pppGpp from ATP and GTP per min mg protein under the standard conditions. The enzyme requires absolutely a divalent cation for activity, and optimum pH for the enzyme activity lay above 10 for Mg2+, for Co2+ and Zn2+ from 9 to 9.5, and for Fe2+ from 7.5 to 8. The following Michaelis constants were determined: AMP, 2.78 mM; ADP, 3.23 mM; GMP, 0.89 mM; GDP, 0.46 mM and GTP, 1.54 mM, in the case of ATP donor. The enzyme is inhibited by guanine, guanosine, dGDP, dGTP, N-bromosuccinimide, iodacetate, sodium borate and mercuric acetate.

Increase of a Calcium Independent Transglutaminase Activity in the Erythrocyte during the Infection with Plasmodium falciparum

We have studied the activity of a calcium dependent transglutaminase (EC 2.3.2.13) during the growth of the parasite Plasmodium falciparum inside the infected human erythrocyte. There is only one detectable transglutaminase in the two-cell-system, and its origin is erythrocytic. No activity was detected in preparations of the parasite devoid of erythrocyte cytoplasm. The Michaelis Menten constants (Km) of the enzyme for the substrates N'N'dimethylcaseine and putrescine were undistinguishable whether the cell extracts used in their determination were obtained from normal or from infected red cells. The total activity of transglutaminase in stringently synchronized cultures, measured at 0.5mM Ca2+, decreased with the maturation of the parasite. However, a fraction which became irreversibly activated and independent of calcium concentration was detected. The proportion of this fraction grew with maturation; it represented only 20% of the activity in 20 hr-old-trophozoites while in 48-hr-schizonts it was more than 85% of the total activity. The activation of this fraction of transglutaminase did not depend on an increase in the erythrocyte cytoplasmic calcium, since most of the calcium was shown to be located in the parasite.