A novel bio-kinematic encoder for human exercise representation and decomposition - Part 1 : Indexing and modelling

Current bio-kinematic encoders use velocity, acceleration and angular information to encode human exercises. However, in exercise physiology there is a need to distinguish between the shape of the trajectory and its execution dynamics. In this paper we propose such a two-component model and explore how best to compute these components of an action. In particular, we show how a new spatial indexing scheme, derived directly from the underlying differential geometry of curves, provides robust estimates of the shape and dynamics compared to standard temporal indexing schemes.

A participatory action research project at the nexus of health promotion and sustainability in higher education

Like many educational institutions, Deakin University has embraced the challenge of developing curricula to ensure that graduates are environmentally literate and competent to address sustainability in professional practice. Despite an abundance of literature pertaining to the link between human health and environmental degradation, the development of health-related education for sustainability curriculum has been slow. Health promotion, an integral aspect of health professional training in Australia, is considered an area of practice well suited to the action of sustainability. This article highlights the findings of a pilot project that explored which graduate-level health promotion competencies and principles for practice can be transferred to action on sustainability. Methodologically, this study offers a participatory action research process enhanced with case study design principles. Findings from the four case studies highlight that health promotion competencies are compatible with action on sustainability. The article also illuminates the themes in the literature about the value of mutually reinforcing pedagogies associated with education for sustainability and work-integrated learning. The article contributes to discussion in an emerging area of health curriculum, namely, health-related education for sustainability. It posits that health promotion is an area of the health curriculum that can support the development of competencies at the nexus between health and sustainability.

Acting on job stress - do we have a context for action?

Psychosocial risk is possibly the single biggest cause of occupational ill-health inAustralia, causing up to 30% of cardiovascular disease in working men and up to 30% ofdepression in working women. While the number of studies on effective workplaceinterventions has increased significantly in recent years, there has been at best onlylimited analysis examining the context for these interventions. The literature provideslittle evidence with which to answer critical public policy questions. In order to determine how diverse stakeholders are responding to job stress, this studydirectly sought to characterise this context. Through interviews across industry and withkey stakeholders, this study provides a thorough and empirically grounded description ofcurrent Victorian practice, a critical support for developing a systems approach toworkplace stress. The interviews examined the views of Victorian stakeholders in thearea of job stress to investigate understanding of and receptivity to systems approaches and reviewed experiences in workplaces. The picture that emerges from the interview data is contrasting, but with common features across groups. Most parties understood stress as an individual health issue, even though the links to the wider workplace environment were recognised by many. The views of some interviewees imply moral judgements about acceptable stress, experienced by “good” people who deal with trauma and conflict in their work, and unacceptable stress, experienced by “bad” people who can’t cope with the ups and downs of working life. Even so, the need to deal with job stress is recognised by all.

Hydroxyoctadecadienoic acids regulate apoptosis in human THP-1 cells in a PPARγ-dependent manner

Macrophage apoptosis, a key process in atherogenesis, is regulated by oxidation products, including hydroxyoctadecadienoic acids (HODEs). These stable oxidation products of linoleic acid (LA) are abundant in atherosclerotic plaque and activate PPARγ and GPR132. We investigated the mechanisms through which HODEs regulate apoptosis. The effect of HODEs on THP-1 monocytes and adherent THP-1 cells were compared with other C18 fatty acids, LA and α-linolenic acid (ALA). The number of cells was reduced within 24 hours following treatment with 9-HODE (p < 0.01, 30 μM) and 13 HODE (p < 0.01, 30 μM), and the equivalent cell viability was also decreased (p < 0.001). Both 9-HODE and 13-HODE (but not LA or ALA) markedly increased caspase-3/7 activity (p < 0.001) in both monocytes and adherent THP-1 cells, with 9-HODE the more potent. In addition, 9-HODE and 13-HODE both increased Annexin-V labelling of cells (p < 0.001). There was no effect of LA, ALA, or the PPARγ agonist rosiglitazone (1μM), but the effect of HODEs was replicated with apoptosis-inducer camptothecin (10μM). Only 9-HODE increased DNA fragmentation. The pro-apoptotic effect of HODEs was blocked by the caspase inhibitor DEVD-CHO. The PPARγ antagonist T0070907 further increased apoptosis, suggestive of the PPARγ-regulated apoptotic effects induced by 9-HODE. The use of siRNA for GPR132 showed no evidence that the effect of HODEs was mediated through this receptor. 9-HODE and 13-HODE are potent—and specific—regulators of apoptosis in THP-1 cells. Their action is PPARγ-dependent and independent of GPR132. Further studies to identify the signalling pathways through which HODEs increase apoptosis in macrophages may reveal novel therapeutic targets for atherosclerosis.

Specific recognition of the HIV-1 genomic RNA by the Gag precursor

During assembly of HIV-1 particles in infected cells, the viral Pr55(Gag) protein (or Gag precursor) must select the viral genomic RNA (gRNA) from a variety of cellular and viral spliced RNAs. However, there is no consensus on how Pr55(Gag) achieves this selection. Here, by using RNA binding and footprinting assays, we demonstrate that the primary Pr55(Gag) binding site on the gRNA consists of the internal loop and the lower part of stem-loop 1 (SL1), the upper part of which initiates gRNA dimerization. A double regulation ensures specific binding of Pr55(Gag) to the gRNA despite the fact that SL1 is also present in spliced viral RNAs. The region upstream of SL1, which is present in all HIV-1 RNAs, prevents binding to SL1, but this negative effect is counteracted by sequences downstream of SL4, which are unique to the gRNA.

Short-chain fatty acids increase expression and secretion of stromal cell-derived factor-1 in mouse and human pre-adipocytes

Objective: Stromal cell-derived factor-1 (SDF-1) is expressed in pre-adipocytes but its role is unknown. We investigated butyrate (a histone deacetylase inhibitor - HDACi) and other short-chain fatty acids (SCFA) in the regulation of SDF-1. We further investigated whether effects of SCFA were signalled through G protein-coupled receptors FFA2 and FFA3. Design and Results: SDF-1 mRNA expression and protein secretion were studied in 3T3-L1 cells and human pre-adipocytes. SDF-1 was abundant, with mRNA and protein levels increased by butyrate. This was replicated with acetate and propionate, but not with trichostatin or valproate. Trichostatin inhibited SDF-1 secretion. Pertussis toxin blocked stimulation by butyrate. The order of potency of SCFA in stimulating SDF-1 (C3 > C4 > C2) is consistent with action through FFA3. Silencing the FFA3 gene abolished butyrate-stimulated SDF-1 expression and secretion. FFA3 was expressed in both pre-adipocytes and adipocytes, while FFA2 was expressed in adipocytes only. SDF-1 expression was low in murine macrophage J774.2 cells, while the SDF-1 receptor CXCR4 was absent from 3T3-L1 cells but abundant in J774.2 macrophages. In human pre-adipocytes, FFA3 was also expressed and SCFA increased SDF-1 secretion. Conclusions: SDF-1 and CXCR4 may mediate the interaction between adipose stromal cells and macrophages. Effects of SCFA are mediated through FFA3, but not histone deacetylase inhibition.

Cellular localization and associations of the major lipolytic proteins in human skeletal muscle at rest and during exercise

Lipolysis involves the sequential breakdown of fatty acids from triacylglycerol and is increased during energy stress such as exercise. Adipose triglyceride lipase (ATGL) is a key regulator of skeletal muscle lipolysis and perilipin (PLIN) 5 is postulated to be an important regulator of ATGL action of muscle lipolysis. Hence, we hypothesized that non-genomic regulation such as cellular localization and the interaction of these key proteins modulate muscle lipolysis during exercise. PLIN5, ATGL and CGI-58 were highly (>60%) colocated with Oil Red O (ORO) stained lipid droplets. PLIN5 was significantly colocated with ATGL, mitochondria and CGI-58, indicating a close association between the key lipolytic effectors in resting skeletal muscle. The colocation of the lipolytic proteins, their independent association with ORO and the PLIN5/ORO colocation were not altered after 60 min of moderate intensity exercise. Further experiments in cultured human myocytes showed that PLIN5 colocation with ORO or mitochondria is unaffected by pharmacological activation of lipolytic pathways. Together, these data suggest that the major lipolytic proteins are highly expressed at the lipid droplet and colocate in resting skeletal muscle, that their localization and interactions appear to remain unchanged during prolonged exercise, and, accordingly, that other post-translational mechanisms are likely regulators of skeletal muscle lipolysis.

A bayesian nonparametric framework for activity recognition using accelerometer data

Monitoring daily physical activity of human plays an important role in preventing diseases as well as improving health. In this paper, we demonstrate a framework for monitoring the physical activity levels in daily life. We collect the data using accelerometer sensors in a realistic setting without any supervision. The ground truth of activities is provided by the participants themselves using an experience sampling application running on mobile phones. The original data is discretized by the hierarchical Dirichlet process (HDP) into different activity levels and the number of levels is inferred automatically. We validate the accuracy of the extracted patterns by using them for the multi-label classification of activities and demonstrate the high performances in various standard evaluation metrics. We further show that the extracted patterns are highly correlated to the daily routine of users.

Glucose enhancement of recognition memory: differential effects on effortful processing but not aspects of 'remember-know' responses

The administration of a glucose drink has been shown to enhance cognitive performance with effect sizes comparable with those from pharmaceutical interventions in human trials. In the memory domain, it is currently debated whether glucose facilitation of performance is due to differential targeting of hippocampal memory or whether task effort is a more important determinant. Using a placebo-controlled, double-blind, crossover 2(Drink: glucose/placebo) × 2(Effort: ± secondary task) design, 20 healthy young adults' recognition memory performance was measured using the 'remember-know' procedure. Two high effort conditions (one for each drink) included secondary hand movements during word presentation. A 25 g glucose or 30 mg saccharine (placebo) drink was consumed 10 min prior to the task. The presence of a secondary task resulted in a global impairment of memory function. There were significant Drink × Effort interactions for overall memory accuracy but no differential effects for 'remember' or 'know' responses. These data suggest that, in some circumstances, task effort may be a more important determinant of the glucose facilitation of memory effect than hippocampal mediation. This article is part of a Special Issue entitled 'Cognitive Enhancers'.

Doing the right thing at the right time: Assessing responses to patient deterioration in electronic simulation scenarios using course-of-action analysis

International studies indicate that the recognition and management of deteriorating patients in hospitals are poor and that patient assessment is often inadequate. Face-to-face simulation programs have been shown to have an impact on educational and clinical outcomes; however, little is known about performance in contemporary healthcare e-simulation approaches. Using data from an open-access Web-based patient deterioration program (FIRSTACTWeb), the performance of 367 Australian nursing students in identification of treatment priorities and clinical actions was analyzed using a military model of Course of Action Simulation Analysis. Participants' performance in the whole program demonstrated a significant improvement in knowledge and skills (P ≤ .001) with high levels of participant satisfaction. Course of Action Simulation Analysis modeling identified three key participant groupings within which only 18% took the "best course of action" (the right actions and timing), with most (70%) completing the right actions but in the wrong order. The remaining 12% produced incomplete assessments and actions in an incorrect sequence. Contemporary approaches such as e-simulation do enhance educational outcomes. Measurement of performance when combined with Course of Action Simulation Analysis becomes a useful tool in the description of outcomes, an understanding of decision making, and the prediction of future events.

Short communication: bovine-derived proteins activate STAT3 in human skeletal muscle in vitro

Bovine milk contains biologically active peptides that may modulate growth and development within humans. In this study, targeted bovine-derived proteins were evaluated for their effects on signal transducer and activator of transcription-3 (STAT3) phosphorylation in human skeletal muscle cells. Following an acute exposure, bovine-derived acidic fibroblast growth factor-1 (FGF) and leukemia inhibitory factor (LIF) activated STAT3 in differentiating myotubes. Chronic exposure to FGF and LIF during the proliferative phase reduced myoblast proliferation and elevated MyoD and creatine kinase (CKM) mRNA expression without altering apoptotic genes. In mature myotubes, neither FGF nor LIF elicited any action. Together, these data indicate that a reduction in proliferation in the presence of bovine-derived FGF or LIF may stimulate early maturation of myoblasts.

Cultural diversity through transformative action research: translating ideas into real world impact

According to the World Bank, in 2013 more than 215 million people were living outside their countries of birth, and the United Nations Population Fund outlined that if all international migrants lived in the same place they would constitute the world’s fifth most populous country. In other words, the world’s migrant population is greater than at any other time in history and is expected to grow further. As a result of this ever-increasing human mobility, cultural diversity is a fait accompli. For this reason the mission of the Alfred Deakin Research Institute for Citizenship and Globalisation (ADRI-CG), since its inception in 2001, has been to work towards fostering intercultural understanding, human rights and social inclusion through transformative action research and within multidisciplinary approaches. Further, the Institute is mindful that academic work alone is not enough to effect lasting change in both policy and practice, and as such continually seeks strategic partnerships and effective dissemination strategies to influence public policy, and reach communities both locally and globally.

O movimento negro e a Secretaria Especial de Políticas de Promoção da Igualdade Racial: um estudo sobre a luta por sentidos sobre a igualdade racial no Brasil

Esta dissertação tem como objetivo compreender o relacionamento do Movimento Negro e Estado brasileiro no processo de criação da Secretaria Especial de Políticas de Promoção da Igualdade Racial (SEPPIR). Pretendemos jogar luz sobre a relação entre Movimento Negro e Estado na constante luta pelo sentido e significado da desigualdade racial. Será que a constituição de uma Secretaria, com status de Ministério de Estado, é capaz de promover mudanças na visão de desigualdade racial institucionalizada pelo Estado brasileiro? Nosso estudo busca entender como o conflito sobre o sentido da desigualdade racial é incorporado às Políticas Públicas. Utilizamos a categoria analítica Movimento Social para compreender o Movimento Negro, identificando alguns frames que orientam a sua ação. Evidenciamos que estes frames se relacionam na constituição do lugar (entendido como uma série de ligações, nas quais os sentidos das relações sociais são construídos, onde há disputas de poder sobre esses sentidos) da SEPPIR. A ação do Movimento Negro coloca em conflito os sentidos institucionalizados pelo Estado, que se utiliza da cooptação para desmobilizar o Movimento. Ao discutirmos a relação entre Movimento e Estado, relacionamos os frames identificados com a naturalização da desigualdade racial. Essa versão institucionalizada atribui principalmente ao nosso passado escravocrata a causa dessa desigualdade, não apontando para a compreensão do papel do racismo na manutenção dessa desigualdade. Sugerimos, assim, que a noção de justiça, reposicionada pelo reconhecimento, e a discussão de direitos humanos podem ser um caminho, não apenas para lutar contra esta naturalização, mas também para irmos além da cooptação.

Is 'tagging' a rationale for affirmative action in education?

In this paper, we try to rationalize the existence of one of the most common affirmative action policies: educational quotas. We model a two period economy with asymmetric information and endogenous human capital formation. Individuals may be from two different groups in the population, where each group is defined by an observable and exogenous characteristic. The distribution of skills differ across groups. We introduce educational quotas into the model by letting the planner reduce the effort cost that a student from one of the groups has to endure in order to be accepted into a university. Affirmative action policies can be interpreted as a form of ``tagging" since group characteristics are used as proxies for productivity. We find that although educational quotas are usually efficient, they need not subsidize the education of the low skill group.

Affirmative action in college admission and the proficiency of high school students

Economic theory suggests that a¢ rmative action can either reduce or enhance incentives to invest in human capital. Empirical evidence on this matter, however, is still lacking. Using di¤erence in di¤er- ence estimates, this paper evaluates the e¤ects of the quota system in the admission to Brazilian public universities on the pro ciency of high school students. Our ndings show that favored groups attained lower scores, suggesting a negative link between a¢ rmative action and incentives for e¤ort and skill acquisition.