Otto Barblan, Brustbild - Hymne aus der Calvenfeier, Liedanfang, Noten und Text

Signatur des Originals: S 36/F02685

Wilhelm Baumgartner, Brustbild - Baumgartner, Mein Heimatland, Liedanfang in Text und Noten

Signatur des Originals: S 36/F02829

Johannes Brahms, Brustbild - Ständchen, Liedanfang in Noten und Text

Signatur des Originals: S 36/F03148

Thomas Koschat, Brustbild - Liedanfang in Text und Noten: Verlassen verlassen; verlassen bin i!

Signatur des Originals: S 36/F05009

Fragm. lat. VII 61 - Kanonistischer Text

Trägerband: Inc. qu. 873; Vorbesitzer: Dominikanerkloster Frankfurt am Main

Samuel Hassel als Aschemann, Rollenbild, in: Ferdinand Raimund: Der Bauer als Millionär, nebst Liedauszug in Text und Noten

Signatur des Originals: S 36/G03140

Costume Bild zur Theaterzeitung : Die Spinn Scene aus der Oper "Martha", Text von Friedrich, Musik von Flotow

Signatur des Originals: S 36/G14524

Der Tosephtatraktat Roš Haššana : in vokalisiertem Text mit sprachlichen, textkritischen und sachlichen Bemerkungen

von Paul Fiebig

Sayings of the Jewish Fathers : comprising Pirqe Aboth and Pereq R. Meir ; ... with ... specimen pages of the Cambridge University manuscript of the Mishnah 'Jerushalmith' from which the text of Aboth is taken

by Charles Taylor

Deutsches Kolonialrecht : eine orientierende Schilderung der außereuropäischen Erwerbungen des Deutschen Reiches und Darstellung ihrer Rechtsordnung ; nebst dem Text und Erläuterungen der diese Schutzgebiete betreffenden Gesetze und kaiserlichen Verordnungen

von Karl Gareis

Fragm. lat. VIII 84 - Grammatischer Text

Trägerband: 15/481; Vorbesitzer: Dominikanerkloster Frankfurt am Main

Kritischer Commentar zu den Psalmen : nebst Text und Uebersetzung

von H. Graetz

Biographische Skizzen berühmter Feldherren des k. k. österreichischen Heeres von den ältesten bis auf die neuesten Zeiten : in kurzem Auszuge nach Nekrologen der österreichischen Militär-Zeitschrift, und des neuen Archivs für Geschichte, Staatenkunde, Literatur und Kunst ... und mehreren andern gedruckten und ungedruckten Quellen ; als Text zu den in 18 Heften enthaltenen 72 Abbildungen der k. k. österreichischen Generalität

hrsg. von Johann Ritter von Rittersberg

Fluorophotometry is a sensitive method/technique to evaluate epithelial permeability in murine experimental dry eye

Purpose. Fluorophotometry is a well validated method for assessing corneal permeability in human subjects. However, with the growing importance of basic science animal research in ophthalmology, fluorophotometry’s use in animals must be further evaluated. The purpose of this study was to evaluate corneal epithelial permeability following desiccating stress using the modified Fluorotron Master™. ^ Methods. Corneal permeability was evaluated prior to and after subjecting 6-8 week old C57BL/6 mice to experimental dry eye (EDE) for 2 and 5 days (n=9/time point). Untreated mice served as controls. Ten microliters of 0.001% sodium fluorescein (NaF) were instilled topically into each mouse’s left eye to create an eye bath, and left to permeate for 3 minutes. The eye bath was followed by a generous wash with Buffered Saline Solution (BSS) and alignment with the Fluorotron Master™. Seven corneal scans using the Fluorotron Master were performed during 15 minutes (1 st post-wash scans), followed by a second wash using BSS and another set of five corneal scans (2nd post-wash scans) during the next 15 minutes. Corneal permeability was calculated using data calculated with the FM™ Mouse software. ^ Results. When comparing the difference between the Post wash #1 scans within the group and the Post wash #2 scans within the group using a repeated measurement design, there was a statistical difference in the corneal fluorescein permeability of the Post-wash #1 scans after 5 days (1160.21±108.26 vs. 1000.47±75.56 ng/mL, P<0.016 for UT-5 day comparison 8 [0.008]), but not after only 2 days of EDE compared to Untreated mice (1115.64±118.94 vs. 1000.47±75.56 ng/mL, P>0.016 for UT-2 day comparison [0.050]). There was no statistical difference between the 2 day and 5 day Post wash #1 scans (P=.299). The Post-wash #2 scans demonstrated that EDE caused a significant NaF retention at both 2 and 5 days of EDE compared to baseline, untreated controls (1017.92±116.25, 1015.40±120.68 vs. 528.22±127.85 ng/mL, P<0.05 [0.0001 for both]). There was no statistical difference between the 2 day and 5 day Post wash #2 scans (P=.503). The comparison between the Untreated post wash #1 with untreated post wash #2 scans using a Paired T-test showed a significant difference between the two sets of scans (P=0.000). There is also a significant difference between the 2 day comparison and the 5 day comparison (P values = 0.010 and 0.002, respectively). ^ Conclusion. Desiccating stress increases permeability of the corneal epithelium to NaF, and increases NaF retention in the corneal stroma. The Fluorotron Master is a useful and sensitive tool to evaluate corneal permeability in murine dry eye, and will be a useful tool to evaluate the effectiveness of dry eye treatments in animal-model drug trials.^

IDENTIFICATION AND ANALYSIS OF A NOVEL ROLE FOR THE TOUSLED-LIKE KINASE IN REGULATING MITOTIC SPINDLE DYNAMICS

Deregulation of kinase activity is one example of how cells become cancerous by evading evolutionary constraints. The Tousled kinase (Tsl) was initially identified in Arabidopsis thaliana as a developmentally important kinase. There are two mammalian orthologues of Tsl and one orthologue in C. elegans, TLK-1, which is essential for embryonic viability and germ cell development. Depletion of TLK-1 leads to embryonic arrest large, distended nuclei, and ultimately embryonic lethality. Prior to terminal arrest, TLK-1-depleted embryos undergo aberrant mitoses characterized by poor metaphase chromosome alignment, delayed mitotic progression, lagging chromosomes, and supernumerary centrosomes. I discovered an unanticipated requirement for TLK-1 in mitotic spindle assembly and positioning. Normally, in the newly-fertilized zygote (P0) the maternal pronucleus migrates toward the paternal pronucleus at the posterior end of the embryo. After pronuclear meeting, the pronuclear-centrosome complex rotates 90° during centration to align on the anteroposterior axis followed by nuclear envelope breakdown (NEBD). However, in TLK-1-depleted P0 embryos, the centrosome-pronuclear complex rotation is significantly delayed with respect to NEBD and chromosome congression, Additionally, centrosome positions over time in tlk-1(RNAi) early embryos revealed a defect in posterior centrosome positioning during spindle-pronuclear centration, and 4D analysis of centrosome positions and movement in newly fertilized embryos showed aberrant centrosome dynamics in TLK-1-depleted embryos. Several mechanisms contribute to spindle rotation, one of which is the anchoring of astral microtubules to the cell cortex. Attachment of these microtubules to the cortices is thought to confer the necessary stability and forces in order to rotate the centrosome-pronuclear complex in a timely fashion. Analysis of a microtubule end-binding protein revealed that TLK-1-depleted embryos exhibit a more stochastic distribution of microtubule growth toward the cell cortices, and the types of microtubule attachments appear to differ from wild-type embryos. Additionally, fewer astral microtubules are in the vicinity of the cell cortex, thus suggesting that the delayed spindle rotation could be in part due to a lack of appropriate microtubule attachments to the cell cortex. Together with recently published biochemical data revealing the Tousled-like kinases associate with components of the dynein microtubule motor complex in humans, these data suggest that Tousled-like kinases play an important role in mitotic spindle assembly and positioning.