Rapid, reliable and inexpensive quality assessment of biotinylated cRNA

The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (6 were chosen and classified as degraded cRNAs, and 31 samples with a ß-actin 3'/5' ratio <6 were selected as good quality cRNAs. Blinded samples were then used for the RT-PCR assay. After gel electrophoresis, optical densities of the amplified 3' and 5' fragments of ß-actin were measured and the 3'/5' ratio was calculated. There was a strong correlation (r² = 0.6802) between the array and the RT-PCR ß-actin 3'/5' ratios. Moreover, the RT-PCR 3'/5' ratio was significantly different (P < 0.0001) between undegraded (mean ± SD, 0.34 ± 0.09) and degraded (1.71 ± 0.83) samples. None of the other parameters analyzed, such as i) the starting amount of RNA, ii) RNA quality assessed using the Bioanalyzer Chip technology, or iii) the concentration and OD260/OD280 ratio of the purified biotinylated cRNA, correlated with cRNA quality.

Arsenic trioxide reduces the invasive and metastatic properties of nasopharyngeal carcinoma cells in vitro

Nasopharyngeal carcinoma (NPC) is notorious for the metastases, which are in close association with Epstein-Barr virus-encoded latent membrane protein 1 (LMP1). Arsenic trioxide (As2O3) has been shown to induce apoptosis and differentiation in NPC xenografts. Then, can it repress the cancer cells' metastasis potential? To elucidate this issue, the present study was performed. LMP1-negative cell line HNE1 and LMP1-positive cell line HNE1-LMP1 were used as in vitro model. Cells (1 x 10(5)/mL) were cultured with or without 3 µM As2O3 for 48 h. Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. Both confocal immunofluorescence staining and Western blot were used to detect the changes of LMP1 expression. The changes of MMP-9 were examined by RT-PCR assay and Western blot. The results were as follow: i) the colony formation inhibition rate (75.41 ± 3.9% in HNE1-LMP1 cells vs 37.89 ± 4.9% in HNE1 cells), the rate of attachment (HNE1-LMP1 vs HNE1: 56.40 ± 3.5 vs 65.87 ± 5.9%), the invasion inhibitory rate (HNE1-LMP1 vs HNE1: 56.50 ± 3.7 and 27.91 ± 2.1%), and the migration inhibitory rate (HNE1-LMP1 vs HNE1: 48.70 ± 3.9 vs 29.19 ± 6.27%) were all significantly different between the two cell lines (P < 0.01). ii) LMP1 was down-regulated in As2O3-treated HNE1-LMP1 cells. iii) The reduction of MMP-9 was found in As2O3-treated groups, more evident in HNE1-LMP1 cells. Thus, we conclude that As2O3 can reduce metastasis potential of NPC cells, involving inhibition of MMP-9 expression. LMP1 were also reduced in this process and seemed to enhance anti-metastasis activity of As2O3.

Anti-tumor necrosis factor-a for the treatment of steroid-refractory acute graft-versus-host disease

Allogeneic stem cell transplantation has been increasingly performed for a variety of hematologic diseases. Clinically significant acute graft-versus-host disease (GVHD) occurs in 9 to 50% of patients who receive allogeneic grafts, resulting in high morbidity and mortality. There is no standard therapy for patients with acute GVHD who do not respond to steroids. Studies have shown a possible benefit of anti-TNF-a (infliximab)for the treatment of acute GVHD. We report here on the outcomes of 10 recipients of related or unrelated stem cell transplants who received 10 mg/kg infliximab, iv, once weekly for a median of 3.5 doses (range: 1-6) for the treatment of severe acute GVHD and who were not responsive to standard therapy. All patients had acute GVHD grades II to IV (II = 2, III = 3, IV = 5). Overall, 9 patients responded and 1 patient had progressive disease. Among the responders, 3 had complete responses and 6 partial responses. All patients with cutaneous or gastrointestinal involvement responded, while only 2 of 6 patients with liver disease showed any response. None of the 10 patients had any kind of immediate toxicity. Four patients died, all of them with sepsis. Six patients are still alive after a median follow-up time of 544 days (92-600) after transplantation. Considering the severity of the cases and the bad prognosis associated with advanced acute GVHD, we find our results encouraging. Anti-TNF-a seems to be a useful agent for the treatment of acute GVHD.

Leptin levels in different forms of Chagas' disease

Leptin is produced primarily by adipocytes. Although originally associated with the central regulation of satiety and energy metabolism, increasing evidence indicates that leptin may be an important mediator in cardiovascular pathophysiology. The aim of the present study was to investigate plasma leptin levels in patient with Chagas' heart disease and their relation to different forms of the disease. We studied 52 chagasic patients and 30 controls matched for age and body mass index. All subjects underwent anthropometric, leptin and N-terminal pro-brain natriuretic peptide (NT-proBNP) measurements and were evaluated by echocardiography, 12-lead electrocardiogram (ECG), and chest X-ray. All patients had fasting blood samples taken between 8:00 and 9:00 am. Chagasic patients were divided into 3 groups: group I (indeterminate form, IF group) consisted of 24 subjects with 2 positive serologic reactions for Chagas' disease and no cardiac involvement as evaluated by chest X-rays, ECG and two-dimensional echocardiography; group II (showing ECG abnormalities and normal left ventricular systolic function, ECG group) consisted of 14 patients; group III consisted of 14 patients with congestive heart failure (CHF group) and left ventricular dysfunction. Serum leptin levels were significantly lower (P < 0.001) in the CHF group (1.4 ± 0.8 ng/mL) when compared to the IF group (5.3 ± 5.3 ng/mL), ECG group (9.7 ± 10.7 ng/mL), and control group (8.1 ± 7.8 ng/mL). NT-proBNP levels were significantly higher (P < 0.001) in the CHF group (831.8 ± 800.1 pg/mL) when compared to the IF group (53.2 ± 33.3 pg/mL), ECG group (83.3 ± 57.4 pg/mL), and control group (32 ± 22.7 pg/mL). Patients with Chagas' disease and an advanced stage of CHF have high levels of NT-ProBNP andlow plasma levels of leptin. One or more leptin-suppressing mechanisms may operate in chagasic patients.

Possible in vivo mechanisms involved in photodynamic therapy using tetrapyrrolic macrocycles

Photodynamic therapy (PDT) mediated by oxidative stress causes direct tumor cell damage as well as microvascular injury. To improve this treatment new photosensitizers are being synthesized and tested. We evaluated the effects of PDT with 5,10,15,20-tetrakis(4-methoxyphenyl)-porphyrin (TMPP) and its zinc complex (ZnTMPP) on tumor levels of malondialdehyde (MDA), reduced glutathione (GSH) and cytokines, and on the activity of caspase-3 and metalloproteases (MMP-2 and -9) and attempted to correlate them with the histological alterations of tumors in 3-month-old male Wistar rats, 180 ± 20 g, bearing Walker 256 carcinosarcoma. Rats were randomly divided into five groups: group 1, ZnTMPP+irradiation (IR) 10 mg/kg body weight; group 2, TMPP+IR 10 mg/kg body weight; group 3, 5-aminolevulinic acid (5-ALA+IR) 250 mg/kg body weight; group 4, control, no treatment; group 5, only IR. The tumors were irradiated for 15 min with red light (100 J/cm², 10 kHz, 685 nm) 24 h after drug administration. Tumor tissue levels of MDA (1.1 ± 0.7 in ZnTMPP vs 0.1 ± 0.04 nmol/mg protein in control) and TNF-α (43.5 ± 31.2 in ZnTMPP vs 17.3 ± 1.2 pg/mg protein in control) were significantly higher in treated tumors than in controls. Higher caspase-3 activity (1.9 ± 0.9 in TMPP vs 1.1 ± 0.6 OD/mg protein in control) as well as the activation of MMP-2 (P < 0.05) were also observed in tumors. These parameters were correlated (Spearman correlation, P < 0.05) with the histological alterations. These results suggest that PDT activates the innate immune system and that the effects of PDT with TMPP and ZnTMPP are mediated by reactive oxygen species, which induce cell membrane damage and apoptosis.

Gadolinium increases the vascular reactivity of rat aortic rings

Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 µM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 µM L-NAME, 10 µM losartan, or 10 µM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade of E-NTDase activity by Gd, we added 1 mM ATP to the bath. ATP reduced smooth muscle tension and Gd increased its relaxing effect (control: -33.5 ± 4.1; Gd: -47.4 ± 4.1%). Endothelial damage abolished the effect of Gd on the contractile responses to PHE (control: 132.6 ± 8.6; Gd: 122.4 ± 7.1%). L-NAME + Gd in the presence of endothelium reduced PHE contractile responses (control/L-NAME: 151.1 ± 28.8; L-NAME + Gd: 67.9 ± 19% AUC). ATP hydrolysis was reduced after Gd administration, which led to ATP accumulation in the nutrient solution and reduced ADP concentration, while adenosine levels remained the same. Incubation with Gd plus losartan and enalaprilat eliminated the pressor effects of Gd. Gd increased vascular reactivity to PHE regardless of the reduction of E-NTPDase activity and adenosine production. Moreover, the increased reactivity to PHE promoted by Gd was endothelium-dependent, reducing NO bioavailability and involving an increased stimulation of angiotensin-converting enzyme and angiotensin II AT1 receptors.

Short-term follow-up of exercise training program and beta-blocker treatment on quality of life in dogs with naturally acquired chronic mitral valve disease

This study aimed to evaluate the effects of carvedilol treatment and a regimen of supervised aerobic exercise training on quality of life and other clinical, echocardiographic, and biochemical variables in a group of client-owned dogs with chronic mitral valve disease (CMVD). Ten healthy dogs (control) and 36 CMVD dogs were studied, with the latter group divided into 3 subgroups. In addition to conventional treatment (benazepril, 0.3-0.5 mg/kg once a day, and digoxin, 0.0055 mg/kg twice daily), 13 dogs received exercise training (subgroup I; 10.3±2.1 years), 10 dogs received carvedilol (0.3 mg/kg twice daily) and exercise training (subgroup II; 10.8±1.7 years), and 13 dogs received only carvedilol (subgroup III; 10.9±2.1 years). All drugs were administered orally. Clinical, laboratory, and Doppler echocardiographic variables were evaluated at baseline and after 3 and 6 months. Exercise training was conducted from months 3-6. The mean speed rate during training increased for both subgroups I and II (ANOVA, P>0.001), indicating improvement in physical conditioning at the end of the exercise period. Quality of life and functional class was improved for all subgroups at the end of the study. The N-terminal pro-brain natriuretic peptide (NT-proBNP) level increased in subgroup I from baseline to 3 months, but remained stable after training introduction (from 3 to 6 months). For subgroups II and III, NT-proBNP levels remained stable during the entire study. No difference was observed for the other variables between the three evaluation periods. The combination of carvedilol or exercise training with conventional treatment in CMVD dogs led to improvements in quality of life and functional class. Therefore, light walking in CMVD dogs must be encouraged.

Safety and outcome of coronary interventions with special reference to anticoagulation and stent type

University of Turku, Faculty of Medicine, Department of Cardiology and Cardiovascular Medicine, Doctoral Programme of Clinical Investigation, Heart Center, Turku University Hospital, Turku, Finland Division of Internal Medicine, Department of Cardiology, Seinäjoki Central Hospital, Seinäjoki, Finland Heart Center, Satakunta Central Hospital, Pori, Finland Annales Universitatis Turkuensis Painosalama Oy, Turku, Finland 2015 Antithrombotic therapy during and after coronary procedures always entails the challenging establishment of a balance between bleeding and thrombotic complications. It has been generally recommended to patients on long-term warfarin therapy to discontinue warfarin a few days prior to elective coronary angiography or intervention to prevent bleeding complications. Bridging therapy with heparin is recommended for patients at an increased risk of thromboembolism who require the interruption of anticoagulation for elective surgery or an invasive procedure. In study I, consecutive patients on warfarin therapy referred for diagnostic coronary angiography were compared to control patients with a similar disease presentation without warfarin. The strategy of performing coronary angiography during uninterrupted therapeutic warfarin anticoagulation appeared to be a relatively safe alternative to bridging therapy, if the international normalized ratio level was not on a supratherapeutic level. In-stent restenosis remains an important reason for failure of long-term success after a percutaneous coronary intervention (PCI). Drug-eluting stents (DES) reduce the problem of restenosis inherent to bare metal stents (BMS). However, a longer delay in arterial healing may extend the risk of stent thrombosis (ST) far beyond 30 days after the DES implantation. Early discontinuation of antiplatelet therapy has been the most important predisposing factor for ST. In study II, patients on long-term oral anticoagulant (OAC) underwent DES or BMS stenting with a median of 3.5 years’follow-up. The selective use of DESs with a short triple therapy seemed to be safe in OAC patients, since late STs were rare even without long clopidogrel treatment. Major bleeding and cardiac events were common in this patient group irrespective of stent type. In order to help to predict the bleeding risk in patients on OAC, several different bleeding risk scorings have been developed. Risk scoring systems have also been used also in the setting of patients undergoing a PCI. In study III, the predictive value of an outpatient bleeding risk index (OBRI) to identify patients at high risk of bleeding was analysed. The bleeding risk seemed not to modify periprocedural or long-term treatment choices in patients on OAC after a percutaneous coronary intervention. Patients with a high OBRI often had major bleeding episodes, and the OBRI may be suitable for risk evaluation in this patient group. Optical coherence tomography (OCT) is a novel technology for imaging intravascular coronary arteries. OCT is a light-based imaging modality that enables a 12–18 µm tissue axial resolution to visualize plaques in the vessel, possible dissections and thrombi as well as, stent strut appositions and coverage, and to measure the vessel lumen and lesions. In study IV, 30 days after titanium-nitride-oxide (TITANOX)-coated stent implantation, the binary stent strut coverage was satisfactory and the prevalence of malapposed struts was low as evaluated by OCT. Long-term clinical events in patients treated with (TITANOX)-coated bio-active stents (BAS) and paclitaxel-eluting stents (PES) in routine clinical practice were examined in study V. At the 3-year follow-up, BAS resulted in better long-term outcome when compared with PES with an infrequent need for target vessel revascularization. Keywords: anticoagulation, restenosis, thrombosis, bleeding, optical coherence tomography, titanium

Desenvolvimento de massa alimentícia fresca funcional com a adição de isolado protéico de soja e polidextrose utilizando páprica como corante

A tendência do consumidor atual é utilizar alimentos de fácil preparo que, adicionalmente à qualidade nutritiva, tragam benefícios à saúde. Neste trabalho, uma massa alimentícia fresca funcional foi desenvolvida pela incorporação de Isolado Protéico de Soja (IPS) e polidextrose, utilizando páprica como corante. O consumo de IPS pode contribuir para a diminuição dos níveis de colesterol, enquanto a polidextrose, funcionando como fibra, pode promover a redução de risco de câncer de cólon. A otimização da formulação foi realizada utilizando-se um delineamento fatorial completo 23, considerando como variáveis independentes os teores de: i) IPS; ii) polidextrose; e iii) páprica. Os efeitos destas variáveis na qualidade da massa alimentícia foram avaliados em função das características físico-químicas (cor e teste de cozimento), textura instrumental e propriedades sensoriais. Na avaliação dos parâmetros físicos, observou-se que a cor instrumental da massa crua foi influenciada apenas pelo teor de páprica; já o tempo de cozimento (2,5 minutos), o aumento de massa (1,40 a 1,64%) e a perda de sólidos (3,80 a 5,56%) não foram influenciados pelos ingredientes utilizados; e a elasticidade (parâmetro da textura instrumental) foi reduzida com o aumento do teor de polidextrose. Na avaliação sensorial da massa crua, o aumento na porcentagem de IPS e a redução na porcentagem de polidextrose afetaram positivamente a aceitação global e a intenção de compra; e os aumentos nas porcentagens de IPS e de páprica aumentaram a aceitação da cor. Na avaliação sensorial da massa cozida, observou-se que o IPS teve influência positiva sobre a textura, enquanto que a polidextrose e a páprica afetaram negativamente este parâmetro. Entretanto, na avaliação da intenção de compra da massa cozida, não houve influência dos ingredientes adicionados. Os percentuais ideais de adição de polidextrose, isolado protéico de soja e páprica foram de 3,5, 8,0 e 1,5%, respectivamente.

Alteração do teor de cálcio no banho de DP para 2,5 mEq/L é eficaz no reestabelecimento dos valores preconizados por diretrizes atuais em pacientes com PTH < 150 pg/dL

INTRODUÇÃO/OBJETIVO: A doença óssea adinâmica (DOA) é um achado comum em diálise peritoneal (PD) e é considerada fator de risco para desenvolvimento de fraturas e doença cardiovascular. Dados do BRAZPD apontam as soluções de cálcio a 3,5 mEq/L presentes na maioria das prescrições no país, que possui quase 9.000 pacientes em PD. É comum o balanço positivo de cálcio com concentrações a 3,5 mEq/L contribuindo para o desenvolvimento de DOA. Diretrizes atuais recomendam um PTHi na DRC V em diálise entre 2 e 9 vezes (150-500 pg/mL) o valor máximo da normalidade. O objetivo deste estudo foi avaliar a resposta em 6 meses do PTH-i após a conversão para solução de cálcio a 2,5 mEq/L de pacientes que usavam soluções com cálcio a 3,5 mEq/L e com PTH-i basal < 150 pg/mL. MÉTODOS: Coorte prospectiva, observacional, na qual todos pacientes prevalentes em PD entre janeiro de 2008 e janeiro de 2009 de um único centro foram incluídos. Critérios de inclusão foram:(1) estar em uso de solução de DP com cálcio a 3,5 mEq/L; (2)PTH sérico < 150 pg/mL. A critério médico, os pacientes poderiam ser convertidos ou não para soluções cálcio a 2,5 mEq/L. RESULTADOS: 35 pacientes (idade média 62 ± 17 anos) foram incluídos. Desses, 22 foram convertidos para solução de cálcio 2,5 mEq/L. Nefropatia diabética foi a principal doença de base (36%) seguido por HAS (25%) e GNC (14%). Os grupos apresentavam valores basais semelhantes de PTH, cálcio, fósforo e fosfatase alcalina. No grupo-intervenção, houve aumento significativamente maior de PTH em 6 meses comparado com o grupo-controle (Δ194 pg/dL versus Δ 92/dL; p < 0,05). Dos convertidos, 41% atingiram os valores alvo de PTH contra 14% (p < 0,05) do grupo-controle. Fósforo, cálcio e fosfatase alcalina foram semelhantes entre os grupos. CONCLUSÃO: O uso de soluções com cálcio a 2,5 mEq/L para pacientes com PTHi < 150 pg/dL comparado a soluções de cálcio 3,5 mEq/L parece uma estratégia simples e efetiva para trazer os valores de PTHi dentro da faixa atualmente sugerida nas diretrizes.

Absorção de água por semente de salsa, em duas temperaturas

No presente trabalho, objetivou-se estabelecer a duração da fase II da germinação de semente de salsa de diferentes cultivares e procedências, em duas temperaturas de embebição, para posterior planejamento do condicionamento osmótico. Semente de salsa, cultivares Lisa e Portuguesa, lotes 1 e 2, foi colocada para embeber em caixa gerbox com papel filtro previamente umedecido, nas temperaturas de 25º e 30º C, em germinador do tipo Mangelsdorf. Para cada tratamento, foram utilizadas quatro repetições de 1,0g de semente. As avaliações foram realizadas a cada 24 horas, quando a semente era retirada do gerbox, secada criteriosamente e pesada em balança digital (0,001g), até a protrusão da raiz primária de pelo menos uma semente de cada repetição dos tratamentos, quando a semente era descartada e considerada a leitura anterior. Estabeleceu-se, para cada tratamento, uma equação (3º grau) que se ajustasse ao padrão trifásico da germinação e delimitasse o início, o final e a duração da fase II do processo germinativo. Após a derivação da equação de 3º grau, determinaram-se as raízes da equação derivada (2º grau), os pontos de inflexão das curvas e a quantidade de água absorvida pela semente, até o início da fase II (primeiro ponto de inflexão da curva). A fase II da embebição de semente de salsa iniciou-se entre 59,7 e 93,3 horas. A duração da fase II da embebição foi entre 56,7 e 91,6 horas. Em semente de salsa, a interrupção do condicionamento osmótico, na temperatura de 25º C, deve ser entre 122,5 e 148,4 horas e na temperatura de 30º C, entre 124,9 e 184,9 horas.

[5 lettres de Silvain à Jules Massenet, 1886-[1912]]

f.1, 2-3, 5-6 : lettres, f.5 : carte

全人矩矱Quan ren ju he.La règle de tout l'homme.

Contient : I, livre préliminaire太上感應篇纂註Tai shang gan ying pian zuan zhu.Le Tai shang gan ying pian, avec commentaires ; II, livre préliminaire文昌帝君陰隲文纂註Wen chang di jun yin zhi wen zuan zhu.Le Yin zhi wen, du dieu de la Littérature, avec commentaires ; III, livre préliminaire文昌帝君覺世文纂註Wen chang di jun jue shi wen zuan zhu.Traité pour éveiller le monde, par le dieu de la Littérature ; IV, livre préliminaire關聖帝君覺世經 。釋略并諭Guan sheng di jun jue shi jing. Shi lüe bing yu.Le vrai livre sacré du réveil du monde, par le dieu de la Guerre ; V, livre préliminaire純陽祖師警士文Chun yang zu shi jing shi wen.Avertissement aux lettrés, traité du dieu Fu you ; VI, livre préliminaire魏元君勸世文Wei yuan jun quan shi wen ; VII, livre 1勸孝集說Quan xiao ji shuo.Recueil de textes recommandant la piété filiale ; VIII, livre 2戒淫集說Jie yin ji shuo.Recueil de textes contre l'impureté ; IX, livre 2文昌帝君戒淫文Wen chang di jun jie yin wen.Traité contre l'impureté, par le dieu de la Littérature ; X, livre 2文昌帝君天戒錄Wen chang di jun tian jie lu.Défenses célestes, notées par le dieu de la Littérature ; XI, livre 2三丰張眞人戒淫說San feng zhang zhen ren jie yin shuo.Traité contre l'impureté, par l'homme vrai Zhang, de San feng ; XII Livre 2文昌帝君慾海廻狂寶訓Wen chang di jun yu hai hui kuang bao xun.Instructions pour tirer l'insensé de la mer de la concupiscence, par le dieu de la Littérature ; XIII, livre 3勸戒彚抄Quan jie hui chao.Recueil de conseils et de défenses.Wen di quan zhong.Traité pour conseiller la fidélité, par le dieu de la Littérature ; XIV, livre 3文帝戒士子文Wen di jie shi zi wen.Conseils aux lettrés, par le dieu de la Littérature ; XV, livre 3文帝鱣壇勸世文Wen di zhan tan quan shi wen.Conseils donnés au monde par le dieu de la Littérature, à l'autel Zhan ; XVI, livre 3文帝鱣壇語錄Wen di zhan tan yu lu.Entretiens du dieu de la Littérature à l'autel Zhan ; XVII, livre 3蕉窻十則并序Jiao chuang shi ze bing xu.Les dix commandements de Jiao tchhoang, avec préface ; XVIII, livre 3文帝三教論 。七願 。十戒Wen di san jiao lun. Qi yuan. Shi jie.Dissertation sur les trois religions ; les sept vœux ; les dix commandements : du dieu de la Littérature ; XIX, livre 3呂祖師訓世文Lü zu shi xun shi wen.Instructions au monde par le dieu Fu you ; XX, livre 3勸敬惜字紙文Quan jing xi zi zhi wen.Traité pour conseiller le respect des caractères d'écriture ; XXI, livre 3敬字說Jing zi shuo.Traité sur le respect des caractères d'écriture ; XXII, livre 3惜字說Xi zi shuo.Traité sur le ménagement des caractères d'écriture ; XXIII, livre 3勸敬惜五榖文Quan jing xi Wu gu wen.Traité du respect des cinq sortes de grains ; XXIV, livre 3戒殺生說Jie sha sheng shuo.Traité pour défendre de tuer les êtres ayant vie ; XXV, livre 3戒溺女說Jie ni nü shuo.Traité pour défendre de noyer les petites filles ; XXVI, livre 3戒賭文Jie du wen.Traité contre le jeu ; XXVII, livre 3戒嗜酒Jie shi jiu.Défense d'aimer le vin ; XXVIII, livre 3戒貪財Jie tan cai.Défense de convoiter les richesses ; XXIX, livre 3戒聽讒Jie ting chan.Défense d'écouter la calomnie ; XXX, livre 3戒口過文Jie kou ge wen.Traité pour interdire les péchés de langue ; XXXI, livre 3戒損人利己說Jie sun ren li ji shuo.Traité pour interdire de chercher des avantages personnels au détriment d'autrui ; XXXII, livre3不自棄文Bu zi qi wen.Traité recommandant de ne pas s'abandonner au vice ; XXXIII, livre 3戒慢葬Jie man zang.Traité pour défendre d'user de négligence dans les funérailles ; XXXIV, livre 3戒不修治墳墓Jie bu xiu zhi fen mu.Traité recommandant d'entretenir les tombes ; XXXV, livre 3勸塾師培植子弟Quan shu shi pei zhi zi di.Traité recommandant aux maîtres de prendre soin de leurs élèves ; XXXVI, livre 3勸尊重師傅Quan zun zhong shi fu.Traité recommandant aux élèves d'honorer leur maître ; XXXVII, livre 3勸幕賓Quan mo bin.Conseils aux secrétaires privés des mandarins ; XXXVIII, livre 3勸公門修行Quan gong men xiu xing.Dans les affaires publiques, il faut régler sa conduite ; XXXIX, livre 3勸擇術慎業Quan ze shu shen ye.Il faut choisir un métier et s'y appliquer ; XL, livre 3勸化惡助善Quan hua e zu shan.Il faut corriger le mal et contribuer au bien ; XLI, livre 3勸排難解紛Quan phai nan jie fen.Il faut écarter les obstacles et dissiper les difficultés ; XLII, livre 3勸謙和雍睦Quan qian he yong mu.Traité pour conseiller l'esprit de conciliation et de concorde ; XLIII, livre 3勸救濟Quan jiu ji.Il faut aider (ceux qui ont besoin d'aide) ; XLIV, livre 3賑饑十二善Zhen ji shi er shan.Il faut nourrir les affamés ; XLV, livre 3勸敬說Quan jing shuo.Traité sur le respect ; XLVI, livre 3勸畏說Quan wei shuo.Traité sur la crainte respectueuse ; XLVII, livre3改過之法Gai ge zhi fa.Moyen de corriger les fautes ; XLVIII, livre3勸惜福Quan xi fu.Il faut ménager sa part de bonheur ; XLIX, livre3耕心說Geng xin shuo. Autre titre : 勸耕治心地Quan geng zhi xin di.Il faut cultiver son cœur ; L, livre3廣立命說Guang li ming shuo.Développement du traité sur la direction de la vie ; LI, livre3為學當從事性道Wei xue lang cong shi xing dao.Pour étudier, il faut s'appliquer à la voie naturelle ; LII, livre3性道集說Xing dao ji shuo.Recueil de textes sur la voie naturelle ; LIII, livre3夢覺圖說Mong jue tu shuo.Traité et figure sur l'illusion universelle ; LIV, livre4功過格彚編Gong ge ge hui bian.Liste des mérites et des péchés ; LV, livre4行功過格說Xing gong ge ge shuo.Traité sur l'usage de l'échelle des mérites et des péchés ; LVI, livre4功過格例Gong ge ge li.Règles annexes à l'échelle des mérites et des péchés ; LVII, livre4功過格分類彚編Gong ge ge fen lei hui bian.Liste méthodique des mérites et des péchés ; LVIII, livre 4不費錢功德例Bu fei qian gong de li.Manière d'obtenir des mérites sans dépenser d'argent ; LIX, livre final勸世詩歌Quan shi shi ge.Recueil de poésies morales

Recueil de lettres et de pièces originales, et de copies de pièces indiquées comme telles dans le dépouillement qui suit.

Contient : 1 Lettre de « RENEE DE FRANCE [duchesse de Ferrare]... à monseigneur Fumée, conseillier et maistre des requestes du roy... De Ferrare, le VIIe de may 1540 » ; 2 Lettre de GEORGES « DE SELVE, evesque de Lavaur... à madame... la duchesse de Chartres... De Venise, ce XVIIme de febvrier 1533 » ; 3 Lettre de « MARGUERITE [DE VALOIS]... royne de Navarre... à... monseigneur le comte de Vilars » ; 4 Ordonnance d'«HONORAT DE SAVOYE,... pour pourveoir aux abuz qui se commettent journellement sur le port des pacquestz et lettres de particuliers que aulcuns des chevaulcheurs et ceulx qui tiennent postes... reçoipvent et font courir en grand nombre pour leur plaisir... ne concernans aulcunement les affaires et service du roy... Donné à Montpellier, le troysme jour de novembre l'an mil V.C. cinquante » ; 5 Lettre de GEORGES « DE SELVE, evesque de Lavaur... à madame... la duchesse de Ferrare,... De Venise, ce IXme de septembre 1534 » ; 6 Lettre d'« ADRIANE D'ETOUTEVILLE,... à madame... la duchesse de Guise,... De Try, ce VIIe juing 1553 » ; 7 Lettre de « JAQUES [DE SAVOIE]..... à monseigneur Dugué,... Au camp devant Vulpian, ce XVIIIe septembre 1555 » ; 8 Lettre de GEORGES « DE SELVE, evesque de Lavaur... à madame... la duchesse de Ferrare,... De Venise, ce XXVIIme de septembre 1534 » ; 9 Lettre, en italien, d'«OCTAVIANUS DE CAMPOFRANCO » et du Conseil des Anciens de Gênes, en faveur des marchands génois. « Data Janue, die XXVIa maii M.D.XVI » ; 10 Lettre, en italien, de « GALEATIO VESCONTE,... al signore... Robertet,... In Luciera, a XXVIIII novembre 1521 » ; 11 Lettre, en italien, d'« ANTONIUS DE JOFFIS,... ad monsignore il thesorier Robertet,... Rome, die X decembris 1521 » ; 12 Lettres closes « de par le roy... [FRANÇOIS Ier]... à... Pierre Chaboulle, marchant demourant à Meaulx... Fevrier mil V.C. vingt sept ». Copie ; 13 « Lettre du duc de Brunsvich » [HENRI IV, dit le Jeune], signée : « Raince,... al' ill. Sor Filiberto, principe d'Orange,... Presso de Castellione di Cremonese, alli 16 de junio 1528 ». En italien ; 14 Lettre, en italien, de « ALFONSO DE ESTE » au roi François Ier. « Ferrariae, XX maii 1530 » ; 15 Lettre, en italien, de « RANCO » au roi François Ier. « Da Cremona, a li XIX di VIIbre nel M.D.XXX » ; 16 Lettre de « BENEDICTO DE NOBILI,... à madame... de Ferrare,... De Paris, ce dernier de novembre 1512 » ; 17 Lettre de « DODIEU [DE VELY]... à madame [Renée de France, duchesse de Ferrare]... Escript à Boullongne, ce XVIIe jour de janv[i]er 1533 » ; 18 Lettre des « consulz de la vile du Puy... à monseigneur le duc de Nemours,... Du Puy, ce XXVIIIe aoust 1545 » ; 19 Lettre de « M. FUMEE, RENE CHARION, GUEFFIER,... à madame [Renée de France, duchesse de Ferrare]... De Paris, le XIIIe jour d'octobre » ; 20 Lettre, en italien, de « HIERONYMO ROVERO,... Da Pral°, alli XXIIII di febraro M.D.LIII » ; 21 Lettre, en italien, de « ALFONSO DA ESTE,... all' illustrissimo... signor duca di Nemourse,... Di Ferrara, il primo di novembre 1554 » ; 22 Lettre de « NICOLAS DE LORRAINE [comte DE VAUDEMONT]... à... monseigneur le duc de Nemours,... De Nancy, ce IIIme may 1555 » ; 23 Lettre de « M. FUMEE, LE CLERC, GUEFFIER,... à madame [Renée de France], à Ferrare... De Paris, ce XXIIIIe jour de may 1555 » ; 24 « Coppie de l'arrest du compte general de Me Claude Haligre, de l'an V.C.LIII, duquel arrest et closture il est appellant... Faict le vingt ungiesme jour de juillet, l'an mil cinq cens cinquante cinq. Ainsi signez : M. Fumée, Le Clerc, Adam de Houdon, Jehan Baptiste Gondy, Gueffier, Debigny » ; 25 « Lettres du conseil », signées : « GUEFFIER,... à monseigneur... de Varennes, bailly et cappitaine de Gisors... De Paris, ce VIme jour d'aoust 1555 » ; 26 Lettre de « M. FUMEE, LE CLERC, JEHAN BATISTE GONDY, GUEFFIER,... à madame [Renée de France], à Ferrare... De Paris, le VIIe jour d'aoust 1555 » ; 27 « Minute de procuration » de « RENEE DE FRANCE ». 1555 ; 28 Lettre de « JAQUES DE SAVOYE,... De Talloueres, ce XVIme de mars 1561 » ; 29 Lettre de « NICOLAS DE LORRAINE,... à... monsieur... le duc de Nemours,... De Nomeny, ce XXIIme d'apvril 1556 » ; 30 « Dupplicata d'une lettre escripte à madame [Renée de France, duchesse de Ferrare]... De Paris, ce Ve may V.C.LVI, ainsy signé : M. FUMEE, A. FUMEE, N. LE CLERC et GUEFFIER » ; 31 Lettre de « JEHANNE DE SAVOYE,... à... monsieur de Nemours,... 1556 » ; 32 Lettre de « LUIGI DA ESTE,... all' ill... duca d'Anemors,... A XIX di ottobre del LVII. Di Ferrara » ; 33 « Lettres du conseil », signées : « M. FUMEE, PH. MARON, PECQUILLAULT,... à madame [Renée de France]... A Paris, ce XIIIIe fevrier, l'an mil cinq cens trente sept » ; 34 Lettre, en italien, de « ALFONSO DE ESTE,... all' ill... duca di Nemours,... Di Ferrara, il XXIIII de febrajo M.D.LVII » ; 35 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... signora duchessa di Ferrara,... Di Parigi, li XXX di marzo M.D.LVII » ; 36 Lettre de « GIVRY,... à monsigneur... le duc de Nemours,... De Viterbe, ce 18 d'apvril 1557 » ; 37 Lettre du conseil duroi, signée : « M. FUMEE, A. FUMEE, N. LE CLERC, GUEFFIER,... à madame [Renée de France]... De Paris, ce cinqme jour de may V.C.LVI » ; 38 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... signora duchessa di Ferrara,... Di Parigi, a 5 di maggio M.D.LVII » ; 39 « Lettre du conseil », signée : « M. FUMEE, N. LE CLERC, JEHAN BATISTE GONDE, GUEFFIER,... à madame [Renée de France]... De Paris, le XVme jour de may 1557 » ; 40 Lettre, en italien, d'« ALFONSO DE ESTE,... all' ill... duca de Nemours,... Di Ferrara, il XV di maggio del LVII » ; 41 Lettre, en italien, de « LUIGI DA ESTE,... all' ill...duca di Anemors... A XVI di maggio del LVII, di Ferrara » ; 42 Lettre, en italien, de « ALFONSO DE ESTE,... all' ill... duca di Nemours,... Di Modena, il III di giugno del LVII » ; 43 Lettre, en italien, de « GIOVAN BATISTA GONDE,... all' ill... signora duchessa di Ferrara,... Di Parigi, alli XIIII di giugno M.D.LVII » ; 44 Lettre du conseil du roi, signée : « M. FUMEE, N. LE CLERC, JEHAN BATISTE GONDE, GUEFFIER,... à madame [Renée de France]... De Paris, le XVIIIme jour de juing 1557 » ; 45 Lettre, en italien, de « JEHAN BATISTE GONDE » à Renée de France, duchesse de Ferrare. « Di Parigi, alli XX di giugnio M.D.LVII » ; 46 Lettre de « MARC HAULTREUE,... à madame [Renée de France]... De Lucerre, ce XXIIIIe jour de may 1544 » ; 47 Lettre de « JAQUES DE SAVOYE,... De Talloueres, ce XXVme de juing 1556 » ; 48 Lettre de « ADAM DE HOUDON, C. HALIGRE, BARTHELEMY, PECQUILLAULT », à Renée de France. « A Paris, le vingt sixiesme jour de may, l'an mil cinq cens quarente ung » ; 49 « Récépissé de MARC DE HAULTEREUE, maistre d'hostel de monseigneur le cardinal d'Armaignac,... A Breseil, le XXVIe jour de may, l'an mil V.C. quarente quatre » ; 50 Lettre de « GENLY,... à monseigneur... le duc de Nemors,... D'Ascoly, ce XVIe juillet 1557 » ; 51 Lettre de « JAQUES DE SAVOYE,... De Talloueres, ce XVIIme d'aoust 1556 » ; 52 Lettre, en italien, de « li priori del Monte Cassino... all' ill... duca di Nemors,... Dal Monte Cassino, il di XX di luglio del 57 » ; 53 Lettre de « NICOLAS DE LORRAINE,... [comte] DE VAUDEMONT,... à... monseigneur le duc de Nemours,... De Compiegne, le XXIIIIe jullet 1557 » ; 54 « Lettres du conseil », signées : « M. FUMEE, BARTHELEMY, PECQUILLAULT,... à madame [Renée de France]... A Paris, ce vingtcinqme jour de juillet, l'an mil cinq cens quarante et ung » ; 55 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... duchessa di Ferrara,... Di Parigi, alli XXVIII d'agosto 1557 » ; 56 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... duchessa di Ferrara,... Di Parigi, alli XXVIII d'agosto, M.D.LVII » ; 57 Lettre, en italien, du « principe Dyrrachiense, ANDREA ANGELO,... all' ill... duca di Nemur, il Sor Jacobo de Sabaudia,... Da Casa, a gli 3 settembre 1557 » ; 58 Lettre de « JAQUES DE SAVOYE,... De Paris, ce XXIIe septembre 1556 » ; 59 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... duchessa di Ferrara,... Di Parigi, alli XXIIII d'ottobre M.D.LVII » ; 60 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill... duchessa di Ferrara,... Di Parigi, alli IIII di novembre M.D.LVII » ; 61 Lettre de « N. LE CLERC, ADAM DE HOUDON, JEHAN BATISTE GONDE, GUEFFIER,... à madame [Renée de France]... De Paris, le XXVe jour d'octobre V.C.LVI » ; 62 Lettre de « NICOLAS DE LORRAINE,... à... monsieur le duc de Nemours,... De Nommeni, ce XXVe octobre 1556 » ; 63 Lettre du conseil, signée : « M. FUMEE, JEHAN BATISTE GONDE,... à madame [Renée de France]... De Paris, ce XXIIme jour de decembre 1557 » ; 64 Lettre, en italien, de « GIOVAN BATISTE GONDE,... all' ill...[du] chessa di Ferrara,... Di Parigi, a 10 di dicembre M.D.LVII »

Recueil de lettres et de pièces originales, et de copies de pièces indiquées comme telles dans le dépouillement qui suit.

Contient : 1 Lettre du roi « HENRY [II]... à mon conpere » Anne de Montmorency ; 2 Lettre du roi « HENRY [II]... à mon conpere » Anne de Montmorency ; 3 Lettre du roi « H[ENRI II]... à mon conpere » Anne de Montmorency ; 4 Lettre du roi « HENRY [II]... à monsyeur le connetable » Anne de Montmorency ; 5 « Ce qui a esté accordé pour le combat entre Mr de La Chastaigneraye et de Jarnac,... Faict à St Germain en Laye, le sixme jour de juillet M.V.C.LXVII ». Signé : « FRANÇOYS » DE LORRAINE, duc DE GUISE, et « BOYSY » ; 6 Lettre de « CATERINE [DE MEDICIS]... à... monsieur le conestable et deuc de Momoransi » ; 7 Lettre de « HENRY [D'ALBRET]... roy de Navarre... à... monseigneur le grant maistre... A Pau... febvrier » ; 8 Lettre de « MARGUERETE [DE VALOIS, reine de Navarre]... au roy » ; 9 Lettre de « HENRY [D'ALBRET, roi de Navarre]... à... monseigneur le grand maistre » ; 10 Lettre de « MARGUERITE [DE VALOIS, reine de Navarre]... au roy » ; 11 Bref du pape « JULIUS III,... Henrico [II]... Francorum regi... Datum Romae, apud Sanctum Petrum... die prima octobris M.D.LI ». En latin ; 12 Bref du pape « JULIUS... III,... Annae de Montmoransi, regni Franciae comestabili... Datum Romae, apud Sanctum Petrum... die prima octobris M.D.LI ». En latin ; 13 Lettre de « HENRY [D'ALBRET]... roy de Navarre... à... monseigneur le grant maistre » ; 14 Lettre de « MARGUERITE [DE VALOIS, reine de Navarre]... au roy » ; 15 Bref du pape « JULIUS... III,... Anne, domino de Montmoransi,... Datum Romae, apud Sanctum Petrum... die XXVIII octobris M.D.LIIII » ; 16 Lettre de « MARGUERITE [DE VALOIS]... duchesse [d'Alençon]... au roy » ; 17 « Estat des pensions, gaiges et autres entretenemens ordinaires que le roy a ordonné estre paiées sur les deniers de son espargne pour l'année ; 18 « Estat à maistre Nicolas Berthereau, notaire et secretaire du roy, et tresorier et payeur des gaiges des officiers domestiques dudict seigneur, durant l'année