Identificação da Leptospira interrogans sorovar pomona em camundongos geneticamente selecionados, para a alta e baixa produção de anticorpos, através da técnica de imunoperoxidase em tecido renal e isolamento bacteriano em meio de Fletcher

O presente trabalho teve por objetivo identificar a presença da Leptospira interrogans sorovar pomona em camundongos geneticamente selecionados para a alta e baixa resposta a anticorpos. Todos os animais foram submetidos ao isolamento bacteriano, imunohistoquímica (imunoperoxidase) em cortes de tecido renal e coloração através da hematoxilina-eosina. A técnica de imunoperoxidase apresentou-se pouco mais sensível em relação ao cultivo, entretanto, ambas foram bons parâmetros de identificação do agente. Presença de lesões renais mais intensas ocorreram em períodos em que houve maior número de bactérias isoladas em meio de cultivo. Camundongos da linhagem HIV-A conseguiram eliminar as leptospiras com maior eficiência e rapidez em relação as linhagem LIV-A, entretanto o estudo demonstrou que ambas linhagens da seleção IV-A foram eficientes em controlar o processo infeccioso.

Avaliação do Metabolismo Nutricional em Poedeiras pela Técnica dos Isótopos Estáveis do Ccarbono (13C/12C)

Os isótopos estáveis do carbono que eram utilizados em estudos ecológicos e paleoecológicos apresentaram um incremento nos últimos trinta anos, na utilização em estudos dietéticos em animais. Entretanto, existem poucas informações sobre o padrão metabólico e sobre as taxas de turnover do 13C em aves. O presente experimento estabeleceu curvas de substituição e taxas de movimentação do 13C no ovo e no fígado de aves de postura adultas, pela substituição da ração comercial por dietas compostas de grãos dos ciclos fotossintéticos C3 e C4, durante 50 dias. A diferença no conteúdo isotópico do delta per mil do carbono-13 (delta 13C) entre as duas dietas foi de 16,13 . A taxa de substituição do 13C das dietas, nos tecidos, adequou-se num modelo exponencial, descrevendo o turnover do carbono nos tecidos analisados. As taxas de movimentação do 13C, nas aves alimentadas com dieta baseada em grãos C3, foi maior no fígado em relação ao ovo, com valores para a meia-vida de 2,9 e 3,7 dias, respectivamente. As aves que receberam ração com grãos C4 apresentaram uma taxa de turnover no ovo superior àquela obtida para o fígado, com meia-vida de 4,0 e 5,3 dias, respectivamente. Os valores do delta 13C observados para ovo e fígado diferiram em aproximadamente 2 daqueles referentes às dietas.

Avaliação de uma Técnica de Diluição e Transporte de Sêmen Eqüino, para as Condições Brasileiras

Ninety-seven Mangalarga Marchador mares were allocated in a randomized experimental design, with different number of replicates per treatments. The objective of this experiment was to study the effect of raw (T3) or diluted, cooled semen transported to the breeding farms 1 (T1) and 3 (T2), on mare fertility and different aspects of reproductive efficiency. The mares were inseminated, with semen of only one stallion (Farm 2), on alternate days using different inseminating volumes, according to number of inseminated mares per day using only one ejaculate. For transportation, semen diluted in a lactose-egg yolk modified extender and the MSP-2 container were used. There was no difference among treatments in relation to conception rates of mares inseminated with raw or diluted, cooled and transported semen. Regardless of the farm, the total fertility obtained with cooled semen was 80% (52/65). The following variables were not affected by the treatments: cycles/mare, cycles/pregnant mare, cycles/pregnancy, pregnancy/cycle, number of inseminations/mare, number of inseminations/pregnant mare, number of inseminations/open mare and efficiency of pregnancy. However, the breeding farm had an effect on water and semen temperature in the container, time between collection and insemination, according to time and distance covered. Based on the results, cooling and transporting equine semen, using an extender, lactose-egg yolk without glycerine and the MSP-2 container should be recommended for short distances. The use of raw semen immediately after collection is also recommended.

Predição de propagação de fissuras através de modelos constitutivos locais e técnica de construção progressiva da trajetória de descontinuidade

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Planejamento de Fontes Reativas em Sistemas de Energia Elétrica utilizando a técnica de Decomposição de Benders e o algoritmo de Branch-and-Bound

This paper presents the Benders decomposition technique and Branch and Bound algorithm used in the reactive power planning in electric energy systems. The Benders decomposition separates the planning problem into two subproblems: an investment subproblem (master) and the operation subproblem (slave), which are solved alternately. The operation subproblem is solved using a successive linear programming (SLP) algorithm while the investment subproblem, which is an integer linear programming (ILP) problem with discrete variables, is resolved using a Branch and Bound algorithm especially developed to resolve this type of problem.

Técnica alternativa de conformação de junção a partir de tubos metálicos

This work presents a technique for the cold forming of thin-walled cylindrical tubes, aimed at obtaining a symmetrical joint with an unconventional forming technique, using elastomer rod. The purpose of this work is to investigate the influence of the anisotropy on the total forming force, through the use of elastomer for obtaining T-junctions, in order to get a better result between experimental values and the analytical solution. For total forming force forecasting, it will be used the upper-bound theory and for a better understanding on the anisotropy behavior of the material, it will be employed the quadratic yielding criterion proposed by Hill. The employed materials for such investigation were Aluminum, Brass and Copper.

Prototipagem como forma alternativa para realização de cranioplastia com metilmetacrilato: Nota técnica

The prototyping is a method for reconstruction of human body segments by computer software. It has been used in neurosurgery for cranial reproduction in patients allowing the programming of surgical procedures and the production of prosthesis to reconstruct bone failures in the skull. We present two cases of cranioplasty performed with the use of acrylic prosthesis constructed by prototyping. After 10 months of follow-up, they donot present signs of infection and show good aesthetic result. The advantages pointed at the literature for this method (reduction of surgical time, easy technical handle, and good aesthetic result) were confirmed.

Aplicação da técnica de condicionamento total ou de primers autocondicionantes em dentes decíduos após abrasão a ar

Since the use of air abrasion has grown in pediatric dentistry, the aim of this study was to evaluate, by means of shear bond strength testing, the need to use the total etching technique or self-etching primers on dentin of primary teeth after air abrasion. Twenty-five exfoliated primary molars had their occlusal dentin exposed by trimming and polishing. Specimens were treated by: Air abrasion + Scotchbond MultiPurpose adhesive (G1); 37% phosphoric acid + Scotchbond MP adhesive (G2); Clearfil SE (G3); Air abrasion ( 37% phosphoric acid + Scotchbond MP adhesive (G4); Air abrasion + Clearfil SE (G5). On the treated surface, a cylinder of 2 mm by 6 mm was made using a composite resin (Z100). Duncan's test showed that: (G2 = G3 = G5) > (G1 = G4). The use of a selfetching primer on air abraded dentin is recommended to obtain higher bond strengths.

Técnica autorradiográfica para investigação geológica - Estudo de caso na área do projeto rio preto

The auto-radiography is a photographic method to registrate in sensitive emulsion the spatial distribution a rays emitted by radioisotopes of a sample or an object. The auto-radiography was applied to detect the presence of radioactive minerals in some samples of schists and gneisses from the Ticunzal Formation, Northeast Goiás State, aiming to implement the use of this technique in LABIDRO - Hydrochemistry and Isotopes Laboratory of the Department of Petrology and Metallogenesis, State University of São Paulo/Campus of Rio Claro.

Avaliação da distribuição da densidade em MDF a partir da técnica da onda de ultra-som

The goal of this research was to determine the density distribution in medium density fiberboard (MDF), manufactured with polyurethane derived from castor oil using, ultrasonic wave technique. The equipment used in this test is Steinkamp BP7 with plan and exponential transducers, both with 45 kHz frequencies, located in several zones on the plate in order to determine wave ultrasonic velocity. The Pinus caribaea and Eucalyptus grandis fiberboard were manufactured in the quality control and products development laboratory of Duratex with 500 mm long, 500 mm large, 8 and 15 mm of thickness. Three MDF for each fiber specimen and thickness were fabricated, totalizing twelve plates tested. The MDF were produced with 5% polyurethane addition, in temperature of 160°C, tension press of 53 bars and addition of moisture content of 12%. For determination of fiberboard density, samples were extracted from the same zones where the wave ultrasonic velocity was determined. In this case, DAX-Ray equipment was used. Statistical analysis shows good agreement with wave ultrasonic velocity and the density profile, validating the application of non-destructive technique in order to determine the density profile of MDF's.

Disfunção temporária do nervo lingual após uso de máscara laríngea. Relato de caso

BACKGROUND AND OBJECTIVES: The laryngeal mask has been frequently used in Anesthesiology. Although the rate of complications with this technique is smaller than that of the endotracheal tube, it is not devoid of risks, especially in cases of difficult airways. The objective of this study was to report a case of unilateral lingual nerve damage after the use of the laryngeal mask airway. CASE REPORT: A female patient underwent a surgical procedure for removal of bilateral breast prosthesis under general, balanced anesthesia, with a size three laryngeal mask. The balloon was inflated with 30 mL of air. After the first postoperative hour, she developed decreased sensation and pain in the oropharynx and posterior two thirds of the tongue, which evolved for loss of taste in the next 24 hours. A tentative diagnosis of lingual nerve neuropraxis secondary to the use of the laryngeal mask was made. After three weeks, her symptoms subsided. CONCLUSION: Although complications after the use of the laryngeal mask airway are rare, they do occur, and neuropraxis of the lingual nerve is one of them. The diagnosis is clinical and it has a good outcome, with resolution of the symptoms within a few weeks or months. © Sociedade Brasileira de Anestesiologia, 2007.