1000 resultados para Soil microbiology.
Resumo:
The objective of this work was to evaluate the effect of moisture and temperature on the development of Sclerotium rolfsii on soybean, corn, and wheat straw. Wheat straw produced the lowest number of sclerotia. Intermediate soil moisture level (70% of field capacity), and temperatures ranging between 25-30ºC favored sclerotia development. No sclerotia were formed at temperatures between 30-35ºC, on any type of straw.
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The objective of this work was to evaluate the change in soil C and N mineralization due to successive pig slurry application under conventional tillage (CT) and no tillage (NT) systems. The experiment was carried out in a clayey Latossolo Vermelho eutrófico (Rhodic Eutrudox) in Palotina, PR, Brazil. Increasing doses of pig slurry (0, 30, 60 and 120 m³ ha-1 per year) were applied in both tillage systems, with three replicates. Half of the pig slurry was applied before summer soil preparation, and the other half before the winter crop season. The areas were cultivated with soybean (Glycine max L.) and maize (Zea mays L.) in the summers of 1998 and 1999, respectively, and with wheat (Triticum sativum Lam.) in the winters of both years. Soil samples were collected at 0-5, 5-10, and 10-20 cm depths. Under both CT and NT systems, pig slurry application increased C and N mineralization. However, increasing pig slurry additions decreased the C to N mineralization ratio. Under the NT system, C and N mineralization was greater than in CT system.
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A comment about the article “Local sensitivity analysis for compositional data with application to soil texture in hydrologic modelling” writen by L. Loosvelt and co-authors. The present comment is centered in three specific points. The first one is related to the fact that the authors avoid the use of ilr-coordinates. The second one refers to some generalization of sensitivity analysis when input parameters are compositional. The third tries to show that the role of the Dirichlet distribution in the sensitivity analysis is irrelevant
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Bradyrhizobium japonicum is a symbiotic nitrogen-fixing soil bacteria that induce root nodules formation in legume soybean (Glycine max.). Using (13)C- and (31)P-nuclear magnetic resonance (NMR) spectroscopy, we have analysed the metabolite profiles of cultivated B. japonicum cells and bacteroids isolated from soybean nodules. Our results revealed some quantitative and qualitative differences between the metabolite profiles of bacteroids and their vegetative state. This includes in bacteroids a huge accumulation of soluble carbohydrates such as trehalose, glutamate, myo-inositol and homospermidine as well as Pi, nucleotide pools and intermediates of the primary carbon metabolism. Using this novel approach, these data show that most of the compounds detected in bacteroids reflect the metabolic adaptation of rhizobia to the surrounding microenvironment with its host plant cells.
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The objective of this work was to determine differences in leaf mineral composition between ungrafted and grafted onto (Solanum torvum) eggplant (Solanum melongena), cultivars 'Faselis' and 'Pala', grown in a soil infested with Verticillium dahliae and Meloidogyne incognita, or in a noninfested soil. Grafting increased leaf P and Mn concentrations, and decreased N concentrations, in both soils. Grafting also enhanced leaf Ca concentration of 'Pala', but it did not affect that of 'Faselis' depending on the cropping year. Leaf Mg concentration of grafted plants in infested soil was lower than that of ungrafted ones in noninfested soil. Results showed that, under the same fertilization program, the grafted 'Faselis' plants used the nutrients more efficiently than the 'Pala' ones. Use of S. torvum as a rootstock for 'Faselis' resulted in an effective protection against multiple pathogen infestation. Fertilization may be necessary when grafted 'Faselis' plants are grown in a soil infested with the pathogens, since grafting and infestation generally decrease leaf N, Mg, Ca and Fe concentrations, either by reducing the nutrient concentrations directly or by increasing leaf Mn concentration.
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The water content dynamics in the upper soil surface during evaporation is a key element in land-atmosphere exchanges. Previous experimental studies have suggested that the soil water content increases at the depth of 5 to 15 cm below the soil surface during evapo- ration, while the layer in the immediate vicinity of the soil surface is drying. In this study, the dynamics of water content profiles exposed to solar radiative forcing was monitored at a high temporal resolution using dielectric methods both in the presence and absence of evaporation. A 4-d comparison of reported moisture content in coarse sand in covered and uncovered buckets using a commercial dielectric-based probe (70 MHz ECH2O-5TE, Decagon Devices, Pullman, WA) and the standard 1-GHz time domain reflectometry method. Both sensors reported a positive correlation between temperature and water content in the 5- to 10-cm depth, most pronounced in the morning during heating and in the afternoon during cooling. Such positive correlation might have a physical origin induced by evaporation at the surface and redistribution due to liquid water fluxes resulting from the temperature- gradient dynamics within the sand profile at those depths. Our experimental data suggest that the combined effect of surface evaporation and temperature-gradient dynamics should be considered to analyze experimental soil water profiles. Additional effects related to the frequency of operation and to protocols for temperature compensation of the dielectric sensors may also affect the probes' response during large temperature changes.
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Spatial data analysis mapping and visualization is of great importance in various fields: environment, pollution, natural hazards and risks, epidemiology, spatial econometrics, etc. A basic task of spatial mapping is to make predictions based on some empirical data (measurements). A number of state-of-the-art methods can be used for the task: deterministic interpolations, methods of geostatistics: the family of kriging estimators (Deutsch and Journel, 1997), machine learning algorithms such as artificial neural networks (ANN) of different architectures, hybrid ANN-geostatistics models (Kanevski and Maignan, 2004; Kanevski et al., 1996), etc. All the methods mentioned above can be used for solving the problem of spatial data mapping. Environmental empirical data are always contaminated/corrupted by noise, and often with noise of unknown nature. That's one of the reasons why deterministic models can be inconsistent, since they treat the measurements as values of some unknown function that should be interpolated. Kriging estimators treat the measurements as the realization of some spatial randomn process. To obtain the estimation with kriging one has to model the spatial structure of the data: spatial correlation function or (semi-)variogram. This task can be complicated if there is not sufficient number of measurements and variogram is sensitive to outliers and extremes. ANN is a powerful tool, but it also suffers from the number of reasons. of a special type ? multiplayer perceptrons ? are often used as a detrending tool in hybrid (ANN+geostatistics) models (Kanevski and Maignank, 2004). Therefore, development and adaptation of the method that would be nonlinear and robust to noise in measurements, would deal with the small empirical datasets and which has solid mathematical background is of great importance. The present paper deals with such model, based on Statistical Learning Theory (SLT) - Support Vector Regression. SLT is a general mathematical framework devoted to the problem of estimation of the dependencies from empirical data (Hastie et al, 2004; Vapnik, 1998). SLT models for classification - Support Vector Machines - have shown good results on different machine learning tasks. The results of SVM classification of spatial data are also promising (Kanevski et al, 2002). The properties of SVM for regression - Support Vector Regression (SVR) are less studied. First results of the application of SVR for spatial mapping of physical quantities were obtained by the authorsin for mapping of medium porosity (Kanevski et al, 1999), and for mapping of radioactively contaminated territories (Kanevski and Canu, 2000). The present paper is devoted to further understanding of the properties of SVR model for spatial data analysis and mapping. Detailed description of the SVR theory can be found in (Cristianini and Shawe-Taylor, 2000; Smola, 1996) and basic equations for the nonlinear modeling are given in section 2. Section 3 discusses the application of SVR for spatial data mapping on the real case study - soil pollution by Cs137 radionuclide. Section 4 discusses the properties of the modelapplied to noised data or data with outliers.
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The objective of this work was to assess the effect of different coffee organic cultivation systems on chemical and biological soil characteristics, in different seasons of the year. The following systems were evaluated: coffee intercropped with one (CJ1), two (CJ2) or three (CJ3) pigeon pea (Cajanus cajan) alleys; coffee planted under full sun (CS); area planted with sweet pepper and snap bean in a conventional tillage system (AC); and secondary forest area (FFR). Row spacing in CJ1, CJ2, CJ3 and CS was 2.0x1.0, 2.8x1.0, 3.6x1.0, and 2.8x1.0 m, respectively. Soil samples were collected at 10-cm depth, during the four seasons of the year. The results were subjected to analysis of variance, principal component analysis, and redundancy analysis. There was an increase in edaphic macrofauna, soil basal respiration, and microbial quotient in the summer. Total macrofauna density was greater in CJ2 followed by CJ3, CS, CJ1, AC and FFR; Coleoptera, Formicidae, and Isoptera were the most abundant groups. There are no significant differences among the areas for soil basal respiration, and the metabolic quotient is higher in CJ1, CJ3, and FFR. Microbial biomass carbon and the contents of K, pH, Ca+Mg, and P show greater values in AC.
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The objective of this work was to evaluate the effect of the pasture (Urochloa brizantha) component age on soil biological properties, in a crop-livestock integrated system. The experiment was carried out in a Brazilian savannah (Cerrado) area with 92 ha, divided into six pens of approximately 15 ha. Each pen represented a different stage of the pasture component: formation, P0; one year, P1; two years, P2; three years, P3; and final with 3.5 years, Pf. Samples were taken in the 0-10 cm soil depth. The soil biological parameters - microbial biomass carbon (MBC), microbial biomass respiration (C-CO2), metabolic quotient (qCO2), microbial quotient (q mic), and total organic carbon (TOC) - were evaluated and compared among different stages of the pasture, and between an adjacent area under native Cerrado and another area under degraded pasture (PCD). The MBC, q mic and TOC increased and qCO2 reduced under the different pasture stages. Compared to PCD, the pasture stages had higher MBC, q mic and TOC, and lower qCO2. The crop-livestock integrated system improved soil microbiological parameters and immobilized carbon in the soil in comparison to the degraded pasture.
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The objective of this work was to evaluate the effect of winter land use on the amount of residual straw, the physical soil properties and grain yields of maize, common bean and soybean summer crops cultivated in succession. The experiment was carried out in the North Plateau of Santa Catarina state, Brazil, from May 2006 to April 2010. Five strategies of land use in winter were evaluated: intercropping with black oat + ryegrass + vetch, without grazing and nitrogen (N) fertilization (intercropping cover); the same intercropping, with grazing and 100 kg ha-1 of N per year topdressing (pasture with N); the same intercropping, with grazing and without nitrogen fertilization (pasture without N); oilseed radish, without grazing and nitrogen fertilization (oilseed radish); and natural vegetation, without grazing and nitrogen fertilization (fallow). Intercropping cover produces a greater amount of biomass in the system and, consequently, a greater accumulation of total and particulate organic carbon on the surface soil layer. However, land use in winter does not significantly affect soil physical properties related to soil compaction, nor the grain yield of maize, soybean and common bean cultivated in succession.
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In the search for new soil stabilizing agents the effects of six organic cations on plastic limit, liquid limit, shrinkage limit, air-dry strength and rate of slaking of a highly plastic clay subsoil were studied. In all cases the plasticity index and shrinkage were reduced by the treatments. The air-dry strength was lowered in varying degree, which was the only undesirable effect noted. With one exception resistance to slaking was improved. It is concluded that large organic cations show promise as possible stabilizing agents for highly plastic fine-grained soils.
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The objective of this work was to evaluate the correlation between sugarcane yield and some physical and chemical attributes of soil. For this, a 42‑ha test area in Araras, SP, Brazil, was used. Soil properties were determined from samples collected at the beginning of the 2003/2004 harvest season, using a regular 100x100 m grid. Yield assessment was done with a yield monitor (Simprocana). Correlation analyses were performed between sugarcane yield and the following soil properties: pH, pH CaCl2, N, C, cone index, clay content, soil organic matter, P, K, Ca, Mg, H+AL, cation exchange capacity, and base saturation. Correlation coefficients were respectively ‑0.05, ‑0.29, 0.33, 0.41, ‑0.27, 0.22, 0.44, ‑0.24, trace, ‑0.06, 0.01, 0.32, 0.14, and 0.04. Correlations of chemical and physical attributes of soil with sugarcane yield are weak, and, per se, they are not able to explain sugarcane yield variation, which suggests that other variables, besides soil attributes, should be analysed.
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The objective of this work was to verify whether the addition of biochar to the soil affects the degradation of litter and of soil organic matter (SOM). In order to investigate the effect of biochar on the mineralization of barley straw, soil was incubated with 14C-labelled barley straw with or without unlabelled biochar. To investigate the effect of straw on the mineralization of biochar, soil was incubated with 14C-labelled biochar with or without straw. In addition, to investigate the effect of biochar on old SOM, a soil labelled by applying labelled straw 40 years ago was incubated with different levels of biochar. All experiments had a control treatment, without any soil amendment. The effect of biochar on the straw mineralization was small and nonsignificant. Without biochar, 48±0.2% of the straw carbon was mineralized within the 451 days of the experiment. In comparison, 45±1.6% of C was mineralized after biochar addition of 1.5 g kg-1. In the SOM-labelled soil, the organic matter mineralized more slowly with the increasing doses of biochar. Biochar addition at 7.7 g kg-1 reduced SOM mineralization from 6.6 to 6.3%, during the experimental period. The addition of 15.5 g kg-1 of biochar reduced the mineralized SOM to 5.7%. There is no evidence of increased degradation of either litter or SOM due to biochar addition; consequently, there is no evidence of decreased stability of SOM.
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The objective of this work was to evaluate the effect of biochar made from Eucalyptus on soil fertility, and on the yield and development of upland rice. The experiment was performed during two years in a randomized block design with four replicates, in a sandy loam Dystric Plinthosol. Four doses of NPK 05-25-15, annually distributed in stripes (0, 100, 200 and 300 kg ha-1), and four doses of biochar (0, 8, 16 and 32 Mg ha-1), applied once in the first year - alone or with NPK - were evaluated. In the first year, biochar positively affected soil fertility [total organic carbon (TOC), Ca, P, Al, H+Al, and pH], at 0-10 cm soil depth, and it was the only factor with significant effect on yield. In the second year, the effect of biochar diminished or was overcome by the fertilizer. TOC moved down in the soil profile to the 0-20 cm depth, influencing K availability in this layer. In the second year, there was a significant interaction between biochar and the fertilizer on plant growth and biomass dry matter accumulation.