978 resultados para Shoot
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Agronomia (Horticultura) - FCA
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Estudou-se o efeito da inoculação com fungo micorrízico arbuscular (FMA), Glomus etunicatum, Glomus clarum, ou Scutelospora heterogama, e da adição de fósforo solúvel (15, 30, 45, 60, 120 e 240 mg de P kg-1 de terra) sobre as variáveis altura, número de folhas, diâmetro do caule, massas vegetais aérea e radicular, teores de nutrientes nas folhas e colonização micorrízica no porta-enxerto limoeiro Cravo (Citrus limonia (L.) Osbeck). Os FMAs, Glomus etunicatum e Glomus clarum, e doses crescentes de fósforo exerceram efeitos significativos sobre essas variáveis. O efeito da inoculação sobre as variáveis de crescimento vegetal foi mais acentuado nos tratamentos com adição de 30 a 60 mg de P kg-1 de terra. Para o teor de P foliar, o efeito da inoculação foi mais acentuado nos tratamentos adicionados de 120 a 240 mg de P kg-1 de terra. A inoculação com estes FMAs aumentou a eficiência do limoeiro Cravo em absorver nutrientes, tanto que as variáveis estudadas em plantas inoculadas na ausência de adubação fosfática superaram às de plantas não inoculadas em substrato adicionado de 240 mg de P kg-1 de terra.
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Dendrobium is a large genus in the family Orchidaceae that exhibits vast diversity in floral characteristics, which is of considerable importance to orchid breeders, biotechnologists and collectors. Native species have high value as a result of their medicinal properties, while their hybrids are important as ornamental commodities, either as cut flowers or potted plants and are thus veritable industrial crops. Thus, preservation of Dendrobium germplasm is valuable for species conservation, breeding programs and the floriculture industry. Cryopreservation represents the only safe, efficient and cost-effective long-term storage option to facilitate the conservation of genetic resources of plant species. This review highlights 16 years of literature related to the preservation of Dendrobium germplasm and comprises the most comprehensive assessment of thorough studies performed to date, which shows reliable and reproducible results. Air-drying, encapsulation-dehydration, encapsulation-vitrification, vitrification and droplet-vitrification are the current cryopreservation methodologies that have been used to cryopreserve Dendrobium germplasm. Mature seeds, pollen, protoplasts, shoot primordia, protocorms and somatic embryos or protocorm-like bodies (PLBs) have been cryopreserved with different levels of success. Encapsulation-vitrification and encapsulation-dehydration are the most used protocol, while PLBs represent the main explant explored.
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Pinus taeda is one of the main timber trees in Brazil, occupying 1.8 million ha with an annual productivity of 25-30 m(3) ha(-1). Another important species is Araucaria angustifolia, belonging to the fragile Rainforest biome, which for decades has been a major source of timber in Brazil. Some diseases that affect the roots and/or the stem of these trees and cause "damping-off" of the seedlings, with economic and environmental losses for the forest sector, are caused by the plant pathogenic fungi Fusarium sp. or Armillaria sp. This research project intended to isolate actinobacteria from the Araucaria rhizosphere, which present an antagonistic effect against these fungi. After the selection of the best pathogen inhibitors, morphologic characteristics, enzyme production, and their effect on the growth of Pinus taeda were studied. The actinobacteria were tested for their antagonistic capacity against Fusarium sp. in Petri plates with PDA as substrate. The inhibition zone was measured after 3, 5, 7, and 10 days. Of all the isolates tested, only two of them maintained inhibition zones up to 4 mm for 10 days. The inhibition of Armillaria sp. was tested in liquid medium and also in Petri dishes through the evaluation of the number of the fungal rhizomorphs in dual culture with the actinobacteria. It was found that all five isolates were able to inhibit the rhizomorph production, with the best performance of the isolate A43, which was capable of inhibiting both fungi, Fusarium and Armillaria. In a greenhouse experiment, the effect of five isolates on the growth of Pinus taeda seedlings was tested. Plant height, stem diameter, root and shoot dry matter were determined. The Streptomyces isolate A43 doubled plant growth. These results may lead to the development of new technologies in the identification of still unknown bacterial metabolites and new management techniques to control forest plant diseases.
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The effects of municipal-treated wastewater on growth and gas exchange characteristics were studied in eucalyptus plants cultivated in a greenhouse under continuous sub irrigation with 70 % Long Ashton solution as a control, 100 % treated wastewater, and treated wastewater diluted 50 % with tap water. Irrigation with wastewater resulted in a reduction of total dry matter per plant and the shoot-to-root ratio and increased specific leaf mass, irrespective of the dilution. The gas exchange characteristics were adversely affected by the irrigation with wastewater, with photosynthetic rates been negatively affected. Total chlorophyll and carotenoids content were reduced in plants grown under treated wastewater. The results demonstrated that when the eucalyptus plants were grown under treated wastewater as the only source of mineral nutrients they were able to produce nearly 50 % of the dry matter produced by the plants grown under appropriate mineral nutrient supply, irrespective of the dilution. Therefore, the contribution of mineral nutrients and organic matter from the treated wastewater used as agricultural irrigation will be significant in lower the fertilizer rates without reducing dry matter production per plant.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Soil that has a high degree of weathering, with more inorganic P bound to Fe and Al oxides, has less P availability to plants. Thus, the critical element of a plant refers to the level below which the growth rate and plant production decreases, demonstrating the need for supplementary fertilization. Accordingly, an experiment was conducted in a greenhouse at Embrapa Algodao with the objective of evaluating the response of castor plants to five doses of P in four types of soil with different adsorption characteristics and critical levels of foliar P. The experimental design was completely randomized in a 4 x 5 factorial design, four types of soil and 5 levels of P with four replications. For TCo, there was an increase in height growth, with dose of 229.6 mg dm(-3) responsible for maximum plant height (74.3 cm). The largest diameter stem (17.58 mm) was observed in CXve with an application of 229.6 mg dm(-3) of P; a decrease was seen when using higher doses. The increase in leaf area was smaller in RY (4724.8 cm(2)), where it was obtained with a dose of 280.2 mg dm(-3). In general, critical levels of P in the plant shoots did not vary much between the soils. The critical level of P in castor bean shoot dry mass was higher (4.61 g kg(-1)) in TCo, this result being directly related to the low clay content of the soil.
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The use of biostimulants can alter plant growth and development, but the action of they may be varied according to the stage of development of the plant. The aim was to evaluate the effects of forms and times of a biostimulant (cytokinin, indolebutyric acid, and gibberellic acid) application on nodulation, some biochemical aspects, growth and yield of common bean cultivar Perola. The treatments were: Control (without application); TS - 250 mL ha(-1) seed treatment; V-4 - 250 mL ha(-1) foliar spray in V-4 stage; R-5 - 250 mL ha(-1) foliar spray in R-5 stage; TS+V-4 - 250 mL ha(-1) in TS + 250 mL ha(-1) in V-4; TS+R-5 - 250 mL ha(-1) in TS + 250 mL ha(-1) in R-5; V-4+R-5 - 250 mL ha(-1) in V-4 + 250 mL ha(-1) in R-5, and TS+V-4+R-5 - 250 mL ha(-1) in TS + 250 mL ha(-1) in V-4 + 250 mL ha(-1) in R-5. The foliar biostimulant application in the vegetative (V-4) or early reproductive phase (R-5) increases nodulation, root growth, content of soluble sugars, content of total amino acid and nitrate reductase activity, however, does not interfere with shoot growth and grain yield of common bean.
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Despite the high nutritional requirements of Tifton 85 grass, often the importance of liming and potassium fertilization for this forage has been neglected. In order to evaluate the effects of such practices on shoots dry matter and tillering of this forage, was carried out an experiment in green-house conditions, by two cuts, using samples of an Oxisol medium texture and a sandy soil. Efforts were also made to determine parameters to support recommendations for liming and potassium fertilization. Was adopted the completely randomized design with four replications in a factorial scheme 4x4x2, with four K rates (0, 60, 120 and 180 mg kg(-1) K), four pH values (original, 4.9, 5.9 and 6.4) and two soils. Potassium fertilization and liming promoted a significant increase in shoot dry matter and tillering in Tifton 85 grass, in both soils and cuts. The highest yields of shoot dry matter were associated with a base saturation of 56% and pH 5.2. The critical levels of K in soil and plant were 1.7 mmol dm(-3) and 14.0 g kg(-1), respectively.
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This study aimed to evaluate the efficiency of protective fluxofenim seed treatment of sorghum hybrids DKB510 and SCG340, in order to increase the selectivity to the herbicide S-metolachlor applied pre-emergence and to determine the activity of detoxification enzyme glutathione S-transferase (GST). This work was divided into two stages. The first step consisted of field evaluation of the effectiveness of the shield to reduce visual symptoms of plant injury caused by the herbicide and the second determined the activity of GST. It was compared the susceptibility of sorghum seeds to the herbicide by means of visual assessment of injuries at 3, 7, 15 and 30 days after emergence (DAE), root dry weight and shoot at 10 DAE, and determination of activity GST. The treatments were: application of the safener dose at 0 and 40 mL per 100 kg of seed, and spraying of the herbicide S-metolachlor at rates of 1,440 and 2,880 g a.i. ha(-1), and a control without herbicide. The safener use to seed treatment for both sorghum hybrids (DKB510 and SCG340) increased tolerance to the herbicide S-metolachlor in two doses, and the best results were obtained at a dose of 1,440 g ha(-1). The GST enzyme activity showed an increase when using the fluxofenim prior to application of the herbicide S-metolachlor at a dose of 1,440 g ha(-1) for the two hybrids.
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Pós-graduação em Agronomia (Produção Vegetal) - FCAV