Efeitos da corticoterapia pré-natal e durante a puberdade sobre a morfofisiologia do lobo ventral da próstata de ratos senis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mechanical strain induces the production of spheroid mineralized microparticles in the aortic valve through a RhoA/ROCK-dependent mechanism

Calcific aortic valve disease (CAVD) is a chronic disorder characterized by an abnormal mineralization of the leaflets, which is accelerated in bicuspid aortic valve (BAV). It is suspected that mechanical strain may promote/enhance mineralization of the aortic valve. However, the effect of mechanical strain and the involved pathways during mineralization of the aortic valve remains largely unknown. Valve interstitial cells (VICs) were isolated and studied under strain conditions. Human bicuspid aortic valves were examined as a model relevant to increase mechanical strain. Cyclic strain increased mineralization of VICs by several-fold. Scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analyses revealed that mechanical strain promoted the formation of mineralized spheroid microparticles, which coalesced into larger structure at the surface of apoptotic VICs. Apoptosis and mineralization were closely associated with expression of ENPP1. Inhibition of ENPP1 greatly reduced mineralization of VIC cultures. Through several lines of evidence we showed that mechanical strain promoted the export of ENPP1-containing vesicles to the plasma membrane through a RhoA/ROCK pathway. Studies conducted in human BAV revealed the presence of spheroid mineralized structures along with the expression of ENPP1 in areas of high mechanical strain. Mechanical strain promotes the production and accumulation of spheroid mineralized microparticles by VICs, which may represent one important underlying mechanism involved in aortic valve mineralization. RhoA/ROCK-mediated export of ENPP1 to the plasma membrane promotes strain-induced mineralization of VICs.

Caracterização da cinza de bagaço de cana-de-açúcar e aplicação no solo

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Potencial de uso agrícola de levedura seca inativa de cana-de-açúcar e de resíduos orgânicos

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Mineralização de nitrogênio do esterco bovino e produção de alface em função de N-ureia

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Ocorrência de nitrato nas águas subterrâneas do Sistema Aquífero Bauru em áreas rurais

Pós-graduação em Geociências e Meio Ambiente - IGCE

Influência da diabetes mellitus na resposta tecidual e na capacidade de mineralização dos cimentos Sealapex, MTA Angelus e MTA Fillapex

Pós-graduação em Ciência Odontólogica - FOA

Efeito da administração de alendronato, a longo prazo, sobre as propriedades mecânicas do tecido ósseo, sua reparação e mineralização ao redor de implantes osseointegráveis. Estudo em ratas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação da membrana de látex natural (NRL) como suporte para cultura das células osteogênicas MC3T3-E1 para regeneração óssea guiada (GBR)

Pós-graduação em Biotecnologia - IQ

Estudo da atividade das enzimas envolvidas na ossificação de frangos de corte normais e acometidos por discondroplasia tibial

Pós-graduação em Biotecnologia - IQ

Cytotoxicity of dimethyl sulfoxide (DMSO) in direct contact with odontoblast-like cells

To evaluate the cytotoxicity of dimethyl sulfoxide (DMSO) on the repair-related activity of cultured odontoblast-like MDPC-23 cells. Methods Solutions with different concentrations of DMSO (0.05, 0.1, 0.3, 0.5 and 1.0 mM), diluted in culture medium (DMEM), were placed in contact with MDPC-23 cells (5 × 104 cells/cm2) for 24 h. Eight replicates (n = 8) were prepared for each solutions for the following methods of analysis: violet crystal dye for cell adhesion (CA), quantification of total protein (TP), alizarin red for mineralization nodules formation (MN) and cell death by necrosis (flow cytometry); while twelve replicates (n = 12) were prepared for viable cell number (Trypan Blue) and cell viability (MTT assay). Data were analyzed by ANOVA and Tukey or Kruskal–Wallis and Mann–Whitney's tests (p < 0.05). Results Cell viability, adhesion and percentage of cell death by necrosis were not affected by DMSO at any concentration, with no statistical significant difference among the groups. A significant reduction in total protein production was observed for 0.5 and 1.0 mM of DMSO compared to the control while increased mineralized nodules formation was seen only for 1.0 mM DMSO. Significance: DMSO caused no or minor cytotoxic effects on the pulp tissue repair-related activity of odontoblast-like cells.

Portland cement use in dental root perforations: a long term followup

Root canal and furcal perforations are causes of endodontic therapy failure and different materials that stimulate tissue mineralization have been proposed for perforation treatment. In the first case, a patient presented tooth 46 with unsatisfactory endodontic treatment and a periapical radiographic lesion. A radiolucent area compatible with a perforating internal resorption cavity was found in the mesial root. The granulation tissue was removed, and root canals were prepared. The intracanal medication was composed of calcium hydroxide and the perforation cavity was filled with Portland cement. The 11-year followup showed radiographic repair of the tissue adjacent to the perforation and absence of clinical signs and symptoms or periapical lesion. In the second case, a patient presented with edema on the buccal surface of tooth 46. The examination showed a radiolucent area in the furcation region compatible with an iatrogenic perforation cavity. The mesial root canals were calcified, and only the distal root canal was prepared. The cavity was filled with a calcium hydroxide-based paste and the distal root canal was obturated. In sequence, the perforation cavity was filled with Portland cement. The 9-year followup showed the tooth in masticatory function with radiographic and clinical aspects compatible with normality.

Avaliação de fitases microbianas em dietas de frangos de corte

Pós-graduação em Zootecnia - FCAV

Efeitos do treinamento concorrente sobre a composição corporal e gasto energético de repouso em mulheres na menopausa

Pós-graduação em Fisioterapia - FCT

Elementos estruturais relacionados com a mineralização do eoceno - oligoceno entre Mollebamba e Santo Domingo região de Apurimac, sul do Peru

Pós-graduação em Geociências e Meio Ambiente - IGCE