The canine hair cycle - a guide for the assessment of morphological and immunohistochemical criteria

The hair follicle has a lifelong capacity to cycle through recurrent phases of controlled growth (anagen), regression (catagen) and quiescence (telogen), each associated with specific morphological changes. A comprehensive classification scheme is available for mice to distinguish the cycle stages anagen I-VI, catagen I-VIII and telogen. For dogs, such a classification system does not exist, although alopecia associated with hair cycle arrest is common. We applied analogous morphological criteria and various staining techniques to subdivide the canine hair cycle stages to the same extent as has been done in mice. Of all the staining techniques applied, haematoxylin and eosin stain, Sacpic, Masson Fontana and immunohistochemistry for vimentin and laminin proved to be most useful. To evaluate the applicability of our criteria, we investigated skin biopsies from healthy beagle dogs (n=20; biopsies from shoulder and thigh) kept in controlled conditions. From each biopsy, at least 50 hair follicles were assessed. Statistical analysis revealed that 30% of the follicles were in anagen (12% early and 18% late), 8% in catagen (2% early, 5% late and 1% not determinable) and 27% in telogen. Thirty-five per cent of hair follicles could not be assigned to a specific cycle stage because not all follicles within one biopsy were oriented perfectly. In conclusion, this guide will not only be helpful for the investigation of alopecic disorders and possibly their pathogenesis, but may also serve as a basis for research projects in which the comparison of hair cycle stages is essential, e.g. comparative analysis of gene expression patterns.

Essential hypertension - which genes can be held responsible for it?

Hypertension is a common heritable cardiovascular risk factor. Some rare monogenic forms of hypertension have been described, but the majority of patients suffer from essential hypertension, for whom the underlying genetic mechanisms are not clear. Essential hypertension is a complex trait, involving multiple genes and environmental factors. Recently, progress in the identification of common genetic variants associated with essential hypertension has been made due to large-scale international collaborative projects. In this article we review the new research methods used as well as selected recent findings in this field.

Nongovernmental organization staff views of global water privatization

More than 1 billion people lack access to clean water and proper sanitation. As part of efforts to solve this problem, there is a growing shift from public to private water management led by The World Bank and the International Monetary Fund (IMF). This shift has inspired much related research. Researchers have assessed water privatization related perceptions of consumers, government officials, and multinational company agents. This thesis presents results of a study of nongovernmental (NGO) staff perceptions of water privatization. Although NGOs are important actors in sustainable water related development through water provision, we have little understanding of their perceptions of water privatization and how it impacts their activities. My goal was to fill this gap. I sampled international and national development NGOs with water, sanitation, and hygiene (WASH) foci. I conducted 28 interviews between January and June of 2011 with staff in key positions including water policy analysts, program officers, and project coordinators. Their perceptions of water privatization were mixed. I also found that local water privatization in most cases does not influence NGO decisions to conduct projects in a region. I found that development NGO staff base their beliefs about water privatization on a mix of personal experience and media coverage. My findings have important implications for the WASH sector as we work to solve the worsening global water access crisis.

SWISSspine: a nationwide registry for health technology assessment of lumbar disc prostheses

SWISSspine is a so-called pragmatic trial for assessment of safety and efficiency of total disc arthroplasty (TDA). It follows the new health technology assessment (HTA) principle of "coverage with evidence development". It is the first mandatory HTA registry of its kind in the history of Swiss orthopaedic surgery. Its goal is the generation of evidence for a decision by the Swiss federal office of health about reimbursement of the concerned technologies and treatments by the basic health insurance of Switzerland. During the time between March 2005 and 2008, 427 interventions with implantation of 497 lumbar total disc arthroplasties have been documented. Data was collected in a prospective, observational multicenter mode. The preliminary timeframe for the registry was 3 years and has already been extended. Data collection happens pre- and perioperatively, at the 3 months and 1-year follow-up and annually thereafter. Surgery, implant and follow-up case report forms are administered by spinal surgeons. Comorbidity questionnaires, NASS and EQ-5D forms are completed by the patients. Significant and clinically relevant reduction of low back pain VAS (70.3-29.4 points preop to 1-year postop, p < 0.0001) leg pain VAS (55.5-19.1 points preop to 1-year postop, p < 0.001), improvement of quality of life (EQ-5D, 0.32-0.73 points preop to 1-year postop, p < 0.001) and reduction of pain killer consumption was revealed at the 1-year follow-up. There were 14 (3.9%) complications and 7 (2.0%) revisions within the same hospitalization reported for monosegmental TDA; there were 6 (8.6%) complications and 8 (11.4%) revisions for bisegmental surgery. There were 35 patients (9.8%) with complications during followup in monosegmental and 9 (12.9%) in bisegmental surgery and 11 (3.1%) revisions with 1 [corrected] new hospitalization in monosegmental and 1 (1.4%) in bisegmental surgery. Regression analysis suggested a preoperative VAS "threshold value" of about 44 points for increased likelihood of a minimum clinically relevant back pain improvement. In a short-term perspective, lumbar TDA appears as a relatively safe and efficient procedure concerning pain reduction and improvement of quality of life. Nevertheless, no prediction about the long-term goals of TDA can be made yet. The SWISSspine registry proofs to be an excellent tool for collection of observational data in a nationwide framework whereby advantages and deficits of its design must be considered. It can act as a model for similar projects in other health-care domains.

Potentials and limitations of coordinated spatial and non-spatial information

Information management and geoinformation systems (GIS) have become indispensable in a large majority of protected areas all over the world. These tools are used for management purposes as well as for research and in recent years have become even more important for visitor information, education and communication. This study is divided into two parts: the first part provides a general overview of GIS and information management in a selected number of national park organizations. The second part lists and evaluates the needs of evolving large protected areas in Switzerland. The results show a wide use of GIS and information management tools in well established protected areas. The more isolated use of singular GIS tools has increasingly been replaced by an integrated geoinformation management. However, interview partners pointed out that human resources for GIS in most parks are limited. The interviews also highlight uneven access to national geodata. The view of integrated geoinformation management is not yet fully developed in the park projects in Switzerland. Short-term needs, such as software and data availability, motivate a large number of responses collected within an exhaustive questionnaire. Nevertheless, the need for coordinated action has been identified and should be followed up. The park organizations in North America show how an effective coordination and cooperation might be organized.

ARTHUR: A Collaborative Augmented Environment for Architectural Design and Urban Planning

Projects in the area of architectural design and urban planning typically engage several architects as well as experts from other professions. While the design and review meetings thus often involve a large number of cooperating participants, the actual design is still done by the individuals in the time in between those meetings using desktop PCs and CAD applications. A real collaborative approach to architectural design and urban planning is often limited to early paper-based sketches.In order to overcome these limitations, we designed and realized the ARTHUR system, an Augmented Reality (AR) enhanced round table to support complex design and planning decisions for architects. WhileAR has been applied to this area earlier, our approach does not try to replace the use of CAD systems but rather integrates them seamlessly into the collaborative AR environment. The approach is enhanced by intuitiveinteraction mechanisms that can be easily con-figured for different application scenarios.

Low-Income Developing Countries and WTO Litigation: Why Wake Up the Sleeping Dog?

The World Trade Organization (WTO) is one of the most judicialized dispute settlement systems in international politics. While a general appreciation has developed that the system has worked quite well, research has not paid sufficient attention to the weakest actors in the system. This paper addresses the puzzle of missing cases of least-developed countries initiating WTO disputes settlement procedures. It challenges the existing literature on developing countries in WTO dispute settlement which predominantly focuses on legal capacity and economic interests. The paper provides an argument that the small universe of ‘actionable cases’, the option of free riding and the assessment of the perceived opportunity costs related to other foreign policy priorities better explain the absence of cases. In addition (and somewhat counterintuitively), we argue that the absence of cases is not necessarily bad news and shows how the weakest actors can use the dispute settlement system in a ‘lighter version’ or in indirect ways. The argument is empirically assessed by conducting a case study on four West African cotton-producing countries (C4) and their involvement in dispute settlement.

Raw as Archival Still Image Format: A Consideration

Source materials like fine art, over-sized, fragile maps, and delicate artifacts have traditionally been digitally converted through the use of controlled lighting and high resolution scanners and camera backs. In addition the capture of items such as general and special collections bound monographs has recently grown both through consortial efforts like the Internet Archive's Open Content Alliance and locally at the individual institution level. These projects, in turn, have introduced increasingly higher resolution consumer-grade digital single lens reflex cameras or "DSLRs" as a significant part of the general cultural heritage digital conversion workflow. Central to the authors' discussion is the fact that both camera backs and DSLRs commonly share the ability to capture native raw file formats. Because these formats include such advantages as access to an image's raw mosaic sensor data within their architecture, many institutions choose raw for initial capture due to its high bit-level and unprocessed nature. However to date these same raw formats, so important to many at the point of capture, have yet to be considered "archival" within most published still imaging standards, if they are considered at all. Throughout many workflows raw files are deleted and thrown away after more traditionally "archival" uncompressed TIFF or JPEG 2000 files have been derived downstream from their raw source formats [1][2]. As a result, the authors examine the nature of raw anew and consider the basic questions, Should raw files be retained? What might their role be? Might they in fact form a new archival format space? Included in the discussion is a survey of assorted raw file types and their attributes. Also addressed are various sustainability issues as they pertain to archival formats with a special emphasis on both raw's positive and negative characteristics as they apply to archival practices. Current common archival workflows versus possible raw-based ones are investigated as well. These comparisons are noted in the context of each approach's differing levels of usable captured image data, various preservation virtues, and the divergent ideas of strictly fixed renditions versus the potential for improved renditions over time. Special attention is given to the DNG raw format through a detailed inspection of a number of its various structural components and the roles that they play in the format's latest specification. Finally an evaluation is drawn of both proprietary raw formats in general and DNG in particular as possible alternative archival formats for still imaging.

Towards Faster Method Search Through Static Ecosystem Analysis

Software developers are often unsure of the exact name of the method they need to use to invoke the desired behavior in a given context. This results in a process of searching for the correct method name in documentation, which can be lengthy and distracting to the developer. We can decrease the method search time by enhancing the documentation of a class with the most frequently used methods. Usage frequency data for methods is gathered by analyzing other projects from the same ecosystem - written in the same language and sharing dependencies. We implemented a proof of concept of the approach for Pharo Smalltalk and Java. In Pharo Smalltalk, methods are commonly searched for using a code browser tool called "Nautilus", and in Java using a web browser displaying HTML based documentation - Javadoc. We developed plugins for both browsers and gathered method usage data from open source projects, in order to increase developer productivity by reducing method search time. A small initial evaluation has been conducted showing promising results in improving developer productivity.

Human rights and international trade

Economic globalization and respect for human rights are both highly topical issues. In theory, more trade should increase economic welfare and protection of human rights should ensure individual dignity. Both fields of law protect certain freedoms: economic development should lead to higher human rights standards, and UN embargoes are used to secure compliance with human rights agreements. However the interaction between trade liberalisation and human rights protection is complex, and recently, tension has arisen between these two areas. Do WTO obligations covering intellectual property prevent governments from implementing their human rights obligations, including rights to food or health? Is it fair to accord the benefits of trade subject to a clean human rights record? This book first examines the theoretical framework of the interaction between the disciplines of international trade law and human rights. It builds upon the well-known debate between Professor Ernst-Ulrich Petersmann, who construes trade obligations as human rights, and Professor Philip Alston, who warns of a merger and acquisition of human rights by trade law. From this starting point, further chapters explore the differing legal matrices of the two fields and examine how cooperation between them might be improved, both in international law-making and institutions,in dispute settlement. The interaction between trade and human rights is then explored through seven case studies:freedom of expression and competition law; IP protection and health; agricultural trade and the right to food; trade restrictions on conflict WHO convention on tobacco control; and, finally, human rights conditionalities in preferential trade schemes.

A review of the Malaria Chemoprophylaxis Compliance Program at ExxonMobil Corporation

ExxonMobil, a Fortune 500 oil and gas corporation, has a global workforce with employees assigned to projects in areas at risk for infectious diseases, particularly malaria. As such, the corporation has put in place a program to protect the health of workers and ensure their safety in malaria endemic zones. This program is called the Malaria Control Program (MCP). One component of this program is the more specific Malaria Chemoprophylaxis Compliance Program (MCCP), in which employees enroll following consent to random drug testing for compliance with the company's chemoprophylaxis requirements. Each year, data is gathered on the number of employees working in these locations and are selected randomly and tested for chemoprophylaxis compliance. The selection strives to test each eligible worker once per year. Test results that come back positive for the chemoprophylaxis drug are considered "detects" and tests that are negative for the drug and therefore show the worker is non-compliant at risk for severe malaria infection are considered "non-detect". ^ The current practice report used aggregate data to calculate statistics on test results to reflect compliance among both employees and contractors in various malaria-endemic areas. This aggregate, non-individualized data has been compiled and reflects the effectiveness and reach of ExxonMobil's Malaria Chemoprophylaxis Compliance Program. In order to assess compliance, information on the number of non-detect test results was compared to the number of tests completed per year. The data shows that over time, non-detect results have declined in both employee and contractor populations, and vary somewhat by location due to size and scope of the MCCP implemented in-country. Although the data indicate a positive trend for the corporation, some recommendations have been made for future implementation of the program.^

Los grandes proyectos de infraestructura del Mercosur : la hidrovía Paraguay-Paraná : realidades y controversias

El presente trabajo tiene como objetivo demostrar la importancia de la hidrovía Paraná‐Paraguay dentro del contexto del Mercosur. Esta obra de infraestructura es un elemento clave para la integración física efectiva, ya que permite expandir el desarrollo comercial de la región, reduciendo los costos de transporte. Por sus características, la hidrovía es una de las principales vías fluviales de navegación del mundo, tanto por su longitud como por su caudal. Los gobiernos tanto de Argentina como de Brasil y Paraguay, impulsaron la expansión de la hidrovía Paraguay‐ Paraná, porque lo consideraron un dinamizador potencial de las economías regionales. Los países del Mercosur invirtieron, y probablemente seguirán invirtiendo miles de millones de dólares en la ampliación y modernización de su infraestructura portuaria en los próximos años, más aún cuando consideren que la profundización de la integración también pasa, en gran parte, por la integración hídrica. Dado que la hidrovía es financiada por el sector público, sector privado y organismos internacionales de crédito, sumado ello al controvertido impacto ambiental de las obras, se han generado debates y resistencia al avance de las obras, por parte de organismos no gubernamentales dedicados al medio ambiente. Sin embargo, este debate todavía está inconcluso, así también como las obras, que todavía se encuentran en proyecto de ejecución.

Miradas que interpelan la intervención social : repensando las trayectorias del Trabajo Social en torno a la formación académica, la producción de conocimientos y los proyectos ético-políticos

El artículo presenta algunos resultados acerca de la investigación sobre la construcción de proyectos ético-políticos y su relación con las políticas sociales y la reconfiguración de lo social. Pretende aportar a la comprensión del Trabajo Social como sujeto colectivo y al análisis de la incidencia de dichos proyectos en la formación profesional y en la producción de conocimientos) como modos de interpelación a la intervención social desde diversas miradas. Debatir acerca de la actuación profesional del Trabajo Social supone cuestionar la formación que debe brindar la universidad teniendo en cuenta los atravesamientos socio-políticos y las transformaciones societales, desde una mirada socio-histórica que permita reconocer y valorar su trayectoria y los aspectos emergentes.

Los caminos de la construcción de una vivienda popular : caracterizaciones socioculturales en el espacio doméstico en Buenos Aires, hasta la década de 1940

Desde fines del siglo XIX, en diferentes ámbitos institucionales, científicos, y profesionales, se comenzó a dirimir sobre la construcción de viviendas por parte del Estado destinadas a los sectores y grupos sociales identificados como empleados, pobres, trabajadores, obreros, e inmigrantes, a la vez que se establecían correspondencias entre los niveles socioculturales y socioeconómicos, las localizaciones urbanas y sus espacios domésticos. Las propuestas oscilaban entre la aceptación, transformación, y erradicación, y hacían evidente los juicios de valor divergentes sobre la coexistencia de grupos y sectores sociales diversos en el medio urbano, hasta arribar en la década de 1930 a concepciones conciliadoras que comenzaron a operar bajo el signo de lo popular. Se analizarán los discursos sobre el espacio doméstico que emergieron en los contextos de difusión y discusión de los diferentes proyectos habitacionales legislativos y arquitecturales con la finalidad de dar cuenta acerca de las caracterizaciones sociales que operaban en cada escenario histórico.

Expansión hidroeléctrica, Estado y economías campesinas : El caso de la represa del Quimbo, Huila-Colombia

En el presente trabajo buscamos contextualizar la situación del sector hidroeléctrico en América Latina, los principales argumentos que se presentan por parte de diferentes actores para la implementación de estos proyectos y la realidad que esto ofrece para las economías locales impactadas. En primer lugar, se presenta la configuración en América Latina de las principales iniciativas de expansión del sector eléctrico, reflejada en la acción de empresas y gobiernos en esta región. Posteriormente, se delimita el artículo a partir del estudio de caso de la hidroeléctrica El Quimbo en Colombia, que muestra la forma de acción del Estado colombiano al impulsar estos proyectos de expansión. Dentro de los procedimientos legales para la implementación de estos proyectos, guarda especial importancia el análisis de la Licencia Ambiental, que refleja en su proceso de concesión diferentes disputas entre los actores relacionados: la empresa Endesa-Enel, los afectados que constituyen diferentes grupos, las diferentes instituciones del Estado y el movimiento social. Metodológicamente, el estudio se basa en una serie de autores que buscan rescatar las contradicciones y los intereses de los afectados por la implementación de este tipo de proyectos (Sigaud 1992, 1995, Vianna 2009, Borralho, 1988) a partir de una muestra de argumentación cualitativa. De este modo, el estudio se sustentó en una revisión bibliográfica y de prensa sobre el funcionamiento de la expansión eléctrica en América Latina y posteriormente sobre el contexto colombiano a partir de sus proyectos de expansión hidroeléctrica y la legislación acerca del funcionamiento de la Licencia Ambiental en este país. Finalmente, se sitúa en el caso concreto de El Quimbo, donde se realizaron diversos trabajos de campo con campesinos localizados en el área de afectación y se recolectó material de prensa y material emitido tanto por Emgesa como por la Asociación de Afectados por la hidroeléctrica El Quimbo-Asoquimbo. El trabajo investigativo refleja el panorama sobre la expansión de grandes proyectos de infraestructura y el consenso que existe sobre su implementación tanto por el sector privado como por los gobiernos nacionales, materializados a través de marcos legales del Estado y figuras como las licencias ambientales. A partir de este caso, se muestra cómo estas decisiones del Estado generan cambios en las condiciones previas de vida de poblaciones rurales, que reaccionan a partir de protestas y de la conformación de grupos con diferentes intereses y tiempos de resistencia y negociación.