Caracterização molecular do gene que codifica a TCTP (Translationally Controlled Tumor Protein) em tomate (Solanum lycopersicum cv. Santa Clara)

The gene encoding TCTP (Translationally Controlled Tumour Protein) is present in all eukaryotes and its product is involved in various cellular processes. Although well characterized in mammals, there are only few works available in the literature related to the analysis of this protein in plants. In this present work, the expression of the gene encoding TCTP was analyzed in different organs/tissues of tomato plants (Solanum lycopersicum cv. Santa Clara). A quantification performed by RT-qPCR revealed the presence of TCTP transcript in all tissues/organs analyzed, with the highest expression level found in leaves. With the exception of fruits in intermediate stage of maturation, for which a small increase on the expression was detected, there was minimal variation in the relative expression of TCTP in other organ/tissues. In parallel, the effects of the constitutive expression of TCTP were investigated using transgenic tobacco lines able to overexpress this protein at different levels (T1, T2 and T3). Seedlings of these lines, and of a non-transgenic control line, were grown in MS culture medium for 21 days. At the end of this period, the length of roots and leaves was taken and the seedlings were photographed. According to Tukey's test, the analysis of the mean root length revealed a significant difference between T1 and T3 lines when compared to the control, although the same was not observed for the T2 line. For leaves, according to Kruskal-Wallis test, there was a statistical difference between the averages of leaf growth obtained for the different lines evaluated. According to these results, we can conclude that TCTP shows an ubiquitous expression in tomato plants, with the highest expression detected in leaves, and also that its overexpression promoted a higher root and leaf development in two of three transgenic tobacco lines tested

Estudo de um caso de tratamento de câncer com um produto alternativo às técnicas convencionais

This work consists of a case study, a qualitative methodology, which aims to show the effects of TIVALLEC, a supplement in vitamins, as an alternative treatment of cancer. This study had also the objective of evaluating one patient’s quality of life thirteen years after being diagnosed with an advanced prostate cancer, and having decided for an alternative treatment using TIVALLEC instead of conventional treatments, having obtained excellent results. An open interview was conducted with patient, evaluating various aspects of his life after the initial diagnosis and the chosen treatment. The answers were transcribed after being limitedly edited for readability. At the end of the study, with the support of recent literature, the interview allowed the conclusion that TIVALLEC was greatly effective, creating good quality of life and wellness for the patient during all the period of thirteen year after the diagnosis up to now. These alternative treatment results may be seen as demanding new studies to help cancer patients who embrace or not the conventional treatments

Estudo de caso: formulação de custo de um produto de usinagem em uma pequena empresa

Many variables are indirectly involved in the transformation of raw material into a metallurgical industry, such as machine hours, hours of hand labor directly and / or indirect, setup time, etc. This research focuses on the complexity of formulating the cost of metallurgical products, based on a case study, in which one has incurred a large loss on the sale of a product called Punch. The main objective of this work is to define the variables of the cost of members and other metallurgical products, so simplified general, checking what were the failures costing the case studied, in order to help others. The method of formulating cost was determined as recommended by SEBRAE guidance for small businesses. The results showed a loss R$ 13.201,00 in the batch of 15 units of punch. Possible improvements have been identified for reducing the production cost

Revista apart: além do conceitual

This magazine aims to be an environment for discussion and information about performance art in Brazil and abroad, taking advantage of the momentum of this art form today. The magazine apart will be the first Brazilian publication to focus on performance art out of academic environments. The collaboration of professionals and scholars lends credibility to the product becoming a benchmark in the study of contemporary art. Besides being an enhancer and exhibitor of artists and works, this magazine also wants to be a work of art itself

Avaliação e comparação do perfil de dissolução do Fluconazol 150mg cápsulas produto referência, genérico e similar

Fármacos com alta permeabilidade intestinal, ampla faixa terapêutica e ausência de evidências documentadas de bioinequivalência ou problemas de biodisponibilidade devido à formulação, foram considerados bioisentos pela ANVISA (RDC 37/2011), ou seja, os fármacos que se encaixam nesta categoria não necessitam de estudos de biodisponibilidade relativa/bioequivalência para serem comercializados. Dentre os catorze produtos que receberam a bioisenção da ANVISA, encontra-se o fluconazol, antifúngico aprovado atualmente para ser comercializado em vários países, inclusive no Brasil, onde inúmeras opções comerciais do produto fluconazol cápsulas, genérico ou similar, podem ser adquiridas. Tendo em vista a bioisenção do produto fluconazol cápsulas e sua larga utilização no mercado brasileiro, o estudo do perfil de dissolução deste produto torna-se relevante. Sendo assim, este trabalho avaliou 2 produtos genéricos e 2 produtos similares frente ao medicamento referência em relação aos seus perfis de dissolução e a similaridade entre eles. Os resultados foram avaliados seguindo a RDC 31/2011 da ANVISA que prevê o cálculo do fator de similaridade entre os perfis de dissolução. E, pôde-se observar que apenas 1 medicamento genérico apresentou o fator de similaridade adequado (entre 50 e 100), sendo 51,60, os demais medicamentos testes apresentaram valores fora do intervalo adotado pela ANVISA. Através desses resultados, conclui-se que intercambialidade do medicamento referência pelo medicamento genérico ou similar não é adequada, uma vez que eles não são considerados iguais, salvo um dos medicamentos genéricos.

Da questão agrária brasileira: uma análise sobre o debate da década de 60 e 70

This monograph object of analysis is the agriculture modernization in Brazil between 1960 and 1980, highlighting the discussion of the economic and politic ideas around the Brazilian agrarian question. The first parte presents an introduction about this issue and the theoretical positions, as well as social and economic implications for the development of the productive forces on rural areas. Nevertheless, reflections of its development to the economy as a whole, highlighting the way the income distribution and concentration was realized. On the second part its presented the agriculture modernization Project on the centralized militar government point of view, by the propositions of that time Agriculture Minister, Antônio Delfim Netto. The approach is made through the proposals precreeded by the Rural Worker Statute and the Land Statute, before the Military Coup, with its news counterpoints settled in law by the Agriculture Modernization Project installed in 1964; the concessions of funding grants and subsidized credits; the relationship between the authoritarian State and the militarization of the land conflicts. The third part deals with the salary dinamics, income and profit on the modernized rural economy created by the agroindustrial productions system. Lastly the conclusion cross the economic and social results of the agrarian question evolution, to the Brazilian agriculture modernization on income distribution

O debate modernidade / pós-modernidade e a crise socioambiental: um estudo sobre teses e dissertações brasileiras em educação ambiental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resistência de cultivares de milho convencional e transgênico à Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae)

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Situação Espacial de Franquias na América do Sul: morfologia e centralidade urbanas em cidades médias da Argentina, Brasil e Chile

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Afirmação do projeto ético-político do serviço social: a utilização das técnicas das manifestações artístico-culturais no debate sobre os movimentos sociais

Pós-graduação em Serviço Social - FCHS

A apropriação das obras de Ricardo Antunes pelo Serviço Social: a categoria trabalho em debate

Pós-graduação em Serviço Social - FCHS

Ciências humanas em debate

Fruto de um arrojado projeto coletivo, este livro representa o trabalho de um grupo de docentes da Unesp e também de outras universidades, que impuseram-se o desafio de refletir sobre a pesquisa em subáreas das Humanidades. Entre as inúmeras questões que eles debateram ganharam destaque as relativas a demandas vitais para o fortalecimento das Ciências Humanas, como a necessidade de estimular linhas de pesquisa integradoras de projetos; compreender a especificidade da área com suas metodologias de divulgação científica e temporalidades; valorizar e incentivar redes de pesquisa estimulando o compartilhamento de competências; desenvolver espaços de convívio entre os pesquisadores. A pró-reitora de pesquisa da Unesp, Maria José Soares Mendes Giannini, pontua, na apresentação da obra, que a pesquisa em Ciências Humanas, desenvolve-se, em geral, a partir do esforço individual de um professor, que isolado, dedica-se à sua investigação. No entanto, afirma ela, as pesquisas dessa área do saber podem e devem estar abertas para novos modos de organização: “Um projeto de pesquisa, por exemplo, pode envolver vários professores de diferentes universidades e o compartilhamento de informações em um fórum on-line. Um professor pode colaborar com um colega de outra área de estudo para ter uma perspectiva alternativa sobre seu próprio objeto de pesquisa”. Entre os temas abordados no livro estão o caráter interdisciplinar da pesquisa em Ciências Humanas, pesquisa em grupo e “produtivismo”, a experiência de uma rede de pesquisa e reflexões sobre a prática interdisciplinar, entre outros.

Estudos sobre as instituições participativas: o debate sobre sua efetividade

The main purpose of this article is to introduce the debate on the effectiveness of the participatory institutions in Brazil. At first, it will bring the very early debate about participatory democracy and deliberative democracy. Afterwards, the article will show how international literature impacts studies on the democratic transition in Brazil, trying to delineate the borders, limitations and advances of Brazilian literature about the theme. In the last part, the main topics of debate on the effectiveness of participatory institutions - methods and instruments of evaluation - will be pointed out