Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera).

2014

Nomuraea rileyi (Hypocreales: Clavicipitaceae) in Helicoverpa armigera (Lepidoptera: Noctuidae) Larvae in Brazil.

2015

Comparison of biology between Helicoverpa zea and Helicoverpa armigera (Lepidoptera: Noctuidae) reared on artificial diets.

The objective of this study was to compare growth and development of Helicoverpa zea Boddie and Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) larvae that were feeding on an artificial diet. Neonate larvae of H. zea and H. armigera were collected in maize fields cultivated at the Brazilian Agricultural Research Corporation, Embrapa Maize & Sorghum, in Sete Lagoas (Minas Gerais) and in farmers? cotton fields in Luís Eduardo Magalhães (Bahia), respectively. Bioassays were conducted in the laboratory using individual larvae in 50 mL plastic cups fed a white bean?based artificial diet and maintained at a temperature of 26 ± 2 °C and a relative humidity of 47 ± 10%. The following larval biological parameters were evaluated: number and duration of instars, survival of larval instars and pupae, larval biomass, larval head capsule size, and larval length. An adaptation index was computed for comparison of development of the 2 species. Significant differences were observed between the species for all variables except for the number of instars and pupal survival. Although H. armigera larvae developed faster and were smaller than H. zea larvae, the diet tested can be considered adequate for rearing both species in the laboratory.

Juvenile-mature genetic correlations in Pinus taeda clones propagated via somatic embryogenesis.

Objetivou-se com o presente trabalho, estimar a correlação genética entre idades de seleção (juvenil-adulta) e eficiência da seleção precoce para as características altura, diâmetro e volume de indivíduos de famílias de Pinus taeda propagados via embriogênese somática. O estudo foi realizado por meio de análise genético-estatística pelo procedimento de estimação de componentes de variância (Reml) e de predição de valores genéticos (Blup), usando-se o software Selegen-Reml/Blup. As correlações genéticas entre idades juvenis e idade de rotação foram realizadas aplicando o modelo linear desenvolvido por Lambeth (1980). Segundo os resultados do modelo estabelecido, a seleção precoce pode ser realizada em clones de Pinus taeda com alta eficiência de seleção. As idades de 4 a 6 anos são suficientes para selecionar clones de Pinus taeda propagados via embriogênese somática para colheita aos 8 e 12 anos e, as idades de 6 a 10 anos são suficientes para selecionar para colheita aos 20 anos. De acordo com as estimativas de correlação genotípicaa partir dos ambientes, a seleção de clones de Pinus taeda propagados via embriogênese somática deve ser praticada de forma específica para cada ambiente. Pode-se realizar a seleção de clones considerando o diâmetro, visto a alta correlação observada entre volume e diâmetro.

Life-history traits of Spodoptera frugiperda populations exposed to low-dose Bt maize.

Exposure to Bacillus thuringiensis (Bt) toxins in low- and moderate-dose transgenic crops may induce sublethal effects and increase the rate of Bt resistance evolution, potentially compromising control efficacy against target pests. We tested this hypothesis using the fall armyworm Spodoptera frugiperda, a major polyphagous lepidopteran pest relatively tolerant to Bt notorious for evolving field-relevant resistance to single-gene Bt maize. Late-instar larvae were collected from Bt Cry1Ab and non-Bt maize fields in five locations in Brazil, and their offspring was compared for survival, development, and population growth in rearing environment without and with Cry1Ab throughout larval development. Larval survival on Cry1Ab maize leaves varied from 20 to 80% among the populations. Larvae reared on Cry1Ab maize had seven-day delay in development time in relation to control larvae, and such delay was shorter in offspring of armyworms from Cry1Ab maize. Population growth rates were 50?70% lower for insects continuously exposed to Cry1Ab maize relative to controls, showing the population-level effect of Cry1Ab, which varied among the populations and prior exposure to Cry1Ab maize in the field. In three out of five populations, armyworms derived from Bt maize reared on Cry1Ab maize showed higher larval weight, faster larval development and better reproductive performance than the armyworms derived from non-Bt maize, and one of these populations showed better performance on both Cry1Ab and control diets, indicating no fitness cost of the resistance trait. Altogether, these results indicate that offspring of armyworms that developed on field-grown, single-gene Bt Cry1Ab maize had reduced performance on Cry1Ab maize foliage in two populations studied, but in other three populations, these offspring had better overall performance on the Bt maize foliage than that of the armyworms from non-Bt maize fields, possibly because of Cry1Ab resistance alleles in these populations. Implications of these findings for resistance management of S. frugiperda in Bt crops are discussed.

Citrus HLB x Potato "Zebra Chip" in Brazil: Attempting to transmit citrus Candidatus liberibacter spp (asiaticus and americanus) to potato plants.

2009

Nova ocorrência da cochonilha-do-carmim em Opuntia spp. (Cactaceae) em Santa Catarina, Brasil.

2009

Bioecologia, monitoramento e controle de Drosophila suzukii na cultura do morangueiro.

2015

Exigências térmicas de desenvolvimento do psilídeo-de-concha Glycaspis brimblecombei (Hemiptera: Psillydae) em laboratório.

2009

Introdução de gramíneas visando resistência a cigarrinha-das-pastagens no Estado do Acre.

Devido a Brachiaria decumbens ser a gramínea de maior preferência para a implantação de pastagens nos últimos 10 anos no Estado do Acre, os problemas com ataques severos da cigarrinha-das-pastagens estão se intensificandonos últimos 3 anos. Isto evidencia a necessidade de que sejam encontradas outras opções de gramíneas resistentes e/ou tolerantes para a formação ou reforma de áreas de pastagens efetuando-se, desta forma, um controle integrado da praga.

Longevidade de adultos do psilídeo-de-concha Glycaspis brimblecombei (Hemiptera: Psillydae) em gaiola de criação em condição de laboratório.

2009

Guia para a identificação e monitoramento de pragas e seus inimigos naturais em morangueiro.

2015

METODOLOGIAS para monitoramento da vespa-da-madeira visando o planejamento das ações de controle: pínus.

2016

Natural parasitism by Trichogramma spp. in agroecosystems of the Mid-­North, Brazil.

The micro-­hymenopterans of genus Trichogramma are eggs parasitoids used in the biological control of lepidopteran pests. The objectives of this study was to record the interaction of species of Trichogramma and their hosts on crops in the Mid-­North, in the states of Maranhão and Piauí, Brazil.

Amblyseius largoensis in controlling red palm mite under semi-field conditions.

The objective of this work was to evaluate the efficiency of two Amblyseius largoensis (Acari:Phytoseiidae) populations in controlling Raoiella indica (Acari: Tenuipalpidae). The treatments were: release of A. largoensis from the island of La Réunion; release of A. largoensis from the state of Roraima, Brazil; and a control, without predator release. Initially, 20 predators were released per plant; three other releases were done at a rate of ten adults per plant, at 46, 135, and 156 days after the first release. The population densities were estimated every 20 days, during six months. Both A. largoensis populations evaluated are not sufficiently efficient to control the R. indica population.