A new kind of potassium sensor based on capacitance measurement of mimic membrane

A novel type of potassium sensor based on the capacitance change of valinomycin-incorporated bilayer supported on a gold electrode has been developed and characterized. The lipid membrane was Formed by painted method and monitored simultaneously by capacitance variation. The capacitance of the electrode-supported membrane was found to be modulated by different concentrations of K+. Investigating the capacitance change allows a simple and specific technique for the measurement of potassium ion in solution. Especially, the homemade capacitance meter is, to our knowledge, used to monitor the bilayer membrane formation and detect K+ for the first time. It has been proved that this capacitance measurement is a very useful technique because it is simple and sensitive compared to the other methods.

Characterization of multilayer film using electrochemical impedance spectroscopy

A new multilayer film fabricated based on electrostatic attraction in this laboratory was firstly characterized by the electrochemical impedance spectroscopy. The relationship between the charge-transfer resistance and double-layer capacitance with the number of layers was obtained through analyzing the impedance data. It demonstrated that the multilayer film showed a unique structure with the film growth. Compared to other electrochemical methods, the electrochemical impedance spectroscopy was proved to be a very sensitive and useful technique for characterizing the multilayer films.

Study on Ordered Assembly of SnO2 Nonoparticles-Arachidic Acid Composite LB Films

The hydrosol of SnO2 nanoparticles (NP) have been prepared by colloid chemistry method. The composite LB monolayer and multilayer of SnO2 NP-AA have been obtained by LB technique at the gas-liquid interface of the hydrosol subphase. The structures of the monolayer and multilayer were characterized by IR, UV-Vis, small angle X-ray diffraction spectroscopy and TEM technique, The results indicate that the coverage of SnO2 NP at the composite monolayer's surface is high and the sites of SnO2 NP are similar. The multilayer has good periodic structure.

A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica

A simple method was developed for extracting DNA from brown algae Laminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol ( 24: 1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 mu g g(-1) (wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A(260)/A(280), which was about 1.7 - 1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte in Laminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay (dot-ELISA), indirect ELISA and Western blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Western blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5 similar to 6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards ("marker") so that the alterations of protease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10(4) cell/mL in the indirect ELISA, while 10(5) cell/mL in the dot-ELISA.

Preliminary analysis of grain-size populations with environmentally sensitive terrigenous components in marginal sea setting

Terrigenous components were separated from the bulk sediment of Core A7 from the Okinawa Trough and Core A37 from the Ryukru Trench and grain-size distributions of these sub-samples were analyzed. Based upon an analysis of the grain-size data of the two sedimentary sequences, grain-size populations are identified to be sensitive to sedimentary environmental changes. The modal values and size ranges of the two main grain-size populations in Core A7 are evidently different from those of Core A37, indicating the spatial variability of sediment sources and transport processes between the two places. The downcore variations in the content of the environmentally sensitive grain-size populations reveal that during the accumulation of sedimentary material the environment remained relatively stable at the site where Core A7 was collected, except for the apparent events for the formation of two turbidite layers and a volcanic ash layer. However, the sedimentary sequence of Core A37 shows six sedimentary cycles, indicating a highly variable sedimentary environment at this location.

基于C8051的运动控制卡研制和应用

本文针对排爆机器人手臂多关节联动控制的需要,开发了一种具有高集成度的基于C8051单片机和CAN总线的运动控制卡。介绍了运动控制卡的原理及实施方案,同时给出了运动控制卡在机器人平台的应用实例。在排爆机器人平台上的应用表明该运动控制卡具有较高的精度及很好的可靠性和实时性。

可重构装配系统开放式组织结构的研究

为了克服传统装配线的不足,在基于功能对象的产品族建模基础上,提出了主干线和装配模块相结合的可重构装配系统开放式组织结构。这种分形相似的组织结构具有能力柔性、鲁棒性、功能扩展能力等特点,并能通过模块的局部调整快速响应市场的需求。利用网络法,针对鲁棒性和增容性问题,提出了相应的重构优化算法。

自主移动机器人足球比赛视觉定位方法综述

综述了RoboCup足球赛中全自主移动机器人基于视觉的定位技术,包括机器人自定位和多机器人协作物体定位.介绍了定位技术的发展情况与分类.从机器人环境构建形式的不同以及先验位姿和概率方法的应用与否等方面,系统地分析和比较了各种自定位方法.对于多机器人协作物体定位,阐述了静态方法和动态跟踪方法.总结了定位过程中需要重点研究的传感器模型构建、图像处理、特征匹配以及协作过程涉及的相关问题.最后就视觉定位存在的问题和技术发展趋势进行了讨论.

外科手术计算机辅助导航技术

外科手术计算机辅助导航即利用计算机图形图像技术对放射影像学资料进行处理 ,重建二维或三维的医学图像模型 ,同时结合各种空间定位技术 ,在医师的双眼、手术工具及患者的头部之间建立一个实时的环路 ,实现手术过程中器械位置的实时或准实时显示。我们综述了外科手术计算机辅助导航系统的发展历史和研究现状 (重点阐述了其系统结构和关键技术 ,包括空间定位技术、图像处理与显示技术、系统配准技术、头部定位技术等 (最后给出了手术导航系统的发展趋势

自由曲面透明体的非接触厚度测量

针对自由曲面透明体厚度测量,提出一种基于结构光机理的3D 视觉测量方法和系统结构,介绍了该方法的基本原理和测量系统实现技术,并给出了部分实验,证实了该方法的有效性。

微型遥控潜水器视频监控系统

介绍了一种微型遥控潜水器视频监控系统,包括硬件装置及基于软件技术的视频数据处理技术。硬件装置完成视频信号的采集、传输、显示。视频数据处理技术解决了视频信号的捕获、存储、画面质量调节等。该系统已经在微型遥控潜水器中应用,效果良好。

柔性制造单元的智能调度系统

本文基于递阶控制原理提出了柔性制造单元的一种新的计划与调度方法,并综合采用了理论分析、专家系统技术和仿真技术,建立了一个智能调度系统原型.另外,在制造单元调度问题的描述上采用了状态方程形式,从系统的观点来研究调度问题.并在此基础上建立了仿真模块,对单元的加工过程进行了仿真实验.

数控自动编程专家系统

本文简要地介绍了数控自动编程专家系统.其中包括:专家系统知识表示的形式;分层次的黑板结构;前向推理求解策略和相应的解释功能;系统针对不同类型的曲线组合,采用不同的独立的知识源(KS)进行处理.由于在知识的处理上采用编码技术,在前向推理求解策略中使用启发信息和“剪技”技术,提高了系统的时空效率.系统中的规划程序能自动规划切削路径.输出供数控车床使用的 NC 代码,并可在显示屏上进行图形显示和切削仿真.目前原型系统已经在 IBM-PC 和 Sun3/60计算机上利用FORTRAN 语言实现.

基于LQS的基本矩阵计算方法

基本矩阵作为分析两视图对极几何的有力工具,在视觉领域中占用重要的地位。分析了传统鲁棒方法在基本矩阵的求解问题中存在的不足,引入了稳健回归分析中的LQS方法,并结合Bucket分割技术,提出一种鲁棒估计基本矩阵的新方法,克服了RANSAC方法和LMedS方法的缺陷。模拟数据和真实图像实验结果表明,本文方法具有更高的鲁棒性和精确度。